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Conserved domains on  [gi|1655142367|ref|WP_137626846|]
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GNAT family N-acetyltransferase [Lactiplantibacillus pingfangensis]

Protein Classification

GNAT family protein( domain architecture ID 106742)

GNAT (Gcn5-related N-acetyltransferase) family protein similar to N-acetyltransferases that catalyze the transfer of an acetyl group from acetyl-CoA to a substrate

PubMed:  15581578

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NAT_SF super family cl17182
N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of ...
 1-150 9.01e-26

N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase which catalyze the transfer of an acetyl group to a substrate. The mechanism is an ordered Bi-Bi ternary complex kinetic mechanism for most GNATs: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and then CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/ph enylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


The actual alignment was detected with superfamily member PRK09831:

Pssm-ID: 473072  Cd Length: 147  Bit Score: 95.41  E-value: 9.01e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367   1 MKIRNYQSTDILAIRRLFKRTVLQINAADYTPQQLTAWIGPDNEQvaaaWQKSLAAHTALVAVQADQIVGFadMSASG-Y 79
Cdd:PRK09831    1 IQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQIDESR----WKEKLAKSQVRVAVINAQPVGF--ITCIEhY 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1655142367  80 LDRLYVDADCQRQGIATTLVAELESQVdcERYTTAASITARPFFESRGYQVVQAQCVVRAGIQMTNYLMAK 150
Cdd:PRK09831   75 IDMLFVDPEYTRRGVASALLKPLIKSE--SELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRY 143
 
Name Accession Description Interval E-value
PRK09831 PRK09831
GNAT family N-acetyltransferase;
1-150 9.01e-26

GNAT family N-acetyltransferase;


Pssm-ID: 182099  Cd Length: 147  Bit Score: 95.41  E-value: 9.01e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367   1 MKIRNYQSTDILAIRRLFKRTVLQINAADYTPQQLTAWIGPDNEQvaaaWQKSLAAHTALVAVQADQIVGFadMSASG-Y 79
Cdd:PRK09831    1 IQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQIDESR----WKEKLAKSQVRVAVINAQPVGF--ITCIEhY 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1655142367  80 LDRLYVDADCQRQGIATTLVAELESQVdcERYTTAASITARPFFESRGYQVVQAQCVVRAGIQMTNYLMAK 150
Cdd:PRK09831   75 IDMLFVDPEYTRRGVASALLKPLIKSE--SELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRY 143
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 27-150 4.01e-19

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 77.70  E-value: 4.01e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  27 AADYTPQQLTAWigpDNEQVAAAWQKSLA--AHTALVAVQADQIVGFADMSASGYLDRLYVDADCQRQGIATTLVAELES 104
Cdd:pfam13673   2 APDYSEEGIETF---YEFISPEALRERIDqgEYFFFVAFEGGQIVGVIALRDRGHISLLFVDPDYQGQGIGKALLEAVED 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1655142367 105 QVDCERYTTA-----ASITARPFFESRGYQVVQAQCvVRAGIQMTNylMAK 150
Cdd:pfam13673  79 YAEKDGIKLSeltvnASPYAVPFYEKLGFRATGPEQ-EFNGIRFVP--MEK 126
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-151 3.32e-15

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 68.48  E-value: 3.32e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367   1 MKIRNYQSTDILAIRRLFKRTVLQINAadytpqqlTAWIGPDNEQVAAAW--QKSLAAHTALVAVQADQIVGFADMSA-- 76
Cdd:COG1247     2 MTIRPATPEDAPAIAAIYNEAIAEGTA--------TFETEPPSEEEREAWfaAILAPGRPVLVAEEDGEVVGFASLGPfr 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  77 --SGY----LDRLYVDADCQRQGIATTLVAELESQVDCERYTT------AASITARPFFESRGYQVV--QAQCVVRAGIQ 142
Cdd:COG1247    74 prPAYrgtaEESIYVDPDARGRGIGRALLEALIERARARGYRRlvavvlADNEASIALYEKLGFEEVgtLPEVGFKFGRW 153


                  ....*....
gi 1655142367 143 MTNYLMAKP 151
Cdd:COG1247   154 LDLVLMQKR 162
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 60-105 4.19e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 44.57  E-value: 4.19e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1655142367  60 LVAVQADQIVGFADMSAS------GYLDRLYVDADCQRQGIATTLVAELESQ 105
Cdd:cd04301     2 LVAEDDGEIVGFASLSPDgsggdtAYIGDLAVLPEYRGKGIGSALLEAAEEE 53
 
Name Accession Description Interval E-value
PRK09831 PRK09831
GNAT family N-acetyltransferase;
1-150 9.01e-26

GNAT family N-acetyltransferase;


Pssm-ID: 182099  Cd Length: 147  Bit Score: 95.41  E-value: 9.01e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367   1 MKIRNYQSTDILAIRRLFKRTVLQINAADYTPQQLTAWIGPDNEQvaaaWQKSLAAHTALVAVQADQIVGFadMSASG-Y 79
Cdd:PRK09831    1 IQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQIDESR----WKEKLAKSQVRVAVINAQPVGF--ITCIEhY 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1655142367  80 LDRLYVDADCQRQGIATTLVAELESQVdcERYTTAASITARPFFESRGYQVVQAQCVVRAGIQMTNYLMAK 150
Cdd:PRK09831   75 IDMLFVDPEYTRRGVASALLKPLIKSE--SELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRY 143
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 27-150 4.01e-19

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 77.70  E-value: 4.01e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  27 AADYTPQQLTAWigpDNEQVAAAWQKSLA--AHTALVAVQADQIVGFADMSASGYLDRLYVDADCQRQGIATTLVAELES 104
Cdd:pfam13673   2 APDYSEEGIETF---YEFISPEALRERIDqgEYFFFVAFEGGQIVGVIALRDRGHISLLFVDPDYQGQGIGKALLEAVED 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1655142367 105 QVDCERYTTA-----ASITARPFFESRGYQVVQAQCvVRAGIQMTNylMAK 150
Cdd:pfam13673  79 YAEKDGIKLSeltvnASPYAVPFYEKLGFRATGPEQ-EFNGIRFVP--MEK 126
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-151 3.32e-15

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 68.48  E-value: 3.32e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367   1 MKIRNYQSTDILAIRRLFKRTVLQINAadytpqqlTAWIGPDNEQVAAAW--QKSLAAHTALVAVQADQIVGFADMSA-- 76
Cdd:COG1247     2 MTIRPATPEDAPAIAAIYNEAIAEGTA--------TFETEPPSEEEREAWfaAILAPGRPVLVAEEDGEVVGFASLGPfr 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  77 --SGY----LDRLYVDADCQRQGIATTLVAELESQVDCERYTT------AASITARPFFESRGYQVV--QAQCVVRAGIQ 142
Cdd:COG1247    74 prPAYrgtaEESIYVDPDARGRGIGRALLEALIERARARGYRRlvavvlADNEASIALYEKLGFEEVgtLPEVGFKFGRW 153


                  ....*....
gi 1655142367 143 MTNYLMAKP 151
Cdd:COG1247   154 LDLVLMQKR 162
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
3-151 7.36e-15

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 67.03  E-value: 7.36e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367   3 IRNYQSTDILAIRRLFKRtvlqinaadytpqqltAWIGPDNEQVAAAWQKSLAAHTALVAVQADQIVGFADMSAS----- 77
Cdd:COG3153     1 IRPATPEDAEAIAALLRA----------------AFGPGREAELVDRLREDPAAGLSLVAEDDGEIVGHVALSPVdidge 64

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  78 ---GYLDRLYVDADCQRQGIATTLVAELESQV---DCERYTTAASITARPFFESRGYQVVQAQCVvraGIQMTNYLMAKP 151
Cdd:COG3153    65 gpaLLLGPLAVDPEYRGQGIGRALMRAALEAArerGARAVVLLGDPSLLPFYERFGFRPAGELGL---TLGPDEVFLAKE 141
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 1-136 7.34e-14

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 64.24  E-value: 7.34e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367   1 MKIRNYQSTDILAIRRLFKRTVLQINAADYtpqqltawigpdneqvaaawqkslaahtaLVAVQADQIVGFADMSAS--- 77
Cdd:COG1246     1 MTIRPATPDDVPAILELIRPYALEEEIGEF-----------------------------WVAEEDGEIVGCAALHPLded 51

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1655142367  78 -GYLDRLYVDADCQRQGIATTLVAELESQV---DCERYTTAASITARPFFESRGYQVVQAQCV 136
Cdd:COG1246    52 lAELRSLAVHPDYRGRGIGRRLLEALLAEArelGLKRLFLLTTSAAIHFYEKLGFEEIDKEDL 114
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 57-130 1.49e-11

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 56.69  E-value: 1.49e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  57 HTALVAVQADQIVGFADMSASGYLD-----RLYVDADCQRQGIATTLVAELE---SQVDCERYTTAASITARPFFESRGY 128
Cdd:pfam13508   3 GRFFVAEDDGKIVGFAALLPLDDEGalaelRLAVHPEYRGQGIGRALLEAAEaaaKEGGIKLLELETTNRAAAFYEKLGF 82


                  ..
gi 1655142367 129 QV 130
Cdd:pfam13508  83 EE 84
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 43-144 1.75e-10

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 55.44  E-value: 1.75e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  43 NEQVAAAWqKSLAAHTALVAVQADQIVGFADMS----ASGYLDRLYVDADCQRQGIATTLVAELES----------QVDC 108
Cdd:COG0454    21 DAELKAME-GSLAGAEFIAVDDKGEPIGFAGLRrlddKVLELKRLYVLPEYRGKGIGKALLEALLEwarergctalELDT 99

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1655142367 109 ERYTTAasitARPFFESRGYQVVQAQCV-VRAGIQMT 144
Cdd:COG0454   100 LDGNPA----AIRFYERLGFKEIERYVAyVGGEFEKE 132
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 49-128 1.13e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 52.91  E-value: 1.13e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  49 AWQKSLAAHTALVAVQADQIVGFA------DMSASGYLDRLYVDADCQRQGIATTLVAELES---QVDCER---YTTAAS 116
Cdd:pfam00583  25 EDWDEDASEGFFVAEEDGELVGFAslsiidDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEwarERGCERiflEVAADN 104

                          90
                  ....*....|..
gi 1655142367 117 ITARPFFESRGY 128
Cdd:pfam00583 105 LAAIALYEKLGF 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 68-131 2.13e-08

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 48.88  E-value: 2.13e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  68 IVGFADMSASGYLDRLYVDADCQRQGIATTLVAELESQV---DCER---YTTAASITARPFFESRGYQVV 131
Cdd:COG0456     4 LLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERArerGARRlrlEVREDNEAAIALYEKLGFEEV 73
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 60-105 4.19e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 44.57  E-value: 4.19e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1655142367  60 LVAVQADQIVGFADMSAS------GYLDRLYVDADCQRQGIATTLVAELESQ 105
Cdd:cd04301     2 LVAEDDGEIVGFASLSPDgsggdtAYIGDLAVLPEYRGKGIGSALLEAAEEE 53
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
55-131 3.69e-06

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 43.63  E-value: 3.69e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1655142367  55 AAHtaLVAVQADQIVG----FADMSASGYLDRLYVDADCQRQGIATTLVAELESQVDCERYTTA---ASITARPFFESRG 127
Cdd:COG2153    34 ARH--LLAYDDGELVAtarlLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRIvlsAQAHAVGFYEKLG 111


                  ....
gi 1655142367 128 YQVV 131
Cdd:COG2153   112 FVPV 115
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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