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Conserved domains on  [gi|19483909|gb|AAH23469|]
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Rhbdf1 protein, partial [Mus musculus]

Protein Classification

inactive rhomboid protein( domain architecture ID 10323667)

inactive rhomboid protein regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation; does not exhibit any protease activity on its own

CATH:  1.20.1540.10
MEROPS:  S54
PubMed:  21439629|22035660|24099005
SCOP:  4000471

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Rhomboid pfam01694
Rhomboid family; This family contains integral membrane proteins that are related to ...
 413-556 3.14e-34

Rhomboid family; This family contains integral membrane proteins that are related to Drosophila rhomboid protein. Members of this family are found in bacteria and eukaryotes. Rhomboid promotes the cleavage of the membrane-anchored TGF-alpha-like growth factor Spitz, allowing it to activate the Drosophila EGF receptor. Analysis has shown that Rhomboid-1 is an intramembrane serine protease (EC:3.4.21.105). Parasite-encoded rhomboid enzymes are also important for invasion of host cells by Toxoplasma and the malaria parasite.


:

Pssm-ID: 426384  Cd Length: 147  Bit Score: 126.95  E-value: 3.14e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19483909   413 PEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPY-RAEVGPAGSQFG 491
Cdd:pfam01694   1 PVQPGQLWRLITSMFLHAGWLHLLFNMLALLFFGGPLERILGSVRFLLLYLLSGIAGSLLSYLFSPLsTPSVGASGAIFG 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 19483909   492 ILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPW--IDNFAHISGFVSGLFLSFAFLPYI 556
Cdd:pfam01694  81 LLGALLVLGPRNRILLFGLIGALLALLLFILLNLVLGLLPGngVSNLAHLGGLLVGLLLGFILLRRP 147
Rhomboid_SP super family cl13970
Rhomboid serine protease; This domain family is found in eukaryotes, and is approximately 210 ...
 1-50 1.33e-24

Rhomboid serine protease; This domain family is found in eukaryotes, and is approximately 210 amino acids in length. The family is found in association with pfam01694. Rhomboid is a seven-transmembrane spanning protein that resides in the Golgi and acts as a serine protease to cleave Spitz.


The actual alignment was detected with superfamily member pfam12595:

Pssm-ID: 463639  Cd Length: 193  Bit Score: 101.49  E-value: 1.33e-24
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 19483909     1 GQRRSFTPASFLEEDMVDFPDELDTSFFAREGvLHEEMSTYPDEVFESPS 50
Cdd:pfam12595 145 RRRRSFTPPSFLEEDTVDFADELDTSFFSKDD-LHEELSSMADEVFESPS 193
 
Name Accession Description Interval E-value
Rhomboid pfam01694
Rhomboid family; This family contains integral membrane proteins that are related to ...
 413-556 3.14e-34

Rhomboid family; This family contains integral membrane proteins that are related to Drosophila rhomboid protein. Members of this family are found in bacteria and eukaryotes. Rhomboid promotes the cleavage of the membrane-anchored TGF-alpha-like growth factor Spitz, allowing it to activate the Drosophila EGF receptor. Analysis has shown that Rhomboid-1 is an intramembrane serine protease (EC:3.4.21.105). Parasite-encoded rhomboid enzymes are also important for invasion of host cells by Toxoplasma and the malaria parasite.


Pssm-ID: 426384  Cd Length: 147  Bit Score: 126.95  E-value: 3.14e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19483909   413 PEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPY-RAEVGPAGSQFG 491
Cdd:pfam01694   1 PVQPGQLWRLITSMFLHAGWLHLLFNMLALLFFGGPLERILGSVRFLLLYLLSGIAGSLLSYLFSPLsTPSVGASGAIFG 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 19483909   492 ILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPW--IDNFAHISGFVSGLFLSFAFLPYI 556
Cdd:pfam01694  81 LLGALLVLGPRNRILLFGLIGALLALLLFILLNLVLGLLPGngVSNLAHLGGLLVGLLLGFILLRRP 147
Rhomboid_SP pfam12595
Rhomboid serine protease; This domain family is found in eukaryotes, and is approximately 210 ...
 1-50 1.33e-24

Rhomboid serine protease; This domain family is found in eukaryotes, and is approximately 210 amino acids in length. The family is found in association with pfam01694. Rhomboid is a seven-transmembrane spanning protein that resides in the Golgi and acts as a serine protease to cleave Spitz.


Pssm-ID: 463639  Cd Length: 193  Bit Score: 101.49  E-value: 1.33e-24
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 19483909     1 GQRRSFTPASFLEEDMVDFPDELDTSFFAREGvLHEEMSTYPDEVFESPS 50
Cdd:pfam12595 145 RRRRSFTPPSFLEEDTVDFADELDTSFFSKDD-LHEELSSMADEVFESPS 193
GlpG COG0705
Membrane-associated serine protease, rhomboid family [Posttranslational modification, protein ...
 418-554 8.08e-21

Membrane-associated serine protease, rhomboid family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440469 [Multi-domain]  Cd Length: 189  Bit Score: 90.30  E-value: 8.08e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19483909 418 QFYRLWLSLFLHAGILHCLvsvcFQMTVL----RDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAE--VGPAGSQFG 491
Cdd:COG0705  42 ELWRLLTSMFLHGGFLHLL----FNMLALwvfgPLLERRLGSKRFLLLYLLSGLGGGLLQLLFSPGSGYplVGASGAIFG 117

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 19483909 492 ILACLFV-ELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPW--IDNFAHISGFVSGLFLSFAFLP 554
Cdd:COG0705 118 LLGALLVlGPRRRVLLLFIPIPALLFLLVWLLLGLLFGLLGGggIAWEAHLGGLLAGLLLALLLRK 183
PTZ00101 PTZ00101
rhomboid-1 protease; Provisional
 418-546 1.89e-13

rhomboid-1 protease; Provisional


Pssm-ID: 185445  Cd Length: 278  Bit Score: 71.04  E-value: 1.89e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19483909  418 QFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNL--ASAIFLPYRaeVGPAGSQFGILAC 495
Cdd:PTZ00101 101 EIHRLILPIFLHANIFHTFFNVFFQLRMGFTLEKNYGIVKIIILYFLTGIYGNIlsSSVTYCPIK--VGASTSGMGLLGI 178

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 19483909  496 LFVELFQSWQILARPWRAFFKLLAVVL--FLFAFGLL-PWIDNFAHISGFVSGL 546
Cdd:PTZ00101 179 VTSELILLWHVIRHRERVVFNIIFFSLisFFYYFTFNgSNIDHVGHLGGLLSGI 232
 
Name Accession Description Interval E-value
Rhomboid pfam01694
Rhomboid family; This family contains integral membrane proteins that are related to ...
 413-556 3.14e-34

Rhomboid family; This family contains integral membrane proteins that are related to Drosophila rhomboid protein. Members of this family are found in bacteria and eukaryotes. Rhomboid promotes the cleavage of the membrane-anchored TGF-alpha-like growth factor Spitz, allowing it to activate the Drosophila EGF receptor. Analysis has shown that Rhomboid-1 is an intramembrane serine protease (EC:3.4.21.105). Parasite-encoded rhomboid enzymes are also important for invasion of host cells by Toxoplasma and the malaria parasite.


Pssm-ID: 426384  Cd Length: 147  Bit Score: 126.95  E-value: 3.14e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19483909   413 PEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPY-RAEVGPAGSQFG 491
Cdd:pfam01694   1 PVQPGQLWRLITSMFLHAGWLHLLFNMLALLFFGGPLERILGSVRFLLLYLLSGIAGSLLSYLFSPLsTPSVGASGAIFG 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 19483909   492 ILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPW--IDNFAHISGFVSGLFLSFAFLPYI 556
Cdd:pfam01694  81 LLGALLVLGPRNRILLFGLIGALLALLLFILLNLVLGLLPGngVSNLAHLGGLLVGLLLGFILLRRP 147
Rhomboid_SP pfam12595
Rhomboid serine protease; This domain family is found in eukaryotes, and is approximately 210 ...
 1-50 1.33e-24

Rhomboid serine protease; This domain family is found in eukaryotes, and is approximately 210 amino acids in length. The family is found in association with pfam01694. Rhomboid is a seven-transmembrane spanning protein that resides in the Golgi and acts as a serine protease to cleave Spitz.


Pssm-ID: 463639  Cd Length: 193  Bit Score: 101.49  E-value: 1.33e-24
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 19483909     1 GQRRSFTPASFLEEDMVDFPDELDTSFFAREGvLHEEMSTYPDEVFESPS 50
Cdd:pfam12595 145 RRRRSFTPPSFLEEDTVDFADELDTSFFSKDD-LHEELSSMADEVFESPS 193
GlpG COG0705
Membrane-associated serine protease, rhomboid family [Posttranslational modification, protein ...
 418-554 8.08e-21

Membrane-associated serine protease, rhomboid family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440469 [Multi-domain]  Cd Length: 189  Bit Score: 90.30  E-value: 8.08e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19483909 418 QFYRLWLSLFLHAGILHCLvsvcFQMTVL----RDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAE--VGPAGSQFG 491
Cdd:COG0705  42 ELWRLLTSMFLHGGFLHLL----FNMLALwvfgPLLERRLGSKRFLLLYLLSGLGGGLLQLLFSPGSGYplVGASGAIFG 117

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 19483909 492 ILACLFV-ELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPW--IDNFAHISGFVSGLFLSFAFLP 554
Cdd:COG0705 118 LLGALLVlGPRRRVLLLFIPIPALLFLLVWLLLGLLFGLLGGggIAWEAHLGGLLAGLLLALLLRK 183
PTZ00101 PTZ00101
rhomboid-1 protease; Provisional
 418-546 1.89e-13

rhomboid-1 protease; Provisional


Pssm-ID: 185445  Cd Length: 278  Bit Score: 71.04  E-value: 1.89e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19483909  418 QFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNL--ASAIFLPYRaeVGPAGSQFGILAC 495
Cdd:PTZ00101 101 EIHRLILPIFLHANIFHTFFNVFFQLRMGFTLEKNYGIVKIIILYFLTGIYGNIlsSSVTYCPIK--VGASTSGMGLLGI 178

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 19483909  496 LFVELFQSWQILARPWRAFFKLLAVVL--FLFAFGLL-PWIDNFAHISGFVSGL 546
Cdd:PTZ00101 179 VTSELILLWHVIRHRERVVFNIIFFSLisFFYYFTFNgSNIDHVGHLGGLLSGI 232
7TMR-DISM_7TM pfam07695
7TM diverse intracellular signalling; This entry represents the transmembrane region of the ...
 407-551 3.44e-03

7TM diverse intracellular signalling; This entry represents the transmembrane region of the 7TM-DISM (7TM Receptors with Diverse Intracellular Signalling Modules).


Pssm-ID: 429600 [Multi-domain]  Cd Length: 207  Bit Score: 39.18  E-value: 3.44e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19483909   407 LLPFLNPEVPDQF--YRLWLSLFLHAGILHCLvsvcFQMTVLrDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVg 484
Cdd:pfam07695  52 GFQYLWPNAPPWLnnKLLYLSLLLLLPFFALL----FARSFL-ELKKYLPRLLRLLLGLALLLALLLLLLPLFPYTLSL- 125

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 19483909   485 PAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFA--------FGLLP---WIDNFAHISGFVSGLFLSFA 551
Cdd:pfam07695 126 PLAQLLALLFILFLLLLGIIAWRKGYKPARYFLLAWLLLLIGalidilslLGLLPsnfFTNYLLQIGSALEVLLLSLA 203
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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