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Conserved domains on  [gi|32171383|sp|Q8C0Q9|]
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RecName: Full=Rap guanine nucleotide exchange factor 5; AltName: Full=Guanine nucleotide exchange factor for Rap1; AltName: Full=M-Ras-regulated Rap GEF; Short=MR-GEF

Protein Classification

DEP_Epac and RasGEF domain-containing protein( domain architecture ID 10858405)

protein containing domains DEP_Epac, REM, and RasGEF

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
574-813 4.97e-85

Guanine nucleotide exchange factor for Ras-like small GTPases;


:

Pssm-ID: 214539  Cd Length: 242  Bit Score: 270.66  E-value: 4.97e-85
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    574 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQ-GNGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 652
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRsKKSPSPLNLEAFIRRFNEVSNWVATEILKQTTPKDRAELLSK 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    653 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKM-KPPKIPFMP 731
Cdd:smart00147  81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIPFLG 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    732 LLLKDVTFIHEGNKTFL-DNLVNFEKLHMIADTVRTLRHCRTNqfgSDVSPKEQQELKSYVNHL-YVIDSQQALFELSHR 809
Cdd:smart00147 161 VLLKDLTFIDEGNPDFLeNGLVNFEKRRQIAEILREIRQLQSQ---PYNLRPNRSDIQSLLQQLlDHLDEEEELYQLSLK 237


                   ....
gi 32171383    810 LEPR 813
Cdd:smart00147 238 IEPR 241
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 33-156 6.49e-61

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


:

Pssm-ID: 239884  Cd Length: 125  Bit Score: 201.42  E-value: 6.49e-61
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383  33 AGRALRNIIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYV 112
Cdd:cd04437   1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKYQ 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*
gi 32171383 113 FYQFSSDECSYLY-CEFEREEEWQKGVKLLLELVHLIPARAGICD 156
Cdd:cd04437  81 FYRFSDDECSPAPlEKREAEEELQEAVTLLSQLGPDALLRMILRK 125
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 302-433 2.98e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


:

Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.98e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    302 RYVVVSGTPEKILEHLLndlhlaEVQHKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GEEENSDVPCRKRKV 380
Cdd:smart00229   2 GGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNRV 75

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 32171383    381 LHLVSQWISLYKDWLHEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 433
Cdd:smart00229  76 LNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
574-813 4.97e-85

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 270.66  E-value: 4.97e-85
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    574 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQ-GNGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 652
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRsKKSPSPLNLEAFIRRFNEVSNWVATEILKQTTPKDRAELLSK 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    653 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKM-KPPKIPFMP 731
Cdd:smart00147  81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIPFLG 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    732 LLLKDVTFIHEGNKTFL-DNLVNFEKLHMIADTVRTLRHCRTNqfgSDVSPKEQQELKSYVNHL-YVIDSQQALFELSHR 809
Cdd:smart00147 161 VLLKDLTFIDEGNPDFLeNGLVNFEKRRQIAEILREIRQLQSQ---PYNLRPNRSDIQSLLQQLlDHLDEEEELYQLSLK 237


                   ....
gi 32171383    810 LEPR 813
Cdd:smart00147 238 IEPR 241
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 574-809 2.02e-80

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 257.95  E-value: 2.02e-80
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383 574 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQG-NGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 652
Cdd:cd00155   1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDkNIHLSPNLERFIERFNNLSNWVASEILLCTNPKKRARLLSK 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383 653 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMK--PPKIPFM 730
Cdd:cd00155  81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVGpnPPCVPFL 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383 731 PLLLKDVTFIHEGNKTFLD-NLVNFEKLHMIADTVRTLRHCRTNQFgsdvSPKEQQELKSYVNHL-YVIDSQQALFELSH 808
Cdd:cd00155 161 GVYLKDLTFLHEGNPDFLEgNLVNFEKRRKIAEILREIRQLQSNSY----ELNRDEDILAFLWKLlELILNEDELYELSL 236


                .
gi 32171383 809 R 809
Cdd:cd00155 237 E 237
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 581-758 1.07e-68

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 224.78  E-value: 1.07e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383   581 DLALELMSFDWSLFNSIHEQELIYFTFSRQGNGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKFIKIAAHC 660
Cdd:pfam00617   1 ELARQLTLIEFELFRKIKPRELLGSAWSKKDKKENSPNIEAMIARFNKLSNWVASEILSEEDLKKRAKVIKKFIKIAEHC 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383   661 KAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKPPKIPFMPLLLKDVTFI 740
Cdd:pfam00617  81 RELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSASPPCIPFLGLYLTDLTFI 160

                         170
                  ....*....|....*....
gi 32171383   741 HEGNKTFL-DNLVNFEKLH 758
Cdd:pfam00617 161 EEGNPDFLeGGLINFEKRR 179
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 33-156 6.49e-61

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


Pssm-ID: 239884  Cd Length: 125  Bit Score: 201.42  E-value: 6.49e-61
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383  33 AGRALRNIIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYV 112
Cdd:cd04437   1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKYQ 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*
gi 32171383 113 FYQFSSDECSYLY-CEFEREEEWQKGVKLLLELVHLIPARAGICD 156
Cdd:cd04437  81 FYRFSDDECSPAPlEKREAEEELQEAVTLLSQLGPDALLRMILRK 125
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 302-433 2.98e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.98e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    302 RYVVVSGTPEKILEHLLndlhlaEVQHKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GEEENSDVPCRKRKV 380
Cdd:smart00229   2 GGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNRV 75

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 32171383    381 LHLVSQWISLYKDWLHEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 433
Cdd:smart00229  76 LNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 309-432 2.55e-18

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 81.69  E-value: 2.55e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383 309 TPEKILEHLLNDLHLAEvqhketETLLDDFLLTYTVFMTTDDLCQALLRHY-----SAKKYQGEEENSDVPCRKRkVLHL 383
Cdd:cd06224   1 TLEALIEHLTSTFDMPD------PSFVSTFLLTYRSFTTPTELLEKLIERYeiappENLEYNDWDKKKSKPIRLR-VLNV 73

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*....
gi 32171383 384 VSQWISLYKDWLHEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKI 432
Cdd:cd06224  74 LRTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKLLRKLLKL 122
DEP pfam00610
Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for ...
 49-116 3.58e-14

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit.


Pssm-ID: 459867  Cd Length: 71  Bit Score: 68.00  E-value: 3.58e-14
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 32171383    49 VKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSV-DQHLYFQDNYVFYQF 116
Cdd:pfam00610   4 LKDRRKHLKTYPNCFTGSEAVDWLMDNLE-IITREEAVELGQLLLDQGLIHHVgDKHGLFKDSYYFYRF 71
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 304-391 2.07e-11

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 61.17  E-value: 2.07e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383   304 VVVSGTPEKILEHLLNDLHLAEvqhketETLLDDFLLTYTVFMTTDDLCQALLRHY----SAKKYQGEEENSDV--PCRK 377
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRIMLD------DSFLSTFLLTYRSFTTPAELLELLIERYnippPLDLSSDSYWISKKtlPIRI 74

                          90
                  ....*....|....
gi 32171383   378 RkVLHLVSQWISLY 391
Cdd:pfam00618  75 R-VLSVLRHWVENY 87
DEP smart00049
Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in ...
 49-118 1.38e-10

Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in signalling proteins that contain PH, rasGEF, rhoGEF, rhoGAP, RGS, PDZ domains. DEP domain in Drosophila dishevelled is essential to rescue planar polarity defects and induce JNK signalling (Cell 94, 109-118).


Pssm-ID: 214489  Cd Length: 77  Bit Score: 58.06  E-value: 1.38e-10
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 32171383     49 VKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSVDQH--LYFQDNYVFYQFSS 118
Cdd:smart00049   7 LRDRKYFLKTYPNCFTGSELVDWLMDNLE-IIDREEAVHLGQLLLDEGLIHHVNGPnkHTFKDSKALYRFTT 77
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
574-813 4.97e-85

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 270.66  E-value: 4.97e-85
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    574 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQ-GNGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 652
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRsKKSPSPLNLEAFIRRFNEVSNWVATEILKQTTPKDRAELLSK 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    653 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKM-KPPKIPFMP 731
Cdd:smart00147  81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIPFLG 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    732 LLLKDVTFIHEGNKTFL-DNLVNFEKLHMIADTVRTLRHCRTNqfgSDVSPKEQQELKSYVNHL-YVIDSQQALFELSHR 809
Cdd:smart00147 161 VLLKDLTFIDEGNPDFLeNGLVNFEKRRQIAEILREIRQLQSQ---PYNLRPNRSDIQSLLQQLlDHLDEEEELYQLSLK 237


                   ....
gi 32171383    810 LEPR 813
Cdd:smart00147 238 IEPR 241
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 574-809 2.02e-80

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 257.95  E-value: 2.02e-80
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383 574 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQG-NGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 652
Cdd:cd00155   1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDkNIHLSPNLERFIERFNNLSNWVASEILLCTNPKKRARLLSK 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383 653 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMK--PPKIPFM 730
Cdd:cd00155  81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVGpnPPCVPFL 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383 731 PLLLKDVTFIHEGNKTFLD-NLVNFEKLHMIADTVRTLRHCRTNQFgsdvSPKEQQELKSYVNHL-YVIDSQQALFELSH 808
Cdd:cd00155 161 GVYLKDLTFLHEGNPDFLEgNLVNFEKRRKIAEILREIRQLQSNSY----ELNRDEDILAFLWKLlELILNEDELYELSL 236


                .
gi 32171383 809 R 809
Cdd:cd00155 237 E 237
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 581-758 1.07e-68

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 224.78  E-value: 1.07e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383   581 DLALELMSFDWSLFNSIHEQELIYFTFSRQGNGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKFIKIAAHC 660
Cdd:pfam00617   1 ELARQLTLIEFELFRKIKPRELLGSAWSKKDKKENSPNIEAMIARFNKLSNWVASEILSEEDLKKRAKVIKKFIKIAEHC 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383   661 KAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKPPKIPFMPLLLKDVTFI 740
Cdd:pfam00617  81 RELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSASPPCIPFLGLYLTDLTFI 160

                         170
                  ....*....|....*....
gi 32171383   741 HEGNKTFL-DNLVNFEKLH 758
Cdd:pfam00617 161 EEGNPDFLeGGLINFEKRR 179
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 33-156 6.49e-61

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


Pssm-ID: 239884  Cd Length: 125  Bit Score: 201.42  E-value: 6.49e-61
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383  33 AGRALRNIIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYV 112
Cdd:cd04437   1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKYQ 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*
gi 32171383 113 FYQFSSDECSYLY-CEFEREEEWQKGVKLLLELVHLIPARAGICD 156
Cdd:cd04437  81 FYRFSDDECSPAPlEKREAEEELQEAVTLLSQLGPDALLRMILRK 125
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 302-433 2.98e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 104.34  E-value: 2.98e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383    302 RYVVVSGTPEKILEHLLndlhlaEVQHKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GEEENSDVPCRKRKV 380
Cdd:smart00229   2 GGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNRV 75

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 32171383    381 LHLVSQWISLYKDWLHEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 433
Cdd:smart00229  76 LNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 309-432 2.55e-18

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 81.69  E-value: 2.55e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383 309 TPEKILEHLLNDLHLAEvqhketETLLDDFLLTYTVFMTTDDLCQALLRHY-----SAKKYQGEEENSDVPCRKRkVLHL 383
Cdd:cd06224   1 TLEALIEHLTSTFDMPD------PSFVSTFLLTYRSFTTPTELLEKLIERYeiappENLEYNDWDKKKSKPIRLR-VLNV 73

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*....
gi 32171383 384 VSQWISLYKDWLHEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKI 432
Cdd:cd06224  74 LRTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKLLRKLLKL 122
DEP cd04371
DEP domain, named after Dishevelled, Egl-10, and Pleckstrin, where this domain was first ...
 35-115 1.15e-15

DEP domain, named after Dishevelled, Egl-10, and Pleckstrin, where this domain was first discovered. The function of this domain is still not clear, but it is believed to be important for the membrane association of the signaling proteins in which it is present. New studies show that the DEP domain of Sst2, a yeast RGS protein is necessary and sufficient for receptor interaction.


Pssm-ID: 239836  Cd Length: 81  Bit Score: 72.37  E-value: 1.15e-15
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383  35 RALRNIIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSV-DQHLYFQDNYVF 113
Cdd:cd04371   1 DLVRIMLDSDSGVPIKDRKYHLKTYPNCFTGSELVDWLLDNLE-AITREEAVELGQALLKHGLIHHVsDDKHTFRDSYAL 79


                ..
gi 32171383 114 YQ 115
Cdd:cd04371  80 YR 81
DEP pfam00610
Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for ...
 49-116 3.58e-14

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit.


Pssm-ID: 459867  Cd Length: 71  Bit Score: 68.00  E-value: 3.58e-14
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 32171383    49 VKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSV-DQHLYFQDNYVFYQF 116
Cdd:pfam00610   4 LKDRRKHLKTYPNCFTGSEAVDWLMDNLE-IITREEAVELGQLLLDQGLIHHVgDKHGLFKDSYYFYRF 71
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 304-391 2.07e-11

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 61.17  E-value: 2.07e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383   304 VVVSGTPEKILEHLLNDLHLAEvqhketETLLDDFLLTYTVFMTTDDLCQALLRHY----SAKKYQGEEENSDV--PCRK 377
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRIMLD------DSFLSTFLLTYRSFTTPAELLELLIERYnippPLDLSSDSYWISKKtlPIRI 74

                          90
                  ....*....|....
gi 32171383   378 RkVLHLVSQWISLY 391
Cdd:pfam00618  75 R-VLSVLRHWVENY 87
DEP smart00049
Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in ...
 49-118 1.38e-10

Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in signalling proteins that contain PH, rasGEF, rhoGEF, rhoGAP, RGS, PDZ domains. DEP domain in Drosophila dishevelled is essential to rescue planar polarity defects and induce JNK signalling (Cell 94, 109-118).


Pssm-ID: 214489  Cd Length: 77  Bit Score: 58.06  E-value: 1.38e-10
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 32171383     49 VKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSVDQH--LYFQDNYVFYQFSS 118
Cdd:smart00049   7 LRDRKYFLKTYPNCFTGSELVDWLMDNLE-IIDREEAVHLGQLLLDEGLIHHVNGPnkHTFKDSKALYRFTT 77
DEP_1_DEP6 cd04442
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in DEP6-like proteins. DEP6 proteins ...
 33-116 2.05e-08

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in DEP6-like proteins. DEP6 proteins contain two DEP and a PDZ domain. Their function is unknown.


Pssm-ID: 239889 [Multi-domain]  Cd Length: 82  Bit Score: 51.82  E-value: 2.05e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 32171383  33 AGRALRniIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQcRSMAIGVWQLLLDMGIMSSV-DQHLYFQDNY 111
Cdd:cd04442   1 TGEQLR--LRLHEAKVIKDRRHHLRTYPNCFVGKELIDWLIEHKEASD-RETAIKIMQKLLDHSIIHHVcDEHKEFKDAK 77


                ....*
gi 32171383 112 VFYQF 116
Cdd:cd04442  78 LFYRF 82
DEP_2_DEP6 cd04441
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in DEP6-like proteins. DEP6 proteins ...
 59-116 7.83e-06

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in DEP6-like proteins. DEP6 proteins contain two DEP and a PDZ domain. Their function is unknown.


Pssm-ID: 239888  Cd Length: 85  Bit Score: 44.73  E-value: 7.83e-06
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 32171383  59 YRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQF 116
Cdd:cd04441  29 YERTFVGSEFIDWLLQEGE-AESRREAVQLCRRLLEHGIIQHVSNKHHFFDSNLLYQF 85
DEP_GPR155 cd04443
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in GPR155-like proteins. GRP155-like ...
 46-116 1.48e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in GPR155-like proteins. GRP155-like proteins, also known as PGR22, contain an N-terminal permease domain, a central transmembrane region and a C-terminal DEP domain. They are orphan receptors of the class B G protein-coupled receptors. Their function is unknown.


Pssm-ID: 239890 [Multi-domain]  Cd Length: 83  Bit Score: 43.86  E-value: 1.48e-05
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 32171383  46 ADLVKDRVNLKGFYRRSCVGSELVDWLLEhCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQF 116
Cdd:cd04443  14 QDIVKDRRCGLRTYKGVFCGCDLVSWLIE-VGLAQDRGEAVLYGRRLLQGGVLQHITNEHHFRDENLLYRF 83
DEP_DEPDC5-like cd04449
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC5-like proteins. DEPDC5, in ...
 48-116 5.49e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC5-like proteins. DEPDC5, in human also known as KIAA0645, is a DEP domain containing protein of unknown function.


Pssm-ID: 239896  Cd Length: 83  Bit Score: 42.26  E-value: 5.49e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 32171383  48 LVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQF 116
Cdd:cd04449  15 GIFDRSWHKGLPSNCFIGSEAVSWLINNFEDVDTREEAVELGQELMNEGLIEHVSGRHPFLDGFYFYYI 83
DEP_PIKfyve cd04448
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in fungal RhoGEF (GDP/GTP exchange ...
 59-115 8.13e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in fungal RhoGEF (GDP/GTP exchange factor) PIKfyve-like proteins. PIKfyve contains N-terminal Fyve finger and DEP domains, a central chaperonin-like domain and a C-terminal PIPK (phosphatidylinositol phosphate kinase) domain. PIKfyve-like proteins are important phosphatidylinositol (3)-monophosphate (PtdIns(3)P)-5-kinases, producing PtdIns(3,5)P2, which plays a major role in multivesicular body (MVB) sorting and control of retrograde traffic from the vacuole back to the endosome and/or Golgi. PIKfyve itself has been shown to be play a role in regulating early-endosome-to-trans-Golgi network (TGN) retrograde trafficking.


Pssm-ID: 239895  Cd Length: 81  Bit Score: 41.66  E-value: 8.13e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 32171383  59 YRRSCVGSELVDWLLEHCPFVQcRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQ 115
Cdd:cd04448  25 YTNCILGKELVNWLIRQGKAAT-RVQAIAIGQALLDAGWIECVSDDDLFRDEYALYK 80
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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