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Conserved domains on  [gi|389740405|gb|EIM81596|]
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hypothetical protein STEHIDRAFT_171898 [Stereum hirsutum FP-91666 SS1]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
POLO_box super family cl02916
Polo-box domain (PBD), a C-terminal tandemly repeated region of polo-like kinases; The ...
 197-303 3.68e-35

Polo-box domain (PBD), a C-terminal tandemly repeated region of polo-like kinases; The polo-like Ser/Thr kinases (Plk1, Plk2/Snk, Plk3/Prk/Fnk, Plk4/Sak, and the inactive kinase Plk5) play various roles in cytokinesis and mitosis. At their C-terminus, they contain a tandemly repeated polo-box domain (in the case of Plk4, a tandem repeat of cryptic PBDs is found in the middle of the protein followed by a C-terminal single repeat), which appears to be involved in autoinhibition and in mediating the subcellular localization. The latter may be controlled via interactions between the polo-box domain and phospho-peptide motifs. The phosphopeptide binding site is formed at the interface between the two tandemly repeated PBDs. The PBDs of Plk4/Sak appear unique in participating in homodimer interactions, though it is not clear whether and how they interact with phosphopeptides.


The actual alignment was detected with superfamily member cd13114:

Pssm-ID: 470700  Cd Length: 112  Bit Score: 128.92  E-value: 3.68e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 389740405 197 SSSAPTSLTTNLLRSKTHKVTQGQVVVLPSKSLLVDFREGERRkGRQGNEVLVISPDGEQIQVFSAPHLST------PCC 270
Cdd:cd13114    1 LRSRVPPLNTSRLRPIRQKTKNAVVSILEDGEVCLEFLKGKGR-EERVVEVLRISSDGQKIVIYQPNGGKGvpleppPLP 79

                         90       100       110
                 ....*....|....*....|....*....|...
gi 389740405 271 LAEASATYSLEDLPEPYLKVYQQAARVIEHVKK 303
Cdd:cd13114   80 PPGADATYSYESLPEKYWKKYQYAARFVQLVKS 112
 
Name Accession Description Interval E-value
POLO_box_Plk4_1 cd13114
First (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak); The polo-like Ser/Thr ...
 197-303 3.68e-35

First (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak); The polo-like Ser/Thr kinases (Plk1, Plk2/Snk, Plk3/Prk/Fnk, Plk4/Sak, and the inactive kinase Plk5) play various roles in cytokinesis and mitosis. At their C-terminus, they contain a tandemly repeated polo-box domain (in the case of Plk4, a tandem repeat of cryptic PBDs is found in the middle of the protein followed by a C-terminal single repeat), which appears to be involved in autoinhibition and in mediating the subcellular localization. The latter may be controlled via interactions between the polo-box domain and phospho-peptide motifs. The phosphopeptide binding site is formed at the interface between the two tandemly repeated PBDs. The PBDs of Plk4/Sak appear unique in participating in homodimer interactions, though it is not clear whether and how they interact with phosphopeptides.


Pssm-ID: 240557  Cd Length: 112  Bit Score: 128.92  E-value: 3.68e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 389740405 197 SSSAPTSLTTNLLRSKTHKVTQGQVVVLPSKSLLVDFREGERRkGRQGNEVLVISPDGEQIQVFSAPHLST------PCC 270
Cdd:cd13114    1 LRSRVPPLNTSRLRPIRQKTKNAVVSILEDGEVCLEFLKGKGR-EERVVEVLRISSDGQKIVIYQPNGGKGvpleppPLP 79

                         90       100       110
                 ....*....|....*....|....*....|...
gi 389740405 271 LAEASATYSLEDLPEPYLKVYQQAARVIEHVKK 303
Cdd:cd13114   80 PPGADATYSYESLPEKYWKKYQYAARFVQLVKS 112
Plk4_PB1 pfam18190
Polo-like Kinase 4 Polo Box 1; This domain is found in Polo-like kinase 4 (Plk4) present in ...
 203-302 4.16e-06

Polo-like Kinase 4 Polo Box 1; This domain is found in Polo-like kinase 4 (Plk4) present in Drosophila melanogaster. Plk4 is a conserved component in the duplication pathway of centrioles which is needed to prevent chromosomal instability. The domain is Polo Box 1 (PB1) and has a pseudo-symmetric dimerization interface across PB1-PB1.


Pssm-ID: 465672  Cd Length: 107  Bit Score: 46.20  E-value: 4.16e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 389740405  203 SLTTNLLRSKTHKVTQGQVVVLPSKSLLVDF---REGERRKgrqgNEVLVISPDGEQIQVFSaPHLSTPCCLAEASA--- 276
Cdd:pfam18190   3 PLNSYRLRPIRQKTKNAVVSILESGEVCLEFvkqKNGEEYV----VEVLRISQDGQEITIYQ-PNRGRGFPLNDRPPepp 77

                          90       100       110
                  ....*....|....*....|....*....|
gi 389740405  277 ----TYSLEDLPEPYLKVYQQAARVIEHVK 302
Cdd:pfam18190  78 gsdkVYSLDNLPEKYWKKYQYASRFVNLVR 107
 
Name Accession Description Interval E-value
POLO_box_Plk4_1 cd13114
First (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak); The polo-like Ser/Thr ...
 197-303 3.68e-35

First (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak); The polo-like Ser/Thr kinases (Plk1, Plk2/Snk, Plk3/Prk/Fnk, Plk4/Sak, and the inactive kinase Plk5) play various roles in cytokinesis and mitosis. At their C-terminus, they contain a tandemly repeated polo-box domain (in the case of Plk4, a tandem repeat of cryptic PBDs is found in the middle of the protein followed by a C-terminal single repeat), which appears to be involved in autoinhibition and in mediating the subcellular localization. The latter may be controlled via interactions between the polo-box domain and phospho-peptide motifs. The phosphopeptide binding site is formed at the interface between the two tandemly repeated PBDs. The PBDs of Plk4/Sak appear unique in participating in homodimer interactions, though it is not clear whether and how they interact with phosphopeptides.


Pssm-ID: 240557  Cd Length: 112  Bit Score: 128.92  E-value: 3.68e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 389740405 197 SSSAPTSLTTNLLRSKTHKVTQGQVVVLPSKSLLVDFREGERRkGRQGNEVLVISPDGEQIQVFSAPHLST------PCC 270
Cdd:cd13114    1 LRSRVPPLNTSRLRPIRQKTKNAVVSILEDGEVCLEFLKGKGR-EERVVEVLRISSDGQKIVIYQPNGGKGvpleppPLP 79

                         90       100       110
                 ....*....|....*....|....*....|...
gi 389740405 271 LAEASATYSLEDLPEPYLKVYQQAARVIEHVKK 303
Cdd:cd13114   80 PPGADATYSYESLPEKYWKKYQYAARFVQLVKS 112
Plk4_PB1 pfam18190
Polo-like Kinase 4 Polo Box 1; This domain is found in Polo-like kinase 4 (Plk4) present in ...
 203-302 4.16e-06

Polo-like Kinase 4 Polo Box 1; This domain is found in Polo-like kinase 4 (Plk4) present in Drosophila melanogaster. Plk4 is a conserved component in the duplication pathway of centrioles which is needed to prevent chromosomal instability. The domain is Polo Box 1 (PB1) and has a pseudo-symmetric dimerization interface across PB1-PB1.


Pssm-ID: 465672  Cd Length: 107  Bit Score: 46.20  E-value: 4.16e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 389740405  203 SLTTNLLRSKTHKVTQGQVVVLPSKSLLVDF---REGERRKgrqgNEVLVISPDGEQIQVFSaPHLSTPCCLAEASA--- 276
Cdd:pfam18190   3 PLNSYRLRPIRQKTKNAVVSILESGEVCLEFvkqKNGEEYV----VEVLRISQDGQEITIYQ-PNRGRGFPLNDRPPepp 77

                          90       100       110
                  ....*....|....*....|....*....|
gi 389740405  277 ----TYSLEDLPEPYLKVYQQAARVIEHVK 302
Cdd:pfam18190  78 gsdkVYSLDNLPEKYWKKYQYASRFVNLVR 107
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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