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Conserved domains on  [gi|500218792|ref|WP_011888932|]
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phage head-tail connector protein [Streptococcus pyogenes]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
gp6_gp15_like super family cl12049
Head-Tail Connector Proteins gp6 and gp15, and similar proteins; Members of this family ...
 7-87 2.53e-07

Head-Tail Connector Proteins gp6 and gp15, and similar proteins; Members of this family include the proteins gp6 and gp15 from bacteriophage HK97 and SPP1, respectively. They are critical in the assembly of the connector, a specialized structure that serves as an interface for head and tail attachment, as well as a point at which DNA exits the head during infection by the bacteriophage. They form dodecameric ring structures that comprise the middle ring of the connector, located between the portal protein (attached to the head) and the gp7/gp16 ring (attached to the tail). They are components of the mature phage and the absence or mutation of HK97 gp6 or SPP1 gp15, respectively, result in defective head-tail joining and the absence of mature phage particles. The genome maps of HK97 and SPP1 show that genes encoding gp6 and gp15 are in the same relative position on the genome, located adjacent to the major capsid protein (MCP) gene and in between head and tail genes. Also included in this family is the uncharacterized Bacillus subtilis Yqbg protein, whose gene is part of the unusual genetic element called skin. The Yqbg gene is surrounded with genes similar to genes in the Bacillus subtilis prophage-like element PBSX, which encode for proteins comprising contractile-tailed phage-like particles that are produced upon mitomycin C treatment. Yqbg likely acts as a head-tail connector protein, similar to gp6 and gp15, of the PBSX-like prophage encoded in the skin element.


The actual alignment was detected with superfamily member pfam05135:

Pssm-ID: 448390  Cd Length: 93  Bit Score: 45.09  E-value: 2.53e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 500218792    7 IDDIKLFIGIskGDGAQDEIITLAIYESKERVLAKLNEY-SETEITKIPDRLRFIVRDVAIKRFNRINSEGAVEDSEEGK 85
Cdd:pfam05135   4 LEDIKTLLGI--DDDSDDALLNSYIDAAEEYIENYLGRSlGDPDAISLPADLKYAVLELAVARYNNRGNEGMSSYSEEGV 81


                  ..
gi 500218792   86 TF 87
Cdd:pfam05135  82 SL 83
 
Name Accession Description Interval E-value
Phage_connect_1 pfam05135
Phage gp6-like head-tail connector protein; This family of proteins contain head-tail ...
 7-87 2.53e-07

Phage gp6-like head-tail connector protein; This family of proteins contain head-tail connector proteins related to gp6 from bacteriophage HK97. A structure of this protein shows similarity to gp15 a well characterized connector component of bacteriophage SPP1.


Pssm-ID: 428326  Cd Length: 93  Bit Score: 45.09  E-value: 2.53e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 500218792    7 IDDIKLFIGIskGDGAQDEIITLAIYESKERVLAKLNEY-SETEITKIPDRLRFIVRDVAIKRFNRINSEGAVEDSEEGK 85
Cdd:pfam05135   4 LEDIKTLLGI--DDDSDDALLNSYIDAAEEYIENYLGRSlGDPDAISLPADLKYAVLELAVARYNNRGNEGMSSYSEEGV 81


                  ..
gi 500218792   86 TF 87
Cdd:pfam05135  82 SL 83
gp15 cd08055
Head-Tail Connector Protein gp15 of Bacteriophage SPP1 and similar proteins; The bacteriophage ...
 8-102 2.32e-04

Head-Tail Connector Protein gp15 of Bacteriophage SPP1 and similar proteins; The bacteriophage SPP1 gp15 protein is critical in the assembly of the connector, a specialized structure that serves as an interface for head and tail attachment, as well as a point at which DNA exits the head during infection by the bacteriophage. It forms a dodecameric ring structure that comprises the middle ring of the connector, located between the portal protein (attached to the head) and the gp16 ring (attached to the tail). Binding of the gp15 and gp16 rings to the portal protein is essential to prevent leakage of packaged DNA. gp15 is a component of the mature phage and its mutation results in defective head-tail joining.


Pssm-ID: 153447  Cd Length: 95  Bit Score: 37.30  E-value: 2.32e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 500218792   8 DDIKLFIGISKGDGAQDEIITLAIYESKERVLAklneysETEITKIPDRLRFIVRDVAIKRFNRINSEGAVEDSEEGK-- 85
Cdd:cd08055    1 EKVKKLLGISLDDNSDDELLEELIEDAIEDVLD------YCNQTFLPDPLLPTVVKLAVALYNKLGTEGEISSRSEGDvs 74

                         90
                 ....*....|....*...
gi 500218792  86 -TFKwDSYLKEYESTLRS 102
Cdd:cd08055   75 vSYE-TGLPKSILKGLNP 91
 
Name Accession Description Interval E-value
Phage_connect_1 pfam05135
Phage gp6-like head-tail connector protein; This family of proteins contain head-tail ...
 7-87 2.53e-07

Phage gp6-like head-tail connector protein; This family of proteins contain head-tail connector proteins related to gp6 from bacteriophage HK97. A structure of this protein shows similarity to gp15 a well characterized connector component of bacteriophage SPP1.


Pssm-ID: 428326  Cd Length: 93  Bit Score: 45.09  E-value: 2.53e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 500218792    7 IDDIKLFIGIskGDGAQDEIITLAIYESKERVLAKLNEY-SETEITKIPDRLRFIVRDVAIKRFNRINSEGAVEDSEEGK 85
Cdd:pfam05135   4 LEDIKTLLGI--DDDSDDALLNSYIDAAEEYIENYLGRSlGDPDAISLPADLKYAVLELAVARYNNRGNEGMSSYSEEGV 81


                  ..
gi 500218792   86 TF 87
Cdd:pfam05135  82 SL 83
gp15 cd08055
Head-Tail Connector Protein gp15 of Bacteriophage SPP1 and similar proteins; The bacteriophage ...
 8-102 2.32e-04

Head-Tail Connector Protein gp15 of Bacteriophage SPP1 and similar proteins; The bacteriophage SPP1 gp15 protein is critical in the assembly of the connector, a specialized structure that serves as an interface for head and tail attachment, as well as a point at which DNA exits the head during infection by the bacteriophage. It forms a dodecameric ring structure that comprises the middle ring of the connector, located between the portal protein (attached to the head) and the gp16 ring (attached to the tail). Binding of the gp15 and gp16 rings to the portal protein is essential to prevent leakage of packaged DNA. gp15 is a component of the mature phage and its mutation results in defective head-tail joining.


Pssm-ID: 153447  Cd Length: 95  Bit Score: 37.30  E-value: 2.32e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 500218792   8 DDIKLFIGISKGDGAQDEIITLAIYESKERVLAklneysETEITKIPDRLRFIVRDVAIKRFNRINSEGAVEDSEEGK-- 85
Cdd:cd08055    1 EKVKKLLGISLDDNSDDELLEELIEDAIEDVLD------YCNQTFLPDPLLPTVVKLAVALYNKLGTEGEISSRSEGDvs 74

                         90
                 ....*....|....*...
gi 500218792  86 -TFKwDSYLKEYESTLRS 102
Cdd:cd08055   75 vSYE-TGLPKSILKGLNP 91
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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