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Conserved domains on  [gi|576290961|gb|AHH29451|]
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neomycin phosphotransferase III, partial [Enterococcus faecium]

Protein Classification

aminoglycoside 3'-phosphotransferase( domain architecture ID 10790026)

aminoglycoside 3'-phosphotransferase phosphorylates and inactives antibiotic substrates such as kanamycin, streptomycin, neomycin, and gentamicin, among others

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
1-250 3.29e-106

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 307.61  E-value: 3.29e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   1 LIE*YRCVKDTEGMSPAKVYKLV-GENENLYLKMTdsrYKGTTYDVEREKDMMLWLEGK-LPVPKVLHFERHDGWSNLLM 78
Cdd:COG3231   11 LLGGYRWEPVTIGESGAKVFRLAdGGRPTLYLKIE---PAGPAAELEDEADRLRWLAGQgLPVPEVLDFGEDDGGAWLLT 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  79 SEADGVLCSEEYEdEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLadVDCENWEEDTPFKDPRE 158
Cdd:COG3231   88 TAVPGRPAASVSE-ALDPERAVELLAEALRRLHALPVADCPFDRRLERRLAEARARVAAGL--VDPDDFDEERRGRPPEE 164

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 159 LYDFLKTEKP-EEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEqYVELFFDLLGIKP 237
Cdd:COG3231  165 LLAELLAERPaEEDLVVTHGDACLPNILVDPGTFSGFIDLGRLGVADRYQDLALAARSLRENLGEG-WVEPFLDAYGIAP 243

                        250
                 ....*....|...
gi 576290961 238 DWEKIKYYILLDE 250
Cdd:COG3231  244 DPERLAFYRLLDE 256
 
Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
1-250 3.29e-106

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 307.61  E-value: 3.29e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   1 LIE*YRCVKDTEGMSPAKVYKLV-GENENLYLKMTdsrYKGTTYDVEREKDMMLWLEGK-LPVPKVLHFERHDGWSNLLM 78
Cdd:COG3231   11 LLGGYRWEPVTIGESGAKVFRLAdGGRPTLYLKIE---PAGPAAELEDEADRLRWLAGQgLPVPEVLDFGEDDGGAWLLT 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  79 SEADGVLCSEEYEdEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLadVDCENWEEDTPFKDPRE 158
Cdd:COG3231   88 TAVPGRPAASVSE-ALDPERAVELLAEALRRLHALPVADCPFDRRLERRLAEARARVAAGL--VDPDDFDEERRGRPPEE 164

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 159 LYDFLKTEKP-EEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEqYVELFFDLLGIKP 237
Cdd:COG3231  165 LLAELLAERPaEEDLVVTHGDACLPNILVDPGTFSGFIDLGRLGVADRYQDLALAARSLRENLGEG-WVEPFLDAYGIAP 243

                        250
                 ....*....|...
gi 576290961 238 DWEKIKYYILLDE 250
Cdd:COG3231  244 DPERLAFYRLLDE 256
APH cd05150
Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group ...
 5-250 1.65e-100

Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group from ATP to aminoglycoside antibiotics such as kanamycin, streptomycin, neomycin, and gentamicin, among others. The aminoglycoside antibiotics target the 30S ribosome and promote miscoding, leading to the production of defective proteins which insert into the bacterial membrane, resulting in membrane damage and the ultimate demise of the bacterium. Phosphorylation of the aminoglycoside antibiotics results in their inactivation, leading to bacterial antibiotic resistance. The APH gene is found on transposons and plasmids and is thought to have originated as a self-defense mechanism used by microorganisms that produce the antibiotics. The APH subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270699 [Multi-domain]  Cd Length: 244  Bit Score: 292.56  E-value: 1.65e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   5 YRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYkgtTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGV 84
Cdd:cd05150    1 YRWEPDTIGESGARVYRLDGGGPVLYLKTAPAGY---AYELAREAERLRWLAGKLPVPEVLDYGSDDGGDWLLTTALPGR 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  85 LCSEEYEDEQsPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDceNWEEDTPFKDPRELYDFLK 164
Cdd:cd05150   78 DAASLEPLLD-PERLVDLLAEALRALHSLPIADCPFDRRLDARLAEARARVEAGLVDED--DFDEERQGRTAEELLAELE 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 165 TEKPEEE-LVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI-KPDWEKI 242
Cdd:cd05150  155 ATRPAEEdLVVTHGDACLPNIILDPGRFSGFIDLGRLGVADRYQDLALAVRSLRENLGGEEYAERFLDAYGIdAPDPERL 234


                 ....*...
gi 576290961 243 KYYILLDE 250
Cdd:cd05150  235 AYYRLLDE 242
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 19-245 9.96e-25

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 98.34  E-value: 9.96e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   19 VYKLVGENENLYLKmtDSRYKGTTYDVEREKDMMLWL--EGKLPVPKVLHFER---HDGWSNLLMSEADGVLCsEEYEDE 93
Cdd:pfam01636  13 TYLVTTGDGRYVLR--LPPPGRAAEELRRELALLRHLaaAGVPPVPRVLAGCTdaeLLGLPFLLMEYLPGEVL-ARPLLP 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   94 QSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENweEDTPFKDPRELYDFLKTEKP-EEEL 172
Cdd:pfam01636  90 EERGALLEALGRALARLHAVDPAALPLAGRLARLLELLRQLEAALARLLAAEL--LDRLEELEERLLAALLALLPaELPP 167

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 576290961  173 VFSHGDLGDSNIFV-KDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLgiKPDWEKIKYY 245
Cdd:pfam01636 168 VLVHGDLHPGNLLVdPGGRVSGVIDFEDAGLGDPAYDLAILLNSWGRELGAELLAAYLAAYG--AFGYARLREL 239
 
Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
1-250 3.29e-106

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 307.61  E-value: 3.29e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   1 LIE*YRCVKDTEGMSPAKVYKLV-GENENLYLKMTdsrYKGTTYDVEREKDMMLWLEGK-LPVPKVLHFERHDGWSNLLM 78
Cdd:COG3231   11 LLGGYRWEPVTIGESGAKVFRLAdGGRPTLYLKIE---PAGPAAELEDEADRLRWLAGQgLPVPEVLDFGEDDGGAWLLT 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  79 SEADGVLCSEEYEdEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLadVDCENWEEDTPFKDPRE 158
Cdd:COG3231   88 TAVPGRPAASVSE-ALDPERAVELLAEALRRLHALPVADCPFDRRLERRLAEARARVAAGL--VDPDDFDEERRGRPPEE 164

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 159 LYDFLKTEKP-EEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEqYVELFFDLLGIKP 237
Cdd:COG3231  165 LLAELLAERPaEEDLVVTHGDACLPNILVDPGTFSGFIDLGRLGVADRYQDLALAARSLRENLGEG-WVEPFLDAYGIAP 243

                        250
                 ....*....|...
gi 576290961 238 DWEKIKYYILLDE 250
Cdd:COG3231  244 DPERLAFYRLLDE 256
APH cd05150
Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group ...
 5-250 1.65e-100

Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group from ATP to aminoglycoside antibiotics such as kanamycin, streptomycin, neomycin, and gentamicin, among others. The aminoglycoside antibiotics target the 30S ribosome and promote miscoding, leading to the production of defective proteins which insert into the bacterial membrane, resulting in membrane damage and the ultimate demise of the bacterium. Phosphorylation of the aminoglycoside antibiotics results in their inactivation, leading to bacterial antibiotic resistance. The APH gene is found on transposons and plasmids and is thought to have originated as a self-defense mechanism used by microorganisms that produce the antibiotics. The APH subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270699 [Multi-domain]  Cd Length: 244  Bit Score: 292.56  E-value: 1.65e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   5 YRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYkgtTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGV 84
Cdd:cd05150    1 YRWEPDTIGESGARVYRLDGGGPVLYLKTAPAGY---AYELAREAERLRWLAGKLPVPEVLDYGSDDGGDWLLTTALPGR 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  85 LCSEEYEDEQsPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDceNWEEDTPFKDPRELYDFLK 164
Cdd:cd05150   78 DAASLEPLLD-PERLVDLLAEALRALHSLPIADCPFDRRLDARLAEARARVEAGLVDED--DFDEERQGRTAEELLAELE 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 165 TEKPEEE-LVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI-KPDWEKI 242
Cdd:cd05150  155 ATRPAEEdLVVTHGDACLPNIILDPGRFSGFIDLGRLGVADRYQDLALAVRSLRENLGGEEYAERFLDAYGIdAPDPERL 234


                 ....*...
gi 576290961 243 KYYILLDE 250
Cdd:cd05150  235 AYYRLLDE 242
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 5-218 1.10e-30

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 111.63  E-value: 1.10e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   5 YRCVKDTEGMSpAKVYkLVGENENLYLKMTDSRYKgttYDVEREKDMMLWLEGK--LPVPKVLHFERHDGWSNLLMSEAD 82
Cdd:cd05120    1 ISVKLIKEGGD-NKVY-LLGDPREYVLKIGPPRLK---KDLEKEAAMLQLLAGKlsLPVPKVYGFGESDGWEYLLMERIE 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  83 GVLCSEEY--EDEQSPEKIIELYAECIRLFHSIDISdcpytnsldsrlaeldyllnndladvdcenweedtpfkdprely 160
Cdd:cd05120   76 GETLSEVWprLSEEEKEKIADQLAEILAALHRIDSS-------------------------------------------- 111

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 576290961 161 dflktekpeeelVFSHGDLGDSNIFVKD-GKVSGFIDLGRSGRADKWYDIAFCVRSIRE 218
Cdd:cd05120  112 ------------VLTHGDLHPGNILVKPdGKLSGIIDWEFAGYGPPAFDYAAALRDWTE 158
PKc_like cd13968
Catalytic domain of the Protein Kinase superfamily; The PK superfamily contains the large ...
 10-198 1.50e-29

Catalytic domain of the Protein Kinase superfamily; The PK superfamily contains the large family of typical PKs that includes serine/threonine kinases (STKs), protein tyrosine kinases (PTKs), and dual-specificity PKs that phosphorylate both serine/threonine and tyrosine residues of target proteins, as well as pseudokinases that lack crucial residues for catalytic activity and/or ATP binding. It also includes phosphoinositide 3-kinases (PI3Ks), aminoglycoside 3'-phosphotransferases (APHs), choline kinase (ChoK), Actin-Fragmin Kinase (AFK), and the atypical RIO and Abc1p-like protein kinases. These proteins catalyze the transfer of the gamma-phosphoryl group from ATP to their target substrates; these include serine/threonine/tyrosine residues in proteins for typical or atypical PKs, the 3-hydroxyl of the inositol ring of D-myo-phosphatidylinositol (PtdIns) or its derivatives for PI3Ks, the 4-hydroxyl of PtdIns for PI4Ks, and other small molecule substrates for APH/ChoK and similar proteins such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine.


Pssm-ID: 270870 [Multi-domain]  Cd Length: 136  Bit Score: 107.91  E-value: 1.50e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  10 DTEGMsPAKVYKLVGE--NENLYLKMTDSRYKGTTYDVEREKDMMLWLEGK-LPVPKVLHFERHDGWSNLLMSEADGVLC 86
Cdd:cd13968    1 MGEGA-SAKVFWAEGEctTIGVAVKIGDDVNNEEGEDLESEMDILRRLKGLeLNIPKVLVTEDVDGPNILLMELVKGGTL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  87 SE----EYEDEQSPEKIIELYAECIRLFHSIdisdcpytnsldsrlaeldyllnndladvdcenweedtpfkdprelydf 162
Cdd:cd13968   80 IAytqeEELDEKDVESIMYQLAECMRLLHSF------------------------------------------------- 110

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 576290961 163 lktekpeeelVFSHGDLGDSNIFVKDGKVSGFIDLG 198
Cdd:cd13968  111 ----------HLIHRDLNNDNILLSEDGNVKLIDFG 136
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 19-245 9.96e-25

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 98.34  E-value: 9.96e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   19 VYKLVGENENLYLKmtDSRYKGTTYDVEREKDMMLWL--EGKLPVPKVLHFER---HDGWSNLLMSEADGVLCsEEYEDE 93
Cdd:pfam01636  13 TYLVTTGDGRYVLR--LPPPGRAAEELRRELALLRHLaaAGVPPVPRVLAGCTdaeLLGLPFLLMEYLPGEVL-ARPLLP 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961   94 QSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENweEDTPFKDPRELYDFLKTEKP-EEEL 172
Cdd:pfam01636  90 EERGALLEALGRALARLHAVDPAALPLAGRLARLLELLRQLEAALARLLAAEL--LDRLEELEERLLAALLALLPaELPP 167

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 576290961  173 VFSHGDLGDSNIFV-KDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLgiKPDWEKIKYY 245
Cdd:pfam01636 168 VLVHGDLHPGNLLVdPGGRVSGVIDFEDAGLGDPAYDLAILLNSWGRELGAELLAAYLAAYG--AFGYARLREL 239
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 22-250 1.05e-12

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 66.29  E-value: 1.05e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  22 LVGENENLYLKMtDSRYKGTTYDVEREKDMMLWLEGKL--PVPKVLHFERHD---GWSNLLMSEADGVlCSEEYEDEQSP 96
Cdd:COG3173   38 RLDTGDRLVLRR-PPRGLASAHDVRREARVLRALAPRLgvPVPRPLALGEDGeviGAPFYVMEWVEGE-TLEDALPDLSP 115

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  97 EKIIELYAECIRLF---HSIDISDCPYTnslDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEE-EL 172
Cdd:COG3173  116 AERRALARALGEFLaalHAVDPAAAGLA---DGRPEGLERQLARWRAQLRRALARTDDLPALRERLAAWLAANLPEWgPP 192

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 173 VFSHGDLGDSNIFV--KDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIG----EEQYVELFFDLLGIKPDWEkikYYI 246
Cdd:COG3173  193 VLVHGDLRPGNLLVdpDDGRLTAVIDWELATLGDPAADLAYLLLYWRLPDDllgpRAAFLAAYEEATGDLDDLT---WWA 269


                 ....
gi 576290961 247 LLDE 250
Cdd:COG3173  270 LADP 273
SrkA COG2334
Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal ...
 176-231 1.41e-05

Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal transduction mechanisms]; Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist is part of the Pathway/BioSystem: Threonine biosynthesis


Pssm-ID: 441905 [Multi-domain]  Cd Length: 297  Bit Score: 45.30  E-value: 1.41e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 576290961 176 HGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFD 231
Cdd:COG2334  183 HGDLHPDNVLFDGDGVSGLIDFDDAGYGPRLYDLAIALNGWADGPLDPARLAALLE 238
ACAD10_11_N-like cd05154
N-terminal domain of Acyl-CoA dehydrogenase (ACAD) 10 and 11, and similar proteins; This ...
 41-235 5.23e-05

N-terminal domain of Acyl-CoA dehydrogenase (ACAD) 10 and 11, and similar proteins; This subfamily is composed of the N-terminal domains of vertebrate ACAD10 and ACAD11, and similar uncharacterized bacterial and eukaryotic proteins. ACADs are a family of flavoproteins that are involved in the beta-oxidation of fatty acyl-CoA derivatives. ACAD deficiency can cause metabolic disorders including muscle fatigue, hypoglycemia, and hepatic lipidosis. There are at least 11 distinct ACADs, some of which show distinct substrate specificities to either straight-chain or branched-chain fatty acids. ACAD10 is widely expressed in human tissues and highly expressed in liver, kidney, pancreas, and spleen. ACAD10 and ACAD11 are both significantly expressed in human brain tissues. They contain a long N-terminal domain with similarity to phosphotransferases with a Protein Kinase fold, which is absent in other ACADs. They may exhibit multiple functions in acyl-CoA oxidation pathways. ACAD11 utilizes substrates with carbon chain lengths of 20 to 26, with optimal activity towards C22CoA. ACAD10 may be associated with an increased risk in type II diabetes. The ACAD10/11-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270703 [Multi-domain]  Cd Length: 254  Bit Score: 43.37  E-value: 5.23e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  41 TTYDVEREKDMMLWLEGK-LPVPKVLHFE---RHDGWSNLLMSEADG-VLCSEEYEDEQSPEKIIELYAECIRLF---HS 112
Cdd:cd05154   41 SAHDLEREYRVLRALAGTgVPVPRVLALCedpSVLGAPFYVMERVDGrVLPDPLPRPDLSPEERRALARSLVDALaalHS 120

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 113 IDISDCP---YTNSLDSRLAELDYLLnndladvdcENWEEDTPFKDP--RELYDFLKTEKPEE-ELVFSHGDLGDSN-IF 185
Cdd:cd05154  121 VDPAALGladLGRPEGYLERQVDRWR---------RQLEAAATDPPPalEEALRWLRANLPADgRPVLVHGDFRLGNlLF 191

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 576290961 186 VKDGKVSGFIDLGRSGRADKWYDIA-FCVRSIREdiGEEQYVELFFDLLGI 235
Cdd:cd05154  192 DPDGRVTAVLDWELATLGDPLEDLAwLLARWWRP--GDPPGLAAPTRLPGF 240
HomoserineK_II cd05153
Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a ...
 176-231 1.18e-04

Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a subset of bacteria, which have a Protein Kinase fold. These proteins do not bear any similarity to the GHMP family homoserine kinases present in most bacteria and eukaryotes. Homoserine kinase catalyzes the transfer of the gamma-phosphoryl group from ATP to L-homoserine producing L-homoserine phosphate, an intermediate in the production of the amino acids threonine, methionine, and isoleucine. The Type II homoserine kinase subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270702 [Multi-domain]  Cd Length: 300  Bit Score: 42.63  E-value: 1.18e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 576290961 176 HGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA-FCVRSIREDIG--EEQYVELFFD 231
Cdd:cd05153  183 HADLFRDNVLFDGDRLSGIIDFYDACYDPLLYDLAiALNDWCFDDDGklDPERAKALLA 241
CotS COG0510
Thiamine kinase or a related kinase [Coenzyme transport and metabolism];
157-248 6.08e-04

Thiamine kinase or a related kinase [Coenzyme transport and metabolism];


Pssm-ID: 440276 [Multi-domain]  Cd Length: 156  Bit Score: 39.38  E-value: 6.08e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 157 RELYDFLKT--EKPEEELVFSHGDLGDSNIFV-KDGKVSgFIDLGRSGRADKWYDIA-FCVRSIREDIGEEQYVELFFDL 232
Cdd:COG0510   32 LRRLEELERalAARPLPLVLCHGDLHPGNFLVtDDGRLY-LIDWEYAGLGDPAFDLAaLLVEYGLSPEQAEELLEAYGFG 110

                         90
                 ....*....|....*.
gi 576290961 233 LGIKPDWEKIKYYILL 248
Cdd:COG0510  111 RPTEELLRRLRAYRAL 126
Bud32 COG3642
tRNA A-37 threonylcarbamoyl transferase component Bud32 [Translation, ribosomal structure and ...
 45-210 2.64e-03

tRNA A-37 threonylcarbamoyl transferase component Bud32 [Translation, ribosomal structure and biogenesis]; tRNA A-37 threonylcarbamoyl transferase component Bud32 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 442859 [Multi-domain]  Cd Length: 159  Bit Score: 37.25  E-value: 2.64e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961  45 VEREKDMMLWLEGK-LPVPKVLHFERHDGWsnLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDIsdcpytns 123
Cdd:COG3642    3 TRREARLLRELREAgVPVPKVLDVDPDDAD--LVMEYIEGETLADLLEEGELPPELLRELGRLLARLHRAGI-------- 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 576290961 124 ldsrlaeldyllnndladvdcenweedtpfkdprelydflktekpeeelvfSHGDLGDSNIFVKDGKVsGFIDLGRSGRA 203
Cdd:COG3642   73 ---------------------------------------------------VHGDLTTSNILVDDGGV-YLIDFGLARYS 100


                 ....*..
gi 576290961 204 DKWYDIA 210
Cdd:COG3642  101 DPLEDKA 107
APH_ChoK_like_1 cd05155
Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and ...
 173-204 2.80e-03

Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and Choline kinase; This subfamily is composed of uncharacterized bacterial proteins with similarity to APH and ChoK. Other APH/ChoK-like proteins include ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). These proteins catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates, such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides, and macrolides leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270704 [Multi-domain]  Cd Length: 234  Bit Score: 37.99  E-value: 2.80e-03
                         10        20        30
                 ....*....|....*....|....*....|..
gi 576290961 173 VFSHGDLGDSNIFVKDGKVSGFIDLGRSGRAD 204
Cdd:cd05155  164 VWLHGDLHPGNLLVRDGRLSAVIDFGDLGVGD 195
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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