Aldo/keto reductase, related to diketogulonate reductase [Secondary metabolites biosynthesis, transport and catabolism];

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 6680019  147 WEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQV--NLASccvMPPDLTAFAKQFDIQL 211
Cdd:COG0656 110 WRALEELYEEGLIRAIGVSNFDPEHLEELLAETGVKPAVNQVelHPYL---QQRELLAFCREHGIVV 173

Saccharomyces pombe NAD/NADP-dependent indole-3-acetaldehyde reductase, Candida parapsilosis NADPH-dependent conjugated polyketone reductase C2 (CPR), and similar proteins; Saccharomyces pombe NAD/NADP-dependent indole-3-acetaldehyde reductase (EC 1.1.1.190/EC 1.1.1.191) and Candida parapsilosis NADPH-dependent CPR (EC 1.1.1.358/EC 1.1.1.168) are founding members of aldo-keto reductase family 3 member C2 (AKR3C2) and C3 (AKR3C3), respectively. Saccharomyces pombe NAD/NADP-dependent indole-3-acetaldehyde reductase catalyzes the conversion from (Indol-3-yl)ethanol to (indol-3-yl)acetaldehyde in a NAD/NADP-dependent manner. CPR, also called 2-dehydropantolactone reductase, or 2-dehydropantolactone reductase (A-specific), or ketopantoyl-lactone reductase, acts as a NADPH-dependent conjugated polyketone reductase with broad substrate specificity and strict stereospecificity. It reduces ketopantoyl lactone and isatin.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6680019  117 LGVAQLDSVIMASPPIEDGVNLSLEHLqpyWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVM 196
Cdd:cd19120  92 LGVDYVDLYLIHSPFFAKEGGPTLAEA---WAELEALKDAGLVRSIGVSNFRIEDLEELLDTAKIKPAVNQIEFHPYLYP 168

                        90       100       110
                ....*....|....*....|....*....|....
gi 6680019  197 P-PDLTAFAKQFDIQL--------LTHNDPKELL 221
Cdd:cd19120 169 QqPALLEYCREHGIVVsaysplspLTRDAGGPLD 202

AKR3E family of aldo-keto reductase (AKR); Trichoderma reesei NADP(+)-dependent glycerol 2-dehydrogenase (GLD2, EC 1.1.1.156), also called dihydroxyacetone reductase, is a founding member of aldo-keto reductase family 3 member E1 (AKR3E1). It acts as a glycerol oxidoreductase probably involved in glycerol synthesis.

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|
gi 6680019  141 EHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNL 190
Cdd:cd19122 139 ENPEPTWRAMEEIYESGKAKAIGVSNWTIPGLKKLLSFAKVKPHVNQIEI 188

AKR5A and AKR5G families of aldo-keto reductase (AKR); The AKR5A family of AKR includes prostaglandin F2-alpha synthase (PGFS) from Leishmania major (AKR5A1) and Trypanosoma brucei (AKR5A2). PGFS, also called 9,11-endoperoxide prostaglandin H2 reductase, catalyzes the NADP-dependent formation of prostaglandin F2-alpha from prostaglandin H2. It has also aldo/ketoreductase activity for synthetic substrates 9,10-phenanthrenequinone and p-nitrobenzaldehyde. The AKR5G family of AKR includes Bacillus subtilis glyoxal reductase (GR), uncharacterized oxidoreductase YtbE, and Bacillus aryabhattai aldo-keto reductase, which corresponds to aldo-keto reductase family 5 member G1-3 (AKR5G1-3), respectively. GR (YvgN, EC 1.1.1.283), also called methylglyoxal reductase, reduces glyoxal and methylglyoxal (2-oxopropanal). It is not involved in vitamin B6 biosynthesis.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 6680019  141 EHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLaSCCVMPPDLTAFAKQFDIQL 211
Cdd:cd19126 109 DKFIDTWKALEKLYASGKVKAIGVSNFQEHHLEELLAHADVVPAVNQVEF-HPYLTQKELRGYCKSKGIVV 178

AKR2E family of aldo-keto reductase (AKR); Bombyx mori 3-dehydroecdysone reductase is a founding member of aldo-keto reductase family 2 member E4 (AKR2E4). It is a NADP-dependent oxidoreductase with high 3-dehydroecdysone reductase activity. It may play a role in the regulation of molting and has lower activity with phenylglyoxal and isatin (in vitro). This family also includes 3-dehydroecdysone 3b-reductase from Spodoptera littoralis and Trichoplusia ni, DL-glyceraldehyde reductase from Drosophila melanogaster, aldo-keto reductase from Bombyx mori, which correspond to aldo-keto reductase family 2 member E1, E2, E3 and E5 (AKR2E1/2/3/5), respectively.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6680019  109 AVDMACSVLGVAQLDSVIMASPP--IEDGVNLSLEHLQP----Y---WEELENLVQSKKIVAIGTSDLDKTQLEQLYQWA 179
Cdd:cd19116  91 ALRESLKRLGLDYVDLYLIHWPVafKENNDSESNGDGSLsdidYletWRGMEDLVKLGLTRSIGVSNFNSEQINRLLSNC 170

                        90       100       110
                ....*....|....*....|....*....|....
gi 6680019  180 QVKPNSNQVNlasccVMP----PDLTAFAKQFDI 209
Cdd:cd19116 171 NIKPAVNQIE-----VHPtltqEKLVAYCQSNGI 199

AKR4C family of aldo-keto reductase (AKR); The AKR4C family of AKR includes aldose reductase (ALR) from Hordeum vulgare (AKR4C1), Bromus inermis (AKR4C2), Avena fatua (AKR4C3), and Xerophyta viscosa (AKR4C4), two aldose reductases, DpAR1 (AKR4C5) and DpAR2(AKR4C6), from Digitalis purpurea, aldehyde reductase from Zea mays (AKR4C7), four aldo-keto reductases from Arabidopsis thaliana (AKR4C8-11), and another three aldo-keto reductases from Aloe arborescens (AKR4C12) and Oryza sativa (AKR4C14/15). ALR (EC 1.1.1.21), also called AR, aldehyde reductase, or polyol dehydrogenase (NADP(+)), is a cytosolic NADPH-dependent oxidoreductase that catalyzes the reduction of a variety of aldehydes and carbonyls, including monosaccharides. Both DpAR1 and DpAR2 reduce the ketone group of steroid structures. They may be involved in plant steroid metabolism in general and in cardenolide biosynthesis in particular. Plant aldo-keto reductases of the AKR4C subfamily play key roles during stress and are attractive targets for developing stress-tolerant crops.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 6680019  147 WEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQV 188
Cdd:cd19125 135 WKAMEKLVDSGKVRAIGVSNFSVKKLEDLLAVARVPPAVNQV 176

Caenorhabditis elegans aldo-keto reductase (CeAKR) and similar proteins; CeAKR is a founding member of aldo-keto reductase family 1 member G1 (AKR1G1). It may catalyze the reversible reduction of ketones to the respective alcohols using NAD(P)H as a hydride donor.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 6680019  147 WEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLAscCVMP-PDLTAFAKQFDI 209
Cdd:cd19154 141 WRGMEKVYDEGLTKAIGVSNFNNDQIQRILDNARVKPHNNQVECH--LYFPqKELVEFCKKHNI 202

AKR5A family of aldo-keto reductase (AKR); Prostaglandin F2-alpha synthase (PGFS) from Leishmania major and Trypanosoma brucei are founding members of aldo-keto reductase family 5 member A1 (AKR5A1) and A2 (AKR5A2), respectively. PGFS, also called 9,11-endoperoxide prostaglandin H2 reductase, catalyzes the NADP-dependent formation of prostaglandin F2-alpha from prostaglandin H2. It has also aldo/ketoreductase activity toward the synthetic substrates 9,10-phenanthrenequinone and p-nitrobenzaldehyde.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 6680019  147 WEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPdLTAFAKQFDI 209
Cdd:cd19156 115 WKAFEKLYKEKKVRAIGVSNFHEHHLEELLKSCKVAPMVNQIELHPLLTQEP-LRKFCKEKNI 176

Giardia lamblia aldose reductase (AR) and similar proteins; Giardia lamblia AR (EC 1.1.1.21), also called aldehyde reductase, is the prototype of this family. It catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6680019  133 EDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASccVMPPD-LTAFAKQFDIQL 211
Cdd:cd19128 116 NQIQSLSKKPLEDTWRAMEQCVDEKLTKNIGVSNYSTKLLTDLLNYCKIKPFMNQIECHP--YFQNDkLIKFCIENNIHV 193

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 6680019  212 LTHN-------DPKELLSEASFQEALQE----SIPDIEAQDWVPLWLLRYSVIVKS 256
Cdd:cd19128 194 TAYRplggsygDGNLTFLNDSELKALATkyntTPPQVIIAWHLQKWPKNYSVIPKS 249

AKR5B family of aldo-keto reductase (AKR); Pseudomonas putida morphine 6-dehydrogenase (M6DH) is a founding member of the aldo-keto reductase family 5 member B1 (AKR5B1). M6DH (EC 1.1.1.218), also called naloxone reductase, oxidizes the C-6 hydroxy group of morphine and codeine.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6680019   85 EREEMKVSAKLFIVgSNSSSSTRSAVDMACSVLGVAQLDSVIMASPpiedgVNLSLEHLQPYWEELENLVQSKKIVAIGT 164
Cdd:cd19127  61 DRSDIFVTTKLWIS-DYGYDKALRGFDASLRRLGLDYVDLYLLHWP-----VPNDFDRTIQAYKALEKLLAEGRVRAIGV 134

                        90       100
                ....*....|....*....|....*.
gi 6680019  165 SDLDKTQLEQLYQWAQVKPNSNQVNL 190
Cdd:cd19127 135 SNFTPEHLERLIDATTVVPAVNQVEL 160