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Conserved domains on  [gi|53749680|ref|NP_001005435|]
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pre-mRNA-processing factor 19 [Xenopus tropicalis]

Protein Classification

pre-mRNA-processing factor 19( domain architecture ID 11616146)

pre-mRNA-processing factor 19 (PRPF19) is a ubiquitously expressed multifunctional E3 ubiquitin ligase with pleiotropic activities in DNA damage signaling, repair, and replicative senescence

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 217-431 1.97e-49

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


:

Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 169.82  E-value: 1.97e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 217 HSASVPGILALDlcptDTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDA 296
Cdd:cd00200  50 HTGPVRDVAASA----DGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSSSRDKTIKVWDVETG 125

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 297 SCAQVLRAHEGAVTGLSLHATGDYLLSCSDDQY---WAFSDIHVGRVLTKVTDEssgcaLTCAQFHPDGLIFGTGTVDSQ 373
Cdd:cd00200 126 KCLTTLRGHTDWVNSVAFSPDGTFVASSSQDGTiklWDLRTGKCVATLTGHTGE-----VNSVAFSPDGEKLLSSSSDGT 200

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 53749680 374 IKIWDLKERSNVANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:cd00200 201 IKLWDLSTGKCLGTLRGHENGVNSVAFSPDGYLLASGSEDGTIRVWDLRTGECVQTLS 258
Prp19 pfam08606
Prp19/Pso4-like; This regions is found specifically in PRP19-like protein. The region ...
 69-133 8.85e-38

Prp19/Pso4-like; This regions is found specifically in PRP19-like protein. The region represented by this family covers the sequence implicated in self-interaction and a coiled-coiled motif. PRP19-like proteins form an oligomer that is necessary for spliceosome assembly.


:

Pssm-ID: 400774  Cd Length: 65  Bit Score: 131.48  E-value: 8.85e-38
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 53749680    69 TSIPAILKSLQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALAT 133
Cdd:pfam08606   1 TSIPSLLSTLQNEWDALMLETFTLRKQLDQTRQELSHALYQHDAACRVIARLIKERDEAREALAN 65
RING-Ubox_PRP19 cd16656
U-box domain, a modified RING finger, found in pre-mRNA-processing factor 19 (Prp19) and ...
 3-56 2.17e-30

U-box domain, a modified RING finger, found in pre-mRNA-processing factor 19 (Prp19) and similar proteins; Prp19, also known as nuclear matrix protein 200 (NMP200), senescence evasion factor (SNEV), or DNA repair protein Pso4 (psoralen-sensitive mutant 4), is a ubiquitously expressed multifunctional E3 ubiquitin ligase with pleiotropic activities in DNA damage signaling, repair, and replicative senescence. It functions as a critical component of DNA repair and DNA damage checkpoint complexes. It senses DNA damage, binds double-stranded DNA in a sequence-independent manner, facilitates processing of damaged DNA, promotes DNA end joining, regulates replication protein A (RPA2) phosphorylation and ubiquitination at damaged DNA, and regulates RNA splicing and mitotic spindle formation in its integral capacity as a scaffold for a multimeric core complex. Prp19 contains an N-terminal E3 ubiquitin ligase U-box domain with E2 recruitment function that facilitates dimerization and is essential for its auto-ubiquitination activity in vitro or when overexpressed, a coiled-coil Prp19 homology region that mediates its tetramerization and interaction with CDC5L and SPF27, and a C-terminal seven-bladed WD40 beta-propeller type of leucine-rich architectural repeats that form an asymmetrical barrel-shaped structure important for substrate recognition and recruitment.


:

Pssm-ID: 438318  Cd Length: 54  Bit Score: 111.50  E-value: 2.17e-30
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 53749680   3 LTCYISNEVTDRPCISPVSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLIDIK 56
Cdd:cd16656   1 MVCAISGEVPEEPVVSPKSGHVFEKRLIEKYIAENGTDPVTGEPLTEEDLIEIK 54
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 217-431 1.97e-49

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 169.82  E-value: 1.97e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 217 HSASVPGILALDlcptDTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDA 296
Cdd:cd00200  50 HTGPVRDVAASA----DGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSSSRDKTIKVWDVETG 125

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 297 SCAQVLRAHEGAVTGLSLHATGDYLLSCSDDQY---WAFSDIHVGRVLTKVTDEssgcaLTCAQFHPDGLIFGTGTVDSQ 373
Cdd:cd00200 126 KCLTTLRGHTDWVNSVAFSPDGTFVASSSQDGTiklWDLRTGKCVATLTGHTGE-----VNSVAFSPDGEKLLSSSSDGT 200

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 53749680 374 IKIWDLKERSNVANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:cd00200 201 IKLWDLSTGKCLGTLRGHENGVNSVAFSPDGYLLASGSEDGTIRVWDLRTGECVQTLS 258
WD40 COG2319
WD40 repeat [General function prediction only];
233-431 2.31e-46

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 164.70  E-value: 2.31e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 233 DTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGL 312
Cdd:COG2319 131 DGKTLASGSADGTVRLWDLATGKLLRTLTGHSGAVTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTGAVRSV 210

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 313 SLHATGDYLLSCSDDQYWAFSDIHVGRVLTKVTDESSGcaLTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHS 392
Cdd:COG2319 211 AFSPDGKLLASGSADGTVRLWDLATGKLLRTLTGHSGS--VRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGHS 288

                       170       180       190
                ....*....|....*....|....*....|....*....
gi 53749680 393 GPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:COG2319 289 GGVNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLT 327
Prp19 pfam08606
Prp19/Pso4-like; This regions is found specifically in PRP19-like protein. The region ...
 69-133 8.85e-38

Prp19/Pso4-like; This regions is found specifically in PRP19-like protein. The region represented by this family covers the sequence implicated in self-interaction and a coiled-coiled motif. PRP19-like proteins form an oligomer that is necessary for spliceosome assembly.


Pssm-ID: 400774  Cd Length: 65  Bit Score: 131.48  E-value: 8.85e-38
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 53749680    69 TSIPAILKSLQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALAT 133
Cdd:pfam08606   1 TSIPSLLSTLQNEWDALMLETFTLRKQLDQTRQELSHALYQHDAACRVIARLIKERDEAREALAN 65
RING-Ubox_PRP19 cd16656
U-box domain, a modified RING finger, found in pre-mRNA-processing factor 19 (Prp19) and ...
 3-56 2.17e-30

U-box domain, a modified RING finger, found in pre-mRNA-processing factor 19 (Prp19) and similar proteins; Prp19, also known as nuclear matrix protein 200 (NMP200), senescence evasion factor (SNEV), or DNA repair protein Pso4 (psoralen-sensitive mutant 4), is a ubiquitously expressed multifunctional E3 ubiquitin ligase with pleiotropic activities in DNA damage signaling, repair, and replicative senescence. It functions as a critical component of DNA repair and DNA damage checkpoint complexes. It senses DNA damage, binds double-stranded DNA in a sequence-independent manner, facilitates processing of damaged DNA, promotes DNA end joining, regulates replication protein A (RPA2) phosphorylation and ubiquitination at damaged DNA, and regulates RNA splicing and mitotic spindle formation in its integral capacity as a scaffold for a multimeric core complex. Prp19 contains an N-terminal E3 ubiquitin ligase U-box domain with E2 recruitment function that facilitates dimerization and is essential for its auto-ubiquitination activity in vitro or when overexpressed, a coiled-coil Prp19 homology region that mediates its tetramerization and interaction with CDC5L and SPF27, and a C-terminal seven-bladed WD40 beta-propeller type of leucine-rich architectural repeats that form an asymmetrical barrel-shaped structure important for substrate recognition and recruitment.


Pssm-ID: 438318  Cd Length: 54  Bit Score: 111.50  E-value: 2.17e-30
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 53749680   3 LTCYISNEVTDRPCISPVSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLIDIK 56
Cdd:cd16656   1 MVCAISGEVPEEPVVSPKSGHVFEKRLIEKYIAENGTDPVTGEPLTEEDLIEIK 54
Ubox smart00504
Modified RING finger domain; Modified RING finger domain, without the full complement of Zn2 ...
 5-68 5.71e-19

Modified RING finger domain; Modified RING finger domain, without the full complement of Zn2+-binding ligands. Probable involvement in E2-dependent ubiquitination.


Pssm-ID: 128780 [Multi-domain]  Cd Length: 63  Bit Score: 80.36  E-value: 5.71e-19
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 53749680      5 CYISNEVTDRPCISPvSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLIDIkvaHPIRPKPPSA 68
Cdd:smart00504   4 CPISLEVMKDPVILP-SGQTYERSAIEKWLLSHGTDPVTGQPLTHEDLIPN---LALKSAIQEW 63
PTZ00421 PTZ00421
coronin; Provisional
 231-381 4.34e-11

coronin; Provisional


Pssm-ID: 173611 [Multi-domain]  Cd Length: 493  Bit Score: 64.53  E-value: 4.34e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680  231 PTDTNKTLTGGADKSVVVF----DALSQQI---LATLKGHTKKVTSVVFHPSQELVF-SASPDSTIRVWSVPDASCAQVL 302
Cdd:PTZ00421  85 PFDPQKLFTASEDGTIMGWgipeEGLTQNIsdpIVHLQGHTKKVGIVSFHPSAMNVLaSAGADMVVNVWDVERGKAVEVI 164

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680  303 RAHEGAVTGLSLHATGDYLLSCSDDQYWAFSDIHVGRVLTKVTDESSGCALTCAQFHPDGLIFGTGTVDS---QIKIWDL 379
Cdd:PTZ00421 165 KCHSDQITSLEWNLDGSLLCTTSKDKKLNIIDPRDGTIVSSVEAHASAKSQRCLWAKRKDLIITLGCSKSqqrQIMLWDT 244


                 ..
gi 53749680  380 KE 381
Cdd:PTZ00421 245 RK 246
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 385-420 8.39e-10

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 53.86  E-value: 8.39e-10
                           10        20        30
                   ....*....|....*....|....*....|....*.
gi 53749680    385 VANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWD 420
Cdd:smart00320   5 LKTLKGHTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
385-420 3.66e-09

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 51.96  E-value: 3.66e-09
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 53749680   385 VANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWD 420
Cdd:pfam00400   4 LKTLEGHTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
U-box pfam04564
U-box domain; The U-box is a domain of ~70 amino acids that is present in proteins from yeast ...
 5-53 1.42e-07

U-box domain; The U-box is a domain of ~70 amino acids that is present in proteins from yeast to human. It consists of the beta-beta-alpha-beta-alpha- fold typical of U-box and RING domains. The central alpha helix is flanked by two prominent surface-exposed loop regions. This domain is one class of E3 ligases, involved in the ubiquitination process. This domain is related to the Ring finger pfam00097 but lacks the zinc binding residues.


Pssm-ID: 398320 [Multi-domain]  Cd Length: 73  Bit Score: 48.46  E-value: 1.42e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 53749680     5 CYISNEVTDRPCISPvSGHIFDRRLIEKYLAENG-TDPVTNQPLSEDQLI 53
Cdd:pfam04564   7 DPITFELMTDPVILP-SGITYDRSTIERHLLSVDpTDPFTREPLTHDQLI 55
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 217-431 1.97e-49

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 169.82  E-value: 1.97e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 217 HSASVPGILALDlcptDTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDA 296
Cdd:cd00200  50 HTGPVRDVAASA----DGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSSSRDKTIKVWDVETG 125

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 297 SCAQVLRAHEGAVTGLSLHATGDYLLSCSDDQY---WAFSDIHVGRVLTKVTDEssgcaLTCAQFHPDGLIFGTGTVDSQ 373
Cdd:cd00200 126 KCLTTLRGHTDWVNSVAFSPDGTFVASSSQDGTiklWDLRTGKCVATLTGHTGE-----VNSVAFSPDGEKLLSSSSDGT 200

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 53749680 374 IKIWDLKERSNVANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:cd00200 201 IKLWDLSTGKCLGTLRGHENGVNSVAFSPDGYLLASGSEDGTIRVWDLRTGECVQTLS 258
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 223-431 1.09e-48

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 167.90  E-value: 1.09e-48
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 223 GILALDLCPtDTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVL 302
Cdd:cd00200  11 GVTCVAFSP-DGKLLATGSGDGTIKVWDLETGELLRTLKGHTGPVRDVAASADGTYLASGSSDKTIRLWDLETGECVRTL 89

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 303 RAHEGAVTGLSLHATGDYLLSCSDDQYWAFSDIHVGRVLTKVTDESsgCALTCAQFHPDGLIFGTGTVDSQIKIWDLKER 382
Cdd:cd00200  90 TGHTSYVSSVAFSPDGRILSSSSRDKTIKVWDVETGKCLTTLRGHT--DWVNSVAFSPDGTFVASSSQDGTIKLWDLRTG 167

                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*....
gi 53749680 383 SNVANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:cd00200 168 KCVATLTGHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLR 216
WD40 COG2319
WD40 repeat [General function prediction only];
233-431 2.31e-46

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 164.70  E-value: 2.31e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 233 DTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGL 312
Cdd:COG2319 131 DGKTLASGSADGTVRLWDLATGKLLRTLTGHSGAVTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTGAVRSV 210

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 313 SLHATGDYLLSCSDDQYWAFSDIHVGRVLTKVTDESSGcaLTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHS 392
Cdd:COG2319 211 AFSPDGKLLASGSADGTVRLWDLATGKLLRTLTGHSGS--VRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGHS 288

                       170       180       190
                ....*....|....*....|....*....|....*....
gi 53749680 393 GPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:COG2319 289 GGVNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLT 327
WD40 COG2319
WD40 repeat [General function prediction only];
236-431 2.04e-44

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 159.69  E-value: 2.04e-44
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 236 KTL-TGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGLSL 314
Cdd:COG2319 175 KLLaSGSDDGTVRLWDLATGKLLRTLTGHTGAVRSVAFSPDGKLLASGSADGTVRLWDLATGKLLRTLTGHSGSVRSVAF 254

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 315 HATGDYLLSCSDDQYWAFSDIHVGRVLTKVTDESSGcaLTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHSGP 394
Cdd:COG2319 255 SPDGRLLASGSADGTVRLWDLATGELLRTLTGHSGG--VNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTGA 332

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 53749680 395 VSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:COG2319 333 VRSVAFSPDGKTLASGSDDGTVRLWDLATGELLRTLT 369
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 238-420 1.42e-43

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 154.42  E-value: 1.42e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 238 LTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGLSLHAT 317
Cdd:cd00200 109 SSSSRDKTIKVWDVETGKCLTTLRGHTDWVNSVAFSPDGTFVASSSQDGTIKLWDLRTGKCVATLTGHTGEVNSVAFSPD 188

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 318 GDYLLSCSDDQ---YWAFSDIHVGRVLTKVTDessgcALTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHSGP 394
Cdd:cd00200 189 GEKLLSSSSDGtikLWDLSTGKCLGTLRGHEN-----GVNSVAFSPDGYLLASGSEDGTIRVWDLRTGECVQTLSGHTNS 263

                       170       180
                ....*....|....*....|....*.
gi 53749680 395 VSCIAFSENGYYLATAADDSSVKLWD 420
Cdd:cd00200 264 VTSLAWSPDGKRLASGSADGTIRIWD 289
WD40 COG2319
WD40 repeat [General function prediction only];
239-422 2.19e-43

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 157.00  E-value: 2.19e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 239 TGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGLSLHATG 318
Cdd:COG2319 221 SGSADGTVRLWDLATGKLLRTLTGHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGHSGGVNSVAFSPDG 300

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 319 DYLLSCSDDQYWAFSDIHVGRVLTKVTDESSgcALTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHSGPVSCI 398
Cdd:COG2319 301 KLLASGSDDGTVRLWDLATGKLLRTLTGHTG--AVRSVAFSPDGKTLASGSDDGTVRLWDLATGELLRTLTGHTGAVTSV 378

                       170       180
                ....*....|....*....|....
gi 53749680 399 AFSENGYYLATAADDSSVKLWDLR 422
Cdd:COG2319 379 AFSPDGRTLASGSADGTVRLWDLA 402
WD40 COG2319
WD40 repeat [General function prediction only];
233-431 3.08e-43

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 156.61  E-value: 3.08e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 233 DTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGL 312
Cdd:COG2319  89 DGRLLASASADGTVRLWDLATGLLLRTLTGHTGAVRSVAFSPDGKTLASGSADGTVRLWDLATGKLLRTLTGHSGAVTSV 168

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 313 SLHATGDYLLSCSDD---QYWafsDIHVGRVLTKVTDESSgcALTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFP 389
Cdd:COG2319 169 AFSPDGKLLASGSDDgtvRLW---DLATGKLLRTLTGHTG--AVRSVAFSPDGKLLASGSADGTVRLWDLATGKLLRTLT 243

                       170       180       190       200
                ....*....|....*....|....*....|....*....|..
gi 53749680 390 GHSGPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:COG2319 244 GHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLT 285
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 257-436 1.93e-41

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 148.64  E-value: 1.93e-41
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 257 LATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGLSLHATGDYLLSCSDDQY---WAFS 333
Cdd:cd00200   2 RRTLKGHTGGVTCVAFSPDGKLLATGSGDGTIKVWDLETGELLRTLKGHTGPVRDVAASADGTYLASGSSDKTirlWDLE 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 334 DIHVGRVLTKVTDEssgcaLTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHSGPVSCIAFSENGYYLATAADD 413
Cdd:cd00200  82 TGECVRTLTGHTSY-----VSSVAFSPDGRILSSSSRDKTIKVWDVETGKCLTTLRGHTDWVNSVAFSPDGTFVASSSQD 156

                       170       180
                ....*....|....*....|...
gi 53749680 414 SSVKLWDLRKLKNFKTLQLEEGY 436
Cdd:cd00200 157 GTIKLWDLRTGKCVATLTGHTGE 179
Prp19 pfam08606
Prp19/Pso4-like; This regions is found specifically in PRP19-like protein. The region ...
 69-133 8.85e-38

Prp19/Pso4-like; This regions is found specifically in PRP19-like protein. The region represented by this family covers the sequence implicated in self-interaction and a coiled-coiled motif. PRP19-like proteins form an oligomer that is necessary for spliceosome assembly.


Pssm-ID: 400774  Cd Length: 65  Bit Score: 131.48  E-value: 8.85e-38
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 53749680    69 TSIPAILKSLQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALAT 133
Cdd:pfam08606   1 TSIPSLLSTLQNEWDALMLETFTLRKQLDQTRQELSHALYQHDAACRVIARLIKERDEAREALAN 65
WD40 COG2319
WD40 repeat [General function prediction only];
214-436 4.08e-35

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 134.27  E-value: 4.08e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 214 VGLHSASVPGILALDLCPTDTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSV 293
Cdd:COG2319  28 LLLLLLGLAAAVASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPDGRLLASASADGTVRLWDL 107

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 294 PDASCAQVLRAHEGAVTGLSLHATGDYLLSCSDDQY---WafsDIHVGRVLTKVTDESSgcALTCAQFHPDGLIFGTGTV 370
Cdd:COG2319 108 ATGLLLRTLTGHTGAVRSVAFSPDGKTLASGSADGTvrlW---DLATGKLLRTLTGHSG--AVTSVAFSPDGKLLASGSD 182

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 53749680 371 DSQIKIWDLKERSNVANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLEEGY 436
Cdd:COG2319 183 DGTVRLWDLATGKLLRTLTGHTGAVRSVAFSPDGKLLASGSADGTVRLWDLATGKLLRTLTGHSGS 248
RING-Ubox_PRP19 cd16656
U-box domain, a modified RING finger, found in pre-mRNA-processing factor 19 (Prp19) and ...
 3-56 2.17e-30

U-box domain, a modified RING finger, found in pre-mRNA-processing factor 19 (Prp19) and similar proteins; Prp19, also known as nuclear matrix protein 200 (NMP200), senescence evasion factor (SNEV), or DNA repair protein Pso4 (psoralen-sensitive mutant 4), is a ubiquitously expressed multifunctional E3 ubiquitin ligase with pleiotropic activities in DNA damage signaling, repair, and replicative senescence. It functions as a critical component of DNA repair and DNA damage checkpoint complexes. It senses DNA damage, binds double-stranded DNA in a sequence-independent manner, facilitates processing of damaged DNA, promotes DNA end joining, regulates replication protein A (RPA2) phosphorylation and ubiquitination at damaged DNA, and regulates RNA splicing and mitotic spindle formation in its integral capacity as a scaffold for a multimeric core complex. Prp19 contains an N-terminal E3 ubiquitin ligase U-box domain with E2 recruitment function that facilitates dimerization and is essential for its auto-ubiquitination activity in vitro or when overexpressed, a coiled-coil Prp19 homology region that mediates its tetramerization and interaction with CDC5L and SPF27, and a C-terminal seven-bladed WD40 beta-propeller type of leucine-rich architectural repeats that form an asymmetrical barrel-shaped structure important for substrate recognition and recruitment.


Pssm-ID: 438318  Cd Length: 54  Bit Score: 111.50  E-value: 2.17e-30
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 53749680   3 LTCYISNEVTDRPCISPVSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLIDIK 56
Cdd:cd16656   1 MVCAISGEVPEEPVVSPKSGHVFEKRLIEKYIAENGTDPVTGEPLTEEDLIEIK 54
WD40 COG2319
WD40 repeat [General function prediction only];
238-431 2.45e-27

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 112.70  E-value: 2.45e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 238 LTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGLSLHAT 317
Cdd:COG2319  10 AAASADLALALLAAALGALLLLLLGLAAAVASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPD 89

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 318 GDYLLSCSDD---QYWAFSDIHVGRVLTKVTDESSGCAltcaqFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHSGP 394
Cdd:COG2319  90 GRLLASASADgtvRLWDLATGLLLRTLTGHTGAVRSVA-----FSPDGKTLASGSADGTVRLWDLATGKLLRTLTGHSGA 164

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 53749680 395 VSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQ 431
Cdd:COG2319 165 VTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLT 201
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 224-328 7.75e-23

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 97.79  E-value: 7.75e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 224 ILALDLCPtDTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLR 303
Cdd:cd00200 180 VNSVAFSP-DGEKLLSSSSDGTIKLWDLSTGKCLGTLRGHENGVNSVAFSPDGYLLASGSEDGTIRVWDLRTGECVQTLS 258

                        90       100
                ....*....|....*....|....*
gi 53749680 304 AHEGAVTGLSLHATGDYLLSCSDDQ 328
Cdd:cd00200 259 GHTNSVTSLAWSPDGKRLASGSADG 283
WD40 COG2319
WD40 repeat [General function prediction only];
238-328 1.07e-20

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 93.44  E-value: 1.07e-20
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 238 LTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGLSLHAT 317
Cdd:COG2319 304 ASGSDDGTVRLWDLATGKLLRTLTGHTGAVRSVAFSPDGKTLASGSDDGTVRLWDLATGELLRTLTGHTGAVTSVAFSPD 383

                        90
                ....*....|.
gi 53749680 318 GDYLLSCSDDQ 328
Cdd:COG2319 384 GRTLASGSADG 394
Ubox smart00504
Modified RING finger domain; Modified RING finger domain, without the full complement of Zn2 ...
 5-68 5.71e-19

Modified RING finger domain; Modified RING finger domain, without the full complement of Zn2+-binding ligands. Probable involvement in E2-dependent ubiquitination.


Pssm-ID: 128780 [Multi-domain]  Cd Length: 63  Bit Score: 80.36  E-value: 5.71e-19
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 53749680      5 CYISNEVTDRPCISPvSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLIDIkvaHPIRPKPPSA 68
Cdd:smart00504   4 CPISLEVMKDPVILP-SGQTYERSAIEKWLLSHGTDPVTGQPLTHEDLIPN---LALKSAIQEW 63
WD40 COG2319
WD40 repeat [General function prediction only];
271-431 1.74e-18

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 86.89  E-value: 1.74e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 271 VFHPSQELVFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGLSLHATGDYLLSCSDDQYWAFSDIHVGRVLTKVTDESSg 350
Cdd:COG2319   1 ALSADGAALAAASADLALALLAAALGALLLLLLGLAAAVASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTA- 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 351 cALTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWDLRKLKNFKTL 430
Cdd:COG2319  80 -AVLSVAFSPDGRLLASASADGTVRLWDLATGLLLRTLTGHTGAVRSVAFSPDGKTLASGSADGTVRLWDLATGKLLRTL 158


                .
gi 53749680 431 Q 431
Cdd:COG2319 159 T 159
WD40 COG2319
WD40 repeat [General function prediction only];
217-295 2.31e-12

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 68.40  E-value: 2.31e-12
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 53749680 217 HSASVPGILALDLCPtDTNKTLTGGADKSVVVFDALSQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWSVPD 295
Cdd:COG2319 326 LTGHTGAVRSVAFSP-DGKTLASGSDDGTVRLWDLATGELLRTLTGHTGAVTSVAFSPDGRTLASGSADGTVRLWDLAT 403
RING-Ubox_CHIP cd16654
U-box domain, a modified RING finger, found in carboxyl terminus of HSP70-interacting protein ...
 3-53 2.38e-11

U-box domain, a modified RING finger, found in carboxyl terminus of HSP70-interacting protein (CHIP) and similar proteins; CHIP, also known as STIP1 homology and U box-containing protein 1 (STUB1), CLL-associated antigen KW-8, or Antigen NY-CO-7, is a multifunctional protein that functions both as a co-chaperone and an E3 ubiquitin-protein ligase. It couples protein folding and proteasome mediated degradation by interacting with heat shock proteins (e.g. HSC70) and ubiquitinating their misfolded client proteins, thereby targeting them for proteasomal degradation. It is also important for cellular differentiation and survival (or apoptosis), as well as susceptibility to stress. It targets a wide range of proteins, such as expanded ataxin-1, ataxin-3, huntingtin, and androgen receptor, which play roles in glucocorticoid response, tau degradation, and both p53 and cAMP signaling. CHIP contains an N-terminal tetratricopeptide repeat (TPR) domain responsible for protein-protein interaction, a highly charged middle coiled-coil (CC), and a C-terminal RING-like U-box domain acting as an ubiquitin ligase.


Pssm-ID: 438316 [Multi-domain]  Cd Length: 71  Bit Score: 59.13  E-value: 2.38e-11
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|..
gi 53749680   3 LTCYISNEVTDRPCISPvSGHIFDRRLIEKYLAENG-TDPVTNQPLSEDQLI 53
Cdd:cd16654   5 LCCKISFELMRDPVITP-SGITYERKDIEEHLQRVGhFDPITREPLTQDQLI 55
PTZ00421 PTZ00421
coronin; Provisional
 231-381 4.34e-11

coronin; Provisional


Pssm-ID: 173611 [Multi-domain]  Cd Length: 493  Bit Score: 64.53  E-value: 4.34e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680  231 PTDTNKTLTGGADKSVVVF----DALSQQI---LATLKGHTKKVTSVVFHPSQELVF-SASPDSTIRVWSVPDASCAQVL 302
Cdd:PTZ00421  85 PFDPQKLFTASEDGTIMGWgipeEGLTQNIsdpIVHLQGHTKKVGIVSFHPSAMNVLaSAGADMVVNVWDVERGKAVEVI 164

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680  303 RAHEGAVTGLSLHATGDYLLSCSDDQYWAFSDIHVGRVLTKVTDESSGCALTCAQFHPDGLIFGTGTVDS---QIKIWDL 379
Cdd:PTZ00421 165 KCHSDQITSLEWNLDGSLLCTTSKDKKLNIIDPRDGTIVSSVEAHASAKSQRCLWAKRKDLIITLGCSKSqqrQIMLWDT 244


                 ..
gi 53749680  380 KE 381
Cdd:PTZ00421 245 RK 246
RING-Ubox cd16453
U-box domain, a modified RING finger; The U-box protein family is a family of E3 enzymes that ...
 3-47 4.54e-11

U-box domain, a modified RING finger; The U-box protein family is a family of E3 enzymes that also includes the HECT family and the RING finger family. The E3 enzyme is ubiquitin-protein ligase that cooperates with a ubiquitin-activating enzyme (E1) and a ubiquitin-conjugating enzyme (E2), and plays a central role in determining the specificity of the ubiquitination system. It removes the ubiquitin molecule from the E2 enzyme and attaches it to the target substrate, forming a covalent bond between ubiquitin and the target. U-box proteins are characterized by the presence of a U-box domain of approximately 70 amino acids. The U-box is a modified form of the RING finger domain that lacks metal chelating cysteines and histidines. It resembles the cross-brace RING structure consisting of three beta-sheets and a single alpha-helix, which would be stabilized by salt bridges instead of chelated metal ions. U-box proteins are widely distributed among eukaryotic organisms and show a higher prevalence in plants than in other organisms.


Pssm-ID: 438117 [Multi-domain]  Cd Length: 44  Bit Score: 57.56  E-value: 4.54e-11
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*
gi 53749680   3 LTCYISNEVTDRPCISPvSGHIFDRRLIEKYLAENGTDPVTNQPL 47
Cdd:cd16453   1 FLCPISGELMKDPVITP-SGITYDRSAIERWLLSDNTDPFTREPL 44
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 385-420 8.39e-10

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 53.86  E-value: 8.39e-10
                           10        20        30
                   ....*....|....*....|....*....|....*.
gi 53749680    385 VANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWD 420
Cdd:smart00320   5 LKTLKGHTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
RING-Ubox_WDSUB1-like cd16655
U-box domain, a modified RING finger, found in WD repeat, SAM and U-box domain-containing ...
 2-53 2.85e-09

U-box domain, a modified RING finger, found in WD repeat, SAM and U-box domain-containing protein 1 (WDSUB1) and similar proteins; WDSUB1 is an uncharacterized protein containing seven WD40 repeats and a SAM domain in addition to the U-box. Its biological role remains unclear. This subfamily also includes many uncharacterized kinase domain-containing U-box (AtPUB) proteins and several MIF4G motif-containing AtPUB proteins from Arabidopsis.


Pssm-ID: 438317 [Multi-domain]  Cd Length: 55  Bit Score: 52.89  E-value: 2.85e-09
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|..
gi 53749680   2 SLTCYISNEVTDRPCISPvSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLI 53
Cdd:cd16655   3 EFLCPITQELMRDPVVAA-DGHTYERSAIEEWLETHNTSPMTRLPLSSTDLV 53
WD40 pfam00400
WD domain, G-beta repeat;
385-420 3.66e-09

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 51.96  E-value: 3.66e-09
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 53749680   385 VANFPGHSGPVSCIAFSENGYYLATAADDSSVKLWD 420
Cdd:pfam00400   4 LKTLEGHTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 253-292 6.55e-09

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 51.16  E-value: 6.55e-09
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|
gi 53749680    253 SQQILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWS 292
Cdd:smart00320   1 SGELLKTLKGHTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
255-292 9.19e-09

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 50.81  E-value: 9.19e-09
                          10        20        30
                  ....*....|....*....|....*....|....*...
gi 53749680   255 QILATLKGHTKKVTSVVFHPSQELVFSASPDSTIRVWS 292
Cdd:pfam00400   2 KLLKTLEGHTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
PLN00181 PLN00181
protein SPA1-RELATED; Provisional
 279-436 1.78e-08

protein SPA1-RELATED; Provisional


Pssm-ID: 177776 [Multi-domain]  Cd Length: 793  Bit Score: 56.63  E-value: 1.78e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680  279 VFSASPDSTIRVWSVPDASCAQVLRAHEGAVTGLSLHATGDYLL-SCSDDQYWAFSDIHVGRVLTKVTDESSGCaltCAQ 357
Cdd:PLN00181 548 VASSNFEGVVQVWDVARSQLVTEMKEHEKRVWSIDYSSADPTLLaSGSDDGSVKLWSINQGVSIGTIKTKANIC---CVQ 624

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680  358 FHPD-GLIFGTGTVDSQIKIWDLKE-RSNVANFPGHSGPVSCIAFSENGYyLATAADDSSVKLWDLR------------- 422
Cdd:PLN00181 625 FPSEsGRSLAFGSADHKVYYYDLRNpKLPLCTMIGHSKTVSYVRFVDSST-LVSSSTDNTLKLWDLSmsisginetplhs 703

                        170
                 ....*....|....*....
gi 53749680  423 -----KLKNFKTLQLEEGY 436
Cdd:PLN00181 704 fmghtNVKNFVGLSVSDGY 722
U-box pfam04564
U-box domain; The U-box is a domain of ~70 amino acids that is present in proteins from yeast ...
 5-53 1.42e-07

U-box domain; The U-box is a domain of ~70 amino acids that is present in proteins from yeast to human. It consists of the beta-beta-alpha-beta-alpha- fold typical of U-box and RING domains. The central alpha helix is flanked by two prominent surface-exposed loop regions. This domain is one class of E3 ligases, involved in the ubiquitination process. This domain is related to the Ring finger pfam00097 but lacks the zinc binding residues.


Pssm-ID: 398320 [Multi-domain]  Cd Length: 73  Bit Score: 48.46  E-value: 1.42e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 53749680     5 CYISNEVTDRPCISPvSGHIFDRRLIEKYLAENG-TDPVTNQPLSEDQLI 53
Cdd:pfam04564   7 DPITFELMTDPVILP-SGITYDRSTIERHLLSVDpTDPFTREPLTHDQLI 55
RING-Ubox_PUB cd16664
U-box domain, a modified RING finger, found in Arabidopsis plant U-box proteins (AtPUB) and ...
 5-52 1.59e-07

U-box domain, a modified RING finger, found in Arabidopsis plant U-box proteins (AtPUB) and similar proteins; The plant PUB proteins, also known as U-box domain-containing proteins, are much more numerous in Arabidopsis which has 62 in comparison with the typical 6 in most animals. The majority of AtPUBs in this subfamily are known as ARM domain-containing PUB proteins, containing a C-terminally-located, tandem ARM (armadillo) repeat protein-interaction region in addition to the U-box domain. They have been implicated in the regulation of cell death and defense. They also play important roles in other plant-specific pathways, such as controlling both self-incompatibility and pseudo-self-incompatibility, as well as acting in abiotic stress. A subgroup of ARM domain-containing PUB proteins harbors a plant-specific U-box N-terminal domain.


Pssm-ID: 438326 [Multi-domain]  Cd Length: 53  Bit Score: 47.56  E-value: 1.59e-07
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*....
gi 53749680   5 CYISNEVTDRPCISPvSGHIFDRRLIEKYLAENG-TDPVTNQPLSEDQL 52
Cdd:cd16664   6 CPISLELMKDPVILA-TGQTYERAAIEKWLDSGNnTCPITGQPLTHTDL 53
RING-Ubox_LubX-like_rpt1 cd23149
first U-box domain, a modified RING finger, found in Legionella pneumophila U-box protein LubX ...
 3-53 1.83e-07

first U-box domain, a modified RING finger, found in Legionella pneumophila U-box protein LubX and similar proteins; LubX, also called RING-type E3 ubiquitin transferase LubX, is part of the large arsenal of effectors in Legionella pneumophila that are translocated into the host cytosol during infection. LubX acts as an E3 ubiquitin-protein ligase (EC 2.3.2.27) that interferes with the host's ubiquitination pathway. LubX contains two RING-like U-box domains. U-box 1 is critical to the ubiquitin ligase activity, and U-box 2 mediates interaction with host target. This model corresponds to the first one.


Pssm-ID: 438511 [Multi-domain]  Cd Length: 55  Bit Score: 47.48  E-value: 1.83e-07
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 53749680   3 LTCYISNEVTDRPCISPvSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLI 53
Cdd:cd23149   1 FTCPITSGFMEDPVITP-SGFSYERSAIERWLETKPEDPQTREPLTAKDLQ 50
PTZ00420 PTZ00420
coronin; Provisional
 260-422 4.14e-06

coronin; Provisional


Pssm-ID: 240412 [Multi-domain]  Cd Length: 568  Bit Score: 49.18  E-value: 4.14e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680  260 LKGHTKKVTSVVFHPS-QELVFSASPDSTIRVWSVP----------DASCaqVLRAHEGAVTGLSLHATGDYLLSCSD-D 327
Cdd:PTZ00420  70 LKGHTSSILDLQFNPCfSEILASGSEDLTIRVWEIPhndesvkeikDPQC--ILKGHKKKISIIDWNPMNYYIMCSSGfD 147

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680  328 QYWAFSDIHVGRVLTKVTDESSgcaLTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHSGPVS--CIAFS---- 401
Cdd:PTZ00420 148 SFVNIWDIENEKRAFQINMPKK---LSSLKWNIKGNLLSGTCVGKHMHIIDPRKQEIASSFHIHDGGKNtkNIWIDglgg 224

                        170       180
                 ....*....|....*....|....
gi 53749680  402 ENGYYLATAADDSS---VKLWDLR 422
Cdd:PTZ00420 225 DDNYILSTGFSKNNmreMKLWDLK 248
RING-Ubox_UBE4B cd16658
U-box domain, a modified RING finger, found in ubiquitin conjugation factor E4 B (UBE4B) and ...
 15-53 1.04e-05

U-box domain, a modified RING finger, found in ubiquitin conjugation factor E4 B (UBE4B) and similar proteins; UBE4B, also known as UFD2a, is a U-box-type ubiquitin-protein ligase that functions as an E3 ubiquitin ligase and an E4 polyubiquitin chain elongation factor, which catalyzes formation of Lys27- and Lys33-linked polyubiquitin chains rather than the Lys48-linked chain. It is a mammalian homolog of yeast UFD2 ubiquitination factor and it participates in the proteasomal degradation of misfolded or damaged proteins through association with chaperones. It is located in common neuroblastoma deletion regions and may be subject to mutations in tumors. UBE4B has contradictory functions upon tumorigenesis as an oncogene or tumor suppressor in different types of cancers. It is essential for Hdm2 (also known as Mdm2)-mediated p53 degradation. It mediates p53 polyubiquitination and degradation, as well as inhibits p53-dependent transactivation and apoptosis, and thus plays an important role in regulating phosphorylated p53 following DNA damage. UBE4B is also associated with other pathways independent of the p53 family, such as polyglutamine aggregation and Wallerian degeneration, both of which are critical in neurodegenerative diseases. Moreover, UBE4B acts as a regulator of epidermal growth factor receptor (EGFR) degradation. It is recruited to endosomes in response to EGFR activation by binding to Hrs, a key component of endosomal sorting complex required for transport (ESCRT) 0, and then regulates endosomal sorting, affecting cellular levels of the EGFR and its downstream signaling. UBE4B contains a ubiquitin elongating factor core and a RING-like U-box domain at the C-terminus.


Pssm-ID: 438320  Cd Length: 74  Bit Score: 43.42  E-value: 1.04e-05
                        10        20        30
                ....*....|....*....|....*....|....*....
gi 53749680  15 PCISPvSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLI 53
Cdd:cd16658  20 PVILP-SGTIMDRSIILRHLLNSQTDPFNRQPLTEDMLE 57
RING-Ubox_LubX-like_rpt2 cd23150
second U-box domain, a modified RING finger, found in Legionella pneumophila U-box protein ...
 2-82 5.38e-05

second U-box domain, a modified RING finger, found in Legionella pneumophila U-box protein LubX and similar proteins; LubX, also called RING-type E3 ubiquitin transferase LubX, is part of the large arsenal of effectors in Legionella pneumophila that are translocated into the host cytosol during infection. LubX acts as an E3 ubiquitin-protein ligase (EC 2.3.2.27) that interferes with the host's ubiquitination pathway. LubX contains two RING-like U-box domains. U-box 1 is critical to the ubiquitin ligase activity, and U-box 2 mediates interaction with host target. This model corresponds to the second one.


Pssm-ID: 438512 [Multi-domain]  Cd Length: 69  Bit Score: 41.30  E-value: 5.38e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680   2 SLTCYISNEVTDRPCISPvSGHIFDRRLIEKYLAENGTDPVTNQPLsedQLidikvaHPIRPKppsatsipAILKSLQDE 81
Cdd:cd23150   3 IFLCPISKTLIKTPVITA-QGKVYDQEALSNFLIATGNKDETGKKL---SI------DDVVVF--------DELYQQIKV 64


                .
gi 53749680  82 W 82
Cdd:cd23150  65 Y 65
RING-Ubox_PPIL2 cd16663
U-box domain, a modified RING finger, found in peptidyl-prolyl cis-trans isomerase-like 2 ...
 5-56 6.84e-05

U-box domain, a modified RING finger, found in peptidyl-prolyl cis-trans isomerase-like 2 (PPIL2) and similar proteins; PPIL2 (EC 5.2.1.8), also known as PPIase, CYC4, cyclophilin-60 (Cyp60), cyclophilin-like protein Cyp-60, or Rotamase PPIL2, is a nuclear-specific cyclophilin which interacts with the proteinase inhibitor eglin c and regulates gene expression. PPIL2 belongs to the cyclophilin family of peptidylprolyl isomerases and catalyzes cis-trans isomerization of proline-peptide bonds, which is often a rate-limiting step in protein folding. It positively regulates beta-site amyloid precursor protein cleaving enzyme (BACE1) expression and beta-secretase activity. Moreover, PPIL2 plays an important role in the translocation of CD147 to the cell surface, and thus may present a novel target for therapeutic interventions in diseases where CD147 functions as a pathogenic factor in cancer, human immunodeficiency virus infection, or rheumatoid arthritis. PPIL2 contains an N-terminal RING-like U-box domain and a C-terminal cyclophilin (Cyp)-like chaperone domain.


Pssm-ID: 438325  Cd Length: 73  Bit Score: 41.01  E-value: 6.84e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|..
gi 53749680   5 CYISNEVTDRPCISPvSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLIDIK 56
Cdd:cd16663   5 CALSLQPFENPVCTP-DGIVFDLLNIVPYLKKYGKNPVTGEPLEAKDLIKLN 55
CDC55 COG5170
Serine/threonine protein phosphatase 2A, regulatory subunit [Signal transduction mechanisms];
298-434 1.14e-04

Serine/threonine protein phosphatase 2A, regulatory subunit [Signal transduction mechanisms];


Pssm-ID: 227498 [Multi-domain]  Cd Length: 460  Bit Score: 44.25  E-value: 1.14e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 298 CAQVLRAHEGAVTGLSLHATGDYLLSCSD-----------DQYWAFSDI--HVGRVLTKVtdessgcaLTCAQFHP-DGL 363
Cdd:COG5170 164 CRVYANAHPYHINSISFNSDKETLLSADDlrinlwnleiiDGSFNIVDIkpHNMEELTEV--------ITSAEFHPeMCN 235

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 53749680 364 IFGTGTVDSQIKIWDLKERS----------------NVANFPGHSGPVSCIAFSENGYYLAtAADDSSVKLWDLRKLKN- 426
Cdd:COG5170 236 VFMYSSSKGEIKLNDLRQSAlcdnskklfeltidgvDVDFFEEIVSSISDFKFSDNGRYIL-SRDYLTVKIWDVNMAKNp 314


                ....*...
gi 53749680 427 FKTLQLEE 434
Cdd:COG5170 315 IKTIPMHC 322
RING-Ubox_UBE4A cd16657
U-box domain, a modified RING finger, found in ubiquitin conjugation factor E4 A (UBE4A) and ...
 15-54 1.28e-04

U-box domain, a modified RING finger, found in ubiquitin conjugation factor E4 A (UBE4A) and similar proteins; This subfamily includes yeast ubiquitin fusion degradation protein 2 (UFD2p) and its mammalian homolog, UBE4A. Yeast UFD2p, also known as ubiquitin conjugation factor E4 or UB fusion protein 2, is a polyubiquitin chain conjugation factor (E4) in the ubiquitin fusion degradation (UFD) pathway which catalyzes elongation of the ubiquitin chain through Lys48 linkage. It binds to substrates conjugated with one to three ubiquitin molecules and catalyzes the addition of further ubiquitin moieties in the presence of ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2) and ubiquitin ligase (E3), yielding multiubiquitylated substrates that are targets for the 26S proteasome. UFD2p is implicated in cell survival under stress conditions and is essential for homoeostasis of unsaturated fatty acids. It interacts with UBL-UBA proteins Rad23 and Dsk2, which are involved in the endoplasmic reticulum-associated degradation, ubiquitin fusion degradation, and OLE-1 gene induction pathways. UBE4A is a U-box-type ubiquitin-protein ligase that is located in common neuroblastoma deletion regions and may be subject to mutations in tumors. It may have a specific role in different biochemical processes other than ubiquitination, including growth or differentiation. Members of this family contain an N-terminal ubiquitin elongating factor core and a RING-like U-box domain at the C-terminus.


Pssm-ID: 438319  Cd Length: 70  Bit Score: 39.95  E-value: 1.28e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 53749680  15 PCISPVSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLID 54
Cdd:cd16657  15 PVILPSSKVTVDRSTIKRHLLSDQTDPFNRSPLTLDMVIP 54
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 297-327 3.06e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 38.06  E-value: 3.06e-04
                           10        20        30
                   ....*....|....*....|....*....|.
gi 53749680    297 SCAQVLRAHEGAVTGLSLHATGDYLLSCSDD 327
Cdd:smart00320   3 ELLKTLKGHTGPVTSVAFSPDGKYLASGSDD 33
WD40 pfam00400
WD domain, G-beta repeat;
297-327 4.17e-04

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 37.71  E-value: 4.17e-04
                          10        20        30
                  ....*....|....*....|....*....|.
gi 53749680   297 SCAQVLRAHEGAVTGLSLHATGDYLLSCSDD 327
Cdd:pfam00400   2 KLLKTLEGHTGSVTSLAFSPDGKLLASGSDD 32
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 354-378 1.33e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 36.14  E-value: 1.33e-03
                           10        20
                   ....*....|....*....|....*
gi 53749680    354 TCAQFHPDGLIFGTGTVDSQIKIWD 378
Cdd:smart00320  16 TSVAFSPDGKYLASGSDDGTIKLWD 40
mRING_PEX12 cd16451
Modified RING finger found in peroxin-12 (PEX12) and similar proteins; PEX12, also known as ...
 20-55 1.57e-03

Modified RING finger found in peroxin-12 (PEX12) and similar proteins; PEX12, also known as peroxisome assembly protein 12 or peroxisome assembly factor 3 (PAF-3), is a RING finger domain-containing integral membrane peroxin required for protein import into peroxisomes. Mutations in human PEX12 result in the peroxisome deficiency Zellweger syndrome of complementation group III (CG-III), a lethal neurological disorder. PEX12 also functions as an E3-ubiquitin ligase that facilitates the PEX4-dependent monoubiquitination of PEX5, a key player in peroxisomal matrix protein import, to control PEX5 receptor recycling or degradation. PEX12 contains a modified RING finger that lacks the third, fourth, and eighth zinc-binding residues of the consensus RING finger motif, suggesting PEX12 may only bind one zinc ion.


Pssm-ID: 438115 [Multi-domain]  Cd Length: 54  Bit Score: 36.45  E-value: 1.57e-03
                        10        20        30
                ....*....|....*....|....*....|....*.
gi 53749680  20 VSGHIFDRRLIEKYLAENGTDPVTNQPLSEDQLIDI 55
Cdd:cd16451  18 TSGYVFCYPCIYRYVKEHGRCPVTGYPASLDHLIKL 53
ANAPC4_WD40 pfam12894
Anaphase-promoting complex subunit 4 WD40 domain; Apc4 contains an N-terminal propeller-shaped ...
 339-403 2.39e-03

Anaphase-promoting complex subunit 4 WD40 domain; Apc4 contains an N-terminal propeller-shaped WD40 domain.The N-terminus of Afi1 serves to stabilize the union between Apc4 and Apc5, both of which lie towards the bottom-front of the APC,


Pssm-ID: 403945 [Multi-domain]  Cd Length: 91  Bit Score: 36.87  E-value: 2.39e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 53749680   339 RVLTKVTDESSGCAlTCAQFHPDGLIFGTGTVDSQIKIWDLKERSNVANFPGHSGPVSCIAFSEN 403
Cdd:pfam12894  28 RVWTLSPDKEDLEV-TSLAWRPDGKLLAVGYSDGTVRLLDAENGKIVHHFSAGSDLITCLGWGEN 91
SPL-RING_NSE2 cd16651
SPL-RING finger found in E3 SUMO-protein ligase NSE2 and similar proteins; NSE2, also known as ...
 3-62 3.86e-03

SPL-RING finger found in E3 SUMO-protein ligase NSE2 and similar proteins; NSE2, also known as MMS21 homolog (MMS21) or non-structural maintenance of chromosomes element 2 homolog (Non-SMC element 2 homolog, NSMCE2), is an autosumoylating small ubiquitin-like modifier (SUMO) ligase required for the response to DNA damage. It regulates sumoylation and nuclear-to-cytoplasmic translocation of skeletal and heart muscle-specific variant of the alpha subunit of nascent polypeptide associated complex (skNAC)-Smyd1 in myogenesis. It is also required for resisting extrinsically induced genotoxic stress. Moreover, NSE2 together with its partner proteins SMC6 and SMC5 form a tight subcomplex of the structural maintenance of chromosomes SMC5-6 complex, which includes another two subcomplexes, NSE1-NSE3-NSE4 and NSE5-NSE6. SMC6 and NSE3 are sumoylated in an NSE2-dependent manner, but SMC5 and NSE1 are not. NSE2-dependent E3 SUMO ligase activity is required for efficient DNA repair, but not for SMC5-6 complex stability. NSE2 contains a RING variant known as a Siz/PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like RING (SPL-RING) finger that is likely shared by the SP-RING type SUMO E3 ligases, such as PIAS family proteins. The SPL-RING finger is a variant of the RING finger, which lacks the second, fifth, and sixth zinc-binding residues of the consensus C3H2C3-/C3HC4-type RING fingers. It harbors only one Zn binding site and is required for the sumoylating activity.


Pssm-ID: 438313 [Multi-domain]  Cd Length: 67  Bit Score: 35.70  E-value: 3.86e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 53749680   3 LTCYISNEVTDRPCISPVSGHIFDRRLIEKYLAENGTD---PVT--NQPLSEDQLI-DIKVAHPIR 62
Cdd:cd16651   1 LKCPITQQLMVDPVRNKKCGHTYEKAAILQYLQSRKKKakcPVAgcRNTVSKSDLVpDPELKRRIE 66
WD40 pfam00400
WD domain, G-beta repeat;
352-378 8.08e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 34.24  E-value: 8.08e-03
                          10        20
                  ....*....|....*....|....*..
gi 53749680   352 ALTCAQFHPDGLIFGTGTVDSQIKIWD 378
Cdd:pfam00400  13 SVTSLAFSPDGKLLASGSDDGTVKVWD 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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