|
Name |
Accession |
Description |
Interval |
E-value |
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
199-712 |
0e+00 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 895.63 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 199 TQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALI 278
Cdd:cd05939 1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 279 YgedfleavtdvakdlpanltlfqfnnennnseteknipqakNLNALLTTASYEKPNKTQ-VNHHDKLVYIYTSGTTGLP 357
Cdd:cd05939 81 F-----------------------------------------NLLDPLLTQSSTEPPSQDdVNFRDKLFYIYTSGTTGLP 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 358 KAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQY 437
Cdd:cd05939 120 KAAVIVHSRYYRIAAGAYYAFGMRPEDVVYDCLPLYHSAGGIMGVGQALLHGSTVVIRKKFSASNFWDDCVKYNCTIVQY 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 438 IGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIAKVGEFYGATEGNANIMNHDNTVGAIGFVSRILPKI 517
Cdd:cd05939 200 IGEICRYLLAQPPSEEEQKHNVRLAVGNGLRPQIWEQFVRRFGIPQIGEFYGATEGNSSLVNIDNHVGACGFNSRILPSV 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 518 YPISIIRADPDTGEPIRDRNGLCQLCAPNEPGVFIGKIVKGNPSREFLGYVDEKASAKKIVKDVFKHGDMAFISGDLLVA 597
Cdd:cd05939 280 YPIRLIKVDEDTGELIRDSDGLCIPCQPGEPGLLVGKIIQNDPLRRFDGYVNEGATNKKIARDVFKKGDSAFLSGDVLVM 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 598 DEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVL 677
Cdd:cd05939 360 DELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVYGVEVPGVEGRAGMAAIVDPERKVDLDRFSAVLAKSL 439
|
490 500 510
....*....|....*....|....*....|....*
gi 939619614 678 PAYARPQIIRLLTKVDLTGTFKLRKVDLQKEGYDP 712
Cdd:cd05939 440 PPYARPQFIRLLPEVDKTGTFKLQKTDLQKEGYDP 474
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
153-746 |
0e+00 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 704.71 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 153 RDLTALWAYIKLLRYTKRHER-LNYTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVAL 231
Cdd:PRK08279 13 RRLPDLPGILRGLKRTALITPdSKRSLGDVFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVAL 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 232 LLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSE 311
Cdd:PRK08279 93 LMENRPEYLAAWLGLAKLGAVVALLNTQQRGAVLAHSLNLVDAKHLIVGEELVEAFEEARADLARPPRLWVAGGDTLDDP 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 312 TEknipqAKNLNALLTTASYEKPNKTQ-VNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPL 390
Cdd:PRK08279 173 EG-----YEDLAAAAAGAPTTNPASRSgVTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMGGFGGLLRLTPDDVLYCCL 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 391 PLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQ 470
Cdd:PRK08279 248 PLYHNTGGTVAWSSVLAAGATLALRRKFSASRFWDDVRRYRATAFQYIGELCRYLLNQPPKPTDRDHRLRLMIGNGLRPD 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 471 IWPQFVQRFNIAKVGEFYGATEGNANIMNHDNTVGAIGFVSRILPKiyPISIIRADPDTGEPIRDRNGLCQLCAPNEPGV 550
Cdd:PRK08279 328 IWDEFQQRFGIPRILEFYAASEGNVGFINVFNFDGTVGRVPLWLAH--PYAIVKYDVDTGEPVRDADGRCIKVKPGEVGL 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 551 FIGKIVKGNPsreFLGYVDEKASAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVS 630
Cdd:PRK08279 406 LIGRITDRGP---FDGYTDPEASEKKILRDVFKKGDAWFNTGDLMRDDGFGHAQFVDRLGDTFRWKGENVATTEVENALS 482
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 631 NVAGYKDTVVYGVTIPHTEGRAGMAAI-YDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQKEG 709
Cdd:PRK08279 483 GFPGVEEAVVYGVEVPGTDGRAGMAAIvLADGAEFDLAALAAHLYERLPAYAVPLFVRLVPELETTGTFKYRKVDLRKEG 562
|
570 580 590
....*....|....*....|....*....|....*...
gi 939619614 710 YDPNAIKDALYYQTSK-GRYELLTPQVYDQVQRNEIRF 746
Cdd:PRK08279 563 FDPSKVDDPLYVLDPGsGGYVPLTAELYAEIAAGKFRL 600
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
200-712 |
0e+00 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 586.24 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 200 QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIY 279
Cdd:cd05940 2 EALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 280 gedfleavtdvakdlpanltlfqfnnennnseteknipqaknlnallttasyekpnktqvnhhDKLVYIYTSGTTGLPKA 359
Cdd:cd05940 82 ---------------------------------------------------------------DAALYIYTSGTTGLPKA 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 360 AVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIG 439
Cdd:cd05940 99 AIISHRRAWRGGAFFAGSGGALPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRKKFSASNFWDDIRKYQATIFQYIG 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 440 EMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIAKVGEFYGATEGNANIMNHDNTVGAIGFVSRILPKIYP 519
Cdd:cd05940 179 ELCRYLLNQPPKPTERKHKVRMIFGNGLRPDIWEEFKERFGVPRIAEFYAATEGNSGFINFFGKPGAIGRNPSLLRKVAP 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 520 ISIIRADPDTGEPIRDRNGLCQLCAPNEPGVFIGKIvkgNPSREFLGYVDEKASAKKIVKDVFKHGDMAFISGDLLVADE 599
Cdd:cd05940 259 LALVKYDLESGEPIRDAEGRCIKVPRGEPGLLISRI---NPLEPFDGYTDPAATEKKILRDVFKKGDAWFNTGDLMRLDG 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 600 KGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYD-PERELDLDVFAASLAKVLP 678
Cdd:cd05940 336 EGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQVPGTDGRAGMAAIVLqPNEEFDLSALAAHLEKNLP 415
|
490 500 510
....*....|....*....|....*....|....
gi 939619614 679 AYARPQIIRLLTKVDLTGTFKLRKVDLQKEGYDP 712
Cdd:cd05940 416 GYARPLFLRLQPEMEITGTFKQQKVDLRNEGFDP 449
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
198-736 |
0e+00 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 547.66 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 198 ETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSA 276
Cdd:cd05938 2 EGETYTYRDVDRRSNQAARALLAHaGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAKV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 277 LIYGEDFLEAVTDVakdLPAnltLFQFNNENNNSETEKNIPQAKNLNALLTTASYEKPNK---TQVNHHDKLVYIYTSGT 353
Cdd:cd05938 82 LVVAPELQEAVEEV---LPA---LRADGVSVWYLSHTSNTEGVISLLDKVDAASDEPVPAslrAHVTIKSPALYIYTSGT 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 354 TGLPKAAVISHSRyLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNAT 433
Cdd:cd05938 156 TGLPKAARISHLR-VLQCSGFLSLCGVTADDVIYITLPLYHSSGFLLGIGGCIELGATCVLKPKFSASQFWDDCRKHNVT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 434 IGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIAKVGEFYGATEGNANIMNHDNTVGAIGFVSRI 513
Cdd:cd05938 235 VIQYIGELLRYLCNQPQSPNDRDHKVRLAIGNGLRADVWREFLRRFGPIRIREFYGSTEGNIGFFNYTGKIGAVGRVSYL 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 514 LPKIYPISIIRADPDTGEPIRDRNGLCQLCAPNEPGVFIGKIVKGNPsreFLGYV-DEKASAKKIVKDVFKHGDMAFISG 592
Cdd:cd05938 315 YKLLFPFELIKFDVEKEEPVRDAQGFCIPVAKGEPGLLVAKITQQSP---FLGYAgDKEQTEKKLLRDVFKKGDVYFNTG 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 593 DLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAI-YDPERELDLDVFAA 671
Cdd:cd05938 392 DLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLLDFLQEVNVYGVTVPGHEGRIGMAAVkLKPGHEFDGKKLYQ 471
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 672 SLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQKEGYDPNAIKDALYY-QTSKGRYELLTPQVY 736
Cdd:cd05938 472 HVREYLPAYARPRFLRIQDSLEITGTFKQQKVRLVEEGFNPSIISDPLYFlDETQKTYVPLTPDIY 537
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
202-712 |
4.00e-148 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 440.72 E-value: 4.00e-148
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 202 WTFRQVNEHANKVANVLQ-AQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYG 280
Cdd:cd05937 6 WTYSETYDLVLRYAHWLHdDLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRFVIVD 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 281 EDFLeavtdvakdlpanltlfqfnnennnseteknipqaknlnALLttasyekpnktqvnhhdklvyIYTSGTTGLPKAA 360
Cdd:cd05937 86 PDDP---------------------------------------AIL---------------------IYTSGTTGLPKAA 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 361 VISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGE 440
Cdd:cd05937 106 AISWRRTLVTSNLLSHDLNLKNGDRTYTCMPLYHGTAAFLGACNCLMSGGTLALSRKFSASQFWKDVRDSGATIIQYVGE 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 441 MARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIAKVGEFYGATEGNANIMNH---DNTVGAIGFVSRILPKI 517
Cdd:cd05937 186 LCRYLLSTPPSPYDRDHKVRVAWGNGLRPDIWERFRERFNVPEIGEFYAATEGVFALTNHnvgDFGAGAIGHHGLIRRWK 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 518 Y--PISIIRADPDTGEPIRD-RNGLCQLCAPNEPGVFIGKIvKGNPSREFLGYV-DEKASAKKIVKDVFKHGDMAFISGD 593
Cdd:cd05937 266 FenQVVLVKMDPETDDPIRDpKTGFCVRAPVGEPGEMLGRV-PFKNREAFQGYLhNEDATESKLVRDVFRKGDIYFRTGD 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 594 LLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYDPERELDLDVFA--- 670
Cdd:cd05937 345 LLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEANVYGVKVPGHDGRAGCAAITLEESSAVPTEFTksl 424
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 939619614 671 -ASLAKV-LPAYARPQIIRLLTKVDLTGTFKLRKVDLQKEGYDP 712
Cdd:cd05937 425 lASLARKnLPSYAVPLFLRLTEEVATTDNHKQQKGVLRDEGVDP 468
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
200-706 |
6.17e-89 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 285.72 E-value: 6.17e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 200 QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALiy 279
Cdd:cd05934 2 RRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLV-- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 280 gedfleaVTDvakdlPANLtlfqfnnennnseteknipqaknlnallttasyekpnktqvnhhdklvyIYTSGTTGLPKA 359
Cdd:cd05934 80 -------VVD-----PASI-------------------------------------------------LYTSGTTGPPKG 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 360 AVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIG 439
Cdd:cd05934 99 VVITHANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVLLPRFSASRFWSDVRRYGATVTNYLG 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 440 EMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIaKVGEFYGATEGNANIMN---HDNTVGAIGfvsrilpk 516
Cdd:cd05934 179 AMLSYLLAQPPSPDDRAHRLRAAYGAPNPPELHEEFEERFGV-RLLEGYGMTETIVGVIGprdEPRRPGSIG-------- 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 517 iypisiiRADPDTGEPIRDRNGlcQLCAPNEPGVFIGKIVKGnPSReFLGYV-DEKASAKkivkdVFKHGdmAFISGDLL 595
Cdd:cd05934 250 -------RPAPGYEVRIVDDDG--QELPAGEPGELVIRGLRG-WGF-FKGYYnMPEATAE-----AMRNG--WFHTGDLG 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 596 VADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYDPERELDLDVFAASLAK 675
Cdd:cd05934 312 YRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPEELFAFCEG 391
|
490 500 510
....*....|....*....|....*....|.
gi 939619614 676 VLPAYARPQIIRLLTKVDLTGTFKLRKVDLQ 706
Cdd:cd05934 392 QLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
178-708 |
9.39e-83 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 270.53 E-value: 9.39e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 178 VADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLIN 257
Cdd:COG0318 1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 258 TNLRGPSLLHSITVAHCSALIYgedfleavtdvakdlpanltlfqfnnennnseteknipqaknlnALLttasyekpnkt 337
Cdd:COG0318 81 PRLTAEELAYILEDSGARALVT--------------------------------------------ALI----------- 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 338 qvnhhdklvyIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKK 417
Cdd:COG0318 106 ----------LYTSGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLAGATLVLLPR 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 418 FSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVF--GNGLRPQIWPQFVQRFNIAkVGEFYGATEGNA 495
Cdd:COG0318 176 FDPERVLELIERERVTVLFGVPTMLARLLRHPEFARYDLSSLRLVVsgGAPLPPELLERFEERFGVR-IVEGYGLTETSP 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 496 NIM-----NHDNTVGAIGfvsrilpKIYPISIIRadpdtgepIRDRNGlcQLCAPNEPGVFigkIVKGnPSReFLGYV-D 569
Cdd:COG0318 255 VVTvnpedPGERRPGSVG-------RPLPGVEVR--------IVDEDG--RELPPGEVGEI---VVRG-PNV-MKGYWnD 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 570 EKASAKkivkdVFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTe 649
Cdd:COG0318 313 PEATAE-----AFRDG--WLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKW- 384
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 650 GRAGMAAI-YDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQKE 708
Cdd:COG0318 385 GERVVAFVvLRPGAELDAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRER 444
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
177-713 |
1.32e-66 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 230.03 E-value: 1.32e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLI 256
Cdd:PRK06155 22 TLPAMLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDV-AKDLP-ANLTLFQFNNENNNSETEKNIPQAKnLNALLTTASyekp 334
Cdd:PRK06155 102 NTALRGPQLEHILRNSGARLLVVEAALLAALEAAdPGDLPlPAVWLLDAPASVSVPAGWSTAPLPP-LDAPAPAAA---- 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 nktqVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTaGGIMCMGQSVLFGSTVSI 414
Cdd:PRK06155 177 ----VQPGDTAAILYTSGTTGPSKGVCCPHAQFYWWGRNSAEDLEIGADDVLYTTLPLFHT-NALNAFFQALLAGATYVL 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 RKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIAKVgEFYGATEGN 494
Cdd:PRK06155 252 EPRFSASGFWPAVRRHGATVTYLLGAMVSILLSQPARESDRAHRVRVALGPGVPAALHAAFRERFGVDLL-DGYGSTETN 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 495 ANI-MNHDNTVGaiGFVSRILPKIypisIIRadpdtgepIRDRNGlcQLCAPNEPGVFigkIVKGNPSREFL-GYVDEKA 572
Cdd:PRK06155 331 FVIaVTHGSQRP--GSMGRLAPGF----EAR--------VVDEHD--QELPDGEPGEL---LLRADEPFAFAtGYFGMPE 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 573 SAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRA 652
Cdd:PRK06155 392 KTVEAWRNLWFH------TGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEV 465
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 653 GMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQKEGYDPN 713
Cdd:PRK06155 466 MAAVVLRDGTALEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQGVTAD 526
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
182-616 |
6.37e-63 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 216.41 E-value: 6.37e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 182 FERNVQAHPDKVAV-VSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNL 260
Cdd:pfam00501 1 LERQAARTPDKTALeVGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 261 RGPSLLHSITVAHCSALIygedfleavTDVAKDLPANLTLFQFNNENNNSETEKNIPQAKNLNALLTTASYEKPNKTQV- 339
Cdd:pfam00501 81 PAEELAYILEDSGAKVLI---------TDDALKLEELLEALGKLEVVKLVLVLDRDPVLKEEPLPEEAKPADVPPPPPPp 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 340 NHHDKLVYI-YTSGTTGLPKAAVISH----SRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSI 414
Cdd:pfam00501 152 PDPDDLAYIiYTSGTTGKPKGVMLTHrnlvANVLSIKRVRPRGFGLGPDDRVLSTLPLFHDFGLSLGLLGPLLAGATVVL 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 RKKFSA---SNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVF--GNGLRPQIWPQFVQRFNIAkVGEFYG 489
Cdd:pfam00501 232 PPGFPAldpAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSSLRLVLsgGAPLPPELARRFRELFGGA-LVNGYG 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 490 ATEG------NANIMNHDNTVGAIGfvsRILP----KIypisiirADPDTGEPirdrnglcqlCAPNEPG--VFIGKIVk 557
Cdd:pfam00501 311 LTETtgvvttPLPLDEDLRSLGSVG---RPLPgtevKI-------VDDETGEP----------VPPGEPGelCVRGPGV- 369
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 558 gnpsreFLGYV-DEKASAKkivkdVFKHGDMaFISGDLLVADEKGYLYFKDRTGDTFRWK 616
Cdd:pfam00501 370 ------MKGYLnDPELTAE-----AFDEDGW-YRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
177-734 |
6.91e-62 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 216.82 E-value: 6.91e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVAD-VFERnvqAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDV-VALLLENRAEYVaTWLGLSKIGVITP 254
Cdd:PRK13388 4 TIAQlLRDR---AGDDTIAVRYGDRTWTWREVLAEAAARAAALIALADPDRPLhVGVLLGNTPEML-FWLAAAALGGYVL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 L-INTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVakDLPANLTLfqfnnennnsetEKNIPQAKNLnalLTTASYEK 333
Cdd:PRK13388 80 VgLNTTRRGAALAADIRRADCQLLVTDAEHRPLLDGL--DLPGVRVL------------DVDTPAYAEL---VAAAGALT 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 334 PNkTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVS 413
Cdd:PRK13388 143 PH-REVDAMDPFMLIFTSGTTGAPKAVRCSHGRLAFAGRALTERFGLTRDDVCYVSMPLFHSNAVMAGWAPAVASGAAVA 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 414 IRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNiAKVGEFYGATEG 493
Cdd:PRK13388 222 LPAKFSASGFLDDVRRYGATYFNYVGKPLAYILATPERPDDADNPLRVAFGNEASPRDIAEFSRRFG-CQVEDGYGSSEG 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 494 NANIMNHDNT-VGAIGfvsrilpKIYP-ISIIraDPDTGEP----IRDRNGlcQLCAPNEPgvfIGKIVKGNPSREFLGY 567
Cdd:PRK13388 301 AVIVVREPGTpPGSIG-------RGAPgVAIY--NPETLTEcavaRFDAHG--ALLNADEA---IGELVNTAGAGFFEGY 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 568 V-DEKASAKKIvkdvfKHGDmaFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIP 646
Cdd:PRK13388 367 YnNPEATAERM-----RHGM--YWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDE 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 647 HTeGRAGMAAI-YDPERELDLDVFAASLAKV--LPAYARPQIIRLLTKVDLTGTFKLRKVDLQKEGYDPNAiKDALYYQT 723
Cdd:PRK13388 440 RV-GDQVMAALvLRDGATFDPDAFAAFLAAQpdLGTKAWPRYVRIAADLPSTATNKVLKRELIAQGWATGD-PVTLWVRR 517
|
570
....*....|.
gi 939619614 724 SKGRYELLTPQ 734
Cdd:PRK13388 518 GGPAYRLMSEP 528
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
177-711 |
4.52e-58 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 206.07 E-value: 4.52e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERnvQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDV-VALLLENRAEYvATWLGLSKIGVITPL 255
Cdd:PRK07867 6 TVAELLLP--LAEDDDRGLYFEDSFTSWREHIRGSAARAAALRARLDPTRPPhVGVLLDNTPEF-SLLLGAAALSGIVPV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 -INTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTlfqfnnennnseteknipQAKNLNALLTTASYEKP 334
Cdd:PRK07867 83 gLNPTRRGAALARDIAHADCQLVLTESAHAELLDGLDPGVRVINV------------------DSPAWADELAAHRDAEP 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 NKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSI 414
Cdd:PRK07867 145 PFRVADPDDLFMLIFTSGTSGDPKAVRCTHRKVASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVALAAGASIAL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 RKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNiAKVGEFYGATEGN 494
Cdd:PRK07867 225 RRKFSASGFLPDVRRYGATYANYVGKPLSYVLATPERPDDADNPLRIVYGNEGAPGDIARFARRFG-CVVVDGFGSTEGG 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 495 ANIMNHDNT-VGAIGfvsrilPKIYPISIIraDPDTGEPirdrnglcqlCAPNEPGV--------FIGKIVKGNPSREFL 565
Cdd:PRK07867 304 VAITRTPDTpPGALG------PLPPGVAIV--DPDTGTE----------CPPAEDADgrllnadeAIGELVNTAGPGGFE 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 566 GYV-DEKASAKKIVKDVFkHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVT 644
Cdd:PRK07867 366 GYYnDPEADAERMRGGVY-W------SGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVP 438
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 645 IPHTeGRAGMAAI-YDPERELDLDVFAASLAKV--LPAYARPQIIRLLTKVDLTGTFKLRKVDLQKEGYD 711
Cdd:PRK07867 439 DPVV-GDQVMAALvLAPGAKFDPDAFAEFLAAQpdLGPKQWPSYVRVCAELPRTATFKVLKRQLSAEGVD 507
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
345-701 |
4.75e-53 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 186.72 E-value: 4.75e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 345 LVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTaGGIMCMGQSVLFGSTVSIRKKFSASNYF 424
Cdd:cd04433 3 ALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPKFDPEAAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 425 ADCAKYNATIGQYIGEMARYIL-ATKPSEYDQkHRVRLVFGNG--LRPQIWPQFVQRFNIaKVGEFYGATEGN--ANIMN 499
Cdd:cd04433 82 ELIEREKVTILLGVPTLLARLLkAPESAGYDL-SSLRALVSGGapLPPELLERFEEAPGI-KLVNGYGLTETGgtVATGP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 500 HDNTVGAIGFVSRILPKIYpISIIraDPDTGEpirdrnglcqlCAPNEpgvfIGKIVKGNPSReFLGYV-DEKASAKKIV 578
Cdd:cd04433 160 PDDDARKPGSVGRPVPGVE-VRIV--DPDGGE-----------LPPGE----IGELVVRGPSV-MKGYWnNPEATAAVDE 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 579 KDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPHTE-GRAGMAAI 657
Cdd:cd04433 221 DGWYRTGDLGRL-------DEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGV--PDPEwGERVVAVV 291
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 939619614 658 -YDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLR 701
Cdd:cd04433 292 vLRPGADLDAEELRAHVRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
177-710 |
1.53e-49 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 182.31 E-value: 1.53e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLI 256
Cdd:PRK06187 7 TIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPI 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHsiTVAHCSA--LIYGEDFLEAVTDVAKDLPanltLFQFNNENNNSETEKNIPQAKNLNALLTTASYEKP 334
Cdd:PRK06187 87 NIRLKPEEIAY--ILNDAEDrvVLVDSEFVPLLAAILPQLP----TVRTVIVEGDGPAAPLAPEVGEYEELLAAASDTFD 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 NKtQVNHHDKLVYIYTSGTTGLPKAAVISHsRYLFI-AAGIHYTMGFQEEDIFYTPLPLYHtAGGIMCMGQSVLFGSTVS 413
Cdd:PRK06187 161 FP-DIDENDAAAMLYTSGTTGHPKGVVLSH-RNLFLhSLAVCAWLKLSRDDVYLVIVPMFH-VHAWGLPYLALMAGAKQV 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 414 IRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVF--GNGLRPQIWPQFVQRFNIaKVGEFYGAT 491
Cdd:PRK06187 238 IPRRFDPENLLDLIETERVTFFFAVPTIWQMLLKAPRAYFVDFSSLRLVIygGAALPPALLREFKEKFGI-DLVQGYGMT 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 492 E-GNANIMNH--DNTVGAIGF---VSRILPkIYPISIIRAD-----PDTGEpirdrnglcqlcapnepgvfIGKI-VKGn 559
Cdd:PRK06187 317 EtSPVVSVLPpeDQLPGQWTKrrsAGRPLP-GVEARIVDDDgdelpPDGGE--------------------VGEIiVRG- 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 560 PSReFLGYV-DEKASAKKIVKDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDT 638
Cdd:PRK06187 375 PWL-MQGYWnRPEATAETIDGGWLHTGDVGYI-------DEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEV 446
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 939619614 639 VVYGVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLqKEGY 710
Cdd:PRK06187 447 AVIGVPDEKWGERPVAVVVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVL-REQY 517
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
182-702 |
9.30e-46 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 169.33 E-value: 9.30e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 182 FERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLR 261
Cdd:cd17631 1 LRRRARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLT 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 262 GPSLLHSitVAHCSAliygedfleavtdvakdlpanLTLFqfnnennnseteknipqaknlnallttasyekpnktqvnh 341
Cdd:cd17631 81 PPEVAYI--LADSGA---------------------KVLF---------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 342 HDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSAS 421
Cdd:cd17631 98 DDLALLMYTSGTTGRPKGAMLTHRNLLWNAVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTLLRGGTVVILRKFDPE 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 422 NYFADCAKYNATIGQYIGEMArYILATKP--SEYDQKhRVRLVFGNG------LRPQIW---PQFVQrfniakvgeFYGA 490
Cdd:cd17631 178 TVLDLIERHRVTSFFLVPTMI-QALLQHPrfATTDLS-SLRAVIYGGapmperLLRALQargVKFVQ---------GYGM 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 491 TE--GNANIMNHDNTVGAIGFVSRILPKIyPISIIraDPDTGEpirdrnglcqlCAPNEpgvfIGKIVKGNPSReFLGYV 568
Cdd:cd17631 247 TEtsPGVTFLSPEDHRRKLGSAGRPVFFV-EVRIV--DPDGRE-----------VPPGE----VGEIVVRGPHV-MAGYW 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 569 D-EKASAKKIVKDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPH 647
Cdd:cd17631 308 NrPEATAAAFRDGWFHTGDLGRL-------DEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGV--PD 378
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 939619614 648 TE-GRAGMAAIY-DPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRK 702
Cdd:cd17631 379 EKwGEAVVAVVVpRPGAELDEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
172-717 |
1.26e-44 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 169.14 E-value: 1.26e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 172 ERLNYtVADVFERNVQAHPDKVAVVSE-----TQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGL 246
Cdd:COG0365 6 GRLNI-AYNCLDRHAEGRGDKVALIWEgedgeERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLAC 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 247 SKIGVITPLINTNLRGPSLLHSITVAHCSALI------YGE---DFLEAVTDVAKDLPANLTLFQFnnenNNSETEKNIP 317
Cdd:COG0365 85 ARIGAVHSPVFPGFGAEALADRIEDAEAKVLItadgglRGGkviDLKEKVDEALEELPSLEHVIVV----GRTGADVPME 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 318 QAKNLNALLTTASyEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIH-YTMGFQEEDIFYTplplyhTA 396
Cdd:COG0365 161 GDLDWDELLAAAS-AEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAkYVLDLKPGDVFWC------TA 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 397 --GGIMCMGQSV----LFGSTVSI---RKKF-SASNYFADCAKYNATI----GQYIGEMARYILAtKPSEYDQKHrVRLV 462
Cdd:COG0365 234 diGWATGHSYIVygplLNGATVVLyegRPDFpDPGRLWELIEKYGVTVfftaPTAIRALMKAGDE-PLKKYDLSS-LRLL 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 463 FGNG--LRPQIWPQFVQRFNIaKVGEFYGATEgnanimnhdnTVGAIGFVSRILPkIYPISI------IRADpdtgepIR 534
Cdd:COG0365 312 GSAGepLNPEVWEWWYEAVGV-PIVDGWGQTE----------TGGIFISNLPGLP-VKPGSMgkpvpgYDVA------VV 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 535 DRNGlcQLCAPNEPGVFIgkIVKGNPS--REFLGyvDEKASAKK---IVKDVFKHGDMAFIsgdllvaDEKGYLYFKDRT 609
Cdd:COG0365 374 DEDG--NPVPPGEEGELV--IKGPWPGmfRGYWN--DPERYRETyfgRFPGWYRTGDGARR-------DEDGYFWILGRS 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 610 GDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPHTEGRAGMAAI------YDPERELDLDVFAAsLAKVLPAYARP 683
Cdd:COG0365 441 DDVINVSGHRIGTAEIESALVSHPAVAEAAVVGV--PDEIRGQVVKAFvvlkpgVEPSDELAKELQAH-VREELGPYAYP 517
|
570 580 590
....*....|....*....|....*....|....*....
gi 939619614 684 QIIRLLTKVDLTGT-----FKLRKVDLQKEGYDPNAIKD 717
Cdd:COG0365 518 REIEFVDELPKTRSgkimrRLLRKIAEGRPLGDTSTLED 556
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
175-643 |
6.12e-44 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 166.01 E-value: 6.12e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 175 NYTVADVFERNVQAHPDKVAVVSE-----TQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKI 249
Cdd:PRK08008 6 GQHLRQMWDDLADVYGHKTALIFEssggvVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKI 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 250 GVITPLINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTlfqfnNENNNSETEKNIPQAKNLNALLTTA 329
Cdd:PRK08008 86 GAIMVPINARLLREESAWILQNSQASLLVTSAQFYPMYRQIQQEDATPLR-----HICLTRVALPADDGVSSFTQLKAQQ 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 330 SYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFiaAGiHYT---MGFQEEDIFYTPLPLYHTagGIMCMGQSV 406
Cdd:PRK08008 161 PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRF--AG-YYSawqCALRDDDVYLTVMPAFHI--DCQCTAAMA 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 407 LF--GSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVR-LVFGNGLRPQIWPQFVQRFNIaK 483
Cdd:PRK08008 236 AFsaGATFVLLEKYSARAFWGQVCKYRATITECIPMMIRTLMVQPPSANDRQHCLReVMFYLNLSDQEKDAFEERFGV-R 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 484 VGEFYGATEgnanimnhdNTVGAIG---FVSRILPkiypiSIIRADPDTGEPIRDRNGlcQLCAPNEpgvfIGKI-VKGN 559
Cdd:PRK08008 315 LLTSYGMTE---------TIVGIIGdrpGDKRRWP-----SIGRPGFCYEAEIRDDHN--RPLPAGE----IGEIcIKGV 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 560 PSREFLG--YVDEKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKD 637
Cdd:PRK08008 375 PGKTIFKeyYLDPKATAKVLEADGWLH------TGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQD 448
|
....*.
gi 939619614 638 TVVYGV 643
Cdd:PRK08008 449 IVVVGI 454
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
178-702 |
2.31e-43 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 163.50 E-value: 2.31e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 178 VADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLIN 257
Cdd:cd05936 1 LADLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 258 TNLRGPSLLHSITVAHCSALIYGEDFLEAVtdvakdlpanltlfqfnnennnseteknipqaknlnallttASYEKPNKT 337
Cdd:cd05936 81 PLYTPRELEHILNDSGAKALIVAVSFTDLL-----------------------------------------AAGAPLGER 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 338 QVNH-HDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEE--DIFYTPLPLYHTAGGIMCMGQSVLFGSTVSI 414
Cdd:cd05936 120 VALTpEDVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWLEDLLEgdDVVLAALPLFHVFGLTVALLLPLALGATIVL 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 RKKFSASNYFADCAKYNATIgqYIG--EMARYILATKPSEYDQKHRVRLVFGNG--LRPQIWPQFVQRFNiAKVGEFYGA 490
Cdd:cd05936 200 IPRFRPIGVLKEIRKHRVTI--FPGvpTMYIALLNAPEFKKRDFSSLRLCISGGapLPVEVAERFEELTG-VPIVEGYGL 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 491 TE----GNANIMNHDNTVGAIGfvsrilpkiYPIsiiradPDTGEPIRDRNGlcQLCAPNEPGVFigkIVKGnPSReFLG 566
Cdd:cd05936 277 TEtspvVAVNPLDGPRKPGSIG---------IPL------PGTEVKIVDDDG--EELPPGEVGEL---WVRG-PQV-MKG 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 567 YV-DEKASAKKIVKDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTI 645
Cdd:cd05936 335 YWnRPEETAEAFVDGWLRTGDIGYM-------DEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPD 407
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 939619614 646 PHTeGRAGMAAIYDPERE-LDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRK 702
Cdd:cd05936 408 PYS-GEAVKAFVVLKEGAsLTEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILR 464
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
197-643 |
3.78e-43 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 163.15 E-value: 3.78e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 197 SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSA 276
Cdd:cd05911 6 DTGKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKV 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 277 LIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSETEKNIPQAKnlnaLLTTASYEKPNKtqVNHHDKLVYI-YTSGTTG 355
Cdd:cd05911 86 IFTDPDGLEKVKEAAKELGPKDKIIVLDDKPDGVLSIEDLLSPT----LGEEDEDLPPPL--KDGKDDTAAIlYSSGTTG 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 356 LPKAAVISHSRylFIAAGIHYTMGFQE----EDIFYTPLPLYHTAGGIMCMGqSVLFGSTVSIRKKFSASNYFADCAKYN 431
Cdd:cd05911 160 LPKGVCLSHRN--LIANLSQVQTFLYGndgsNDVILGFLPLYHIYGLFTTLA-SLLNGATVIIMPKFDSELFLDLIEKYK 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 432 ATIGQYIGEMArYILATKPS--EYDQKHRVRLVFGNG-LRPQIWPQFVQRFNIAKVGEFYGATE-GNANIMN--HDNTVG 505
Cdd:cd05911 237 ITFLYLVPPIA-AALAKSPLldKYDLSSLRVILSGGApLSKELQELLAKRFPNATIKQGYGMTEtGGILTVNpdGDDKPG 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 506 AIGfvsRILP----KIypisiirADPDTGepirdrnglcQLCAPNEPGVFigkIVKGnPSReFLGYV-DEKASAKKIVKD 580
Cdd:cd05911 316 SVG---RLLPnveaKI-------VDDDGK----------DSLGPNEPGEI---CVRG-PQV-MKGYYnNPEATKETFDED 370
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 939619614 581 VFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGV 643
Cdd:cd05911 371 GWLH------TGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGI 427
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
202-707 |
1.11e-38 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 149.02 E-value: 1.11e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 202 WTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYGE 281
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTDA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 282 DfleavtdvakdlpanltlfqfnnennnseteknipqaknlnallttasyekpnktqvnhhDKLVYIYTSGTTGLPKAAV 361
Cdd:cd05972 81 E------------------------------------------------------------DPALIYFTSGTTGLPKGVL 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 362 ISHSRYLFIAAGIHYTMGFQEEDIFYTP------LPLYHTAGGIMCMGQSVLfgstVSIRKKFSASNYFADCAKYNATIG 435
Cdd:cd05972 101 HTHSYPLGHIPTAAYWLGLRPDDIHWNIadpgwaKGAWSSFFGPWLLGATVF----VYEGPRFDAERILELLERYGVTSF 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 436 QYIGEMARYILATKPSEYDQKHrVRLVFGNG--LRPQIWPQFVQRFNIaKVGEFYGATEGNANIMNHDNTvgaigfvsri 513
Cdd:cd05972 177 CGPPTAYRMLIKQDLSSYKFSH-LRLVVSAGepLNPEVIEWWRAATGL-PIRDGYGQTETGLTVGNFPDM---------- 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 514 lpKIYPISIIRADPDTGEPIRDRNGlcQLCAPNEPGVFigkIVKGNPSREFLGYV-DEKASAKKIVKDVFKHGDMAfisg 592
Cdd:cd05972 245 --PVKPGSMGRPTPGYDVAIIDDDG--RELPPGEEGDI---AIKLPPPGLFLGYVgDPEKTEASIRGDYYLTGDRA---- 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 593 dllVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPHTEgRAGM-------AAIYDPERELD 665
Cdd:cd05972 314 ---YRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGS--PDPV-RGEVvkafvvlTSGYEPSEELA 387
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 939619614 666 LDvFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQK 707
Cdd:cd05972 388 EE-LQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
177-647 |
2.54e-38 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 149.70 E-value: 2.54e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLI 256
Cdd:PRK08316 12 TIGDILRRSARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSETeknipQAKNLNALLTTASYEKPNk 336
Cdd:PRK08316 92 NFMLTGEELAYILDHSGARAFLVDPALAPTAEAALALLPVDTLILSLVLGGREAPG-----GWLDFADWAEAGSVAEPD- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 337 TQVNHHDKLVYIYTSGTTGLPKAAVISH----SRYL-FIAAGihytmGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGST 411
Cdd:PRK08316 166 VELADDDLAQILYTSGTESLPKGAMLTHraliAEYVsCIVAG-----DMSADDIPLHALPLYHCAQLDVFLGPYLYVGAT 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 412 VSIRKKFSASNYFADCAKYNAT---------IGqyigemaryiLATKPsEYDqKHRVRlvfgnGLR-----PQIWPQFV- 476
Cdd:PRK08316 241 NVILDAPDPELILRTIEAERITsffapptvwIS----------LLRHP-DFD-TRDLS-----SLRkgyygASIMPVEVl 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 477 ----QRFNIAKVGEFYGATEGN--ANIMNHDNTVGAIG-------FV-SRILpkiypisiiraDPDtGEPIrdrnglcql 542
Cdd:PRK08316 304 kelrERLPGLRFYNCYGQTEIAplATVLGPEEHLRRPGsagrpvlNVeTRVV-----------DDD-GNDV--------- 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 543 cAPNEPgvfiGKIVKGNPsREFLGYVD--EKASAkkivkdVFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENV 620
Cdd:PRK08316 363 -APGEV----GEIVHRSP-QLMLGYWDdpEKTAE------AFRGG--WFHSGDLGVMDEEGYITVVDRKKDMIKTGGENV 428
|
490 500 510
....*....|....*....|....*....|...
gi 939619614 621 STSEVE------AQVSNVAgykdtvVYGVtiPH 647
Cdd:PRK08316 429 ASREVEealythPAVAEVA------VIGL--PD 453
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
177-643 |
1.74e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 138.91 E-value: 1.74e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLI 256
Cdd:PRK07788 50 PFAGLVAHAARRAPDRAALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILL 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLfqfnnENNNSETEKNIPQAKNLNALLTTASYEKPNK 336
Cdd:PRK07788 130 NTGFSGPQLAEVAAREGVKALVYDDEFTDLLSALPPDLGRLRAW-----GGNPDDDEPSGSTDETLDDLIAGSSTAPLPK 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 337 tqVNHHDKLVyIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAgGIMCMGQSVLFGSTVSIRK 416
Cdd:PRK07788 205 --PPKPGGIV-ILTSGTTGTPKGAPRPEPSPLAPLAGLLSRVPFRAGETTLLPAPMFHAT-GWAHLTLAMALGSTVVLRR 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 417 KFSASNYFADCAKYNATIGQYIGEMARYILATKP---SEYDQKhRVRLVFGNGlrPQIWPQFVQRFNiAKVGE----FYG 489
Cdd:PRK07788 281 RFDPEATLEDIAKHKATALVVVPVMLSRILDLGPevlAKYDTS-SLKIIFVSG--SALSPELATRAL-EAFGPvlynLYG 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 490 ATE-GNANIMNHDNTVGAIGFVSRILP----KIYpisiiraDPDtGEPIrdrnglcqlcapnEPGVfIGKIVKGNpSREF 564
Cdd:PRK07788 357 STEvAFATIATPEDLAEAPGTVGRPPKgvtvKIL-------DEN-GNEV-------------PRGV-VGRIFVGN-GFPF 413
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 939619614 565 LGYVDEKasAKKIVKDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGV 643
Cdd:PRK07788 414 EGYTDGR--DKQIIDGLLSSGDVGYF-------DEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGV 483
|
|
| PRK07868 |
PRK07868 |
acyl-CoA synthetase; Validated |
187-740 |
3.89e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236121 [Multi-domain] Cd Length: 994 Bit Score: 140.24 E-value: 3.89e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 187 QAH--PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTnlrGPS 264
Cdd:PRK07868 456 QARdaPKGEFLLFDGRVHTYEAVNRRINNVVRGLIAVGVRQGDRVGVLMETRPSALVAIAALSRLGAVAVLMPP---DTD 532
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 265 LLHSITVAHCSALIYGEDFLEAVTDVAK-------------DLPANLTLFQFNNENnnsetekniPQAKNLNAllttasY 331
Cdd:PRK07868 533 LAAAVRLGGVTEIITDPTNLEAARQLPGrvlvlgggesrdlDLPDDADVIDMEKID---------PDAVELPG------W 597
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 332 EKPNKTQVNhhdKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGST 411
Cdd:PRK07868 598 YRPNPGLAR---DLAFIAFSTAGGELVAKQITNYRWALSAFGTASAAALDRRDTVYCLTPLHHESGLLVSLGGAVVGGSR 674
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 412 VSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIAKVGEFYGAT 491
Cdd:PRK07868 675 IALSRGLDPDRFVQEVRQYGVTVVSYTWAMLREVVDDPAFVLHGNHPVRLFIGSGMPTGLWERVVEAFAPAHVVEFFATT 754
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 492 EGNANIMNhdntVGA--IGFVSRILPKIYPISIIRADPDTGEPIRDRNGLCQLCAPNEPGVFIGKivkgnpSReflGYVD 569
Cdd:PRK07868 755 DGQAVLAN----VSGakIGSKGRPLPGAGRVELAAYDPEHDLILEDDRGFVRRAEVNEVGVLLAR------AR---GPID 821
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 570 EKASAKkivKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTE 649
Cdd:PRK07868 822 PTASVK---RGVFAPADTWISTEYLFRRDDDGDYWLVDRRGSVIRTARGPVYTEPVTDALGRIGGVDLAVTYGVEVGGRQ 898
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 650 gRAGMAAIYDPERELDLDVFAASLAKvLPAYARPQIIRLLTKVDLTGTFKLRKVDLQKEGYdPNAIKDALYYQTSKGRYE 729
Cdd:PRK07868 899 -LAVAAVTLRPGAAITAADLTEALAS-LPVGLGPDIVHVVPEIPLSATYRPTVSALRAAGI-PKPGRQAWYFDPETNRYR 975
|
570
....*....|.
gi 939619614 730 LLTPQVYDQVQ 740
Cdd:PRK07868 976 RLTPAVRAELT 986
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
186-644 |
1.37e-31 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 129.31 E-value: 1.37e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 186 VQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSL 265
Cdd:PRK03640 12 AFLTPDRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREEL 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 266 LHSITVAHCSALIYGEDFLEAVTdvakdlpanltlfqfnnennnseteknIPQAKNLNALLTTAsYEKPNKTQVNHHDKL 345
Cdd:PRK03640 92 LWQLDDAEVKCLITDDDFEAKLI---------------------------PGISVKFAELMNGP-KEEAEIQEEFDLDEV 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 346 VYI-YTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTaGGIMCMGQSVLFGSTVSIRKKFSASNYF 424
Cdd:PRK03640 144 ATImYTSGTTGKPKGVIQTYGNHWWSAVGSALNLGLTEDDCWLAAVPIFHI-SGLSILMRSVIYGMRVVLVEKFDAEKIN 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 425 ADCAKYNATIGQYIGEMARYILATKPSE-YDQKHRVRLVfGNGLRPQIWPQFVQRFNIAkVGEFYGATEGNANI--MNHD 501
Cdd:PRK03640 223 KLLQTGGVTIISVVSTMLQRLLERLGEGtYPSSFRCMLL-GGGPAPKPLLEQCKEKGIP-VYQSYGMTETASQIvtLSPE 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 502 NTVGAIGFVSRILpkiYPISI-IRADPdtgepirdrnglcQLCAPNEpgvfIGKI-VKGnPSREfLGYVD-EKASAKKIV 578
Cdd:PRK03640 301 DALTKLGSAGKPL---FPCELkIEKDG-------------VVVPPFE----EGEIvVKG-PNVT-KGYLNrEDATRETFQ 358
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 579 KDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVT 644
Cdd:PRK03640 359 DGWFKTGDIGYL-------DEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVP 417
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
176-708 |
4.14e-31 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 128.10 E-value: 4.14e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 176 YTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITP 254
Cdd:PRK07656 5 MTLPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGaVVVP 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 lINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSETEKNIPQAKNLNAllTTASYEKP 334
Cdd:PRK07656 85 -LNTRYTADEAAYILARGDAKALFVLGLFLGVDYSATTRLPALEHVVICETEEDDPHTEKMKTFTDFLAA--GDPAERAP 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 nktQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSI 414
Cdd:PRK07656 162 ---EVDPDDVADILFTSGTTGRPKGAMLTHRQLLSNAADWAEYLGLTEGDRYLAANPFFHVFGYKAGVNAPLMRGATILP 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 RKKFSASNYFADCAKYNATIGQYIGEMARYILAT-KPSEYDQKHrVRLVFGNG--LRPQIWPQFVQRFNIAKVGEFYGAT 491
Cdd:PRK07656 239 LPVFDPDEVFRLIETERITVLPGPPTMYNSLLQHpDRSAEDLSS-LRLAVTGAasMPVALLERFESELGVDIVLTGYGLS 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 492 EgnanimnhdntvgAIGFV--SRI--LPKIYPISIIRADPDTgePIRDRNGLCQLCAPNEPGVFigkIVKGnPSrEFLGY 567
Cdd:PRK07656 318 E-------------ASGVTtfNRLddDRKTVAGTIGTAIAGV--ENKIVNELGEEVPVGEVGEL---LVRG-PN-VMKGY 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 568 V-DEKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVE---AQVSNVAgykDTVVYGV 643
Cdd:PRK07656 378 YdDPEATAAAIDADGWLH------TGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEevlYEHPAVA---EAAVIGV 448
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 939619614 644 tiPHTE-GRAGMA-AIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQKE 708
Cdd:PRK07656 449 --PDERlGEVGKAyVVLKPGAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALREK 513
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
177-708 |
8.22e-31 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 127.94 E-value: 8.22e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSET-QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPL 255
Cdd:PRK06087 24 SLADYWQQTARAMPDKIAVVDNHgASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVP 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 INTNLRGPSLLHSIT-----VAHCSALIYGEDFLEAVTDVAKDLPanlTLFQFNNENNNSETEKNIPQAKNLnallttAS 330
Cdd:PRK06087 104 LLPSWREAELVWVLNkcqakMFFAPTLFKQTRPVDLILPLQNQLP---QLQQIVGVDKLAPATSSLSLSQII------AD 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 331 YEkPNKTQVN-HHDKLVYI-YTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLF 408
Cdd:PRK06087 175 YE-PLTTAITtHGDELAAVlFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLI 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 409 GSTVSIRKKFSASnyfadcakynATIGQYIGEMARYILATKPSEYD-----QKHRVRL------VFGNGLRPQIWPQFVQ 477
Cdd:PRK06087 254 GARSVLLDIFTPD----------ACLALLEQQRCTCMLGATPFIYDllnllEKQPADLsalrffLCGGTTIPKKVARECQ 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 478 RFNIaKVGEFYGATEGNANIM-NHDNTVGAIGFVSRIlpkiypisiiradPDTGEPIR----DRNGLcqlcapnEPGVFI 552
Cdd:PRK06087 324 QRGI-KLLSVYGSTESSPHAVvNLDDPLSRFMHTDGY-------------AAAGVEIKvvdeARKTL-------PPGCEG 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 553 GKIVKGnpSREFLGYVDEKASAKKIVKDvfkhgDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNV 632
Cdd:PRK06087 383 EEASRG--PNVFMGYLDEPELTARALDE-----EGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQH 455
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 633 AGYKDTVVYGVtiphTEGRAG-----MAAIYDPERELDL-DVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQ 706
Cdd:PRK06087 456 PKIHDACVVAM----PDERLGerscaYVVLKAPHHSLTLeEVVAFFSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLR 531
|
..
gi 939619614 707 KE 708
Cdd:PRK06087 532 KD 533
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
199-705 |
7.42e-29 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 120.23 E-value: 7.42e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 199 TQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALi 278
Cdd:cd05971 4 PEKVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASAL- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 279 ygedfleaVTDVAKDLpanltlfqfnnennnseteknipqaknlnALLttasyekpnktqvnhhdklvyIYTSGTTGLPK 358
Cdd:cd05971 83 --------VTDGSDDP-----------------------------ALI---------------------IYTSGTTGPPK 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 359 AAVISHSRYLFIAAGIHYTMGF--QEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRK--KFSASNYFADCAKYNATI 434
Cdd:cd05971 105 GALHAHRVLLGHLPGVQFPFNLfpRDGDLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRmtKFDPKAALDLMSRYGVTT 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 435 GQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQ----IWPQfvQRFNIAkVGEFYGATEGN------ANIMNHDNtv 504
Cdd:cd05971 185 AFLPPTALKMMRQQGEQLKHAQVKLRAIATGGESLGeellGWAR--EQFGVE-VNEFYGQTECNlvigncSALFPIKP-- 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 505 GAIGfvsrilpKIYPISIIRADPDTGEPIrdrnglcqlcAPNEPGvFIGkiVKGNPSREFLGYVD-EKASAKKIVKDVFK 583
Cdd:cd05971 260 GSMG-------KPIPGHRVAIVDDNGTPL----------PPGEVG-EIA--VELPDPVAFLGYWNnPSATEKKMAGDWLL 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 584 HGDMAfisgdllVADEKGYLYFKDRTGDTFRWKGENVSTSEVE------AQVSNVAgykdtvVYGVTIPhTEGRAGMAAI 657
Cdd:cd05971 320 TGDLG-------RKDSDGYFWYVGRDDDVITSSGYRIGPAEIEecllkhPAVLMAA------VVGIPDP-IRGEIVKAFV 385
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 939619614 658 YDPERELDLDVFAASL----AKVLPAYARPQIIRLLTKVDLTGTFKLRKVDL 705
Cdd:cd05971 386 VLNPGETPSDALAREIqelvKTRLAAHEYPREIEFVNELPRTATGKIRRREL 437
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
180-706 |
8.78e-29 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 121.45 E-value: 8.78e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVV-----SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITP 254
Cdd:cd05970 21 DVVDAMAKEYPDKLALVwcddaGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAI 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 LINTNLRGPSLLHSITVAHCSAL--IYGEDFLEAVTDVAKDLPANLTLFQfnnennnseTEKNIPQA-KNLNALLTTAS- 330
Cdd:cd05970 101 PATHQLTAKDIVYRIESADIKMIvaIAEDNIPEEIEKAAPECPSKPKLVW---------VGDPVPEGwIDFRKLIKNASp 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 331 -YEKPN-KTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTplpLYHTAGGIMCMGQ---S 405
Cdd:cd05970 172 dFERPTaNSYPCGEDILLVYFSSGTTGMPKMVEHDFTYPLGHIVTAKYWQNVREGGLHLT---VADTGWGKAVWGKiygQ 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 406 VLFGSTVSI--RKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYD-QKHRVRLVFGNGLRPQIWPQFVQRFNIa 482
Cdd:cd05970 249 WIAGAAVFVydYDKFDPKALLEKLSKYGVTTFCAPPTIYRFLIREDLSRYDlSSLRYCTTAGEALNPEVFNTFKEKTGI- 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 483 KVGEFYGATEgnanimnhdnTVGAIGFVSRILPKiyPISIIRADPDTGEPIRDRNGlcQLCAPNEPGVFIGKIVKGNPSR 562
Cdd:cd05970 328 KLMEGFGQTE----------TTLTIATFPWMEPK--PGSMGKPAPGYEIDLIDREG--RSCEAGEEGEIVIRTSKGKPVG 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 563 EFLGYVDEKASAKKIVKDVFKH-GDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVY 641
Cdd:cd05970 394 LFGGYYKDAEKTAEVWHDGYYHtGDAAWM-------DEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVT 466
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 939619614 642 GVTIPhTEGRAGMAAI-----YDPEREL--DLDVFAaslAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQ 706
Cdd:cd05970 467 GVPDP-IRGQVVKATIvlakgYEPSEELkkELQDHV---KKVTAPYKYPRIVEFVDELPKTISGKIRRVEIR 534
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
192-706 |
9.74e-29 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 119.87 E-value: 9.74e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 192 KVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITV 271
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 272 AHCSALIYGEDfleavtDVAkdlpanltlfqfnnennnseteknipqaknlnallttasyekpnktqvnhhdklVYIYTS 351
Cdd:cd05919 81 CEARLVVTSAD------DIA------------------------------------------------------YLLYSS 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 352 GTTGLPKAAVISHSRYLFIA-AGIHYTMGFQEEDIFYTPLPLYHTAGgimcMGQSVLF-----GSTVSIRKKFSASNYFA 425
Cdd:cd05919 101 GTTGPPKGVMHAHRDPLLFAdAMAREALGLTPGDRVFSSAKMFFGYG----LGNSLWFplavgASAVLNPGWPTAERVLA 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 426 DCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVF--GNGLRPQIWPQFVQRFNIaKVGEFYGATEgnaniMNH--- 500
Cdd:cd05919 177 TLARFRPTVLYGVPTFYANLLDSCAGSPDALRSLRLCVsaGEALPRGLGERWMEHFGG-PILDGIGATE-----VGHifl 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 501 DNTVGA--IGFVSRILPKiYPISiiradpdtgepIRDRNGlcQLCAPNEPGVFigkIVKGnPSrEFLGYVDEKASAKKIV 578
Cdd:cd05919 251 SNRPGAwrLGSTGRPVPG-YEIR-----------LVDEEG--HTIPPGEEGDL---LVRG-PS-AAVGYWNNPEKSRATF 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 579 KDVFkhgdmaFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPHTEGRAGMAAIY 658
Cdd:cd05919 312 NGGW------YRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAV--PESTGLSRLTAFV 383
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 939619614 659 DPERELDLDVFAA-----SLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQ 706
Cdd:cd05919 384 VLKSPAAPQESLArdihrHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
202-707 |
1.73e-28 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 118.60 E-value: 1.73e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 202 WTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSItvahcsaliygE 281
Cdd:cd05912 2 YTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQL-----------K 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 282 DfleavTDVAKDlpanltlfqfnnennnseteknipqaknlnallttasyekpnktqvnhhDKLVYIYTSGTTGLPKAAV 361
Cdd:cd05912 71 D-----SDVKLD-------------------------------------------------DIATIMYTSGTTGKPKGVQ 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 362 ISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTaGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEM 441
Cdd:cd05912 97 QTFGNHWWSAIGSALNLGLTEDDNWLCALPLFHI-SGLSILMRSVIYGMTVYLVDKFDAEQVLHLINSGKVTIISVVPTM 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 442 ARYILATKPSEYDQKHRVRLVfGNGLRPQIWPQFVQRFNIAkVGEFYGATEGNANI--MNHDNTVGAIGFVSRILPKIyP 519
Cdd:cd05912 176 LQRLLEILGEGYPNNLRCILL-GGGPAPKPLLEQCKEKGIP-VYQSYGMTETCSQIvtLSPEDALNKIGSAGKPLFPV-E 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 520 ISIIRADPDTGEPirdrnGLCQLCAPNepgvfigkIVKGnpsreFLGyvDEKASAKKIVKDVFKHGDMAFIsgdllvaDE 599
Cdd:cd05912 253 LKIEDDGQPPYEV-----GEILLKGPN--------VTKG-----YLN--RPDATEESFENGWFKTGDIGYL-------DE 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 600 KGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPhTEGRAGMAAIYDpERELDLDVFAASLAKVLPA 679
Cdd:cd05912 306 EGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDD-KWGQVPVAFVVS-ERPISEEELIAYCSEKLAK 383
|
490 500
....*....|....*....|....*...
gi 939619614 680 YARPQIIRLLTKVDLTGTFKLRKVDLQK 707
Cdd:cd05912 384 YKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
177-702 |
2.23e-27 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 117.57 E-value: 2.23e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQ--RWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITP 254
Cdd:PRK12583 19 TIGDAFDATVARFPDREALVVRHQalRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILV 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 LINTNLRGPSLLHSITVAHCSALIY-----GEDFLEAVTDVAKDL----PANLTLFQFNNENNNSETEKNIPQA----KN 321
Cdd:PRK12583 99 NINPAYRASELEYALGQSGVRWVICadafkTSDYHAMLQELLPGLaegqPGALACERLPELRGVVSLAPAPPPGflawHE 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 322 LNALLTTASYEKPNKTQ--VNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGI 399
Cdd:PRK12583 179 LQARGETVSREALAERQasLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVPVPLYHCFGMV 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 400 MCMGQSVLFGSTVSI-RKKFSASNYFADCAKYNATIGQ-----YIGEMaryilaTKP--SEYDQKH-RVRLVFGNGLRPQ 470
Cdd:PRK12583 259 LANLGCMTVGACLVYpNEAFDPLATLQAVEEERCTALYgvptmFIAEL------DHPqrGNFDLSSlRTGIMAGAPCPIE 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 471 IWPQFVQRFNIAKVGEFYGATEgnANIMNHDNTVG-----AIGFVSRILPKIyPISIIraDPDTGEPIRDRNGlcQLCap 545
Cdd:PRK12583 333 VMRRVMDEMHMAEVQIAYGMTE--TSPVSLQTTAAddlerRVETVGRTQPHL-EVKVV--DPDGATVPRGEIG--ELC-- 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 546 nepgvFIGKIVkgnpsreFLGYV-DEKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSE 624
Cdd:PRK12583 404 -----TRGYSV-------MKGYWnNPEATAESIDEDGWMH------TGDLATMDEQGYVRIVGRSKDMIIRGGENIYPRE 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 625 VEAQVSNVAGYKDTVVYGVtiPHTE-GRAGMAAI-YDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRK 702
Cdd:PRK12583 466 IEEFLFTHPAVADVQVFGV--PDEKyGEEIVAWVrLHPGHAASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQK 543
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
190-704 |
2.27e-27 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 115.70 E-value: 2.27e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLintNLRGPSllhs 268
Cdd:cd05930 1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGaAYVPL---DPSYPA---- 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 269 itvahcsaliygedflEAVTDVAKDLPANLtlfqfnnennnseteknipqaknlnaLLTtasyekpnktqvnHHDKLVY- 347
Cdd:cd05930 74 ----------------ERLAYILEDSGAKL--------------------------VLT-------------DPDDLAYv 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISHsrylfiAAGIHYTMGFQEEdifytplpLYHTAGGIMCMGQSVLF-------------GSTVSI 414
Cdd:cd05930 99 IYTSGSTGKPKGVMVEH------RGLVNLLLWMQEA--------YPLTPGDRVLQFTSFSFdvsvweifgallaGATLVV 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 RKKFSASN--YFAD-CAKYNATIGQYIGEMARYILATkpSEYDQKHRVRLVF--GNGLRPQIWPQFVQRFNIAKVGEFYG 489
Cdd:cd05930 165 LPEEVRKDpeALADlLAEEGITVLHLTPSLLRLLLQE--LELAALPSLRLVLvgGEALPPDLVRRWRELLPGARLVNLYG 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 490 ATEgnanimnhdNTVGAIGFvsRILPKIY---PISIIRADPDTGEPIRDRNGlcQLCAPNEPG-VFIGKIVKGnpsrefL 565
Cdd:cd05930 243 PTE---------ATVDATYY--RVPPDDEedgRVPIGRPIPNTRVYVLDENL--RPVPPGVPGeLYIGGAGLA------R 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 566 GYV-DEKASAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTgDT------FRwkgenVSTSEVEAQVSNVAGYKDT 638
Cdd:cd05930 304 GYLnRPELTAERFVPNPFGPGERMYRTGDLVRWLPDGNLEFLGRI-DDqvkirgYR-----IELGEIEAALLAHPGVREA 377
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 639 VVygVTIPHTEGRAGMAAIY--DPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLT--GtfklrKVD 704
Cdd:cd05930 378 AV--VAREDGDGEKRLVAYVvpDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTpnG-----KVD 440
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
191-707 |
2.55e-27 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 115.85 E-value: 2.55e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 191 DKVAVVSETQRWTFRQVNEHANKVANVLQAQGYK-KGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSI 269
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLLALGKDlRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 270 TVAHCSALIYGedfleavtdvakdlpanltlfqfnnennnseteknipqaknlnALLttasyekpnktqvnhhdklvyIY 349
Cdd:cd05941 81 TDSEPSLVLDP-------------------------------------------ALI---------------------LY 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 350 TSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAG---GIMCmgqSVLFGSTVSIRKKFSASNYFAD 426
Cdd:cd05941 97 TSGTTGRPKGVVLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVHGlvnALLC---PLFAGASVEFLPKFDPKEVAIS 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 427 CAKYNATI-----GQYIGEMARYILATKPSEYDQK---HRVRL-VFGNG-LRPQIWPQFVQRFNIAKVgEFYGATEGNAN 496
Cdd:cd05941 174 RLMPSITVfmgvpTIYTRLLQYYEAHFTDPQFARAaaaERLRLmVSGSAaLPVPTLEEWEAITGHTLL-ERYGMTEIGMA 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 497 IMNHDNTVGAIGFVSRILPKiypISIIRADPDTGEPIrDRNGLCQLCapnepgvfigkiVKGnPSReFLGYVDE-KASAK 575
Cdd:cd05941 253 LSNPLDGERRPGTVGMPLPG---VQARIVDEETGEPL-PRGEVGEIQ------------VRG-PSV-FKEYWNKpEATKE 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 576 KIVKDVFkhgdmaFISGDLLVADEKGYLYFKDRTG-DTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPHTEGRAGM 654
Cdd:cd05941 315 EFTDDGW------FKTGDLGVVDEDGYYWILGRSSvDIIKSGGYKVSALEIERVLLAHPGVSECAVIGV--PDPDWGERV 386
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 655 AAIYDPERE---LDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQK 707
Cdd:cd05941 387 VAVVVLRAGaaaLSLEELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
183-649 |
5.77e-27 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 115.56 E-value: 5.77e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 183 ERNVQAHPDKVAVV--SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNL 260
Cdd:PRK13391 4 GIHAQTTPDKPAVImaSTGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 261 RGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPaNLTLFQFNNENNNSETEKNIPQAknlnalltTASY-EKPNKTQV 339
Cdd:PRK13391 84 TPAEAAYIVDDSGARALITSAAKLDVARALLKQCP-GVRHRLVLDGDGELEGFVGYAEA--------VAGLpATPIADES 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 340 NHHDKLvyiYTSGTTGLPKA--AVISH---SRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCM-GQSvlFGSTVS 413
Cdd:PRK13391 155 LGTDML---YSSGTTGRPKGikRPLPEqppDTPLPLTAFLQRLWGFRSDMVYLSPAPLYHSAPQRAVMlVIR--LGGTVI 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 414 IRKKFSASNYFADCAKYNATIGQYIGEMARYILATkPSEYDQKHRVrlvfgNGLRPQI-----WPQFVQRFNIAKVG--- 485
Cdd:PRK13391 230 VMEHFDAEQYLALIEEYGVTHTQLVPTMFSRMLKL-PEEVRDKYDL-----SSLEVAIhaaapCPPQVKEQMIDWWGpii 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 486 -EFYGATEGN-------ANIMNHDNTVGAIGFvsrilpkiypisiiradpdtGEP-IRDRNGlcQLCAPNEPG-VFIgki 555
Cdd:PRK13391 304 hEYYAATEGLgftacdsEEWLAHPGTVGRAMF--------------------GDLhILDDDG--AELPPGEPGtIWF--- 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 556 vKGNPSREFLGYVDEKASAKKivkdvfKHGDMAFIsGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGY 635
Cdd:PRK13391 359 -EGGRPFEYLNDPAKTAEARH------PDGTWSTV-GDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKV 430
|
490
....*....|....
gi 939619614 636 KDTVVYGVtiPHTE 649
Cdd:PRK13391 431 ADAAVFGV--PNED 442
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
185-637 |
1.72e-26 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 114.25 E-value: 1.72e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 185 NVQAHPDKVAVV-SET-QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPL--INT- 258
Cdd:cd05904 14 FASAHPSRPALIdAATgRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGaVVTTAnpLSTp 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 259 -------NLRGPSLLhsITVAhcsaliygedfleavTDVAKDLPANLTLfqfnnennnseteKNIPQAKNLNA----LLT 327
Cdd:cd05904 94 aeiakqvKDSGAKLA--FTTA---------------ELAEKLASLALPV-------------VLLDSAEFDSLsfsdLLF 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 328 TASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRylFIAAGIHYTMGFQE----EDIFYTPLPLYHTAG-GIMCM 402
Cdd:cd05904 144 EADEAEPPVVVIKQDDVAALLYSSGTTGRSKGVMLTHRN--LIAMVAQFVAGEGSnsdsEDVFLCVLPMFHIYGlSSFAL 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 403 GQSVLfGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMaryILA----TKPSEYDQKHRVRLVFGNG-LRPQIWPQFVQ 477
Cdd:cd05904 222 GLLRL-GATVVVMPRFDLEELLAAIERYKVTHLPVVPPI---VLAlvksPIVDKYDLSSLRQIMSGAApLGKELIEAFRA 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 478 RFNIAKVGEFYGATEGNANIMNHDNTVGA---IGFVSRILP----KIypisiirADPDTGEPIrdrnglcqlcAPNEPG- 549
Cdd:cd05904 298 KFPNVDLGQGYGMTESTGVVAMCFAPEKDrakYGSVGRLVPnveaKI-------VDPETGESL----------PPNQTGe 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 550 VFI-G-KIVKgnpsreflGYV-DEKASAKKIVKDVFKH-GDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEV 625
Cdd:cd05904 361 LWIrGpSIMK--------GYLnNPEATAATIDKEGWLHtGDLCYI-------DEDGYLFIVDRLKELIKYKGFQVAPAEL 425
|
490 500
....*....|....*....|
gi 939619614 626 EA------QVSNVA--GYKD 637
Cdd:cd05904 426 EAlllshpEILDAAviPYPD 445
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
182-700 |
3.26e-26 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 113.21 E-value: 3.26e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 182 FERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLintNL 260
Cdd:cd17651 1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGaAYVPL---DP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 261 RGPS-LLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQfnnennnseteknipqaknLNALLTTASYEKPnktqv 339
Cdd:cd17651 78 AYPAeRLAFMLADAGPVLVLTHPALAGELAVELVAVTLLDQPG-------------------AAAGADAEPDPAL----- 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 340 nHHDKLVY-IYTSGTTGLPKAAVISHS----------RYLFIA--------AGIHYTMGFQEedIFyTPLplyhTAGGIM 400
Cdd:cd17651 134 -DADDLAYvIYTSGSTGRPKGVVMPHRslanlvawqaRASSLGpgartlqfAGLGFDVSVQE--IF-STL----CAGATL 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 401 CMgqsvlfgSTVSIRKKFSAsnyFAD-CAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQfVQRF 479
Cdd:cd17651 206 VL-------PPEEVRTDPPA---LAAwLDEQRISRVFLPTVALRALAEHGRPLGVRLAALRYLLTGGEQLVLTED-LREF 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 480 NIA----KVGEFYGATEgnanimNHDNTVGAIGFVSRILPKiyPISIIRADPDTGEPIRDRNGlcQLCAPNEPG-VFIGK 554
Cdd:cd17651 275 CAGlpglRLHNHYGPTE------THVVTALSLPGDPAAWPA--PPPIGRPIDNTRVYVLDAAL--RPVPPGVPGeLYIGG 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 555 IVKgnpSREFLGyvDEKASAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAG 634
Cdd:cd17651 345 AGL---ARGYLN--RPELTAERFVPDPFVPGARMYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPG 419
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 939619614 635 YKDTVVygVTIPHTEGRAGMAAIY--DPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd17651 420 VREAVV--LAREDRPGEKRLVAYVvgDPEAPVDAAELRAALATHLPEYMVPSAFVLLDALPLTPNGKL 485
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
190-647 |
5.65e-26 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 112.41 E-value: 5.65e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQR--WTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRgPSLLH 267
Cdd:cd05926 1 PDAPALVVPGSTpaLTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYK-KAEFE 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 268 SITVAHCSALIY--GEDFLEAVTDVAKDLPANLTLfqfnneNNNSETEKNIPQAKNLNALLTTASYEKPNKTQVNHHDKL 345
Cdd:cd05926 80 FYLADLGSKLVLtpKGELGPASRAASKLGLAILEL------ALDVGVLIRAPSAESLSNLLADKKNAKSEGVPLPDDLAL 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 346 VyIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHtAGGIMCMGQSVLF-GSTVSIRKKFSASNYF 424
Cdd:cd05926 154 I-LHTSGTTGRPKGVPLTHRNLAASATNITNTYKLTPDDRTLVVMPLFH-VHGLVASLLSTLAaGGSVVLPPRFSASTFW 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 425 ADCAKYNATIGQYIGEMARYILAT-KPSEYDQKHRVRLV--FGNGLRPQIWPQFVQRFNiAKVGEFYGATEG----NANI 497
Cdd:cd05926 232 PDVRDYNATWYTAVPTIHQILLNRpEPNPESPPPKLRFIrsCSASLPPAVLEALEATFG-APVLEAYGMTEAahqmTSNP 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 498 MNHD----NTVGaIGFVSRIlpKIYpisiiradPDTGEPIrdrnglcqlcapnEPGVfIGKI-VKG-NPSReflGYV-DE 570
Cdd:cd05926 311 LPPGprkpGSVG-KPVGVEV--RIL--------DEDGEIL-------------PPGV-VGEIcLRGpNVTR---GYLnNP 362
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 939619614 571 KASAKKIVKDVFkhgdmaFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPH 647
Cdd:cd05926 363 EANAEAAFKDGW------FRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGV--PD 431
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
193-702 |
1.05e-25 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 111.53 E-value: 1.05e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 193 VAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGV-ITPlINTNLRGPSLLHSITV 271
Cdd:PRK08276 3 VIMAPSGEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLyYTP-INWHLTAAEIAYIVDD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 272 AHCSALIYGEDFLEAVTDVAKDLPANLTLFqfnnennnSETEKNIPQAKNLNALLTTASyEKPNKTQVNHHDKLvyiYTS 351
Cdd:PRK08276 82 SGAKVLIVSAALADTAAELAAELPAGVPLL--------LVVAGPVPGFRSYEEALAAQP-DTPIADETAGADML---YSS 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 352 GTTGLPKA------AVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMgQSVLFGSTVSIRKKFSASNYFA 425
Cdd:PRK08276 150 GTTGRPKGikrplpGLDPDEAPGMMLALLGFGMYGGPDSVYLSPAPLYHTAPLRFGM-SALALGGTVVVMEKFDAEEALA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 426 DCAKYNATIGQYIGEMARYILATkPSEYDQKHRVRlvfgnGLRPQI-----WPQFVQRFNIAKVG----EFYGATEGN-- 494
Cdd:PRK08276 229 LIERYRVTHSQLVPTMFVRMLKL-PEEVRARYDVS-----SLRVAIhaaapCPVEVKRAMIDWWGpiihEYYASSEGGgv 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 495 -----ANIMNHDNTVG-AIGFVSRILpkiypisiiraDPDtGEPirdrnglcqlCAPNEPG-VFIGkivKGNPSREFLGy 567
Cdd:PRK08276 303 tvitsEDWLAHPGSVGkAVLGEVRIL-----------DED-GNE----------LPPGEIGtVYFE---MDGYPFEYHN- 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 568 vDEKASAKKivkdvfKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPH 647
Cdd:PRK08276 357 -DPEKTAAA------RNPHGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGV--PD 427
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 648 TE-GRAGMAAI-----YDPERELDLDVfAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRK 702
Cdd:PRK08276 428 EEmGERVKAVVqpadgADAGDALAAEL-IAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYK 487
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
177-686 |
1.30e-25 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 112.02 E-value: 1.30e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVI---- 252
Cdd:PRK05605 33 TLVDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVvveh 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 253 TPLINTN-LRGPSLLHSITVA--------HCSALIyGEDFLEAVtdVAKDLPANLTLFQfnnennNSETEKNIPQAKNLN 323
Cdd:PRK05605 113 NPLYTAHeLEHPFEDHGARVAivwdkvapTVERLR-RTTPLETI--VSVNMIAAMPLLQ------RLALRLPIPALRKAR 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 324 ALLT-----TASYEK-------PNKTQVNH-----HDKLVYIYTSGTTGLPKAAVISHsRYLF--IAAGIHYTMGFQEED 384
Cdd:PRK05605 184 AALTgpapgTVPWETlvdaaigGDGSDVSHprptpDDVALILYTSGTTGKPKGAQLTH-RNLFanAAQGKAWVPGLGDGP 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 385 -IFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATigqyigemaryILATKPSEYD------QKH 457
Cdd:PRK05605 263 eRVLAALPMFHAYGLTLCLTLAVSIGGELVLLPAPDIDLILDAMKKHPPT-----------WLPGVPPLYEkiaeaaEER 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 458 RVRLvfgNGLRPQIW------PQFVQRFNIAKVG---EFYGATEGN----ANIMNHDNTVGAIGfvsriLPkiYPISIIR 524
Cdd:PRK05605 332 GVDL---SGVRNAFSgamalpVSTVELWEKLTGGllvEGYGLTETSpiivGNPMSDDRRPGYVG-----VP--FPDTEVR 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 525 -ADPDTgePIRDRnglcqlcAPNEPGVFigkIVKGnPSReFLGYV-DEKASAKKIVKDVFKHGDMAfisgdllVADEKGY 602
Cdd:PRK05605 402 iVDPED--PDETM-------PDGEEGEL---LVRG-PQV-FKGYWnRPEETAKSFLDGWFRTGDVV-------VMEEDGF 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 603 LYFKDR------TGdtfrwkGENVSTSEVEAQVSNVAGYKDTVVYGvtIPHTEGRAGMAA--IYDPERELDLDVFAASLA 674
Cdd:PRK05605 461 IRIVDRikeliiTG------GFNVYPAEVEEVLREHPGVEDAAVVG--LPREDGSEEVVAavVLEPGAALDPEGLRAYCR 532
|
570
....*....|..
gi 939619614 675 KVLPAYARPQII 686
Cdd:PRK05605 533 EHLTRYKVPRRF 544
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
180-700 |
3.65e-25 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 109.60 E-value: 3.65e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLint 258
Cdd:cd12117 1 ELFEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGaAYVPL--- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 259 NLRGPS--LLHSITVAHCSALIYgedfLEAVTDVAKDLPANLTLFQFNNENnnseteknipQAKNLNAllttasyekpnk 336
Cdd:cd12117 78 DPELPAerLAFMLADAGAKVLLT----DRSLAGRAGGLEVAVVIDEALDAG----------PAGNPAV------------ 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 337 tqVNHHDKLVYI-YTSGTTGLPKAAVISHSRYLFIAAGIHYtMGFQEEDIFYTPLPLYHTAGGIMCMGqSVLFGSTVSIr 415
Cdd:cd12117 132 --PVSPDDLAYVmYTSGSTGRPKGVAVTHRGVVRLVKNTNY-VTLGPDDRVLQTSPLAFDASTFEIWG-ALLNGARLVL- 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 416 kkfSASNYFADCAKynatIGQYIGEMARYILATKPSEYDQKHRVRL-VFGnGLRpQIW-------PQFVQRFNIA----K 483
Cdd:cd12117 207 ---APKGTLLDPDA----LGALIAEEGVTVLWLTAALFNQLADEDPeCFA-GLR-ELLtggevvsPPHVRRVLAAcpglR 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 484 VGEFYGATEgnanimnhdNTVGAIGF-VSRILPKIYPISIIRADPDTGEPIRDRNGlcQLCAPNEPG-VFIGKI-VKgnp 560
Cdd:cd12117 278 LVNGYGPTE---------NTTFTTSHvVTELDEVAGSIPIGRPIANTRVYVLDEDG--RPVPPGVPGeLYVGGDgLA--- 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 561 srefLGYV-DEKASAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTV 639
Cdd:cd12117 344 ----LGYLnRPALTAERFVADPFGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAV 419
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 640 VygVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd12117 420 V--VVREDAGGDKRLVAYVVAEGALDAAELRAFLRERLPAYMVPAAFVVLDELPLTANGKV 478
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
211-699 |
4.92e-25 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 109.07 E-value: 4.92e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 211 ANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-----VITPLinTNLRGPSLLHSItVAHCSALIYgedFLE 285
Cdd:cd05922 3 VSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGgrlglVFVPL--NPTLKESVLRYL-VADAGGRIV---LAD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 286 --AVTDVAKDLPAnltlfqfnnennnSETEKNIPQAKNLNALLTTASyekpnKTQVNHHDKLVYIYTSGTTGLPKAAVIS 363
Cdd:cd05922 77 agAADRLRDALPA-------------SPDPGTVLDADGIRAARASAP-----AHEVSHEDLALLLYTSGSTGSPKLVRLS 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 364 HSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHtaggimCMGQSVLF-----GSTVSIRKKFSASNYF-ADCAKYNATigqy 437
Cdd:cd05922 139 HQNLLANARSIAEYLGITADDRALTVLPLSY------DYGLSVLNthllrGATLVLTNDGVLDDAFwEDLREHGAT---- 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 438 igemaryILATKPSEYDQKHRV----------RLVFGNGLR-PQiwpQFVQRFNIAKVGE----FYGATEGNANImnhdN 502
Cdd:cd05922 209 -------GLAGVPSTYAMLTRLgfdpaklpslRYLTQAGGRlPQ---ETIARLRELLPGAqvyvMYGQTEATRRM----T 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 503 TVGAigfvSRILPKiyPISIIRADPDTGEPIRDRNGlcQLCAPNEPG--VFIGKIVkgnpsreFLGYVDEKASAKKIVKd 580
Cdd:cd05922 275 YLPP----ERILEK--PGSIGLAIPGGEFEILDDDG--TPTPPGEPGeiVHRGPNV-------MKGYWNDPPYRRKEGR- 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 581 vfkhGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYDp 660
Cdd:cd05922 339 ----GGGVLHTGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDPLGEKLALFVTAPD- 413
|
490 500 510
....*....|....*....|....*....|....*....
gi 939619614 661 erELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFK 699
Cdd:cd05922 414 --KIDPKDVLRSLAERLPPYKVPATVRVVDELPLTASGK 450
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
177-705 |
7.13e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 109.45 E-value: 7.13e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLI 256
Cdd:PRK06164 11 TLASLLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAV 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHSITVAHCSALIY-----GEDFLEAVTDVAKDLPANLtlfqfNNENNNSETEKNIP--------QAKNLN 323
Cdd:PRK06164 91 NTRYRSHEVAHILGRGRARWLVVwpgfkGIDFAAILAAVPPDALPPL-----RAIAVVDDAADATPapapgarvQLFALP 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 324 ALLTTASYEKPNKTQvnhhDKLVYIY-TSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCM 402
Cdd:PRK06164 166 DPAPPAAAGERAADP----DAGALLFtTSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCGVFGFSTLL 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 403 GqSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGL-RPQIWPQFVQRFNI 481
Cdd:PRK06164 242 G-ALAGGAPLVCEPVFDAARTARALRRHRVTHTFGNDEMLRRILDTAGERADFPSARLFGFASFApALGELAALARARGV 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 482 AKVGeFYGATEGNANIMNHDNTvgaIGFVSRILP---KIYPISIIRA-DPDTGEpirdrnglcqLCAPNEPgvfiGKIVK 557
Cdd:PRK06164 321 PLTG-LYGSSEVQALVALQPAT---DPVSVRIEGggrPASPEARVRArDPQDGA----------LLPDGES----GEIEI 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 558 GNPSReFLGYVDE-KASAKKIVKD-VFKHGDMAFISGDllvadekGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGY 635
Cdd:PRK06164 383 RAPSL-MRGYLDNpDATARALTDDgYFRTGDLGYTRGD-------GQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGV 454
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 939619614 636 KDTVVYGVTIphtEGRAGMAA--IYDPERELDLDVFAASLAKVLPAY---ARPQIIRLLTKVDLTGTFKLRKVDL 705
Cdd:PRK06164 455 AAAQVVGATR---DGKTVPVAfvIPTDGASPDEAGLMAACREALAGFkvpARVQVVEAFPVTESANGAKIQKHRL 526
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
190-667 |
7.63e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 108.82 E-value: 7.63e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSI 269
Cdd:PRK06145 16 PDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEVAYIL 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 270 TVAHCSALIYGEDFLEAVTDVAKDLPANltlfqfnnennnsetekniPQAKNLNALLTTASYEKPNKTQVNHHDKLVYIY 349
Cdd:PRK06145 96 GDAGAKLLLVDEEFDAIVALETPKIVID-------------------AAAQADSRRLAQGGLEIPPQAAVAPTDLVRLMY 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 350 TSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTaGGIMCMGQSVLF-GSTVSIRKKFSASNYFADCA 428
Cdd:PRK06145 157 TSGTTDRPKGVMHSYGNLHWKSIDHVIALGLTASERLLVVGPLYHV-GAFDLPGIAVLWvGGTLRIHREFDPEAVLAAIE 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 429 KYNATIGQYIGEMARYILAT-KPSEYDQKhRVRLVFGNGLRP--QIWPQFVQRFNIAKVGEFYGATE--GNANIMNHDNT 503
Cdd:PRK06145 236 RHRLTCAWMAPVMLSRVLTVpDRDRFDLD-SLAWCIGGGEKTpeSRIRDFTRVFTRARYIDAYGLTEtcSGDTLMEAGRE 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 504 VGAIGFVSRILPKIyPISIirADPDTGEPIRDRNGLCQLCAPnepgvfigKIVKGNpsreflgYVDEKASAKKIVKDVFK 583
Cdd:PRK06145 315 IEKIGSTGRALAHV-EIRI--ADGAGRWLPPNMKGEICMRGP--------KVTKGY-------WKDPEKTAEAFYGDWFR 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 584 HGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYDPERE 663
Cdd:PRK06145 377 SGDVGYL-------DEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGAT 449
|
....
gi 939619614 664 LDLD 667
Cdd:PRK06145 450 LTLE 453
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
163-706 |
8.13e-25 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 109.46 E-value: 8.13e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 163 KLLRYTKRHERLNYTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVAT 242
Cdd:PRK13382 30 RYLRIVAAMRREGMGPTSGFAIAAQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEA 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 243 WLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQFNNEnnnsetekniPQAKNL 322
Cdd:PRK13382 110 LLAANRIGADILLLNTSFAGPALAEVVTREGVDTVIYDEEFSATVDRALADCPQATRIVAWTDE----------DHDLTV 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 323 NALLTTASYEKPNKTqvNHHDKLVyIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGgimcM 402
Cdd:PRK13382 180 EVLIAAHAGQRPEPT--GRKGRVI-LLTSGTTGTPKGARRSGPGGIGTLKAILDRTPWRAEEPTVIVAPMFHAWG----F 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 403 GQSVL---FGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVF----GNGLRPQIWPQF 475
Cdd:PRK13382 253 SQLVLaasLACTIVTRRRFDPEATLDLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFaaasGSRMRPDVVIAF 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 476 VQRFNIAkVGEFYGATEGN-------ANIMNHDNTVG--AIGFVSRILpkiypisiiraDPDTGEpirdrnglcqlCAPN 546
Cdd:PRK13382 333 MDQFGDV-IYNNYNATEAGmiatatpADLRAAPDTAGrpAEGTEIRIL-----------DQDFRE-----------VPTG 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 547 EpgvfIGKIVKGNpSREFLGYVDEKAsakkivKDvFKHGDMAfiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVE 626
Cdd:PRK13382 390 E----VGTIFVRN-DTQFDGYTSGST------KD-FHDGFMA--SGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVE 455
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 627 AQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQ 706
Cdd:PRK13382 456 KTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGASATPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQ 535
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
180-707 |
1.54e-24 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 107.01 E-value: 1.54e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTN 259
Cdd:cd17653 1 DAFERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAK 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 260 LrgPSllhsitvahcsaliygedflEAVTDVAKDLPANLtlfqfnnennnseteknipqaknlnaLLTTASyekpnktqv 339
Cdd:cd17653 81 L--PS--------------------ARIQAILRTSGATL--------------------------LLTTDS--------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 340 nHHDKLVYIYTSGTTGLPKAAVISHSRYLfiaagiHYtmgfqeedIFYTPLPLYHTAGGIMCMGQSVLFgsTVSIRKKFS 419
Cdd:cd17653 104 -PDDLAYIIFTSGSTGIPKGVMVPHRGVL------NY--------VSQPPARLDVGPGSRVAQVLSIAF--DACIGEIFS 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 420 A------------SNYFADCAKY-NATIgqyigeMARYILAT-KPSEYDqkhRVRLVF--GNGLRPQI---WPQFVQRFN 480
Cdd:cd17653 167 TlcnggtlvladpSDPFAHVARTvDALM------STPSILSTlSPQDFP---NLKTIFlgGEAVPPSLldrWSPGRRLYN 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 481 iakvgeFYGATEgnanimnhdNTVGAigFVSRILPkIYPISIiradpdtGEPIrdRNGLCQLCAPNE---PGVFIGKIVK 557
Cdd:cd17653 238 ------AYGPTE---------CTISS--TMTELLP-GQPVTI-------GKPI--PNSTCYILDADLqpvPEGVVGEICI 290
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 558 GNPSREfLGYV-DEKASAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVstsEVEAQVSNVAGYK 636
Cdd:cd17653 291 SGVQVA-RGYLgNPALTASKFVPDPFWPGSRMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRI---NLEEIEEVVLQSQ 366
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 637 DTVVYGVTIPHtEGRagMAAIYDPErELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTfklRKVDLQK 707
Cdd:cd17653 367 PEVTQAAAIVV-NGR--LVAFVTPE-TVDVDGLRSELAKHLPSYAVPDRIIALDSFPLTAN---GKVDRKA 430
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
172-626 |
6.62e-24 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 106.43 E-value: 6.62e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 172 ERLNYTVADVFERNVQAHPDKVAVVSETQ--RWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKI 249
Cdd:PRK08315 12 PLLEQTIGQLLDRTAARYPDREALVYRDQglRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKI 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 250 GVITPLINTNLRGPSLLHSITVAHCSALIYGE-----DFLEAVTDVAKDL----PANLTLFQFnnennnsetekniPQAK 320
Cdd:PRK08315 92 GAILVTINPAYRLSELEYALNQSGCKALIAADgfkdsDYVAMLYELAPELatcePGQLQSARL-------------PELR 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 321 N-----------------LNALLTTASYEK--PNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYL----FIAAgihyT 377
Cdd:PRK08315 159 RviflgdekhpgmlnfdeLLALGRAVDDAElaARQATLDPDDPINIQYTSGTTGFPKGATLTHRNILnngyFIGE----A 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 378 MGFQEEDIFYTPLPLYHTAGgiMCMGQ--SVLFGST-VSIRKKFsasnyfaDCAKYNATIGQ------------YIGEM- 441
Cdd:PRK08315 235 MKLTEEDRLCIPVPLYHCFG--MVLGNlaCVTHGATmVYPGEGF-------DPLATLAAVEEerctalygvptmFIAELd 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 442 ----ARYILATkpseydqkhrvrlvfgngLRPQIW-----P-----QFVQRFNIAKVGEFYGATEG---------NANIM 498
Cdd:PRK08315 306 hpdfARFDLSS------------------LRTGIMagspcPievmkRVIDKMHMSEVTIAYGMTETspvstqtrtDDPLE 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 499 NHDNTVGaigfvsRILPKIyPISIIraDPDTGEPirdrnglcqlCAPNEPGVFI--GKIVkgnpsreFLGYV-DEKASAK 575
Cdd:PRK08315 368 KRVTTVG------RALPHL-EVKIV--DPETGET----------VPRGEQGELCtrGYSV-------MKGYWnDPEKTAE 421
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 939619614 576 KIVKDVFKHgdmafiSGDLLVADEKGYLYFkdrTGdtfRWK------GENVSTSEVE 626
Cdd:PRK08315 422 AIDADGWMH------TGDLAVMDEEGYVNI---VG---RIKdmiirgGENIYPREIE 466
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
177-642 |
7.73e-24 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 106.72 E-value: 7.73e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVS----ETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVI 252
Cdd:COG1022 12 TLPDLLRRRAARFPDRVALREkedgIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 253 T-PLINTNLrgPSLLHSItVAHCSA---LIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSEtekniPQAKNLNALLTT 328
Cdd:COG1022 92 TvPIYPTSS--AEEVAYI-LNDSGAkvlFVEDQEQLDKLLEVRDELPSLRHIVVLDPRGLRDD-----PRLLSLDELLAL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 329 -ASYEKPN-----KTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCM 402
Cdd:COG1022 164 gREVADPAelearRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVFERTVSY 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 403 GQsVLFGSTV---------------------------------SIRKKFSAS--------NYFADCAKynatigqyigEM 441
Cdd:COG1022 244 YA-LAAGATVafaespdtlaedlrevkptfmlavprvwekvyaGIQAKAEEAgglkrklfRWALAVGR----------RY 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 442 ARYILATK--PSEYDQKHRV--RLVFGNgLR----PQIW---------PQFVQRFNIA---KVGEFYGATEgnanimnhd 501
Cdd:COG1022 313 ARARLAGKspSLLLRLKHALadKLVFSK-LRealgGRLRfavsggaalGPELARFFRAlgiPVLEGYGLTE--------- 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 502 nTVGAIgFVSRilPKiypisiiRADPDT-GEPIrdrnglcqlcapnePGVFIgKI-------VKGnPSReFLGY-VDEKA 572
Cdd:COG1022 383 -TSPVI-TVNR--PG-------DNRIGTvGPPL--------------PGVEV-KIaedgeilVRG-PNV-MKGYyKNPEA 434
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 939619614 573 SAKKIVKD-VFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFrwK---GENVSTSEVEAQVSNVAGYKDTVVYG 642
Cdd:COG1022 435 TAEAFDADgWLHTGDIGEL-------DEDGFLRITGRKKDLI--VtsgGKNVAPQPIENALKASPLIEQAVVVG 499
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
176-657 |
8.65e-24 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 105.71 E-value: 8.65e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 176 YTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLGLSKIGVITP 254
Cdd:PRK06839 2 QGIAYWIEKRAYLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 LINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKdlpanltlfqfnnennnsetEKNIPQAKNLNALLTTASYEKP 334
Cdd:PRK06839 82 PLNIRLTENELIFQLKDSGTTVLFVEKTFQNMALSMQK--------------------VSYVQRVISITSLKEIEDRKID 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 NKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTaGGIMCMGQSVLF-GSTVS 413
Cdd:PRK06839 142 NFVEKNESASFIICYTSGTTGKPKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPLFHI-GGIGLFAFPTLFaGGVII 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 414 IRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLvFGNGLRP---QIWPQFVQRFniAKVGEFYGA 490
Cdd:PRK06839 221 VPRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETTNLQSVRW-FYNGGAPcpeELMREFIDRG--FLFGQGFGM 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 491 TEGNANIM-----NHDNTVGAIGfvsrilpKIYPISIIRA-DPDTGEPirdrnglcqlcapnEPGVfIGKI-VKG-NPSR 562
Cdd:PRK06839 298 TETSPTVFmlseeDARRKVGSIG-------KPVLFCDYELiDENKNKV--------------EVGE-VGELlIRGpNVMK 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 563 EFlgYVDEKASAKKIvkdvfkhGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYG 642
Cdd:PRK06839 356 EY--WNRPDATEETI-------QDGWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVG 426
|
490
....*....|....*.
gi 939619614 643 VtiPHTE-GRAGMAAI 657
Cdd:PRK06839 427 R--QHVKwGEIPIAFI 440
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
176-454 |
1.02e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 105.74 E-value: 1.02e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 176 YTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVItpL 255
Cdd:PRK07798 3 WNIADLFEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAV--P 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 INTNLR-GPSLLHSI-TVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSETekniPQAKNLNALLTTASYEK 333
Cdd:PRK07798 81 VNVNYRyVEDELRYLlDDSDAVALVYEREFAPRVAEVLPRLPKLRTLVVVEDGSGNDLL----PGAVDYEDALAAGSPER 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 334 PNKTQVNhhDKLVYIYTSGTTGLPKAAVISHSRYLFIAAG--IHYTMGF--QEEDI------------FYTPlPLYHTAG 397
Cdd:PRK07798 157 DFGERSP--DDLYLLYTGGTTGMPKGVMWRQEDIFRVLLGgrDFATGEPieDEEELakraaagpgmrrFPAP-PLMHGAG 233
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 939619614 398 GIMCMGqSVLFGSTVSI--RKKFSASNYFADCAKYNATIGQYIGE-MARYILAT--KPSEYD 454
Cdd:PRK07798 234 QWAAFA-ALFSGQTVVLlpDVRFDADEVWRTIEREKVNVITIVGDaMARPLLDAleARGPYD 294
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
183-643 |
1.38e-23 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 105.07 E-value: 1.38e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 183 ERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRG 262
Cdd:cd12118 11 ERAAAVYPDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 263 PSLLHSITVAHCSALIYGEDFleavtdvakdlpanltlfqfnnennnseteknipqakNLNALLTTASYEKPNKTQVNHH 342
Cdd:cd12118 91 EEIAFILRHSEAKVLFVDREF-------------------------------------EYEDLLAEGDPDFEWIPPADEW 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 343 DKLVYIYTSGTTGLPKAAVISH-SRYL-FIAAGIHYTMgfQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKkFSA 420
Cdd:cd12118 134 DPIALNYTSGTTGRPKGVVYHHrGAYLnALANILEWEM--KQHPVYLWTLPMFHCNGWCFPWTVAAVGGTNVCLRK-VDA 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 421 SNYFADCAKYNATigQYIGEMARYILATKPSEYDQK---HRVRLVFGNGLRPQIWPQFVQRFNIaKVGEFYGATE--GNA 495
Cdd:cd12118 211 KAIYDLIEKHKVT--HFCGAPTVLNMLANAPPSDARplpHRVHVMTAGAPPPAAVLAKMEELGF-DVTHVYGLTEtyGPA 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 496 NI-----------------MNHDNTVGAIGFvsrilpkiYPISIIraDPDTGEPI-RDrnglcqlcapnepGVFIGKIV- 556
Cdd:cd12118 288 TVcawkpewdelpteerarLKARQGVRYVGL--------EEVDVL--DPETMKPVpRD-------------GKTIGEIVf 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 557 KGNPSreFLGYV-DEKASAKkivkdVFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGY 635
Cdd:cd12118 345 RGNIV--MKGYLkNPEATAE-----AFRGG--WFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAV 415
|
....*...
gi 939619614 636 KDTVVYGV 643
Cdd:cd12118 416 LEAAVVAR 423
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
188-706 |
1.81e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 104.50 E-value: 1.81e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 188 AHPDKVAVV--SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSL 265
Cdd:PRK09088 7 LQPQRLAAVdlALGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASEL 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 266 lHSITVAHCSALIYGEDFLEAVTDVAKDLPAnltlfqfnnennnsetekNIPQAKNLNALLT-TASYEKPNktqvnhhdk 344
Cdd:PRK09088 87 -DALLQDAEPRLLLGDDAVAAGRTDVEDLAA------------------FIASADALEPADTpSIPPERVS--------- 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 345 lVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYF 424
Cdd:PRK09088 139 -LILFTSGTSGQPKGVMLSERNLQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGFEPKRTL 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 425 ADCAKYNATIGQYIG--EMARYILAtKPSeYDQ---KHRVRLVFGNGLRP----QIWPQ----FVQRFNIAKVGEFYGAT 491
Cdd:PRK09088 218 GRLGDPALGITHYFCvpQMAQAFRA-QPG-FDAaalRHLTALFTGGAPHAaediLGWLDdgipMVDGFGMSEAGTVFGMS 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 492 EGNANImnhDNTVGAIGFvsrilpkiypisiirADPDTGEPIRDRNGlcQLCAPNEPGVFIgkiVKGnPSReFLGY-VDE 570
Cdd:PRK09088 296 VDCDVI---RAKAGAAGI---------------PTPTVQTRVVDDQG--NDCPAGVPGELL---LRG-PNL-SPGYwRRP 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 571 KASAkkivkDVFKhGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTeG 650
Cdd:PRK09088 351 QATA-----RAFT-GDGWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQW-G 423
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 939619614 651 RAGMAAI-YDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQ 706
Cdd:PRK09088 424 EVGYLAIvPADGAPLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARLR 480
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
194-691 |
3.71e-23 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 104.01 E-value: 3.71e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 194 AVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLEN-----RAEYVATWLGlskiGVITPlINTNLRGPSLLHS 268
Cdd:PRK12406 4 TIISGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNdfaffEAAYAAMRLG----AYAVP-VNWHFKPEEIAYI 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 269 ITVAHCSALIYGEDFLEAVtdvAKDLPANLTLFQFNNENNNSETEKNIPQAKNLNA--------LLTTASYEKPNKTQVN 340
Cdd:PRK12406 79 LEDSGARVLIAHADLLHGL---ASALPAGVTVLSVPTPPEIAAAYRISPALLTPPAgaidwegwLAQQEPYDGPPVPQPQ 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 341 HhdklvYIYTSGTTGLPK-----AAVISHSR-YLFIAAGIHytmGFQEEDIFYTPLPLYHTAGGIMCMgQSVLFGSTVSI 414
Cdd:PRK12406 156 S-----MIYTSGTTGHPKgvrraAPTPEQAAaAEQMRALIY---GLKPGIRALLTGPLYHSAPNAYGL-RAGRLGGVLVL 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 RKKFSASNYFADCAKYNATIGQYIGEMARYILATkPSEYDQKHRV---RLVFgNGLRPqiWPQFVQRFNIAKVG----EF 487
Cdd:PRK12406 227 QPRFDPEELLQLIERHRITHMHMVPTMFIRLLKL-PEEVRAKYDVsslRHVI-HAAAP--CPADVKRAMIEWWGpviyEY 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 488 YGATEGNA-------NIMNHDNTVGaigfvsrilpKIYPISIIRADPDTGEPIrdrnglcqlcAPNEPGVFIGKIvKGNP 560
Cdd:PRK12406 303 YGSTESGAvtfatseDALSHPGTVG----------KAAPGAELRFVDEDGRPL----------PQGEIGEIYSRI-AGNP 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 561 SREFLGYVDEKASAKKIvkdvfkhgdmAFI-SGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTV 639
Cdd:PRK12406 362 DFTYHNKPEKRAEIDRG----------GFItSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCA 431
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 939619614 640 VYGvtIPHTE-GRAGMAAIY-DPERELDLDVFAASLAKVLPAYARPQIIRLLTK 691
Cdd:PRK12406 432 VFG--IPDAEfGEALMAVVEpQPGATLDEADIRAQLKARLAGYKVPKHIEIMAE 483
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
192-705 |
1.14e-22 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 101.79 E-value: 1.14e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 192 KVAVVSETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLH--- 267
Cdd:cd05958 1 RTCLRSPEREWTYRDLLALANRIANVLVGElGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYild 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 268 --SITVAHCSaliygeDFLEAVTDVAkdlpanltlfqfnnennnseteknipqaknlnallttasyekpnktqvnhhdkl 345
Cdd:cd05958 81 kaRITVALCA------HALTASDDIC------------------------------------------------------ 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 346 VYIYTSGTTGLPKAAVISHSRYLFIAA--GIHyTMGFQEEDIFYTPLPLYHTAGgimcMGQSVLF----GSTVSIRKKFS 419
Cdd:cd05958 101 ILAFTSGTTGAPKATMHFHRDPLASADryAVN-VLRLREDDRFVGSPPLAFTFG----LGGVLLFpfgvGASGVLLEEAT 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 420 ASNYFADCAKYNATIGQYIGEMARYILATKP-SEYDQKHRVRLV-FGNGLRPQIWPQFVQRFNIaKVGEFYGATEgnani 497
Cdd:cd05958 176 PDLLLSAIARYKPTVLFTAPTAYRAMLAHPDaAGPDLSSLRKCVsAGEALPAALHRAWKEATGI-PIIDGIGSTE----- 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 498 MNH---DNTVGAI--GFVSRILPKiYPISIIradPDTGEPIrdrnglcqlcapnePGVFIGKIVKGNPSreFLGYVDEKA 572
Cdd:cd05958 250 MFHifiSARPGDArpGATGKPVPG-YEAKVV---DDEGNPV--------------PDGTIGRLAVRGPT--GCRYLADKR 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 573 SAKKIvkdvfkhGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIP------ 646
Cdd:cd05958 310 QRTYV-------QGGWNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDEsrgvvv 382
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 647 --HTEGRAGMAAIYDPERELDlDVFAASLAKvlpaYARPQIIRLLTKVDLTGTFKLRKVDL 705
Cdd:cd05958 383 kaFVVLRPGVIPGPVLARELQ-DHAKAHIAP----YKYPRAIEFVTELPRTATGKLQRFAL 438
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
140-702 |
1.22e-22 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 103.04 E-value: 1.22e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 140 GWRWFYIAGATASRDLTALWAyikllRYTkRHERLNyTVADVFERNVQAHPDKVAVV------SETQRWTFRQVNEHANK 213
Cdd:cd17634 24 DWITPYQKVKNTSFAPGAPSI-----KWF-EDATLN-LAANALDRHLRENGDRTAIIyegddtSQSRTISYRELHREVCR 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 214 VANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYGEDFLEA------V 287
Cdd:cd17634 97 FAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLITADGGVRAgrsvplK 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 288 TDVAKDLPANLTLFQFNNENNNSETEKNIPQAKNL--NALLTTASYEKpNKTQVNHHDKLVYIYTSGTTGLPKAAVISHS 365
Cdd:cd17634 177 KNVDDALNPNVTSVEHVIVLKRTGSDIDWQEGRDLwwRDLIAKASPEH-QPEAMNAEDPLFILYTSGTTGKPKGVLHTTG 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 366 RYLFIAA-GIHYTMGFQEEDIFYTPLPL-------YHTAGGIMCMGQSVLF-GSTVsirkkFSASNYFADCAKYNATIGQ 436
Cdd:cd17634 256 GYLVYAAtTMKYVFDYGPGDIYWCTADVgwvtghsYLLYGPLACGATTLLYeGVPN-----WPTPARMWQVVDKHGVNIL 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 437 YIGEMARYILATKPSEYDQKH---RVRLVFGNG--LRPQIWPQFVQRFNIAK--VGEFYGATEgnanimnhdnTVGAIGF 509
Cdd:cd17634 331 YTAPTAIRALMAAGDDAIEGTdrsSLRILGSVGepINPEAYEWYWKKIGKEKcpVVDTWWQTE----------TGGFMIT 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 510 VSRILPKIYPISIIRADPDTGEPIRDRNGlcqlcAPNEPGVfIGKIVKGNP----SREFLGYVDEK-ASAKKIVKDVFKH 584
Cdd:cd17634 401 PLPGAIELKAGSATRPVFGVQPAVVDNEG-----HPQPGGT-EGNLVITDPwpgqTRTLFGDHERFeQTYFSTFKGMYFS 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 585 GDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGvtIPH-TEGRAGMAAIYDPERE 663
Cdd:cd17634 475 GDGARR-------DEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVG--IPHaIKGQAPYAYVVLNHGV 545
|
570 580 590
....*....|....*....|....*....|....*....
gi 939619614 664 LDLDvfaaSLAKVLPAYARPQIIRLLTKVDLTGTFKLRK 702
Cdd:cd17634 546 EPSP----ELYAELRNWVRKEIGPLATPDVVHWVDSLPK 580
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
177-705 |
7.16e-22 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 101.47 E-value: 7.16e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG---Vit 253
Cdd:COG1020 477 TLHELFEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGaayV-- 554
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 254 PLintnlrGPS-----LLHSITVAHCSALIYGEDFLEAVTDVAKDLpanLTLfqfnnennnseTEKNIPQAKNLNALLTT 328
Cdd:COG1020 555 PL------DPAypaerLAYMLEDAGARLVLTQSALAARLPELGVPV---LAL-----------DALALAAEPATNPPVPV 614
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 329 ASyekpnktqvnhhDKLVY-IYTSGTTGLPKAAVISH---SRYLfiaAGIHYTMGFQEEDIF--YTPL-----------P 391
Cdd:COG1020 615 TP------------DDLAYvIYTSGSTGRPKGVMVEHralVNLL---AWMQRRYGLGPGDRVlqFASLsfdasvweifgA 679
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 392 LyhtaggimcmgqsvLFGSTVSIRKKFSASN--YFAD-CAKYNATIGQ----YIGEMARYILATKPSeydqkhrVRLVF- 463
Cdd:COG1020 680 L--------------LSGATLVLAPPEARRDpaALAElLARHRVTVLNltpsLLRALLDAAPEALPS-------LRLVLv 738
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 464 -GNGLRPQIWPQFVQRFNIAKVGEFYGATEgnanimnhdNTVGAIGF-VSRILPKIYPISIIRADPDTGEPIRDRNGlcQ 541
Cdd:COG1020 739 gGEALPPELVRRWRARLPGARLVNLYGPTE---------TTVDSTYYeVTPPDADGGSVPIGRPIANTRVYVLDAHL--Q 807
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 542 LCAPNEPG-VFIGkivkG-NPSReflGYV-DEKASAKKivkdvfkhgdmaFISGDLLVADEKgyLYfkdRTGDTFRW--- 615
Cdd:COG1020 808 PVPVGVPGeLYIG----GaGLAR---GYLnRPELTAER------------FVADPFGFPGAR--LY---RTGDLARWlpd 863
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 616 -------------K--GENVSTSEVEAQVSNVAGYKDTVVygVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAY 680
Cdd:COG1020 864 gnleflgraddqvKirGFRIELGEIEAALLQHPGVREAVV--VAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLP 941
|
570 580
....*....|....*....|....*
gi 939619614 681 ARPQIIRLLTKVDLTGTFKLRKVDL 705
Cdd:COG1020 942 PYMVPAAVVLLLPLPLTGNGKLDRL 966
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
183-708 |
2.51e-21 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 98.57 E-value: 2.51e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 183 ERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLIN---TN 259
Cdd:PRK06710 31 EQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNplyTE 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 260 LRGPSLLHS--ITVAHCSALIY-------GEDFLE--AVTDVAKDLP--ANLtLFQFNNENNNSEteknIPQAKNLNALL 326
Cdd:PRK06710 111 RELEYQLHDsgAKVILCLDLVFprvtnvqSATKIEhvIVTRIADFLPfpKNL-LYPFVQKKQSNL----VVKVSESETIH 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 327 TTASYEKPNKTQVN-----HHDKLVYIYTSGTTGLPKAAVISHSRYLF-IAAGIHYTMGFQE-EDIFYTPLPLYHTAGGI 399
Cdd:PRK06710 186 LWNSVEKEVNTGVEvpcdpENDLALLQYTGGTTGFPKGVMLTHKNLVSnTLMGVQWLYNCKEgEEVVLGVLPFFHVYGMT 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 400 MCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIgqYIGEMARYI-LATKP--SEYDQKHRVRLVFGNGLRPQIWPQFV 476
Cdd:PRK06710 266 AVMNLSIMQGYKMVLIPKFDMKMVFEAIKKHKVTL--FPGAPTIYIaLLNSPllKEYDISSIRACISGSAPLPVEVQEKF 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 477 QRFNIAKVGEFYGATEGN----ANIMNHDNTVGAIGfvsriLPkiYPISIIR-ADPDTGEPIRdrnglcqlcaPNEpgvf 551
Cdd:PRK06710 344 ETVTGGKLVEGYGLTESSpvthSNFLWEKRVPGSIG-----VP--WPDTEAMiMSLETGEALP----------PGE---- 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 552 IGKIVKGNPsREFLGYVDEKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSN 631
Cdd:PRK06710 403 IGEIVVKGP-QIMKGYWNKPEETAAVLQDGWLH------TGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYE 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 632 VAGYKDTVVYGVTIPHtEGRAGMAAIYDPE----RELDLDVFAaslAKVLPAYARPQIIRLLTKVDLTGTFK-LRKVDLQ 706
Cdd:PRK06710 476 HEKVQEVVTIGVPDPY-RGETVKAFVVLKEgtecSEEELNQFA---RKYLAAYKVPKVYEFRDELPKTTVGKiLRRVLIE 551
|
..
gi 939619614 707 KE 708
Cdd:PRK06710 552 EE 553
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
348-702 |
3.20e-21 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 97.45 E-value: 3.20e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKA------AVISHSRYLFIAAGIhytMGFQEEDIFYTPLPLYHTAGGIMCMGqSVLFGSTVSIRKKFSAS 421
Cdd:cd05929 131 LYSGGTTGRPKGikrglpGGPPDNDTLMAAALG---FGPGADSVYLSPAPLYHAAPFRWSMT-ALFMGGTLVLMEKFDPE 206
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 422 NYFADCAKYNATIGQYIGEMARYILA---TKPSEYDQKHRVRLVFGNGLRPqIWpqfVQRFNIA----KVGEFYGATEGN 494
Cdd:cd05929 207 EFLRLIERYRVTFAQFVPTMFVRLLKlpeAVRNAYDLSSLKRVIHAAAPCP-PW---VKEQWIDwggpIIWEYYGGTEGQ 282
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 495 A-NIMNHDNTVGAIGFVSRILpkiypisiiradpdTGE-PIRDRNGlcQLCAPNEPG-VFIgkivKGNPSREFLGYVDEK 571
Cdd:cd05929 283 GlTIINGEEWLTHPGSVGRAV--------------LGKvHILDEDG--NEVPPGEIGeVYF----ANGPGFEYTNDPEKT 342
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 572 ASAKKivkdvfKHGdmaFIS-GDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPHTE- 649
Cdd:cd05929 343 AAARN------EGG---WSTlGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGV--PDEEl 411
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 939619614 650 GRAGMAAIYDPERELDLDVFAASLA----KVLPAYARPQIIRLLTKVDLTGTFKLRK 702
Cdd:cd05929 412 GQRVHAVVQPAPGADAGTALAEELIaflrDRLSRYKCPRSIEFVAELPRDDTGKLYR 468
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
180-719 |
5.30e-21 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 97.72 E-value: 5.30e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVV--------SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGV 251
Cdd:PRK07529 29 ELLSRAAARHPDAPALSflldadplDRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGGEAAGI 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 252 ITPlINTNLRGPSLLHSITVAHCSALIY-----GEDFLEAVTDVAKDLPANLTLFQFNNENNNSETEKNIPQA------- 319
Cdd:PRK07529 109 ANP-INPLLEPEQIAELLRAAGAKVLVTlgpfpGTDIWQKVAEVLAALPELRTVVEVDLARYLPGPKRLAVPLirrkaha 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 320 --KNLNALLTTASYEKP-NKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTA 396
Cdd:PRK07529 188 riLDFDAELARQPGDRLfSGRPIGPDDVAAYFHTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPGDTVFCGLPLFHVN 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 397 G---GIMCM---GQSVLFGSTVSIRKKFSASNYFADCAKYNATigqyigemaryILATKPSEYDQKHRV----------R 460
Cdd:PRK07529 268 AllvTGLAPlarGAHVVLATPQGYRGPGVIANFWKIVERYRIN-----------FLSGVPTVYAALLQVpvdghdisslR 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 461 LVFGNG--LRPQIWPQFVQRFNIaKVGEFYGATEGNA----NIMNHDNTVGAIGfvsriLPKIYP-ISIIRADPDtGEPI 533
Cdd:PRK07529 337 YALCGAapLPVEVFRRFEAATGV-RIVEGYGLTEATCvssvNPPDGERRIGSVG-----LRLPYQrVRVVILDDA-GRYL 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 534 RDrnglcqlCAPNEPGVfigkIVKGNPSrEFLGYVDEKASakkivKDVFKHGDMaFISGDLLVADEKGYLYFKDRTGDTF 613
Cdd:PRK07529 410 RD-------CAVDEVGV----LCIAGPN-VFSGYLEAAHN-----KGLWLEDGW-LNTGDLGRIDADGYFWLTGRAKDLI 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 614 RWKGENVSTSEVEA------QVSNVA--GYKDT------VVYgVTIphtegRAGMAAiyDPEReldLDVFAAslAKVLPA 679
Cdd:PRK07529 472 IRGGHNIDPAAIEEallrhpAVALAAavGRPDAhagelpVAY-VQL-----KPGASA--TEAE---LLAFAR--DHIAER 538
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 939619614 680 YARPQIIRLLTKVDLTGTFKLRKVDLQKegydpNAIKDAL 719
Cdd:PRK07529 539 AAVPKHVRILDALPKTAVGKIFKPALRR-----DAIRRVL 573
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
183-707 |
6.04e-21 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 97.16 E-value: 6.04e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 183 ERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRG 262
Cdd:PRK07786 24 ARHALMQPDAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTP 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 263 PSLlhSITVAHCSA-LIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSETEknipqakNLNALLTTASYEKPNKTQVNH 341
Cdd:PRK07786 104 PEI--AFLVSDCGAhVVVTEAALAPVATAVRDIVPLLSTVVVAGGSSDDSVL-------GYEDLLAEAGPAHAPVDIPND 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 342 HDKLVyIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMG-FQEEDIFYTPLPLYHTAgGIMCMGQSVLFGSTVSIR--KKF 418
Cdd:PRK07786 175 SPALI-MYTSGTTGRPKGAVLTHANLTGQAMTCLRTNGaDINSDVGFVGVPLFHIA-GIGSMLPGLLLGAPTVIYplGAF 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 419 SASNYFADCAKYNATIGQYIGEMARYILAtkpseyDQKHRVR----LVFGNGLRP---QIWPQFVQRFNIAKVGEFYGAT 491
Cdd:PRK07786 253 DPGQLLDVLEAEKVTGIFLVPAQWQAVCA------EQQARPRdlalRVLSWGAAPasdTLLRQMAATFPEAQILAAFGQT 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 492 EGN--ANIMNHDNTVGAIGFVSRILPKiypISIIRADPDTGEpirdrnglcqlCAPNEpgvfIGKIVKGNPSReFLGYVD 569
Cdd:PRK07786 327 EMSpvTCMLLGEDAIRKLGSVGKVIPT---VAARVVDENMND-----------VPVGE----VGEIVYRAPTL-MSGYWN 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 570 E-KASAkkivkDVFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEaqvsNV-AGYKDTV-VYGVTIP 646
Cdd:PRK07786 388 NpEATA-----EAFAGG--WFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVE----NVlASHPDIVeVAVIGRA 456
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 939619614 647 H---TEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQK 707
Cdd:PRK07786 457 DekwGEVPVAVAAVRNDDAALTLEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRE 520
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
177-643 |
9.15e-21 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 96.42 E-value: 9.15e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVV--SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITP 254
Cdd:cd05923 2 TVFEMLRRAASRAPDACAIAdpARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 LINTNLRGPSLLHSITVAHCSALIygedfleaVTDVAKDLPANLTLFqfnnennnseteKNIPQAKNLNALLTTASYEKP 334
Cdd:cd05923 82 LINPRLKAAELAELIERGEMTAAV--------IAVDAQVMDAIFQSG------------VRVLALSDLVGLGEPESAGPL 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 NKT-QVNHHDKLVYIYTSGTTGLPKAAVISH----SRYLFIAAGIHYTMGFQEEDIFYtpLPLYHTAGGIMCMGQSVLFG 409
Cdd:cd05923 142 IEDpPREPEQPAFVFYTSGTTGLPKGAVIPQraaeSRVLFMSTQAGLRHGRHNVVLGL--MPLYHVIGFFAVLVAALALD 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 410 STVSIRKKFSA------------SNYFADCAKYNATIGQYIGEmaryilATKPSEYDQkhrvrLVFGNGLRPQiwpQFVQ 477
Cdd:cd05923 220 GTYVVVEEFDPadalklieqervTSLFATPTHLDALAAAAEFA------GLKLSSLRH-----VTFAGATMPD---AVLE 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 478 RFNIAKVGEF---YGATEGNANIMNHD---NTVGAIGFVSRIlpKIYPI----SIIRADPDTGEPIRDRNGlcqlcapne 547
Cdd:cd05923 286 RVNQHLPGEKvniYGTTEAMNSLYMRDartGTEMRPGFFSEV--RIVRIggspDEALANGEEGELIVAAAA--------- 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 548 pgvfigkivkgnpSREFLGYVDE-KASAKKIVKDVFKHGDMAFISGDllvadekGYLYFKDRTGDTFRWKGENVSTSEVE 626
Cdd:cd05923 355 -------------DAAFTGYLNQpEATAKKLQDGWYRTGDVGYVDPS-------GDVRILGRVDDMIISGGENIHPSEIE 414
|
490
....*....|....*..
gi 939619614 627 AQVSNVAGYKDTVVYGV 643
Cdd:cd05923 415 RVLSRHPGVTEVVVIGV 431
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
181-705 |
3.17e-20 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 94.16 E-value: 3.17e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 181 VFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNL 260
Cdd:cd17645 3 LFEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDY 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 261 RGpsllhsitvahcsaliygedflEAVTDVAKDLPANLtlfqfnnennnseteknipqaknlnaLLTTAsyekpnktqvn 340
Cdd:cd17645 83 PG----------------------ERIAYMLADSSAKI--------------------------LLTNP----------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 341 hhDKLVY-IYTSGTTGLPKAAVISHSRYLFIAAGIH--YTMGFQEEDIFYTPLPLyhtAGGIMCMGQSVLFGSTV----- 412
Cdd:cd17645 104 --DDLAYvIYTSGSTGLPKGVMIEHHNLVNLCEWHRpyFGVTPADKSLVYASFSF---DASAWEIFPHLTAGAALhvvps 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 413 SIRKKFSASNYFadCAKYNATIGQYIGEMARYILATKpseyDQKHRVRLVFGNGLRpqiwpQFVQrfNIAKVGEFYGATE 492
Cdd:cd17645 179 ERRLDLDALNDY--FNQEGITISFLPTGAAEQFMQLD----NQSLRVLLTGGDKLK-----KIER--KGYKLVNNYGPTE 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 493 gnanimnhdNTVGAIGFvsRILPKIYPISIIRADPDTGEPIRDR-NGLCQLCAPNEPGVFIGKIVKGNPSREFLgyvdek 571
Cdd:cd17645 246 ---------NTVVATSF--EIDKPYANIPIGKPIDNTRVYILDEaLQLQPIGVAGELCIAGEGLARGYLNRPEL------ 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 572 aSAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTipHTEGR 651
Cdd:cd17645 309 -TAEKFIVHPFVPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKE--DADGR 385
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 939619614 652 AGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDL 705
Cdd:cd17645 386 KYLVAYVTAPEEIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
185-702 |
5.27e-20 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 93.92 E-value: 5.27e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 185 NVQAHPDKVAV-VSET-QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRG 262
Cdd:PRK13390 6 HAQIAPDRPAViVAETgEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTA 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 263 PSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLfqfnnennnsetEKNIPQAKNLNALLTTAS---YEKPNKTqv 339
Cdd:PRK13390 86 PEADYIVGDSGARVLVASAALDGLAAKVGADLPLRLSF------------GGEIDGFGSFEAALAGAGprlTEQPCGA-- 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 340 nhhdklVYIYTSGTTGLPKA---------------AVISHSRYLFiaagihytmGFQEEDIFYTPLPLYHTAGGIMCmGQ 404
Cdd:PRK13390 152 ------VMLYSSGTTGFPKGiqpdlpgrdvdapgdPIVAIARAFY---------DISESDIYYSSAPIYHAAPLRWC-SM 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 405 SVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEM------------ARY--------ILATKPSEYDQKHRVRlvfg 464
Cdd:PRK13390 216 VHALGGTVVLAKRFDAQATLGHVERYRITVTQMVPTMfvrllkldadvrTRYdvsslravIHAAAPCPVDVKHAMI---- 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 465 NGLRPQIWpqfvqrfniakvgEFYGATEGNA-NIMNHDNTVGAIGFVSR-ILPKIYpisiiradpdtgepIRDRNGlcql 542
Cdd:PRK13390 292 DWLGPIVY-------------EYYSSTEAHGmTFIDSPDWLAHPGSVGRsVLGDLH--------------ICDDDG---- 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 543 capNE-PGVFIGKIVKGNPSREFLGYVDEKASAKKivkdvfKHGDMAFIS--GDLLVADEKGYLYFKDRTGDTFRWKGEN 619
Cdd:PRK13390 341 ---NElPAGRIGTVYFERDRLPFRYLNDPEKTAAA------QHPAHPFWTtvGDLGSVDEDGYLYLADRKSFMIISGGVN 411
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 620 VSTSEVEAQVSNVAGYKDTVVYGVTIPHTeGRAGMAAIY-----DPERELDLDVFAASLAKVlPAYARPQIIRLLTKVDL 694
Cdd:PRK13390 412 IYPQETENALTMHPAVHDVAVIGVPDPEM-GEQVKAVIQlvegiRGSDELARELIDYTRSRI-AHYKAPRSVEFVDELPR 489
|
....*...
gi 939619614 695 TGTFKLRK 702
Cdd:PRK13390 490 TPTGKLVK 497
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
201-627 |
5.34e-20 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 93.60 E-value: 5.34e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 201 RWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLINtnlrgpsllhsitvahcsalIY 279
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGaVTNPILP--------------------FF 60
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 280 GEDFLEAVTDVAkdlpanltlfqfnnennnseteknipQAKnlnALLTTASYEKPNKTQVNhhDKLVYI-YTSGTTGLPK 358
Cdd:cd05903 61 REHELAFILRRA--------------------------KAK---VFVVPERFRQFDPAAMP--DAVALLlFTSGTTGEPK 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 359 AAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSAsnyfADCAKYNATIGQYI 438
Cdd:cd05903 110 GVMHSHNTLSASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIWDP----DKALALMREHGVTF 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 439 GEMARYILA--TKPSEYDQKHRVRL---VFGNGLRPQIWPQFVQRFNIAKVGEFYGATEgnanimnHDNTVGAI--GFVS 511
Cdd:cd05903 186 MMGATPFLTdlLNAVEEAGEPLSRLrtfVCGGATVPRSLARRAAELLGAKVCSAYGSTE-------CPGAVTSItpAPED 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 512 RIL---PKIYPISIIRADPDTGepirdrnglcqlcAPNEPGVfIGKIVKGNPSReFLGYVDEKASAKKIVKDVFkhgdma 588
Cdd:cd05903 259 RRLytdGRPLPGVEIKVVDDTG-------------ATLAPGV-EGELLSRGPSV-FLGYLDRPDLTADAAPEGW------ 317
|
410 420 430
....*....|....*....|....*....|....*....
gi 939619614 589 FISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEA 627
Cdd:cd05903 318 FRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVED 356
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
186-704 |
7.79e-20 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 93.08 E-value: 7.79e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 186 VQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGviTPLINTNLRGPSl 265
Cdd:cd05945 1 AAANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAG--HAYVPLDASSPA- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 266 lhsitvahcsaliygeDFLEAVTDVAKdlPAnltlfqfnnennnseteknipqaknlnALLTTAsyekpnktqvnhhDKL 345
Cdd:cd05945 78 ----------------ERIREILDAAK--PA---------------------------LLIADG-------------DDN 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 346 VYI-YTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFyTPLPLYHTAGGIMCMGQSVLFGST-VSIRKkfSASNY 423
Cdd:cd05945 100 AYIiFTSGSTGRPKGVQISHDNLVSFTNWMLSDFPLGPGDVF-LNQAPFSFDLSVMDLYPALASGATlVPVPR--DATAD 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 424 FADCAKY--NATIGQYIGEMARYILATKPSEYDQKH--RVRLVF--GNGLRPQIWPQFVQRFNIAKVGEFYGATEGNAni 497
Cdd:cd05945 177 PKQLFRFlaEHGITVWVSTPSFAAMCLLSPTFTPESlpSLRHFLfcGEVLPHKTARALQQRFPDARIYNTYGPTEATV-- 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 498 mnhdnTVGAIGFVSRILPKIYPISIIRADPDTGEPIRDRNGlcqlcAPNEPGVfIGKIVKGNPSReFLGYVDEkasAKKI 577
Cdd:cd05945 255 -----AVTYIEVTPEVLDGYDRLPIGYAKPGAKLVILDEDG-----RPVPPGE-KGELVISGPSV-SKGYLNN---PEKT 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 578 VKDVFKH-GDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVygVTIPHTEGRAGMAA 656
Cdd:cd05945 320 AAAFFPDeGQRAYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVV--VPKYKGEKVTELIA 397
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 939619614 657 IYDPERELD---LDVFAASLAKVLPAYARPQIIRLLTKVDLTGTfklRKVD 704
Cdd:cd05945 398 FVVPKPGAEaglTKAIKAELAERLPPYMIPRRFVYLDELPLNAN---GKID 445
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
179-684 |
1.69e-19 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 92.80 E-value: 1.69e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 179 ADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVItpLINT 258
Cdd:PRK07470 10 AHFLRQAARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAV--WVPT 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 259 NLR--GPSLLHSITVAHCSALIYGEDF---LEAVTDVAKDLPANLTLfqfnnennnseteKNIPQAKNLNALLTTASYEK 333
Cdd:PRK07470 88 NFRqtPDEVAYLAEASGARAMICHADFpehAAAVRAASPDLTHVVAI-------------GGARAGLDYEALVARHLGAR 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 334 PNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGiH---YTMGFQEEDIFYTPLPLYHTAgGIMCMGQ------ 404
Cdd:PRK07470 155 VANAAVDHDDPCWFFFTSGTTGRPKAAVLTHGQMAFVITN-HladLMPGTTEQDASLVVAPLSHGA-GIHQLCQvargaa 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 405 SVLFGStvsirKKFSASNYFADCAKYNAT-----------------IGQYIGEMARYILATKPSEY--DQKhRVRLVFGN 465
Cdd:PRK07470 233 TVLLPS-----ERFDPAEVWALVERHRVTnlftvptilkmlvehpaVDRYDHSSLRYVIYAGAPMYraDQK-RALAKLGK 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 466 GLrpqiwpqfVQRFNIAKVgefygatEGNANIMN---HD------NTVGAIGFvsrilpkiypisiiradPDTGE--PIR 534
Cdd:PRK07470 307 VL--------VQYFGLGEV-------TGNITVLPpalHDaedgpdARIGTCGF-----------------ERTGMevQIQ 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 535 DRNGlcQLCAPNEPG--VFIGKIVkgnpsreFLGYVDE-KASAKkivkdVFKHGdmAFISGDLLVADEKGYLYFKDRTGD 611
Cdd:PRK07470 355 DDEG--RELPPGETGeiCVIGPAV-------FAGYYNNpEANAK-----AFRDG--WFRTGDLGHLDARGFLYITGRASD 418
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 939619614 612 TFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPhTEGRAGMAAIYDPE-RELDLDVFAASLAKVLPAYARPQ 684
Cdd:PRK07470 419 MYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDP-VWGEVGVAVCVARDgAPVDEAELLAWLDGKVARYKLPK 491
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
182-684 |
3.33e-19 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 91.48 E-value: 3.33e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 182 FERNVQAHPDKVAVVSET---QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLiN 257
Cdd:PRK07514 6 FDALRAAFADRDAPFIETpdgLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGaVFLPL-N 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 258 TNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPAnltlfqfnnennnsetekniPQAKNLNA-----LLTTASYE 332
Cdd:PRK07514 85 TAYTLAELDYFIGDAEPALVVCDPANFAWLSKIAAAAGA--------------------PHVETLDAdgtgsLLEAAAAA 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 333 KPNKTQVNHH-DKLVYI-YTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAG------GIMCMGQ 404
Cdd:PRK07514 145 PDDFETVPRGaDDLAAIlYTSGTTGRSKGAMLSHGNLLSNALTLVDYWRFTPDDVLIHALPIFHTHGlfvatnVALLAGA 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 405 SVLFgstvsirkkfsasnyfadCAKYNAtiGQYIGEMARyilATK----PSEY-----------DQKHRVRLvFGNG--- 466
Cdd:PRK07514 225 SMIF------------------LPKFDP--DAVLALMPR---ATVmmgvPTFYtrllqeprltrEAAAHMRL-FISGsap 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 467 LRPQIWPQFVQRFNIAkVGEFYGATEGNANIMN---HDNTVGAIGFVsriLPKIypiSIIRADPDTGEPirdrnglcqlC 543
Cdd:PRK07514 281 LLAETHREFQERTGHA-ILERYGMTETNMNTSNpydGERRAGTVGFP---LPGV---SLRVTDPETGAE----------L 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 544 APNEpgvfIGKI-VKGnPSReFLGY--VDEKaSAKKIVKDVFkhgdmaFISGDLLVADEKGYLYFKDRTGDTFRWKGENV 620
Cdd:PRK07514 344 PPGE----IGMIeVKG-PNV-FKGYwrMPEK-TAEEFRADGF------FITGDLGKIDERGYVHIVGRGKDLIISGGYNV 410
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 621 STSEVEAQVSNVAGYKDTVVYGVtiPHTE-GRAGMAAIY-DPERELDLDVFAASLAKVLPAYARPQ 684
Cdd:PRK07514 411 YPKEVEGEIDELPGVVESAVIGV--PHPDfGEGVTAVVVpKPGAALDEAAILAALKGRLARFKQPK 474
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
202-683 |
1.83e-18 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 88.81 E-value: 1.83e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 202 WTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVIT-PLINTNlrGPSLLHSItVAHCSALIyg 280
Cdd:cd05907 6 ITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPvPIYPTS--SAEQIAYI-LNDSEAKA-- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 281 edfleAVTDVAKDLpANLtlfqfnnennnseteknipqaknlnallttasyekpnktqvnhhdklvyIYTSGTTGLPKAA 360
Cdd:cd05907 81 -----LFVEDPDDL-ATI-------------------------------------------------IYTSGTTGRPKGV 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 361 VISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIrkKFSASNYFADCAK----------- 429
Cdd:cd05907 106 MLSHRNILSNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYF--ASSAETLLDDLSEvrptvflavpr 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 430 -----YNATIGQYIGEMARYILATKPSEydqkhRVRLVFGNG--LRPQIwPQFVQRFNIaKVGEFYGATEGNANI-MNH- 500
Cdd:cd05907 184 vwekvYAAIKVKAVPGLKRKLFDLAVGG-----RLRFAASGGapLPAEL-LHFFRALGI-PVYEGYGLTETSAVVtLNPp 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 501 -DNTVGAIGfvsrilpKIYPISIIRADPDtgepirdrnglcqlcapnepgvfiGKI-VKGNPSreFLGYV-DEKASAKKI 577
Cdd:cd05907 257 gDNRIGTVG-------KPLPGVEVRIADD------------------------GEIlVRGPNV--MLGYYkNPEATAEAL 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 578 VKD-VFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRW-KGENVSTSEVEAQVSNVAGYKDTVVYGvtiphtEGRAGMA 655
Cdd:cd05907 304 DADgWLHTGDLGEI-------DEDGFLHITGRKKDLIITsGGKNISPEPIENALKASPLISQAVVIG------DGRPFLV 370
|
490 500
....*....|....*....|....*...
gi 939619614 656 AIYDPerelDLDVFAASLAKVLPAYARP 683
Cdd:cd05907 371 ALIVP----DPEALEAWAEEHGIAYTDV 394
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
176-701 |
2.23e-18 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 88.97 E-value: 2.23e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 176 YTVADVFERNVQA-HPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITP 254
Cdd:cd05959 3 YNAATLVDLNLNEgRGDKTAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 LINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLtlfqfnNENNNSETEKNIPQAKNLNALLTTASYE-K 333
Cdd:cd05959 83 PVNTLLTPDDYAYYLEDSRARVVVVSGELAPVLAAALTKSEHTL------VVLIVSGGAGPEAGALLLAELVAAEAEQlK 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 334 PNKTQVNhhDKLVYIYTSGTTGLPKAAVISHSRylFIAAGIHY---TMGFQEEDIFYTPLPLYHTAGgimcMGQSVLF-- 408
Cdd:cd05959 157 PAATHAD--DPAFWLYSSGSTGRPKGVVHLHAD--IYWTAELYarnVLGIREDDVCFSAAKLFFAYG----LGNSLTFpl 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 409 ---GSTVSIRKKFSASNYFADCAKYNATIgqYIGEMARY--ILAT-KPSEYDQKhRVRLVF--GNGLRPQIWPQFVQRFN 480
Cdd:cd05959 229 svgATTVLMPERPTPAAVFKRIRRYRPTV--FFGVPTLYaaMLAApNLPSRDLS-SLRLCVsaGEALPAEVGERWKARFG 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 481 IAKVgEFYGATEgnaniMNH---DNTVGAI--GFVSRILPKiYPISIIraDPDtGEPIRDrnglcqlcapNEPGVFigkI 555
Cdd:cd05959 306 LDIL-DGIGSTE-----MLHiflSNRPGRVryGTTGKPVPG-YEVELR--DED-GGDVAD----------GEPGEL---Y 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 556 VKGNPSREflGYVDEKASAKKivkdVFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGY 635
Cdd:cd05959 363 VRGPSSAT--MYWNNRDKTRD----TFQGE--WTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAV 434
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 636 KDTVVYGVtiPHTEGRAGMAAIYDPER-----ELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGT-----FKLR 701
Cdd:cd05959 435 LEAAVVGV--EDEDGLTKPKAFVVLRPgyedsEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATgkiqrFKLR 508
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
177-707 |
5.68e-18 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 87.53 E-value: 5.68e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGyKKGDVVALLLENRAEYVATWLGLSKIGVITPLI 256
Cdd:PRK07638 2 GITKEYKKHASLQPNKIAIKENDRVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVakdlpanltlfqfnnennnSETEKNIPQAKNlnalltTASYEKPNK 336
Cdd:PRK07638 81 DIKWKQDELKERLAISNADMIVTERYKLNDLPDE-------------------EGRVIEIDEWKR------MIEKYLPTY 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 337 TQVN--HHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTA---GGImcmgqSVLF-GS 410
Cdd:PRK07638 136 APIEnvQNAPFYMGFTSGSTGKPKAFLRAQQSWLHSFDCNVHDFHMKREDSVLIAGTLVHSLflyGAI-----STLYvGQ 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 411 TVSIRKKFSASNYFADCAKYNATIgqyigemaryiLATKPSEYDQKHRVRLVFGNGLRpqI------WP-----QFVQRF 479
Cdd:PRK07638 211 TVHLMRKFIPNQVLDKLETENISV-----------MYTVPTMLESLYKENRVIENKMK--IissgakWEaeakeKIKNIF 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 480 NIAKVGEFYGATEgnanimnhdntvgaIGFVSRILP---KIYPISIIRadPDTGEPIRDRNGLCQLCAPNEpgvfIGKIV 556
Cdd:PRK07638 278 PYAKLYEFYGASE--------------LSFVTALVDeesERRPNSVGR--PFHNVQVRICNEAGEEVQKGE----IGTVY 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 557 KGNPSReFLGYVDEKASAKKI-VKDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGY 635
Cdd:PRK07638 338 VKSPQF-FMGYIIGGVLARELnADGWMTVRDVGYE-------DEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAV 409
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 939619614 636 KDTVVYGVTIPHTeGRAGMAAIYDPERELDLDVFaasLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQK 707
Cdd:PRK07638 410 DEIVVIGVPDSYW-GEKPVAIIKGSATKQQLKSF---CLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKS 477
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
203-700 |
5.71e-18 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 87.15 E-value: 5.71e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 203 TFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYGED 282
Cdd:cd05935 3 TYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGSE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 283 fLEavtDVAkdlpanltlfqfnnennnsetekNIPqaknlnallttasyekpnktqvnhhdklvyiYTSGTTGLPKAAVI 362
Cdd:cd05935 83 -LD---DLA-----------------------LIP-------------------------------YTSGTTGLPKGCMH 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 363 SHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMA 442
Cdd:cd05935 105 THFSAAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMARWDRETALELIEKYKVTFWTNIPTML 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 443 RYILAT-KPSEYDQKHRVrlVFGNG---LRPQIWPQFVQRFNIAKVgEFYGATEGNANImnHDNTVGAIGFVSRILPkIY 518
Cdd:cd05935 185 VDLLATpEFKTRDLSSLK--VLTGGgapMPPAVAEKLLKLTGLRFV-EGYGLTETMSQT--HTNPPLRPKLQCLGIP-*F 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 519 PISIIRADPDTGEPIrdrnglcqlcAPNEpgvfIGKIVKGNPSReFLGYVD-EKASAKKIVKDvfkHGDMAFISGDLLVA 597
Cdd:cd05935 259 GVDARVIDIETGREL----------PPNE----VGEIVVRGPQI-FKGYWNrPEETEESFIEI---KGRRFFRTGDLGYM 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 598 DEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYDPER--ELDLDVFAASLAK 675
Cdd:cd05935 321 DEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYrgKVTEEDIIEWARE 400
|
490 500
....*....|....*....|....*
gi 939619614 676 VLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd05935 401 QMAAYKYPREVEFVDELPRSASGKI 425
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
166-705 |
7.43e-18 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 87.38 E-value: 7.43e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 166 RYTKRHERLNYTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLG 245
Cdd:cd05920 5 RYRAAGYWQDEPLGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 246 LSKIGVITPLINTNLRGPSLLHSITVAHCSALI-----YGEDFLEAVTDVAKDLPAnLTLFQFnnennnseteknipqak 320
Cdd:cd05920 85 LLRLGAVPVLALPSHRRSELSAFCAHAEAVAYIvpdrhAGFDHRALARELAESIPE-VALFLL----------------- 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 321 nlnallttasyekpnktqvnhhdklvyiyTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAgGIM 400
Cdd:cd05920 147 -----------------------------SGGTTGTPKLIPRTHNDYAYNVRASAEVCGLDQDTVYLAVLPAAHNF-PLA 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 401 CMGQ--SVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYIL--ATKPSEYDQKHRVRLVFGNGLRPQIWPQFV 476
Cdd:cd05920 197 CPGVlgTLLAGGRVVLAPDPSPDAAFPLIEREGVTVTALVPALVSLWLdaAASRRADLSSLRLLQVGGARLSPALARRVP 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 477 QRFNiAKVGEFYGATEGNANIMNHD-------NTVGaigfvsrilpkiYPISiiradPDTGEPIRDRNGlcQLCAPNEPG 549
Cdd:cd05920 277 PVLG-CTLQQVFGMAEGLLNYTRLDdpdeviiHTQG------------RPMS-----PDDEIRVVDEEG--NPVPPGEEG 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 550 VFigkIVKGnpSREFLGYVD-EKASAKKIVKDVFkhgdmaFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQ 628
Cdd:cd05920 337 EL---LTRG--PYTIRGYYRaPEHNARAFTPDGF------YRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENL 405
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 939619614 629 VSNVAGYKDTVVygVTIPHTE-GRAGMAAIYDPERELDLDVFAASL-AKVLPAYARPQIIRLLTKVDLTGTFKLRKVDL 705
Cdd:cd05920 406 LLRHPAVHDAAV--VAMPDELlGERSCAFVVLRDPPPSAAQLRRFLrERGLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
177-608 |
1.14e-17 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 86.94 E-value: 1.14e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLG-LSKIGVITP 254
Cdd:PRK08314 11 SLFHNLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYYAiLRANAVVVP 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 lINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLP-ANLTLFQFNNENNNS---------ETEKNIPQAKNLNA 324
Cdd:PRK08314 91 -VNPMNREEELAHYVTDSGARVAIVGSELAPKVAPAVGNLRlRHVIVAQYSDYLPAEpeiavpawlRAEPPLQALAPGGV 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 325 LL---TTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMC 401
Cdd:PRK08314 170 VAwkeALAAGLAPPPHTAGPDDLAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLAVLPLFHVTGMVHS 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 402 MGQSVLFGSTVSI--RKKFSASnyfADC-AKYNATIGQYIGEMARYILAT-KPSEYDQKhRVRLVFGNGlrpQIWPQFV- 476
Cdd:PRK08314 250 MNAPIYAGATVVLmpRWDREAA---ARLiERYRVTHWTNIPTMVVDFLASpGLAERDLS-SLRYIGGGG---AAMPEAVa 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 477 ----QRFNIAKVgEFYGATEGNANImnHDNT----------VGAIGFVSRILpkiypisiiraDPDTGEPIrdrnglcql 542
Cdd:PRK08314 323 erlkELTGLDYV-EGYGLTETMAQT--HSNPpdrpklqclgIPTFGVDARVI-----------DPETLEEL--------- 379
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 543 cAPNEpgvfIGKIVKGNPSReFLGYVD-EKASAKKIV----KDVFKHGDMAFIsgdllvaDEKGYLYFKDR 608
Cdd:PRK08314 380 -PPGE----VGEIVVHGPQV-FKGYWNrPEATAEAFIeidgKRFFRTGDLGRM-------DEEGYFFITDR 437
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
180-700 |
1.48e-17 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 86.18 E-value: 1.48e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPL--- 255
Cdd:cd17646 2 ALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGaAYLPLdpg 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 -----INTNLR--GPSLLhsITVAHCSALIYGEDFLEAVTDVAKDLPAnltlfqfnnennnseteKNIPQAknlnalltt 328
Cdd:cd17646 82 ypadrLAYMLAdaGPAVV--LTTADLAARLPAGGDVALLGDEALAAPP-----------------ATPPLV--------- 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 329 asyekpnktqVNHHDKLVY-IYTSGTTGLPKAAVISHsrylfiaAGI-HYTMGFQEEdifytpLPLYHtaggimcmGQSV 406
Cdd:cd17646 134 ----------PPRPDNLAYvIYTSGSTGRPKGVMVTH-------AGIvNRLLWMQDE------YPLGP--------GDRV 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 407 LF----GSTVSIRKKF-----SASNYFA------DCAKYNATI---------------GQYIGEMARYILATkpseydqk 456
Cdd:cd17646 183 LQktplSFDVSVWELFwplvaGARLVVArpgghrDPAYLAALIrehgvttchfvpsmlRVFLAEPAAGSCAS-------- 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 457 hrVRLVF--GNGLRPQIWPQFVQRFNIAKVgEFYGATEGnanimnhdnTVGAIGFVSRILPKIYPISIIRADPDTGEPIR 534
Cdd:cd17646 255 --LRRVFcsGEALPPELAARFLALPGAELH-NLYGPTEA---------AIDVTHWPVRGPAETPSVPIGRPVPNTRLYVL 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 535 DRNGlcqlcAPNEPGVfIGKIVKGNPSREfLGYVDEKA-SAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTF 613
Cdd:cd17646 323 DDAL-----RPVPVGV-PGELYLGGVQLA-RGYLGRPAlTAERFVPDPFGPGSRMYRTGDLARWRPDGALEFLGRSDDQV 395
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 614 RWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGR-AGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKV 692
Cdd:cd17646 396 KIRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARlVGYVVPAAGAAGPDTAALRAHLAERLPEYMVPAAFVVLDAL 475
|
....*...
gi 939619614 693 DLTGTFKL 700
Cdd:cd17646 476 PLTANGKL 483
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
181-627 |
2.68e-17 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 85.80 E-value: 2.68e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 181 VFERnVQAHPDKVAVVS-ETQR-WTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINT 258
Cdd:PLN02246 29 CFER-LSEFSDRPCLIDgATGRvYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANP 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 259 nLRGPSLLHS----------ITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFqFnnennnseteknipqaknlnALLTT 328
Cdd:PLN02246 108 -FYTPAEIAKqakasgakliITQSCYVDKLKGLAEDDGVTVVTIDDPPEGCLH-F--------------------SELTQ 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 329 ASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAA----GIHYTMGFQEEDIFYTPLPLYHtaggIMCMgQ 404
Cdd:PLN02246 166 ADENELPEVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAqqvdGENPNLYFHSDDVILCVLPMFH----IYSL-N 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 405 SVLF-----GSTVSIRKKFSASNYFADCAKYNATIGQYIGEMaryILATKPSEYDQKH---RVRLVFgNGLRP---QIWP 473
Cdd:PLN02246 241 SVLLcglrvGAAILIMPKFEIGALLELIQRHKVTIAPFVPPI---VLAIAKSPVVEKYdlsSIRMVL-SGAAPlgkELED 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 474 QFVQRFNIAKVGEFYGATE-GNANIMN-------HDNTVGAIGFVSRILP-KIypisiirADPDTGEPIRdRNGLCQLCA 544
Cdd:PLN02246 317 AFRAKLPNAVLGQGYGMTEaGPVLAMClafakepFPVKSGSCGTVVRNAElKI-------VDPETGASLP-RNQPGEICI 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 545 PNEpgvfigKIVKGnpsreFLGyvDEKASAKKIVKDVFKH-GDMAFISGDllvaDEkgyLYFKDRTGDTFRWKGENVSTS 623
Cdd:PLN02246 389 RGP------QIMKG-----YLN--DPEATANTIDKDGWLHtGDIGYIDDD----DE---LFIVDRLKELIKYKGFQVAPA 448
|
....
gi 939619614 624 EVEA 627
Cdd:PLN02246 449 ELEA 452
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
201-626 |
3.11e-17 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 85.60 E-value: 3.11e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 201 RWTFRQVNEHANKVANVL-QAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIY 279
Cdd:cd05928 41 KWSFRELGSLSRKAANVLsGACGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVT 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 280 GEDFLEAVTDVAKDLPANLTlfqfnnenNNSETEKNIPQAKNLNALLTTASyEKPNKTQVNHHDKLVYIYTSGTTGLPKA 359
Cdd:cd05928 121 SDELAPEVDSVASECPSLKT--------KLLVSEKSRDGWLNFKELLNEAS-TEHHCVETGSQEPMAIYFTSGTTGSPKM 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 360 AVISHSRY-LFIAAGIHYTMGFQEEDIFYTplplYHTAGGIMCMGQSV----LFGSTVSIRK--KFSASNYFADCAKYNA 432
Cdd:cd05928 192 AEHSHSSLgLGLKVNGRYWLDLTASDIMWN----TSDTGWIKSAWSSLfepwIQGACVFVHHlpRFDPLVILKTLSSYPI 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 433 TIGQYIGEMARYILATKPSEYDQKH-RVRLVFGNGLRPQIWPQFVQRFNIaKVGEFYGATEgnanimnhdntVGAIGFVS 511
Cdd:cd05928 268 TTFCGAPTVYRMLVQQDLSSYKFPSlQHCVTGGEPLNPEVLEKWKAQTGL-DIYEGYGQTE-----------TGLICANF 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 512 RILpKIYPISIIRADPDTGEPIRDRNGlcQLCAPNEPGVFIGKIVKGNPSREFLGYVDEKASAKKIVKDVFkhgdmaFIS 591
Cdd:cd05928 336 KGM-KIKPGSMGKASPPYDVQIIDDNG--NVLPPGTEGDIGIRVKPIRPFGLFSGYVDNPEKTAATIRGDF------YLT 406
|
410 420 430
....*....|....*....|....*....|....*
gi 939619614 592 GDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVE 626
Cdd:cd05928 407 GDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVE 441
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
343-707 |
4.05e-17 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 85.08 E-value: 4.05e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 343 DKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVsirkkfsasn 422
Cdd:cd05909 148 DPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKV---------- 217
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 423 YFAdcakYNATIGQYIGEMARYILAT-------------KPSEYDQKHRVRLVF--GNGLRPQIWPQFVQRFNIAKVgEF 487
Cdd:cd05909 218 VFH----PNPLDYKKIPELIYDKKATillgtptflrgyaRAAHPEDFSSLRLVVagAEKLKDTLRQEFQEKFGIRIL-EG 292
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 488 YGATEGNANI-MNHDNTVGAIGFVSRILPKIyPISIIraDPDTGEPIrdrnglcqlcAPNEPGVFigkIVKGnPSReFLG 566
Cdd:cd05909 293 YGTTECSPVIsVNTPQSPNKEGTVGRPLPGM-EVKIV--SVETHEEV----------PIGEGGLL---LVRG-PNV-MLG 354
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 567 YVDEKAsakkivKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGyKDTVVYGVTIP 646
Cdd:cd05909 355 YLNEPE------LTSFAFGDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEILP-EDNEVAVVSVP 427
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 939619614 647 HteGRAG--MAAIYDPErELDLDVFAASLAKV-LPAYARPQIIRLLTKVDLTGTFKLRKVDLQK 707
Cdd:cd05909 428 D--GRKGekIVLLTTTT-DTDPSSLNDILKNAgISNLAKPSYIHQVEEIPLLGTGKPDYVTLKA 488
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
349-706 |
4.56e-17 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 83.48 E-value: 4.56e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 349 YTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGST-VSIRKKFSASNYFADC 427
Cdd:cd05917 9 FTSGTTGSPKGATLTHHNIVNNGYFIGERLGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATmVFPSPSFDPLAVLEAI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 428 AKYNATI-----GQYIGEMARyilaTKPSEYDQKH-RVRLVFGNGLRPQIWPQFVQRFNIAKVGEFYGATEGNANIMNHD 501
Cdd:cd05917 89 EKEKCTAlhgvpTMFIAELEH----PDFDKFDLSSlRTGIMAGAPCPPELMKRVIEVMNMKDVTIAYGMTETSPVSTQTR 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 502 NTVGA---IGFVSRILPKIYpISIIraDPDTGEpirdrnglcqLCAPNEPGVFigkIVKGnpSREFLGYV-DEKASAKKI 577
Cdd:cd05917 165 TDDSIekrVNTVGRIMPHTE-AKIV--DPEGGI----------VPPVGVPGEL---CIRG--YSVMKGYWnDPEKTAEAI 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 578 VKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPH-TEGRAGMAA 656
Cdd:cd05917 227 DGDGWLH------TGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGV--PDeRYGEEVCAW 298
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 939619614 657 I-YDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQ 706
Cdd:cd05917 299 IrLKEGAELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
177-296 |
4.94e-17 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 84.81 E-value: 4.94e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVItPlI 256
Cdd:COG1021 26 TLGDLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAI-P-V 103
|
90 100 110 120
....*....|....*....|....*....|....*....|....*..
gi 939619614 257 NT--NLRGPSLLHSITVAHCSALIY-----GEDFLEAVTDVAKDLPA 296
Cdd:COG1021 104 FAlpAHRRAEISHFAEQSEAVAYIIpdrhrGFDYRALARELQAEVPS 150
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
345-700 |
7.74e-17 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 82.07 E-value: 7.74e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 345 LVYI-YTSGTTGLPKAAVISHSRYL-FIAAGIHyTMGFQEEDIFYTPLPLYHTAGGIMCMgqSVLF-GSTVSIRKKFSAS 421
Cdd:cd17633 2 PFYIgFTSGTTGLPKAYYRSERSWIeSFVCNED-LFNISGEDAILAPGPLSHSLFLYGAI--SALYlGGTFIGQRKFNPK 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 422 NYFADCAKYNATIGQYIGEMaryiLATKPSEYDQKHRVRLVF--GNGLRPQIWPQFVQRFNIAKVGEFYGATEGNANIMN 499
Cdd:cd17633 79 SWIRKINQYNATVIYLVPTM----LQALARTLEPESKIKSIFssGQKLFESTKKKLKNIFPKANLIEFYGTSELSFITYN 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 500 HDNTVGAIGFVSRILPKIypisiiradpdtgePIRDRnglcqlcapNEPGVFIGKI-VKGNpsREFLGYVDEKASAKkiv 578
Cdd:cd17633 155 FNQESRPPNSVGRPFPNV--------------EIEIR---------NADGGEIGKIfVKSE--MVFSGYVRGGFSNP--- 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 579 KDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGvtIPHTEGRAGMAAIY 658
Cdd:cd17633 207 DGWMSVGDIGYV-------DEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVG--IPDARFGEIAVALY 277
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 939619614 659 DPERELDLDVFAAsLAKVLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd17633 278 SGDKLTYKQLKRF-LKQKLSRYEIPKKIIFVDSLPYTSSGKI 318
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
187-400 |
8.64e-17 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 83.77 E-value: 8.64e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 187 QAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINtnlrgPSLL 266
Cdd:PRK09029 14 QVRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLN-----PQLP 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 267 HSITVAHCSALiyGEDFleAVTDVAKDLPANLTLfqfnnennnseteknipqaknLNALLTTASYEKPnktqvNHHDKLV 346
Cdd:PRK09029 89 QPLLEELLPSL--TLDF--ALVLEGENTFSALTS---------------------LHLQLVEGAHAVA-----WQPQRLA 138
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 347 -YIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAG-GIM 400
Cdd:PRK09029 139 tMTLTSGSTGLPKAAVHTAQAHLASAEGVLSLMPFTAQDSWLLSLPLFHVSGqGIV 194
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
177-706 |
1.09e-16 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 83.77 E-value: 1.09e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLGLSKIGVITPL 255
Cdd:PRK08751 26 TVAEVFATSVAKFADRPAYHSFGKTITYREADQLVEQFAAYLLGElQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVN 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 INTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLP---------------ANLTLFQFNNENNNSET-EKNIPQA 319
Cdd:PRK08751 106 VNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIADTPvkqvittglgdmlgfPKAALVNFVVKYVKKLVpEYRINGA 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 320 KNLNALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHY---TMGFQEE--DIFYTPLPLYH 394
Cdd:PRK08751 186 IRFREALALGRKHSMPTLQIEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQwlaGTGKLEEgcEVVITALPLYH 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 395 ----TAGGIMCMGqsvLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQ 470
Cdd:PRK08751 266 ifalTANGLVFMK---IGGCNHLISNPRDMPGFVKELKKTRFTAFTGVNTLFNGLLNTPGFDQIDFSSLKMTLGGGMAVQ 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 471 --IWPQFVQRFNIAKVgEFYGATEGNA----NIMNHDNTVGAIGFvsrilpkiyPIsiiradPDTGEPIRDRNGlcQLCA 544
Cdd:PRK08751 343 rsVAERWKQVTGLTLV-EAYGLTETSPaaciNPLTLKEYNGSIGL---------PI------PSTDACIKDDAG--TVLA 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 545 PNEPGVFIGK---IVKGNPSREflgyvDEkaSAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVS 621
Cdd:PRK08751 405 IGEIGELCIKgpqVMKGYWKRP-----EE--TAKVMDADGWLH------TGDIARMDEQGFVYIVDRKKDMILVSGFNVY 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 622 TSEVEAQVSNVAGYKDTVVYGVTiPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLR 701
Cdd:PRK08751 472 PNEIEDVIAMMPGVLEVAAVGVP-DEKSGEIVKVVIVKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKIL 550
|
....*
gi 939619614 702 KVDLQ 706
Cdd:PRK08751 551 RRELR 555
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
190-700 |
1.42e-16 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 83.13 E-value: 1.42e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLintNLRGPsllhs 268
Cdd:cd17643 1 PEAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGgAYVPI---DPAYP----- 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 269 itvAHCSALIygedfleaVTDVAkdlpanltlfqfnnennnseteknipqaknLNALLTTAsyekpnktqvnhhDKLVY- 347
Cdd:cd17643 73 ---VERIAFI--------LADSG------------------------------PSLLLTDP-------------DDLAYv 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISHS--RYLFIAAGihYTMGFQEEDIFYtplpLYHTaggiMCMGQSV-------LFGSTVSIRKKF 418
Cdd:cd17643 99 IYTSGSTGRPKGVVVSHAnvLALFAATQ--RWFGFNEDDVWT----LFHS----YAFDFSVweiwgalLHGGRLVVVPYE 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 419 ---SASNYFADCAKYNATI-GQYIGEMARYILATKpSEYDQKHRVRLVF--GNGLRPQIWPQFVQRFNIAK--VGEFYGA 490
Cdd:cd17643 169 varSPEDFARLLRDEGVTVlNQTPSAFYQLVEAAD-RDGRDPLALRYVIfgGEALEAAMLRPWAGRFGLDRpqLVNMYGI 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 491 TEgnanimnhdNTVgaigFVS--RILPKIYP---ISII-RADPDTGEPIRDRNGlcqlcAPNEPGVfIGKIVKGNP--SR 562
Cdd:cd17643 248 TE---------TTV----HVTfrPLDAADLPaaaASPIgRPLPGLRVYVLDADG-----RPVPPGV-VGELYVSGAgvAR 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 563 EFLGYVDEkaSAKKIVKDVFKH-GDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVy 641
Cdd:cd17643 309 GYLGRPEL--TAERFVANPFGGpGSRMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAV- 385
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 939619614 642 gvtIPHT--EGRAGMAAIYDPERELDLDVFA--ASLAKVLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd17643 386 ---IVREdePGDTRLVAYVVADDGAAADIAElrALLKELLPDYMVPARYVPLDALPLTVNGKL 445
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
184-412 |
1.49e-16 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 83.44 E-value: 1.49e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 184 RNVQAHPDKVAVV------SETQRWTFRQVNEHANKVANVLQAQGyKKGDVVALLLENRAEYVATWLGLSKIGVI-TPLI 256
Cdd:cd05931 1 RRAAARPDRPAYTflddegGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIaVPLP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRG--PSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQFnnennnseteknipqAKNLNALLTTASYEKP 334
Cdd:cd05931 80 PPTPGRhaERLAAILADAGPRVVLTTAAALAAVRAFAASRPAAGTPRLL---------------VVDLLPDTSAADWPPP 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 NKTqvnhHDKLVYI-YTSGTTGLPKAAVISHsRYLFI-AAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTV 412
Cdd:cd05931 145 SPD----PDDIAYLqYTSGSTGTPKGVVVTH-RNLLAnVRQIRRAYGLDPGDVVVSWLPLYHDMGLIGGLLTPLYSGGPS 219
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
180-647 |
1.66e-16 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 83.18 E-value: 1.66e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLGLSKIGVItpLINT 258
Cdd:PRK08974 27 DMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNGlGLKKGDRVALMMPNLLQYPIALFGILRAGMI--VVNV 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 259 N-LRGP-SLLHSITVAHCSALIYGEDF---LEAV-----------TDVAKDLP-ANLTLFQFNNENNNSET-EKNIPQAK 320
Cdd:PRK08974 105 NpLYTPrELEHQLNDSGAKAIVIVSNFahtLEKVvfktpvkhvilTRMGDQLStAKGTLVNFVVKYIKRLVpKYHLPDAI 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 321 NLNALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRY---LFIAAGIHYTMGFQEEDIFYTPLPLYHT-A 396
Cdd:PRK08974 185 SFRSALHKGRRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMlanLEQAKAAYGPLLHPGKELVVTALPLYHIfA 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 397 GGIMCMgqsvLF----GSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKP-SEYDQKhRVRLVFGNGLRPQi 471
Cdd:PRK08974 265 LTVNCL----LFielgGQNLLITNPRDIPGFVKELKKYPFTAITGVNTLFNALLNNEEfQELDFS-SLKLSVGGGMAVQ- 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 472 wpQFV----QRFNIAKVGEFYGATEGN----ANIMNHDNTVGAIGFvsrilpkiyPIsiiradPDTGEPIRDRNGlcQLC 543
Cdd:PRK08974 339 --QAVaerwVKLTGQYLLEGYGLTECSplvsVNPYDLDYYSGSIGL---------PV------PSTEIKLVDDDG--NEV 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 544 APNEPGVFigkIVKGnpSREFLGYVDEKASAKKIVKDvfkhGDMAfiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTS 623
Cdd:PRK08974 400 PPGEPGEL---WVKG--PQVMLGYWQRPEATDEVIKD----GWLA--TGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPN 468
|
490 500
....*....|....*....|....
gi 939619614 624 EVEAQVSnvAGYKDTVVYGVTIPH 647
Cdd:PRK08974 469 EIEDVVM--LHPKVLEVAAVGVPS 490
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
182-647 |
2.26e-16 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 83.14 E-value: 2.26e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 182 FERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLR 261
Cdd:PLN03102 20 LKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLD 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 262 GPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSETEKNIPQAKNLN--ALL-----TTASYEKP 334
Cdd:PLN03102 100 ATSIAAILRHAKPKILFVDRSFEPLAREVLHLLSSEDSNLNLPVIFIHEIDFPKRPSSEELDyeCLIqrgepTPSLVARM 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 NKTQvNHHDKLVYIYTSGTTGLPKAAVISH-SRYL-FIAAGIHYTMGFQEedIFYTPLPLYHTAGGIMCMGQSVLFGSTV 412
Cdd:PLN03102 180 FRIQ-DEHDPISLNYTSGTTADPKGVVISHrGAYLsTLSAIIGWEMGTCP--VYLWTLPMFHCNGWTFTWGTAARGGTSV 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 413 SIRkKFSASNYFADCAKYNATIGQYIGEMARYILatKPSEYDQKHR---VRLVFGNGLRPQIWPQFVQRFNIaKVGEFYG 489
Cdd:PLN03102 257 CMR-HVTAPEIYKNIEMHNVTHMCCVPTVFNILL--KGNSLDLSPRsgpVHVLTGGSPPPAALVKKVQRLGF-QVMHAYG 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 490 ATEGNANIMN---HDNTVGAIGFVSRILPKIYPISIIR-ADPDTgepirdRNGLCQLCAPNEpGVFIGKIV-KGnpSREF 564
Cdd:PLN03102 333 LTEATGPVLFcewQDEWNRLPENQQMELKARQGVSILGlADVDV------KNKETQESVPRD-GKTMGEIViKG--SSIM 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 565 LGYVDEkasaKKIVKDVFKHGDMAfiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVygVT 644
Cdd:PLN03102 404 KGYLKN----PKATSEAFKHGWLN--TGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAV--VA 475
|
...
gi 939619614 645 IPH 647
Cdd:PLN03102 476 MPH 478
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
172-718 |
3.66e-16 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 83.29 E-value: 3.66e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 172 ERLNYTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG- 250
Cdd:PRK12467 508 EYAPDCVHQLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGg 587
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 251 VITPLINTNLRgPSLLHSITVAHCsALIYGEDFLEAVTDVAKDLPAnLTLFQfnnennnseteknipqaknLNALLTTAS 330
Cdd:PRK12467 588 AYVPLDPEYPQ-DRLAYMLDDSGV-RLLLTQSHLLAQLPVPAGLRS-LCLDE-------------------PADLLCGYS 645
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 331 YEKPNKTQvnHHDKLVY-IYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGqSVLFG 409
Cdd:PRK12467 646 GHNPEVAL--DPDNLAYvIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTELFG-ALASG 722
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 410 STVSIRKK---FSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIAKVGE 486
Cdd:PRK12467 723 ATLHLLPPdcaRDAEAFAALMADQGVTVLKIVPSHLQALLQASRVALPRPQRALVCGGEALQVDLLARVRALGPGARLIN 802
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 487 FYGATEgnanimnhdNTVGAIGFVSRILPKIYPISII-RADPDTGEPIRDRNglCQLCAPNEPG-VFIGK--IVKGNPSR 562
Cdd:PRK12467 803 HYGPTE---------TTVGVSTYELSDEERDFGNVPIgQPLANLGLYILDHY--LNPVPVGVVGeLYIGGagLARGYHRR 871
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 563 EFLgyvdekaSAKKIVKDVF-KHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVy 641
Cdd:PRK12467 872 PAL-------TAERFVPDPFgADGGRLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVV- 943
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 642 gVTIPHTEGRAGMAAIY------DPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQKegYDPNAI 715
Cdd:PRK12467 944 -LAQPGDAGLQLVAYLVpaavadGAEHQATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPK--PDASAV 1020
|
...
gi 939619614 716 KDA 718
Cdd:PRK12467 1021 QAT 1023
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
190-705 |
8.95e-16 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 80.60 E-value: 8.95e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKI-GVITPL--------INTNL 260
Cdd:cd17656 2 PDAVAVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAgGAFVPIdpeypeerRIYIM 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 261 RGPSLLHSITVAHCSALIY--GEDFLEAVTDVAKDLPANLTLfqfnnennnseteknipqaknlnallttasyekpnktq 338
Cdd:cd17656 82 LDSGVRVVLTQRHLKSKLSfnKSTILLEDPSISQEDTSNIDY-------------------------------------- 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 339 VNHHDKLVY-IYTSGTTGLPKAAVISH-------------SRYLFIAAGIHY-TMGFQ--EEDIFYTPLplyhtAGGIMC 401
Cdd:cd17656 124 INNSDDLLYiIYTSGTTGKPKGVQLEHknmvnllhferekTNINFSDKVLQFaTCSFDvcYQEIFSTLL-----SGGTLY 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 402 MGQ-------------------SVLFGSTVSIRKKFS---ASNYFADCAKYNATIGQYIgemaryILATKPSEYDQKHRV 459
Cdd:cd17656 199 IIReetkrdveqlfdlvkrhniEVVFLPVAFLKFIFSereFINRFPTCVKHIITAGEQL------VITNEFKEMLHEHNV 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 460 RLvfgnglrpqiwpqfvqrFNiakvgeFYGATEGNANIMnhdntvgaigfvSRILPKIYpisiIRADPDTGEPIR----- 534
Cdd:cd17656 273 HL-----------------HN------HYGPSETHVVTT------------YTINPEAE----IPELPPIGKPISntwiy 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 535 --DRNGLCQLCapnepGVfIGK--IVKGNPSReflGYVD-EKASAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRT 609
Cdd:cd17656 314 ilDQEQQLQPQ-----GI-VGElyISGASVAR---GYLNrQELTAEKFFPDPFDPNERMYRTGDLARYLPDGNIEFLGRA 384
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 610 GDTFRWKGENVSTSEVEAQVSNVAGYKDTVVygVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLL 689
Cdd:cd17656 385 DHQVKIRGYRIELGEIEAQLLNHPGVSEAVV--LDKADDKGEKYLCAYFVMEQELNISQLREYLAKQLPEYMIPSFFVPL 462
|
570
....*....|....*.
gi 939619614 690 TKVDLTGTFKLRKVDL 705
Cdd:cd17656 463 DQLPLTPNGKVDRKAL 478
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
177-708 |
9.12e-16 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 80.83 E-value: 9.12e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVItpLI 256
Cdd:PRK07059 24 SLADLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYV--VV 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTN-LRGP-SLLHSITVAHCSALIYGEDF---LEAV---TDVAKDLPANL--------TLFQFNNENNNSET-EKNIPQA 319
Cdd:PRK07059 102 NVNpLYTPrELEHQLKDSGAEAIVVLENFattVQQVlakTAVKHVVVASMgdllgfkgHIVNFVVRRVKKMVpAWSLPGH 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 320 KNLNALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTM-------GFQEEDIFYTPLPL 392
Cdd:PRK07059 182 VRFNDALAEGARQTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVANVLQMEAWLqpafekkPRPDQLNFVCALPL 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 393 YH----TAGGIMCM---GQSVLFGSTVSIR------KKFSASNYFADCAKYNAtigqyigemaryiLATKPsEYDQK--H 457
Cdd:PRK07059 262 YHifalTVCGLLGMrtgGRNILIPNPRDIPgfikelKKYQVHIFPAVNTLYNA-------------LLNNP-DFDKLdfS 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 458 RVRLVFGNGLRpqiwpqfVQRfNIAK---------VGEFYGATE----GNANIMNHDNTVGAIGFvsrilpkiyPIsiir 524
Cdd:PRK07059 328 KLIVANGGGMA-------VQR-PVAErwlemtgcpITEGYGLSEtspvATCNPVDATEFSGTIGL---------PL---- 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 525 adPDTGEPIRDRNGlcqlcapNE-PGVFIGKIVKGNPsREFLGY---VDEkaSAKKIVKDVFkhgdmaFISGDLLVADEK 600
Cdd:PRK07059 387 --PSTEVSIRDDDG-------NDlPLGEPGEICIRGP-QVMAGYwnrPDE--TAKVMTADGF------FRTGDVGVMDER 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 601 GYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTeGRAGMAAIYDPERELDLDVFAASLAKVLPAY 680
Cdd:PRK07059 449 GYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLEVAAVGVPDEHS-GEAVKLFVVKKDPALTEEDVKAFCKERLTNY 527
|
570 580
....*....|....*....|....*...
gi 939619614 681 ARPQIIRLLTKVDLTGTFKLRKVDLQKE 708
Cdd:PRK07059 528 KRPKFVEFRTELPKTNVGKILRRELRDG 555
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
190-702 |
9.67e-16 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 80.41 E-value: 9.67e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLintnlrGPSllhs 268
Cdd:cd12116 1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGaAYVPL------DPD---- 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 269 itvahcsaliYGEDFLEAvtdVAKDLPANLTLFQFNNENNNSETEKNIPqaknLNALLTTASYEKPNKTQVNHHDKLVyI 348
Cdd:cd12116 71 ----------YPADRLRY---ILEDAEPALVLTDDALPDRLPAGLPVLL----LALAAAAAAPAAPRTPVSPDDLAYV-I 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 349 YTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEED--IFYTP-----------LPLYHTAGGIMCMGQSVLFGSTVSir 415
Cdd:cd12116 133 YTSGSTGRPKGVVVSHRNLVNFLHSMRERLGLGPGDrlLAVTTyafdisllellLPLLAGARVVIAPRETQRDPEALA-- 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 416 kkfsasnyfADCAKYNATIGQYIGEMARYILAtkpSEYDQKHRVR-LVFGNGLRPQIWPQFVQRfnIAKVGEFYGATEgn 494
Cdd:cd12116 211 ---------RLIEAHSITVMQATPATWRMLLD---AGWQGRAGLTaLCGGEALPPDLAARLLSR--VGSLWNLYGPTE-- 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 495 animnhdNTVGAIgfVSRILPKIYPISIIRADPDTGEPIRDRNGlcQLCAPNEPG-VFIGKI-VKgnpsrefLGYVDEKA 572
Cdd:cd12116 275 -------TTIWST--AARVTAAAGPIPIGRPLANTQVYVLDAAL--RPVPPGVPGeLYIGGDgVA-------QGYLGRPA 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 573 -SAKKIVKDVFKH-GDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVvygVTIPHTEG 650
Cdd:cd12116 337 lTAERFVPDPFAGpGSRLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAA---VVVREDGG 413
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 939619614 651 RAGMAAIYDPERE--LDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL-RK 702
Cdd:cd12116 414 DRRLVAYVVLKAGaaPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLdRK 468
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
182-707 |
1.01e-15 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 80.45 E-value: 1.01e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 182 FERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG----VITP--- 254
Cdd:cd17655 3 FEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGgaylPIDPdyp 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 --LINTNLR--GPSLLhsitVAHcSALIYGEDFLEAVTDVAKDlpanltlfqfnnennnseteknipqaknlnallTTAS 330
Cdd:cd17655 83 eeRIQYILEdsGADIL----LTQ-SHLQPPIAFIGLIDLLDED---------------------------------TIYH 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 331 YEKPNKTQVNHHDKLVY-IYTSGTTGLPKAAVISHSR--YLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMcmgQSVL 407
Cdd:cd17655 125 EESENLEPVSKSDDLAYvIYTSGSTGKPKGVMIEHRGvvNLVEWANKVIYQGEHLRVALFASISFDASVTEIF---ASLL 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 408 FGSTVSIRKKFSASNYFADCA---KYNATIGQ----YIGEMARyilATKPSEYDQKHrvRLVFGNGLRPQIWPQFVQRFN 480
Cdd:cd17655 202 SGNTLYIVRKETVLDGQALTQyirQNRITIIDltpaHLKLLDA---ADDSEGLSLKH--LIVGGEALSTELAKKIIELFG 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 481 IA-KVGEFYGATEgnanimnhdNTVGAIGFVsrilpkIYPISIIRADPDTGEP-------IRDRNGlcQLCAPNEPG-VF 551
Cdd:cd17655 277 TNpTITNAYGPTE---------TTVDASIYQ------YEPETDQQVSVPIGKPlgntriyILDQYG--RPQPVGVAGeLY 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 552 IG--KIVKGNPSREFLgyvdekaSAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQV 629
Cdd:cd17655 340 IGgeGVARGYLNRPEL-------TAEKFVDDPFVPGERMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARL 412
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 939619614 630 SNVAGYKDTVVygVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTfklRKVDLQK 707
Cdd:cd17655 413 LQHPDIKEAVV--IARKDEQGQNYLCAYIVSEKELPVAQLREFLARELPDYMIPSYFIKLDEIPLTPN---GKVDRKA 485
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
180-700 |
1.30e-15 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 80.05 E-value: 1.30e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLint 258
Cdd:cd12115 3 DLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGaAYVPL--- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 259 nlrGPSllhsitvahcsaliYGEDFLEAVTDVAKDLpanltlfqfnnennnseteknipqaknlnALLTTAsyekpnktq 338
Cdd:cd12115 80 ---DPA--------------YPPERLRFILEDAQAR-----------------------------LVLTDP--------- 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 339 vnhhDKLVY-IYTSGTTGLPKAAVISH-SRYLFIA-AGIHYTmgfQEE--------------DIFYTPLPLyhTAGGIMC 401
Cdd:cd12115 105 ----DDLAYvIYTSGSTGRPKGVAIEHrNAAAFLQwAAAAFS---AEElagvlastsicfdlSVFELFGPL--ATGGKVV 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 402 MGQSVLFGSTVSIRKKFSASNyfadcakynatigqyigemaryilaTKPSEYDQ--KHR-----VRLV--FGNGLRPQIW 472
Cdd:cd12115 176 LADNVLALPDLPAAAEVTLIN-------------------------TVPSAAAEllRHDalpasVRVVnlAGEPLPRDLV 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 473 PQFVQRFNIAKVGEFYGATEgnanimnhDNTVGAIGFVSRILPKiyPISIIRADPDTGEPIRDRNGlcqlcAPNEPGVfI 552
Cdd:cd12115 231 QRLYARLQVERVVNLYGPSE--------DTTYSTVAPVPPGASG--EVSIGRPLANTQAYVLDRAL-----QPVPLGV-P 294
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 553 GKIVKGNP--SREFLGyvDEKASAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVS 630
Cdd:cd12115 295 GELYIGGAgvARGYLG--RPGLTAERFLPDPFGPGARLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALR 372
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 631 NVAGYKDTVVYGVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd12115 373 SIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKI 442
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
202-627 |
1.37e-15 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 79.85 E-value: 1.37e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 202 WTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAE-YVATwLGLSKIG-VITPLINTnlrgpsllhsitvahcsaliY 279
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPElYFSM-LGIGKIGaVICPLFSA--------------------F 59
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 280 GEdflEAVTDVAKDLPANLtlfqfnnennnseteknipqaknlnaLLTTAS-YEKPNKtqvnhHDKLVYIYTSGTTGLPK 358
Cdd:cd05969 60 GP---EAIRDRLENSEAKV--------------------------LITTEElYERTDP-----EDPTLLHYTSGTTGTPK 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 359 AAVISHSRYLFIAAGIHYTMGFQEEDIFY-TPLPLYHTaGGIMCMGQSVLFGST-VSIRKKFSASNYFADCAKYNATIgQ 436
Cdd:cd05969 106 GVLHVHDAMIFYYFTGKYVLDLHPDDIYWcTADPGWVT-GTVYGIWAPWLNGVTnVVYEGRFDAESWYGIIERVKVTV-W 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 437 YIGEMARYILATKPSE----YDQKH-RVRLVFGNGLRPQIWPQFVQRFNIaKVGEFYGATE-GNANIMNHDNTVGAIGFV 510
Cdd:cd05969 184 YTAPTAIRMLMKEGDElarkYDLSSlRFIHSVGEPLNPEAIRWGMEVFGV-PIHDTWWQTEtGSIMIANYPCMPIKPGSM 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 511 SRILPKIyPISIIRADpdtGEPIRdrnglcqlcaPNEPGVFIGKivKGNPSReFLGYVDEKASAKKIVKDVFkhgdmaFI 590
Cdd:cd05969 263 GKPLPGV-KAAVVDEN---GNELP----------PGTKGILALK--PGWPSM-FRGIWNDEERYKNSFIDGW------YL 319
|
410 420 430
....*....|....*....|....*....|....*..
gi 939619614 591 SGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEA 627
Cdd:cd05969 320 TGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVES 356
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
343-643 |
1.48e-15 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 78.47 E-value: 1.48e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 343 DKLVYIYTSGTTGLPKAAVISHSRylFIAAGIH--YTMGFQEEDIFYTPLPLYHTAGgiMCMGQSVLF--GSTVsIRKKF 418
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGN--LIAANLQliHAMGLTEADVYLNMLPLFHIAG--LNLALATFHagGANV-VMEKF 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 419 SASNYFADCAKYNATigqYIGEMARyILATKPSEYDQKHR----VRLVFGNGLrpqiwPQFVQRFNIAKVGEF---YGAT 491
Cdd:cd17637 76 DPAEALELIEEEKVT---LMGSFPP-ILSNLLDAAEKSGVdlssLRHVLGLDA-----PETIQRFEETTGATFwslYGQT 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 492 E--GNANIMNHDNTVGAIGfvsrilpKIYPISIIRADPDTGEPIRdrnglcqlcapnePGVfIGKIVKGNPSrEFLGYVD 569
Cdd:cd17637 147 EtsGLVTLSPYRERPGSAG-------RPGPLVRVRIVDDNDRPVP-------------AGE-TGEIVVRGPL-VFQGYWN 204
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 570 EKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWK--GENVSTSEVEAQVSNVAGYKDTVVYGV 643
Cdd:cd17637 205 LPELTAYTFRNGWHH------TGDLGRFDEDGYLWYAGRKPEKELIKpgGENVYPAEVEKVILEHPAIAEVCVIGV 274
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
190-708 |
1.61e-15 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 80.09 E-value: 1.61e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSI 269
Cdd:PRK06178 47 PQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYEL 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 270 TVAHCSALIYGEDFLE--------------AVTDVAKDLPANLTLfqfNNENNNSETEKNIPQAKNLNALLTTASyeKPN 335
Cdd:PRK06178 127 NDAGAEVLLALDQLAPvveqvraetslrhvIVTSLADVLPAEPTL---PLPDSLRAPRLAAAGAIDLLPALRACT--APV 201
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 336 KTQVNHHDKLVYI-YTSGTTGLPKAAVISHSRYLFIAAGIH-YTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVS 413
Cdd:PRK06178 202 PLPPPALDALAALnYTGGTTGMPKGCEHTQRDMVYTAAAAYaVAVVGGEDSVFLSFLPEFWIAGENFGLLFPLFSGATLV 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 414 IRKKFSASNYFADCAKYNATIGQYIGEMARYILAT-KPSEYDQK--HRVRLV-FGNGLRP---QIWPQFVQrfniAKVGE 486
Cdd:PRK06178 282 LLARWDAVAFMAAVERYRVTRTVMLVDNAVELMDHpRFAEYDLSslRQVRVVsFVKKLNPdyrQRWRALTG----SVLAE 357
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 487 F-YGATEGN------ANIMNHD-NTVGAIGFVSRILP----KIypisiirADPDTGEPIrdrnglcQLCAPnepgvfiGK 554
Cdd:PRK06178 358 AaWGMTETHtcdtftAGFQDDDfDLLSQPVFVGLPVPgtefKI-------CDFETGELL-------PLGAE-------GE 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 555 IVKGNPSReFLGYVDEKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAG 634
Cdd:PRK06178 417 IVVRTPSL-LKGYWNKPEATAEALRDGWLH------TGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPA 489
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 939619614 635 YKDTVVYGVTIPHtEGRAGMAAI-YDPERELDLDVFAASLAKVLPAYARPQiIRLLTKVDLTGTFKLRKVDLQKE 708
Cdd:PRK06178 490 VLGSAVVGRPDPD-KGQVPVAFVqLKPGADLTAAALQAWCRENMAVYKVPE-IRIVDALPMTATGKVRKQDLQAL 562
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
173-627 |
1.93e-15 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 80.23 E-value: 1.93e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 173 RLNyTVADVFERNVQAHPDKVAVVSETQR-----WTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLS 247
Cdd:cd05968 59 RMN-IVEQLLDKWLADTRTRPALRWEGEDgtsrtLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVA 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 248 KIG-VITPLINTNLRGPSLLHsITVAHCSALIYGEDFLEavtdvaKDLPANLTlfqfnneNNNSETEKNIPQAKNL---- 322
Cdd:cd05968 138 RIGgIVVPIFSGFGKEAAATR-LQDAEAKALITADGFTR------RGREVNLK-------EEADKACAQCPTVEKVvvvr 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 323 ---NALLTT----ASYEKPNKTQVNH------HDKLVYIYTSGTTGLPKAAVISHSRY-LFIAAGIHYTMGFQEEDIFYt 388
Cdd:cd05968 204 hlgNDFTPAkgrdLSYDEEKETAGDGaertesEDPLMIIYTSGTTGKPKGTVHVHAGFpLKAAQDMYFQFDLKPGDLLT- 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 389 plplYHTAGGIMcMGQSVLFGSTVSIRKKFSASNY--FADCAKYNATIGQY----IGEMARYILATKP--SEYDQKH--- 457
Cdd:cd05968 283 ----WFTDLGWM-MGPWLIFGGLILGATMVLYDGApdHPKADRLWRMVEDHeithLGLSPTLIRALKPrgDAPVNAHdls 357
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 458 RVRLVFGNG--LRPQIWPQFVQRFNIAKVG--EFYGATEGNANImnhdntvgaigFVSRILPKIYPISIIRADP------ 527
Cdd:cd05968 358 SLRVLGSTGepWNPEPWNWLFETVGKGRNPiiNYSGGTEISGGI-----------LGNVLIKPIKPSSFNGPVPgmkadv 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 528 --DTGEPIRDRNGLCQLCAPnEPGVFIGkiVKGNPSREFLGYVDEkasakkiVKDVFKHGDMAFIsgdllvaDEKGYLYF 605
Cdd:cd05968 427 ldESGKPARPEVGELVLLAP-WPGMTRG--FWRDEDRYLETYWSR-------FDNVWVHGDFAYY-------DEEGYFYI 489
|
490 500
....*....|....*....|..
gi 939619614 606 KDRTGDTFRWKGENVSTSEVEA 627
Cdd:cd05968 490 LGRSDDTINVAGKRVGPAEIES 511
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
343-702 |
2.31e-15 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 78.07 E-value: 2.31e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 343 DKLVYIYTSGTTGLPKAAVISHSryLFIAAGIH---YTMGFQEEDIFYTPLPLYHTaGGIMCMGQSVLFGSTVSIRKKF- 418
Cdd:cd17635 2 DPLAVIFTSGTTGEPKAVLLANK--TFFAVPDIlqkEGLNWVVGDVTYLPLPATHI-GGLWWILTCLIHGGLCVTGGENt 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 419 SASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRP-QIWPQFVQRFNIAKVGEFYGATE-GNAN 496
Cdd:cd17635 79 TYKSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATVPSLRLIGYGGSRAiAADVRFIEATGLTNTAQVYGLSEtGTAL 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 497 IMNHDNTVGAIGFVSRILPKIypiSIIRADPDTGEPIRDRNGLCQLCAPnepgvfigkivkgnpsREFLGYVDE-KASAK 575
Cdd:cd17635 159 CLPTDDDSIEINAVGRPYPGV---DVYLAATDGIAGPSASFGTIWIKSP----------------ANMLGYWNNpERTAE 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 576 KIVKDVFKhgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYgvTIPHTEGRAGMA 655
Cdd:cd17635 220 VLIDGWVN-------TGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACY--EISDEEFGELVG 290
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 939619614 656 AIYDPERELDLDVFAASLAKV---LPAYARPQIIRLLTKVDLTGTFKLRK 702
Cdd:cd17635 291 LAVVASAELDENAIRALKHTIrreLEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
160-702 |
3.56e-15 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 78.88 E-value: 3.56e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 160 AYIKLLRYTKRHERLNYTVADVferNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEY 239
Cdd:PRK13383 22 AVLRLLREASRGGTNPYTLLAV---TAARWPGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGF 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 240 VATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDL----PAnltlfqfnnennnsetekn 315
Cdd:PRK13383 99 VTAVFAVGLLGADVVPISTEFRSDALAAALRAHHISTVVADNEFAERIAGADDAVavidPA------------------- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 316 ipqaknlnallTTASYEKPNKTQVNHHDKLVYIyTSGTTGLPKAAvishSRYLFIAAGIHYTMGFQEEDIFYT------P 389
Cdd:PRK13383 160 -----------TAGAEESGGRPAVAAPGRIVLL-TSGTTGKPKGV----PRAPQLRSAVGVWVTILDRTRLRTgsrisvA 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 390 LPLYHTAGGIMCMgQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPS--EYDQKHRVRLVFGNGL 467
Cdd:PRK13383 224 MPMFHGLGLGMLM-LTIALGGTVLTHRHFDAEAALAQASLHRADAFTAVPVVLARILELPPRvrARNPLPQLRVVMSSGD 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 468 RpqIWPQFVQRFnIAKVGEF----YGATEGNanimnhdntVGAIGFVSRIlpKIYPISIIRadPDTGEPIR--DRNGlcQ 541
Cdd:PRK13383 303 R--LDPTLGQRF-MDTYGDIlyngYGSTEVG---------IGALATPADL--RDAPETVGK--PVAGCPVRilDRNN--R 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 542 LCAPNEPG-VFIGKIVKGNpsreflGYVDekASAKKIVKDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENV 620
Cdd:PRK13383 365 PVGPRVTGrIFVGGELAGT------RYTD--GGGKAVVDGMTSTGDMGYL-------DNAGRLFIVGREDDMIISGGENV 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 621 STSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFK- 699
Cdd:PRK13383 430 YPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSGVDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKv 509
|
...
gi 939619614 700 LRK 702
Cdd:PRK13383 510 LRK 512
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
180-702 |
4.91e-15 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 78.35 E-value: 4.91e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLint 258
Cdd:cd05918 3 DLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGgAFVPL--- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 259 nlrGPSL----LHSItvahcsaliygedfleavtdvAKDLPANLTLfqfnnennnseteknipqaknlnallttasyekp 334
Cdd:cd05918 80 ---DPSHplqrLQEI---------------------LQDTGAKVVL---------------------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 nktqVNHHDKLVY-IYTSGTTGLPKAAVISHSrylFIAAGIHYT---MGFQEE----------------DIFYTplplyh 394
Cdd:cd05918 102 ----TSSPSDAAYvIFTSGSTGKPKGVVIEHR---ALSTSALAHgraLGLTSEsrvlqfasytfdvsilEIFTT------ 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 395 taggimcmgqsVLFGSTVSIRKKFSASNYFADCA-KYNATIGQYIGEMARYI-LATKPSeydqkhrVR-LVFGnG--LRP 469
Cdd:cd05918 169 -----------LAAGGCLCIPSEEDRLNDLAGFInRLRVTWAFLTPSVARLLdPEDVPS-------LRtLVLG-GeaLTQ 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 470 ---QIWPQFVQRFNiakvgeFYGATEgnANIMNHDNTVGAIGFVSRI-LPkiYPISIIRADPDTGEpirdrnglcQLCAP 545
Cdd:cd05918 230 sdvDTWADRVRLIN------AYGPAE--CTIAATVSPVVPSTDPRNIgRP--LGATCWVVDPDNHD---------RLVPI 290
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 546 NEPG--VFIGKIVkgnpSREFLGyvDEKASAKKIVKD-------VFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWK 616
Cdd:cd05918 291 GAVGelLIEGPIL----ARGYLN--DPEKTAAAFIEDpawlkqeGSGRGRRLYRTGDLVRYNPDGSLEYVGRKDTQVKIR 364
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 617 GENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGM--AAIYDPERELD----LDVFA--------------ASLAKV 676
Cdd:cd05918 365 GQRVELGEIEHHLRQSLPGAKEVVVEVVKPKDGSSSPQlvAFVVLDGSSSGsgdgDSLFLepsdefralvaelrSKLRQR 444
|
570 580
....*....|....*....|....*..
gi 939619614 677 LPAYARPQIIRLLTKVDLTGTFKL-RK 702
Cdd:cd05918 445 LPSYMVPSVFLPLSHLPLTASGKIdRR 471
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
183-626 |
1.37e-14 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 77.29 E-value: 1.37e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 183 ERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRG 262
Cdd:PRK08162 25 ERAAEVYPDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDA 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 263 PSLLhsITVAHCSA--LIYGEDFLEAVTDVAKDLPaNLTLFQFNNENNNSETEKNIpQAKNLNALLTTASYEKPNKTQVN 340
Cdd:PRK08162 105 ASIA--FMLRHGEAkvLIVDTEFAEVAREALALLP-GPKPLVIDVDDPEYPGGRFI-GALDYEAFLASGDPDFAWTLPAD 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 341 HHDKLVYIYTSGTTGLPKaAVISHSR--YLFIAAGI-HYTMGfQEEDIFYTpLPLYHTAGgiMCMGQSV--LFGSTVSIR 415
Cdd:PRK08162 181 EWDAIALNYTSGTTGNPK-GVVYHHRgaYLNALSNIlAWGMP-KHPVYLWT-LPMFHCNG--WCFPWTVaaRAGTNVCLR 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 416 kKFSASNYFADCAKYNATigQYIGEMARY-ILATKPSEYDQK--HRVR-LVFGNGLRPQIWPQFVQR-FNIAKVgefYGA 490
Cdd:PRK08162 256 -KVDPKLIFDLIREHGVT--HYCGAPIVLsALINAPAEWRAGidHPVHaMVAGAAPPAAVIAKMEEIgFDLTHV---YGL 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 491 TE--GNANIMNHDNTVGAIGFVSRILPKI-----YPI--SIIRADPDTGEPI-RDrnglcqlcapnepGVFIGKIV-KGN 559
Cdd:PRK08162 330 TEtyGPATVCAWQPEWDALPLDERAQLKArqgvrYPLqeGVTVLDPDTMQPVpAD-------------GETIGEIMfRGN 396
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 939619614 560 PSREflGYV-DEKASAKkivkdVFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVE 626
Cdd:PRK08162 397 IVMK--GYLkNPKATEE-----AFAGG--WFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVE 455
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
348-707 |
2.17e-14 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 75.06 E-value: 2.17e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTaGGIMCMGQSVLFGSTVSIRKKFSASnyFADC 427
Cdd:cd17630 6 ILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPLYHV-GGLAILVRSLLAGAELVLLERNQAL--AEDL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 428 AKYNATIGQYIGEMARYILaTKPSEYDQKHRVRLVFGNGlrPQIWPQFVQRFNIAKVGEF--YGATEgnanimnhdntvg 505
Cdd:cd17630 83 APPGVTHVSLVPTQLQRLL-DSGQGPAALKSLRAVLLGG--APIPPELLERAADRGIPLYttYGMTE------------- 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 506 aigFVSRILPKIYPISiirADPDTGEPIRDRnglcQLCAPNEPGVFIGkivkgnPSREFLGYVDekasaKKIVKDVFKHG 585
Cdd:cd17630 147 ---TASQVATKRPDGF---GRGGVGVLLPGR----ELRIVEDGEIWVG------GASLAMGYLR-----GQLVPEFNEDG 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 586 dmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVtiPHTEGRAGMAAIYDPERELD 665
Cdd:cd17630 206 --WFTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGV--PDEELGQRPVAVIVGRGPAD 281
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 939619614 666 LDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQK 707
Cdd:cd17630 282 PAELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRA 323
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
178-707 |
4.90e-14 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 76.53 E-value: 4.90e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 178 VADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLIN 257
Cdd:PRK12316 2005 VHQRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLD 2084
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 258 TNLRGPSLLHSITVAhcsaliyGEDFLEAVTDVAKDLPanltlfqfnnennnseteknIPQAknLNALLTTASYEKPNKT 337
Cdd:PRK12316 2085 PNYPAERLAYMLEDS-------GAALLLTQRHLLERLP--------------------LPAG--VARLPLDRDAEWADYP 2135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 338 QVN-----HHDKLVY-IYTSGTTGLPKAAVISHSRYL--FIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMcmgQSVLFG 409
Cdd:PRK12316 2136 DTApavqlAGENLAYvIYTSGSTGLPKGVAVSHGALVahCQAAGERYELSPADCELQFMSFSFDGAHEQWF---HPLLNG 2212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 410 STVSIR--KKFSASNYFADCAKYNATIG--------QYIGEMAR---------YIL---ATKPSEYDQKHRvrlvfgnGL 467
Cdd:PRK12316 2213 ARVLIRddELWDPEQLYDEMERHGVTILdfppvylqQLAEHAERdgrppavrvYCFggeAVPAASLRLAWE-------AL 2285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 468 RPQIWpqfvqrFNIakvgefYGATEGNANIM----NHDNTVGAIGFvsrilpkiyPIsiiradpdtGEPIRDRNGLC--- 540
Cdd:PRK12316 2286 RPVYL------FNG------YGPTEAVVTPLlwkcRPQDPCGAAYV---------PI---------GRALGNRRAYIlda 2335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 541 --QLCAPNEPG-VFIGKivkgnpsrEFL--GYVDEKA-SAKKIVKDVFKH-GDMAFISGDLLVADEKGYLYFKDRTGDTF 613
Cdd:PRK12316 2336 dlNLLAPGMAGeLYLGG--------EGLarGYLNRPGlTAERFVPDPFSAsGERLYRTGDLARYRADGVVEYLGRIDHQV 2407
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 614 RWKGENVSTSEVEAQVSNVAGYKDTVVygVTIPHTEGRAGMAAIY-DPERELDLDVFAASLAKVLPAYARPQIIRLLTKV 692
Cdd:PRK12316 2408 KIRGFRIELGEIEARLQAHPAVREAVV--VAQDGASGKQLVAYVVpDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERL 2485
|
570
....*....|....*
gi 939619614 693 DLTGTFKLRKVDLQK 707
Cdd:PRK12316 2486 PLNPNGKLDRKALPK 2500
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
175-662 |
5.19e-14 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 75.47 E-value: 5.19e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 175 NYTVADVFERNVQAHPDKVAVVS------ETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSK 248
Cdd:PRK13295 23 DRTINDDLDACVASCPDKTAVTAvrlgtgAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSR 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 249 IG-VITPLINTnLRGPSLLHSITVAHCSALIY-----GEDFLEAVTDVAKDLPAnltLFQFNNENNNSeteknipqAKNL 322
Cdd:PRK13295 103 IGaVLNPLMPI-FRERELSFMLKHAESKVLVVpktfrGFDHAAMARRLRPELPA---LRHVVVVGGDG--------ADSF 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 323 NALLTTASYEK-PNKTQV------NHHDKLVYIYTSGTTGLPKAAVisHSRYLFIAAGIHYT--MGFQEEDIFYTPLPLY 393
Cdd:PRK13295 171 EALLITPAWEQePDAPAIlarlrpGPDDVTQLIYTSGTTGEPKGVM--HTANTLMANIVPYAerLGLGADDVILMASPMA 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 394 HTAGGIMCMGQSVLFGSTV-------------SIRKK-----FSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQ 455
Cdd:PRK13295 249 HQTGFMYGLMMPVMLGATAvlqdiwdparaaeLIRTEgvtftMASTPFLTDLTRAVKESGRPVSSLRTFLCAGAPIPGAL 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 456 KHRVRLVFGnglrpqiwpqfvqrfniAKVGEFYGATEGnanimnhdntvgaiGFVSRILPKIYPISIIRAD--PDTGEPI 533
Cdd:PRK13295 329 VERARAALG-----------------AKIVSAWGMTEN--------------GAVTLTKLDDPDERASTTDgcPLPGVEV 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 534 RDRNGLCQLCAPNEpgvfIGKIVKGNPSrEFLGYVDEKASAKKIVKDVFKHGDMAFIsgdllvaDEKGYLYFKDRTGDTF 613
Cdd:PRK13295 378 RVVDADGAPLPAGQ----IGRLQVRGCS-NFGGYLKRPQLNGTDADGWFDTGDLARI-------DADGYIRISGRSKDVI 445
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 939619614 614 RWKGENVSTSEVEA------QVSNVA--GYKDT---------VV----YGVTIPHTE---GRAGMAAIYDPER 662
Cdd:PRK13295 446 IRGGENIPVVEIEAllyrhpAIAQVAivAYPDErlgeracafVVprpgQSLDFEEMVeflKAQKVAKQYIPER 518
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
203-643 |
6.78e-14 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 74.87 E-value: 6.78e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 203 TFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYGED 282
Cdd:cd17642 46 SYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFCSKK 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 283 FLEAVTDVAKDLPANLTLFQFNNEN---NNSETEKNIPQakNLNALLTTASYEKPnktQVNHHDKLVYI-YTSGTTGLPK 358
Cdd:cd17642 126 GLQKVLNVQKKLKIIKTIIILDSKEdykGYQCLYTFITQ--NLPPGFNEYDFKPP---SFDRDEQVALImNSSGSTGLPK 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 359 AAVISHSRYLF-IAAGIHYTMGFQ--EEDIFYTPLPLYHTAGGIMCMGQSVLfGSTVSIRKKFSASNYFADCAKYNATIG 435
Cdd:cd17642 201 GVQLTHKNIVArFSHARDPIFGNQiiPDTAILTVIPFHHGFGMFTTLGYLIC-GFRVVLMYKFEEELFLRSLQDYKVQSA 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 436 QYIGE-MARYILATKPSEYDQKHRVRLVFGNG-LRPQIWPQFVQRFNIAKVGEFYGATEGNANIMNHDNTVGAIGFVSRI 513
Cdd:cd17642 280 LLVPTlFAFFAKSTLVDKYDLSNLHEIASGGApLSKEVGEAVAKRFKLPGIRQGYGLTETTSAILITPEGDDKPGAVGKV 359
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 514 LPKIYPISIiraDPDTGepirdrnglcQLCAPNEPGVFIGK---IVKgnpsreflGYV-DEKASAKKIVKDVFKHgdmaf 589
Cdd:cd17642 360 VPFFYAKVV---DLDTG----------KTLGPNERGELCVKgpmIMK--------GYVnNPEATKALIDKDGWLH----- 413
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....
gi 939619614 590 iSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGV 643
Cdd:cd17642 414 -SGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGI 466
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
190-700 |
7.46e-14 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 74.43 E-value: 7.46e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKI-GVITPLintnlrGPSLLhs 268
Cdd:cd17650 1 PDAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAgGAYVPI------DPDYP-- 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 269 itvahcsaliygedfLEAVTDVAKDLPANLTLFQfnnennnsetekniPqaknlnallttasyekpnktqvnhhDKLVY- 347
Cdd:cd17650 73 ---------------AERLQYMLEDSGAKLLLTQ--------------P-------------------------EDLAYv 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISHSRYLFIAAGI--HYTMGFQEE----------DIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIR 415
Cdd:cd17650 99 IYTSGTTGKPKGVMVEHRNVAHAAHAWrrEYELDSFPVrllqmasfsfDVFAGDFARSLLNGGTLVICPDEVKLDPAALY 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 416 KKFSasnyfadcaKYNATIGQYIGEMARYILatkpsEYDQKHRVR------LVFGNGLRPQIWP-----QFVQRFNIAKV 484
Cdd:cd17650 179 DLIL---------KSRITLMESTPALIRPVM-----AYVYRNGLDlsamrlLIVGSDGCKAQDFktlaaRFGQGMRIINS 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 485 gefYGATEgnANImnhDNTVGAIGFVSriLPKIYPISIIRADPDTGEPIRDrnglcQLCAPNEPGV----FIGkivKGNP 560
Cdd:cd17650 245 ---YGVTE--ATI---DSTYYEEGRDP--LGDSANVPIGRPLPNTAMYVLD-----ERLQPQPVGVagelYIG---GAGV 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 561 SREFLGyvDEKASAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVv 640
Cdd:cd17650 307 ARGYLN--RPELTAERFVENPFAPGERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAV- 383
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 641 ygVTIPHTE-GRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd17650 384 --VAVREDKgGEARLCAYVVAAATLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKV 442
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
190-364 |
7.87e-14 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 74.61 E-value: 7.87e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVI-TPLintNLRGPS--LL 266
Cdd:cd12114 1 PDATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAyVPV---DIDQPAarRE 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 267 HSITVAHCSALIygedfleavtdVAKDLPANLtlfqfnnennnseteknIPQAKNLNALLTTASYEK-PNKTQVNHHDKL 345
Cdd:cd12114 78 AILADAGARLVL-----------TDGPDAQLD-----------------VAVFDVLILDLDALAAPApPPPVDVAPDDLA 129
|
170
....*....|....*....
gi 939619614 346 VYIYTSGTTGLPKAAVISH 364
Cdd:cd12114 130 YVIFTSGSTGTPKGVMISH 148
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
177-672 |
8.68e-14 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 74.63 E-value: 8.68e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSET--QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITP 254
Cdd:PLN02330 29 TLPDFVLQDAELYADKVAFVEAVtgKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFS 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 LINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVakDLPAnLTLFQfnnennnseteKNIPQAKNLNALLTTA--SYE 332
Cdd:PLN02330 109 GANPTALESEIKKQAEAAGAKLIVTNDTNYGKVKGL--GLPV-IVLGE-----------EKIEGAVNWKELLEAAdrAGD 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 333 KPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRylFIAAGIHYTMGFQEEDIFYTP----LPLYHTAG--GIMCmgqsv 406
Cdd:PLN02330 175 TSDNEEILQTDLCALPFSSGTTGISKGVMLTHRN--LVANLCSSLFSVGPEMIGQVVtlglIPFFHIYGitGICC----- 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 407 lfgSTVSIRKKFSASNYFADCAKYNATIGQYIgEMARYI------LATKP--SEYD-QKHRVRLVFGNG--LRPQIWPQF 475
Cdd:PLN02330 248 ---ATLRNKGKVVVMSRFELRTFLNALITQEV-SFAPIVppiilnLVKNPivEEFDlSKLKLQAIMTAAapLAPELLTAF 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 476 VQRFNIAKVGEFYGATEGNANIMNHDNTVGAIGFVSR-----ILPKIyPISIIraDPDTGEPIrDRNGLCQLCAPNEpGV 550
Cdd:PLN02330 324 EAKFPGVQVQEAYGLTEHSCITLTHGDPEKGHGIAKKnsvgfILPNL-EVKFI--DPDTGRSL-PKNTPGELCVRSQ-CV 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 551 FIGKivkgnpsreflgYVDEKASAKKIVKDVFKH-GDMAFIsgdllvaDEKGYLYFKDRTGDTFRWKGENVSTSEVEAQV 629
Cdd:PLN02330 399 MQGY------------YNNKEETDRTIDEDGWLHtGDIGYI-------DDDGDIFIVDRIKELIKYKGFQVAPAELEAIL 459
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 939619614 630 SNVAGYKDTVVygVTIPHTEGRAGMAA--IYDP---ERELDLDVFAAS 672
Cdd:PLN02330 460 LTHPSVEDAAV--VPLPDEEAGEIPAAcvVINPkakESEEDILNFVAA 505
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
178-657 |
8.84e-14 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 74.54 E-value: 8.84e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 178 VADVFERNVQAHPDKVAVVSETQRW--TFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITP 254
Cdd:PRK05852 18 IADLVEVAATRLPEAPALVVTADRIaiSYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADlVVVP 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 255 LINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLfqfnnennnseTEKNIPQAKNLNALLTTASYEKP 334
Cdd:PRK05852 98 LDPALPIAEQRVRSQAAGARVVLIDADGPHDRAEPTTRWWPLTVNV-----------GGDSGPSGGTLSVHLDAATEPTP 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 335 NKT--QVNHHDKLVYIYTSGTTGLPKAAVISHSRylfIAAGIH-----YTMGFQeeDIFYTPLPLYHTAGGIMCMGQSVL 407
Cdd:PRK05852 167 ATStpEGLRPDDAMIMFTGGTTGLPKMVPWTHAN---IASSVRaiitgYRLSPR--DATVAVMPLYHGHGLIAALLATLA 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 408 FGSTVSI--RKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQ----RFNi 481
Cdd:PRK05852 242 SGGAVLLpaRGRFSAHTFWDDIKAVGATWYTAVPTIHQILLERAATEPSGRKPAALRFIRSCSAPLTAETAQalqtEFA- 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 482 AKVGEFYGATEGNANImnhdNTVGAIGFVSRILPKIYPISIIRAdpdTGEPIRDRNGLCQLCAPNEpgvfIGKIVKGNPS 561
Cdd:PRK05852 321 APVVCAFGMTEATHQV----TTTQIEGIGQTENPVVSTGLVGRS---TGAQIRIVGSDGLPLPAGA----VGEVWLRGTT 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 562 --REFLGyvDEKASAKKivkdvFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTV 639
Cdd:PRK05852 390 vvRGYLG--DPTITAAN-----FTDG--WLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAA 460
|
490
....*....|....*....
gi 939619614 640 VYGVtiPH-TEGRAGMAAI 657
Cdd:PRK05852 461 VFGV--PDqLYGEAVAAVI 477
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
203-707 |
9.89e-14 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 74.09 E-value: 9.89e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 203 TFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALiyged 282
Cdd:cd05973 2 TFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLV----- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 283 fleaVTDVAkdlpanltlfqfnnennnseteknipqakNLNALLTtasyekpnktqvnhhDKLVYIYTSGTTGLPKAAVI 362
Cdd:cd05973 77 ----VTDAA-----------------------------NRHKLDS---------------DPFVMMFTSGTTGLPKGVPV 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 363 SHSRYLFIAAGIHYTMGFQEEDIFYT------PLPLYHTAGGIMCMGQSVLF-------GSTVSIRKKFSASNYFADCAK 429
Cdd:cd05973 109 PLRALAAFGAYLRDAVDLRPEDSFWNaadpgwAYGLYYAITGPLALGHPTILleggfsvESTWRVIERLGVTNLAGSPTA 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 430 YNATIGqyigemaryilATKPSEYDQKHRVRLVFGNG--LRPQIWPQFVQRFNIAkVGEFYGATE-----GNANIMNHDN 502
Cdd:cd05973 189 YRLLMA-----------AGAEVPARPKGRLRRVSSAGepLTPEVIRWFDAALGVP-IHDHYGQTElgmvlANHHALEHPV 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 503 TVGAIGFVsriLPKiYPISIIraDPDTGEPirdrnglcqlcAPNEPGVfIGKIVKGNPSREFLGYvdEKASAKKIVKDVf 582
Cdd:cd05973 257 HAGSAGRA---MPG-WRVAVL--DDDGDEL-----------GPGEPGR-LAIDIANSPLMWFRGY--QLPDTPAIDGGY- 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 583 khgdmaFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPH----TEGRAGMAAIY 658
Cdd:cd05973 316 ------YLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPErtevVKAFVVLRGGH 389
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 939619614 659 DPERELDlDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRKVDLQK 707
Cdd:cd05973 390 EGTPALA-DELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
203-640 |
1.31e-13 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 73.45 E-value: 1.31e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 203 TFRQVNEHANKVANVLQAQGY-KKGDVVALLLENRAEYVATWLGLSKIG-VITPLintNLRGPS--LLHSITVAHCSALI 278
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAGGvGPGDRVAVLLERSAELVVAILAVLKAGaAYVPL---DPAYPAerLAFILEDAGARLLL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 279 YGEDFLEAVTDVAkdlpanltlfqfnnennnsetEKNIPQAKNLNALLTTASYEKPNKTqVNHHDKLVY-IYTSGTTGLP 357
Cdd:TIGR01733 78 TDSALASRLAGLV---------------------LPVILLDPLELAALDDAPAPPPPDA-PSGPDDLAYvIYTSGSTGRP 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 358 KAAVISH----------SRYLFIAAGIHYTMG------FQEEDIFytpLPLYHtaggimcmGQSVLFGSTVSIRKKFSAS 421
Cdd:TIGR01733 136 KGVVVTHrslvnllawlARRYGLDPDDRVLQFaslsfdASVEEIF---GALLA--------GATLVVPPEDEERDDAALL 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 422 NYFAdcAKYNATIGQYIGEMARYILATKPSEYDqkhRVRLVFGNG--LRPQIWPQFVQRFNIAKVGEFYGATEgnanimn 499
Cdd:TIGR01733 205 AALI--AEHPVTVLNLTPSLLALLAAALPPALA---SLRLVILGGeaLTPALVDRWRARGPGARLINLYGPTE------- 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 500 hdNTVGAIGF-VSR-ILPKIYPISIIRADPDTGEPIRDRNGlcQLCAPNEPG-VFI-GKIVkgnpsreFLGYV-DEKASA 574
Cdd:TIGR01733 273 --TTVWSTATlVDPdDAPRESPVPIGRPLANTRLYVLDDDL--RPVPVGVVGeLYIgGPGV-------ARGYLnRPELTA 341
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 939619614 575 KKIVKDVFKHGDMAFI--SGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVV 640
Cdd:TIGR01733 342 ERFVPDPFAGGDGARLyrTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
169-705 |
2.89e-13 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 73.12 E-value: 2.89e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 169 KRHERLNYTVADVFERNV-QAH--PDKVAV--VSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATW 243
Cdd:PRK05857 4 KKFQAMPQLPSTVLDRVFeQARqqPEAIALrrCDGTSALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 244 LGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYGEdflEAVTDvAKDLPANLtlfqfnnennnseteKNIPQAKNLN 323
Cdd:PRK05857 84 LACAKLGAIAVMADGNLPIAAIERFCQITDPAAALVAP---GSKMA-SSAVPEAL---------------HSIPVIAVDI 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 324 ALLTTASYEKPN----KTQVNH--HDKLVYIYTSGTTGLPKAAVISHSRYLFI-----AAGIHYTMGFQEEDIfYTPLPL 392
Cdd:PRK05857 145 AAVTRESEHSLDaaslAGNADQgsEDPLAMIFTSGTTGEPKAVLLANRTFFAVpdilqKEGLNWVTWVVGETT-YSPLPA 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 393 YHTAG------GIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMaRYILATKPSeydqkhrVRLVFGNG 466
Cdd:PRK05857 224 THIGGlwwiltCLMHGGLCVTGGENTTSLLEILTTNAVATTCLVPTLLSKLVSEL-KSANATVPS-------LRLVGYGG 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 467 LRPQIWP-QFVQRFNIaKVGEFYGATEG--NANIMNHDNtvgaiGFVSRI----LPKIYP-ISIIRADPDTGEPIRDRNG 538
Cdd:PRK05857 296 SRAIAADvRFIEATGV-RTAQVYGLSETgcTALCLPTDD-----GSIVKIeagaVGRPYPgVDVYLAATDGIGPTAPGAG 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 539 lcqlcapnePGVFIGKIVKGNPSrEFLGYVDEKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGE 618
Cdd:PRK05857 370 ---------PSASFGTLWIKSPA-NMLGYWNNPERTAEVLIDGWVN------TGDLLERREDGFFYIKGRSSEMIICGGV 433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 619 NVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGRAGMAAIydPERELDlDVFAASLAKVLPAY--------ARPQIIRLLT 690
Cdd:PRK05857 434 NIAPDEVDRIAEGVSGVREAACYEIPDEEFGALVGLAVV--ASAELD-ESAARALKHTIAARfrresepmARPSTIVIVT 510
|
570
....*....|....*
gi 939619614 691 KVDLTGTFKLRKVDL 705
Cdd:PRK05857 511 DIPRTQSGKVMRASL 525
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
215-643 |
5.04e-13 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 72.18 E-value: 5.04e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 215 ANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTnLRGPSLLHSITVAHCSALIYgedfleAVTDVAKDL 294
Cdd:PLN02574 81 AGLYHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNP-SSSLGEIKKRVVDCSVGLAF------TSPENVEKL 153
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 295 PAnltlfqfnnennNSETEKNIPQAKNLNALLTTAS------YEKPN---KTQVNHHDKLVYIYTSGTTGLPKAAVISHS 365
Cdd:PLN02574 154 SP------------LGVPVIGVPENYDFDSKRIEFPkfyeliKEDFDfvpKPVIKQDDVAAIMYSSGTTGASKGVVLTHR 221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 366 RYL--------FIAAGIHYTmgfQEEDIFYTPLPLYHTAG-GIMCMGQSVLfGSTVSIRKKFSASNYFADCAKYNATIGQ 436
Cdd:PLN02574 222 NLIamvelfvrFEASQYEYP---GSDNVYLAALPMFHIYGlSLFVVGLLSL-GSTIVVMRRFDASDMVKVIDRFKVTHFP 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 437 YIGE-MARYILATKPSEYDQKHRVRLVfGNGLRP---QIWPQFVQRFNIAKVGEFYGATEGNAnimnhdntVGAIGFVSR 512
Cdd:PLN02574 298 VVPPiLMALTKKAKGVCGEVLKSLKQV-SCGAAPlsgKFIQDFVQTLPHVDFIQGYGMTESTA--------VGTRGFNTE 368
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 513 ILPKIYPISIIRadPDTGEPIRDRNGLCqlCAPnePGVfIGKIVKGNPS--REFLgyVDEKASAKKIVKDVFKHgdmafi 590
Cdd:PLN02574 369 KLSKYSSVGLLA--PNMQAKVVDWSTGC--LLP--PGN-CGELWIQGPGvmKGYL--NNPKATQSTIDKDGWLR------ 433
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|...
gi 939619614 591 SGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGV 643
Cdd:PLN02574 434 TGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAV 486
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
183-386 |
5.15e-13 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 72.21 E-value: 5.15e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 183 ERNVQAHPDKVAV------VSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLI 256
Cdd:cd05966 60 DRHLKERGDKVAIiwegdePDQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVV 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHSITVAHCSALIYGEDF--------LEAVTDVA-KDLPA--NLTLFQFNNENNNSETEKNIPqaknLNAL 325
Cdd:cd05966 140 FAGFSAESLADRINDAQCKLVITADGGyrggkvipLKEIVDEAlEKCPSveKVLVVKRTGGEVPMTEGRDLW----WHDL 215
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 939619614 326 LTTASYEKPnKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIH-YTMGFQEEDIF 386
Cdd:cd05966 216 MAKQSPECE-PEWMDSEDPLFILYTSGSTGKPKGVVHTTGGYLLYAATTFkYVFDYHPDDIY 276
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
167-702 |
1.27e-12 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 71.91 E-value: 1.27e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 167 YTKRHERLNYTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGL 246
Cdd:PRK12316 3048 ATAAEYPLERGVHRLFEEQVERTPDAVALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAI 3127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 247 SKIGVITPLINTNLRGPSLLHSItvahcsaliygedfleavtdvaKDLPANLTLFQfnnennnseTEKNIPQAKNLNALL 326
Cdd:PRK12316 3128 LKAGGAYVPLDPEYPEERLAYML----------------------EDSGAQLLLSQ---------SHLRLPLAQGVQVLD 3176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 327 TTA---SYEKPNKTQVNHHDKLVY-IYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPlYHTAGGIMCM 402
Cdd:PRK12316 3177 LDRgdeNYAEANPAIRTMPENLAYvIYTSGSTGKPKGVGIRHSALSNHLCWMQQAYGLGVGDRVLQFTT-FSFDVFVEEL 3255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 403 GQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSEYDQKHR-VRLVFGNGlrpQIWPQFVQRFNI 481
Cdd:PRK12316 3256 FWPLMSGARVVLAGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAHRCTsLKRIVCGG---EALPADLQQQVF 3332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 482 AKVGEF--YGATEGNANIMNHDntvgaigfVSRILPKIYPIsiiradpdtGEPIRDRNglCQLCAPNEPGVFIGKIVKGN 559
Cdd:PRK12316 3333 AGLPLYnlYGPTEATITVTHWQ--------CVEEGKDAVPI---------GRPIANRA--CYILDGSLEPVPVGALGELY 3393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 560 PSREFL--GYVDEKA-SAKKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYK 636
Cdd:PRK12316 3394 LGGEGLarGYHNRPGlTAERFVPDPFVPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVR 3473
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 939619614 637 DTVVYGVtiphtEGRAGMAAIYDPERELDL-DVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL-RK 702
Cdd:PRK12316 3474 EAVVLAV-----DGRQLVAYVVPEDEAGDLrEALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLdRK 3536
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
200-707 |
1.41e-12 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 70.58 E-value: 1.41e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 200 QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIY 279
Cdd:cd05932 5 VEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 280 GEdfLEAVTDVAKDLPANLTLFqfnnennNSETEKNIPQAKNLNALLttASYEKPNKTQVNHHDKLV-YIYTSGTTGLPK 358
Cdd:cd05932 85 GK--LDDWKAMAPGVPEGLISI-------SLPPPSAANCQYQWDDLI--AQHPPLEERPTRFPEQLAtLIYTSGTTGQPK 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 359 AAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVsirkkfsasnYFADcakynaTIGQYI 438
Cdd:cd05932 154 GVMLTFGSFAWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLV----------AFAE------SLDTFV 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 439 GEMaryilatkpseydQKHRVRLVFGnglRPQIWPQF---------VQRFNI---------------------------- 481
Cdd:cd05932 218 EDV-------------QRARPTLFFS---VPRLWTKFqqgvqdkipQQKLNLllkipvvnslvkrkvlkglgldqcrlag 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 482 --------------AKVG----EFYGATEGNA-NIMNH--DNTVGAIGfvsrilpKIYPISIIRADPDTGEPIRdrnglc 540
Cdd:cd05932 282 cgsapvppallewyRSLGlnilEAYGMTENFAySHLNYpgRDKIGTVG-------NAGPGVEVRISEDGEILVR------ 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 541 qlcapnEPGVFIGKivkgnpsreflgYVDEKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRW-KGEN 619
Cdd:cd05932 349 ------SPALMMGY------------YKDPEATAEAFTADGFLR------TGDKGELDADGNLTITGRVKDIFKTsKGKY 404
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 620 VSTSEVEAQVSN--------VAGYKDTVVYGVTIPHTEGRAGMAAIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTK 691
Cdd:cd05932 405 VAPAPIENKLAEhdrvemvcVIGSGLPAPLALVVLSEEARLRADAFARAELEASLRAHLARVNSTLDSHEQLAGIVVVKD 484
|
570 580
....*....|....*....|..
gi 939619614 692 ---VD---LTGTFKLRKVDLQK 707
Cdd:cd05932 485 pwsIDngiLTPTLKIKRNVLEK 506
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
341-627 |
1.78e-12 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 69.43 E-value: 1.78e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 341 HHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCM------GQSVLFGSTVSI 414
Cdd:cd05944 1 SDDVAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLltplasGAHVVLAGPAGY 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 RKKFSASNYFADCAKYNATIGQYIgEMARYILATKPSEYD-QKHRVRLVFGNGLRPQIWPQFVQRFNIaKVGEFYGATEG 493
Cdd:cd05944 81 RNPGLFDNFWKLVERYRITSLSTV-PTVYAALLQVPVNADiSSLRFAMSGAAPLPVELRARFEDATGL-PVVEGYGLTEA 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 494 N-ANIMNHDNTVGAIGFVSRILPKIYpISIIRADPDtGEPIRDrnglcqlCAPNEpgvfIGKIVKGNPSrEFLGYVDEKA 572
Cdd:cd05944 159 TcLVAVNPPDGPKRPGSVGLRLPYAR-VRIKVLDGV-GRLLRD-------CAPDE----VGEICVAGPG-VFGGYLYTEG 224
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*
gi 939619614 573 SAKKIVKDVFkhgdmaFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEA 627
Cdd:cd05944 225 NKNAFVADGW------LNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEE 273
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
181-394 |
4.05e-12 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 69.20 E-value: 4.05e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 181 VFERNVQAHPDKVaVVS-----ETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPL 255
Cdd:cd12119 1 LLEHAARLHGDRE-IVSrthegEVHRYTYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 INTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFQFNNENNNSETEknIPQAKNLNALLTTASYEKPN 335
Cdd:cd12119 80 INPRLFPEQIAYIINHAEDRVVFVDRDFLPLLEAIAPRLPTVEHVVVMTDDAAMPEPA--GVGVLAYEELLAAESPEYDW 157
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 939619614 336 kTQVNHHDKLVYIYTSGTTGLPKAAVISHsRYLFI---AAGIHYTMGFQEEDIFYTPLPLYH 394
Cdd:cd12119 158 -PDFDENTAAAICYTSGTTGNPKGVVYSH-RSLVLhamAALLTDGLGLSESDVVLPVVPMFH 217
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
203-663 |
7.83e-12 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 68.24 E-value: 7.83e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 203 TFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAE----YVATWlglSKIGVITPLintnlrgpslLHSITVahcsali 278
Cdd:cd05914 9 TYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEwgiaFFAIW---TYGAIAVPI----------LAEFTA------- 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 279 ygedflEAVTDVAKDlpanltlfqfnnennnseteknipqaknlnallttasyEKPNKTQVNHHDKL-VYIYTSGTTGLP 357
Cdd:cd05914 69 ------DEVHHILNH--------------------------------------SEAKAIFVSDEDDVaLINYTSGTTGNS 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 358 KAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADcAKY------- 430
Cdd:cd05914 105 KGVMLTYRNIVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPSAKIIAL-AFAqvtptlg 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 431 ------------NATIGQYIGEMARYILATKPSEYDQKHRVR--------------LVFGNGLRPQIwPQFVQRFNIaKV 484
Cdd:cd05914 184 vpvplviekifkMDIIPKLTLKKFKFKLAKKINNRKIRKLAFkkvheafggnikefVIGGAKINPDV-EEFLRTIGF-PY 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 485 GEFYGATEGNANI-MNHDNTV--GAIGFVSrilpKIYPISIIRADPDTGEpirdrnglcqlcapnepgvfiGKI-VKG-N 559
Cdd:cd05914 262 TIGYGMTETAPIIsYSPPNRIrlGSAGKVI----DGVEVRIDSPDPATGE---------------------GEIiVRGpN 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 560 PSREFlgYVDEKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRW-KGENVSTSEVEAQVSNVagykdT 638
Cdd:cd05914 317 VMKGY--YKNPEATAEAFDKDGWFH------TGDLGKIDAEGYLYIRGRKKEMIVLsSGKNIYPEEIEAKINNM-----P 383
|
490 500
....*....|....*....|....*
gi 939619614 639 VVYGVTIPHTEGRAGMAAIYDPERE 663
Cdd:cd05914 384 FVLESLVVVQEKKLVALAYIDPDFL 408
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
203-707 |
1.13e-11 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 67.59 E-value: 1.13e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 203 TFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITpLINTNLRGPSLLHsitvahcSALIYGED 282
Cdd:cd05974 2 SFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVV-IPATTLLTPDDLR-------DRVDRGGA 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 283 FLEAVTDVakdlpanltlfqfnnennnseteknipqaknlnallTTASyekpnktqvnhhDKLVYIYTSGTTGLPKAAVI 362
Cdd:cd05974 74 VYAAVDEN------------------------------------THAD------------DPMLLYFTSGTTSKPKLVEH 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 363 SHSRYLFIAAGIHYTMGFQEEDI----------------FYTPLplyhTAGGIMcmgqsVLFGSTvsirkKFSASNYFAD 426
Cdd:cd05974 106 THRSYPVGHLSTMYWIGLKPGDVhwnisspgwakhawscFFAPW----NAGATV-----FLFNYA-----RFDAKRVLAA 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 427 CAKYNATIGQYIGEMARYILATKPSEYDQKHRVRLVFGNGLRPQIWPQFVQRFNIAkVGEFYGATEGNANIMNHDNTVGA 506
Cdd:cd05974 172 LVRYGVTTLCAPPTVWRMLIQQDLASFDVKLREVVGAGEPLNPEVIEQVRRAWGLT-IRDGYGQTETTALVGNSPGQPVK 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 507 IGFVSRILPKiYPISIIraDPDTGEPirDRNGLCQLCAPNEP-GVFIGkiVKGNPsreflgyvDEKASAKkivkdvfkhG 585
Cdd:cd05974 251 AGSMGRPLPG-YRVALL--DPDGAPA--TEGEVALDLGDTRPvGLMKG--YAGDP--------DKTAHAM---------R 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 586 DMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVygVTIPHTEGRA------GMAAIYD 659
Cdd:cd05974 307 GGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAV--VPSPDPVRLSvpkafiVLRAGYE 384
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 939619614 660 PERELDLDVFAASLAKVLPaYARpqiIRLLTKVDLTGTF--KLRKVDLQK 707
Cdd:cd05974 385 PSPETALEIFRFSRERLAP-YKR---IRRLEFAELPKTIsgKIRRVELRR 430
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
190-700 |
1.15e-11 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 67.39 E-value: 1.15e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLintnlrgpsllhs 268
Cdd:cd17649 1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGgAYVPL------------- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 269 itvahcsaliygedfleavtdvAKDLPANLTLFqfnnennnseteknIPQAKNLNALLTtasyekpnktqvNHHDKLVY- 347
Cdd:cd17649 68 ----------------------DPEYPAERLRY--------------MLEDSGAGLLLT------------HHPRQLAYv 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISH---SRYLFIAAGIhYTMGFQEEDIFYTPLPLyhtAGGIMCMGQSVLFGSTVSIRKK---FSAS 421
Cdd:cd17649 100 IYTSGSTGTPKGVAVSHgplAAHCQATAER-YGLTPGDRELQFASFNF---DGAHEQLLPPLICGACVVLRPDelwASAD 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 422 NYFADCAKYNATIGQ----YIGEMARYILATKPSEYDqkhRVRLVFGNGlrPQIWPQFVQRFNIAKVGEF--YGATEGNA 495
Cdd:cd17649 176 ELAEMVRELGVTVLDlppaYLQQLAEEADRTGDGRPP---SLRLYIFGG--EALSPELLRRWLKAPVRLFnaYGPTEATV 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 496 NIMNHDNTVGAigfvsRILPKIYPIsiiradpdtGEPIRDRNGLC-----QLCAPNEPG-VFIGKivkgnpsrEFL--GY 567
Cdd:cd17649 251 TPLVWKCEAGA-----ARAGASMPI---------GRPLGGRSAYIldadlNPVPVGVTGeLYIGG--------EGLarGY 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 568 VDEKA-SAKKIVKDVF-KHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTI 645
Cdd:cd17649 309 LGRPElTAERFVPDPFgAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDG 388
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 646 PHTEGRAGMAAIYDPERE-LDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd17649 389 AGGKQLVAYVVLRAAAAQpELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKL 444
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
177-704 |
2.70e-11 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 66.30 E-value: 2.70e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGvitpli 256
Cdd:cd17644 1 CIHQLFEEQVERTPDAVAVVFEDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAG------ 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 ntnlrgpsllhsitvahcsaliygedflEAVTDVAKDLPANLTLFqfnnennnseteknIPQAKNLNALLTtasyekpnk 336
Cdd:cd17644 75 ----------------------------GAYVPLDPNYPQERLTY--------------ILEDAQISVLLT--------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 337 tqvNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEED--IFYTPLPLYHTAGGIMcmgQSVLFGSTVSI 414
Cdd:cd17644 104 ---QPENLAYVIYTSGSTGKPKGVMIEHQSLVNLSHGLIKEYGITSSDrvLQFASIAFDVAAEEIY---VTLLSGATLVL 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 415 R--KKFSASNYFADCAK------YNATIGQYigemARYILATKPSEYDQKHRVRLVF--GNGLRPQIWPQFVQrfNIAKV 484
Cdd:cd17644 178 RpeEMRSSLEDFVQYIQqwqltvLSLPPAYW----HLLVLELLLSTIDLPSSLRLVIvgGEAVQPELVRQWQK--NVGNF 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 485 GEF---YGATEGnanimnhdnTVGAIgfVSRIL----PKIYPISIIRADPDTGEPIRDRNglCQLCAPNEPG-VFIG--K 554
Cdd:cd17644 252 IQLinvYGPTEA---------TIAAT--VCRLTqlteRNITSVPIGRPIANTQVYILDEN--LQPVPVGVPGeLHIGgvG 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 555 IVKGNPSREFLgyvdekaSAKKIVKDVFKH--GDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNV 632
Cdd:cd17644 319 LARGYLNRPEL-------TAEKFISHPFNSseSERLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQH 391
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 939619614 633 AGYKDTVVygVTIPHTEGRAGMAAIYDPERELDLDV--FAASLAKVLPAYARPQIIRLLTKVDLTGTfklRKVD 704
Cdd:cd17644 392 NDVKTAVV--IVREDQPGNKRLVAYIVPHYEESPSTveLRQFLKAKLPDYMIPSAFVVLEELPLTPN---GKID 460
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
177-626 |
3.78e-11 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 66.16 E-value: 3.78e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYV-----ATWLGLskigV 251
Cdd:PRK06188 13 TYGHLLVSALKRYPDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLmaigaAQLAGL----R 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 252 ITPLintnlrGP--SL---LHSITVAHCSALI-----YGEDFLEAVTDVAKdLPANLTLfqfnnennnseteKNIPQAKN 321
Cdd:PRK06188 89 RTAL------HPlgSLddhAYVLEDAGISTLIvdpapFVERALALLARVPS-LKHVLTL-------------GPVPDGVD 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 322 LNALLttASYEKPNKTQVNHHDKLVYI-YTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHtAGGIM 400
Cdd:PRK06188 149 LLAAA--AKFGPAPLVAAALPPDIAGLaYTGGTTGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSH-AGGAF 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 401 CMgQSVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMArYILATKPseyDQKHR-----------------VRLVf 463
Cdd:PRK06188 226 FL-PTLLRGGTVIVLAKFDPAEVLRAIEEQRITATFLVPTMI-YALLDHP---DLRTRdlssletvyygaspmspVRLA- 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 464 gNGLRpQIWPQFVQrfniakvgeFYGATEGNANIM-----NHDNTVgaigfVSRILPKIYPISIIRA---DPDtGEPIrd 535
Cdd:PRK06188 300 -EAIE-RFGPIFAQ---------YYGQTEAPMVITylrkrDHDPDD-----PKRLTSCGRPTPGLRVallDED-GREV-- 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 536 rnglcqlcAPNEPgvfiGKI-VKGnP--SREFLGYVDEKASAkkivkdvFKHGDMAfiSGDLLVADEKGYLYFKDRTGDT 612
Cdd:PRK06188 361 --------AQGEV----GEIcVRG-PlvMDGYWNRPEETAEA-------FRDGWLH--TGDVAREDEDGFYYIVDRKKDM 418
|
490
....*....|....
gi 939619614 613 FRWKGENVSTSEVE 626
Cdd:PRK06188 419 IVTGGFNVFPREVE 432
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
184-643 |
1.34e-10 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 64.43 E-value: 1.34e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 184 RNVQAH------------PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG- 250
Cdd:PLN02860 3 NHSQAHicqcltrlatlrGNAVVTISGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGg 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 251 VITPLintNLR-------------GPSLLhsITVAHCSAliYGEDFLEAvtdvakDLPAnLTLFQFNNENNNSETEKnip 317
Cdd:PLN02860 83 IVAPL---NYRwsfeeaksamllvRPVML--VTDETCSS--WYEELQND------RLPS-LMWQVFLESPSSSVFIF--- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 318 qaknLNALLTTASYEKPN--KTQVNHH---DKLVYI-YTSGTTGLPKAAVISHSRYLF-----IAAgihytMGFQEEDIF 386
Cdd:PLN02860 146 ----LNSFLTTEMLKQRAlgTTELDYAwapDDAVLIcFTSGTTGRPKGVTISHSALIVqslakIAI-----VGYGEDDVY 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 387 YTPLPLYHTaGGI---MCMgqsVLFGSTVSIRKKFSASNYFADCAKYNATIGQYIGEMARYILATKPSE--YDQKHRVRL 461
Cdd:PLN02860 217 LHTAPLCHI-GGLssaLAM---LMVGACHVLLPKFDAKAALQAIKQHNVTSMITVPAMMADLISLTRKSmtWKVFPSVRK 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 462 VF--GNGLRPQIWPQFVQRFNIAKVGEFYGATEgnanimnhdnTVGAIGFVSRILPKIYpiSIIRADPDTGEPIRDRNGL 539
Cdd:PLN02860 293 ILngGGSLSSRLLPDAKKLFPNAKLFSAYGMTE----------ACSSLTFMTLHDPTLE--SPKQTLQTVNQTKSSSVHQ 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 540 CQlcapnepGVFIGK--------IVKGNPSRE----------FLGYVDE-KASAKKIVKDVFkhgdmaFISGDLLVADEK 600
Cdd:PLN02860 361 PQ-------GVCVGKpaphvelkIGLDESSRVgriltrgphvMLGYWGQnSETASVLSNDGW------LDTGDIGWIDKA 427
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 939619614 601 GYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGV 643
Cdd:PLN02860 428 GNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGV 470
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
177-410 |
1.40e-10 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 64.61 E-value: 1.40e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDK----VAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYV-ATW---LG--- 245
Cdd:cd05906 11 TLLELLLRAAERGPTKgityIDADGSEEFQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIpAFWacvLAgfv 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 246 --LSKIGVITPLINTNLRgpSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPanltlfqfnnennnseteknIPQAKNLN 323
Cdd:cd05906 91 paPLTVPPTYDEPNARLR--KLRHIWQLLGSPVVLTDAELVAEFAGLETLSG--------------------LPGIRVLS 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 324 ALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMG 403
Cdd:cd05906 149 IEELLDTAADHDLPQSRPDDLALLMLTSGSTGFPKAVPLTHRNILARSAGKIQHNGLTPQDVFLNWVPLDHVGGLVELHL 228
|
....*..
gi 939619614 404 QSVLFGS 410
Cdd:cd05906 229 RAVYLGC 235
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
178-702 |
1.61e-10 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 64.98 E-value: 1.61e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 178 VADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLi 256
Cdd:PRK12316 4553 VHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGgAYVPL- 4631
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHSItvahcsaliygedfleavtdvaKDLPANLTLFQfnnennnSETEKNIPQAKNLNALLTT-----ASY 331
Cdd:PRK12316 4632 DPEYPRERLAYMM----------------------EDSGAALLLTQ-------SHLLQRLPIPDGLASLALDrdedwEGF 4682
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 332 EKPNKTQVNHHDKLVY-IYTSGTTGLPKAAVISHSRYL--FIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMcmgQSVLF 408
Cdd:PRK12316 4683 PAHDPAVRLHPDNLAYvIYTSGSTGRPKGVAVSHGSLVnhLHATGERYELTPDDRVLQFMSFSFDGSHEGLY---HPLIN 4759
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 409 GSTVSIRKK--FSASNYFADCAKYNATIGQYIGEMARYILATKPSEYD-QKHRVRLVFGNGLRPQIWPQFVQRFNIAKVG 485
Cdd:PRK12316 4760 GASVVIRDDslWDPERLYAEIHEHRVTVLVFPPVYLQQLAEHAERDGEpPSLRVYCFGGEAVAQASYDLAWRALKPVYLF 4839
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 486 EFYGATEGNANIMNHDNTVGAIGFVSRIlpkiyPIsiiradpdtGEPIRDRNG--LCQLCAPNEPGVfIGKIVKGNpsrE 563
Cdd:PRK12316 4840 NGYGPTETTVTVLLWKARDGDACGAAYM-----PI---------GTPLGNRSGyvLDGQLNPLPVGV-AGELYLGG---E 4901
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 564 FL--GYVDEKA-SAKKIVKDVF-KHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTV 639
Cdd:PRK12316 4902 GVarGYLERPAlTAERFVPDPFgAPGGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAV 4981
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 640 VYGVTIPHTEGRAGM------AAIYDPERELDL-DVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL-RK 702
Cdd:PRK12316 4982 VIAQEGAVGKQLVGYvvpqdpALADADEAQAELrDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLdRK 5052
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
191-626 |
2.14e-10 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 63.76 E-value: 2.14e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 191 DKVAV----VSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAE-YVATwLGLSKIG-VITPLINTNLRGPs 264
Cdd:PRK04319 59 DKVALryldASRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPElYFAL-LGALKNGaIVGPLFEAFMEEA- 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 265 LLHSITVAHCSALIYGEDFLEAVtdVAKDLPANLTLFqfnnenNNSETEKNIPQAKNLNALLTTASyEKPNKTQVNHHDK 344
Cdd:PRK04319 137 VRDRLEDSEAKVLITTPALLERK--PADDLPSLKHVL------LVGEDVEEGPGTLDFNALMEQAS-DEFDIEWTDREDG 207
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 345 LVYIYTSGTTGLPKAAVISHSrylfiAAGIHYTMG-----FQEEDIFY-TPLP--LYHTAGGIMCmgqSVLFGST-VSIR 415
Cdd:PRK04319 208 AILHYTSGSTGKPKGVLHVHN-----AMLQHYQTGkyvldLHEDDVYWcTADPgwVTGTSYGIFA---PWLNGATnVIDG 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 416 KKFSASNYFADCAKYNATIgQYigemaryilaTKPSEydqkhrVRLVFGNGlrpqiwPQFVQRFN------IAKVGE--- 486
Cdd:PRK04319 280 GRFSPERWYRILEDYKVTV-WY----------TAPTA------IRMLMGAG------DDLVKKYDlsslrhILSVGEpln 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 487 ----FYGATEGNANImnHDN----TVGAIGFVSRILPKIYPISIIRADPDTGEPIRDRNGlcQLCAPNEPGVFigKIVKG 558
Cdd:PRK04319 337 pevvRWGMKVFGLPI--HDNwwmtETGGIMIANYPAMDIKPGSMGKPLPGIEAAIVDDQG--NELPPNRMGNL--AIKKG 410
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 939619614 559 NPSReFLGYVDEKASAKKivkdVFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVE 626
Cdd:PRK04319 411 WPSM-MRGIWNNPEKYES----YFAGD--WYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVE 471
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
167-639 |
2.30e-10 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 63.98 E-value: 2.30e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 167 YTKRHERLN----------YTVADVFERNVQAHPDKV-----------AVVSETQR-----------W-TFRQVNEHANK 213
Cdd:PLN02387 39 YAIRNARFPelvetpwegaTTLAALFEQSCKKYSDKRllgtrklisreFETSSDGRkfeklhlgeyeWiTYGQVFERVCN 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 214 VANVLQAQGYKKGDVVALLLENRAEYVATWLGLSK--IGVITplINTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVA 291
Cdd:PLN02387 119 FASGLVALGHNKEERVAIFADTRAEWLIALQGCFRqnITVVT--IYASLGEEALCHSLNETEVTTVICDSKQLKKLIDIS 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 292 KDLPANLTLFQFNNENNNSETEKNIPQAKNLNALLTTASYEKPNKTQVN---HHDKLVYIYTSGTTGLPKAAVISHSRYL 368
Cdd:PLN02387 197 SQLETVKRVIYMDDEGVDSDSSLSGSSNWTVSSFSEVEKLGKENPVDPDlpsPNDIAVIMYTSGSTGLPKGVMMTHGNIV 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 369 FIAAGIHYTM-GFQEEDIFYTPLPLYH----TAGGIMC-MGQSVLFGSTVSIRkkfSASNYFADCAKYNATigqyigEMA 442
Cdd:PLN02387 277 ATVAGVMTVVpKLGKNDVYLAYLPLAHilelAAESVMAaVGAAIGYGSPLTLT---DTSNKIKKGTKGDAS------ALK 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 443 RYILATKPSEYDQ------------------------KHRVRLVFGN-----GLRPQIWPQFV----------------- 476
Cdd:PLN02387 348 PTLMTAVPAILDRvrdgvrkkvdakgglakklfdiayKRRLAAIEGSwfgawGLEKLLWDALVfkkiravlggrirfmls 427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 477 ---------QRF-NI---AKVGEFYGATE--GNANIMNHDNTvgAIGFVSRILPKIYpISIIRAdPDTGEPIRDRNglcq 541
Cdd:PLN02387 428 ggaplsgdtQRFiNIclgAPIGQGYGLTEtcAGATFSEWDDT--SVGRVGPPLPCCY-VKLVSW-EEGGYLISDKP---- 499
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 542 lcAPNepgvfiGKIVKGNPSREfLGYVDEKASAKKIVKdVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWK-GENV 620
Cdd:PLN02387 500 --MPR------GEIVIGGPSVT-LGYFKNQEKTDEVYK-VDERGMRWFYTGDIGQFHPDGCLEIIDRKKDIVKLQhGEYV 569
|
570
....*....|....*....
gi 939619614 621 STSEVEAQVSnVAGYKDTV 639
Cdd:PLN02387 570 SLGKVEAALS-VSPYVDNI 587
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
175-626 |
4.34e-10 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 62.94 E-value: 4.34e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 175 NYTVAD---VFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGV 251
Cdd:PLN02479 16 NYTALTplwFLERAAVVHPTRKSVVHGSVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGA 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 252 ITPLINTNLRGPS----LLHSITvahcSALIYGEDFLEAVTDVAKDL---------PANLTLFQFNNENNNSETEKNIPQ 318
Cdd:PLN02479 96 VVNCVNIRLNAPTiaflLEHSKS----EVVMVDQEFFTLAEEALKILaekkkssfkPPLLIVIGDPTCDPKSLQYALGKG 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 319 AKNLNALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGG 398
Cdd:PLN02479 172 AIEYEKFLETGDPEFAWKPPADEWQSIALGYTSGTTASPKGVVLHHRGAYLMALSNALIWGMNEGAVYLWTLPMFHCNGW 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 399 IMCMGQSVLFGSTVSIRkKFSASNYFADCAKY----------------NATIGQYIGEMARYI------LATKPSEY--- 453
Cdd:PLN02479 252 CFTWTLAALCGTNICLR-QVTAKAIYSAIANYgvthfcaapvvlntivNAPKSETILPLPRVVhvmtagAAPPPSVLfam 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 454 -DQKHRVRLVFG----------NGLRPQiW----PQFVQRFNiAKVGEFYGATEGnanimnhdntvgaigfvsrilpkiy 518
Cdd:PLN02479 331 sEKGFRVTHTYGlsetygpstvCAWKPE-WdslpPEEQARLN-ARQGVRYIGLEG------------------------- 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 519 pISIIraDPDTGEPIrdrnglcqlcaPNEpGVFIGKIV-KGNPSREflGYV-DEKASAkkivkDVFKHGdmAFISGDLLV 596
Cdd:PLN02479 384 -LDVV--DTKTMKPV-----------PAD-GKTMGEIVmRGNMVMK--GYLkNPKANE-----EAFANG--WFHSGDLGV 439
|
490 500 510
....*....|....*....|....*....|
gi 939619614 597 ADEKGYLYFKDRTGDTFRWKGENVSTSEVE 626
Cdd:PLN02479 440 KHPDGYIEIKDRSKDIIISGGENISSLEVE 469
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
173-400 |
7.42e-10 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 62.29 E-value: 7.42e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 173 RLNYTvadvfeRNVQAH---PDKVAVVS----ETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLG 245
Cdd:cd05943 69 RLNYA------ENLLRHadaDDPAAIYAaedgERTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLA 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 246 LSKIGVI----TPliNTNLRG---------PSLLHSitvahCSALIYG---EDFLEAVTDVAKDLPANL-TLFQFNNENN 308
Cdd:cd05943 143 TASIGAIwsscSP--DFGVPGvldrfgqiePKVLFA-----VDAYTYNgkrHDVREKVAELVKGLPSLLaVVVVPYTVAA 215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 309 NSETEKNIPQAKNLNALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFI---AAGIHYTMGFQEEDI 385
Cdd:cd05943 216 GQPDLSKIAKALTLEDFLATGAAGELEFEPLPFDHPLYILYSSGTTGLPKCIVHGAGGTLLQhlkEHILHCDLRPGDRLF 295
|
250
....*....|....*
gi 939619614 386 FYTplplyhTAGGIM 400
Cdd:cd05943 296 YYT------TCGWMM 304
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
201-642 |
9.70e-10 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 61.68 E-value: 9.70e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 201 RWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYG 280
Cdd:cd05915 24 RTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLLFD 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 281 EDFLeAVTDVAKDLPANLTlfqfnnennnsetEKNIPQAK--NLNALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPK 358
Cdd:cd05915 104 PNLL-PLVEAIRGELKTVQ-------------HFVVMDEKapEGYLAYEEALGEEADPVRVPERAACGMAYTTGTTGLPK 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 359 AAVISH-SRYL-FIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATigQ 436
Cdd:cd05915 170 GVVYSHrALVLhSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGPRLDPASLVELFDGEGVT--F 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 437 YIGEMARYILATKPSEYDQKH---RVRLVFGNGLRPQIWPQfVQRFNIAKVGEFYGATEgnanimnhdntVGAIGFVSRI 513
Cdd:cd05915 248 TAGVPTVWLALADYLESTGHRlktLRRLVVGGSAAPRSLIA-RFERMGVEVRQGYGLTE-----------TSPVVVQNFV 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 514 LPKIYPISiiradPDTGEPIRDRNGL------------CQLCAPNEPGVFIGKIVKGNPSREflGYVDEKASAKkivKDV 581
Cdd:cd05915 316 KSHLESLS-----EEEKLTLKAKTGLpiplvrlrvadeEGRPVPKDGKALGEVQLKGPWITG--GYYGNEEATR---SAL 385
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 582 FKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYG 642
Cdd:cd05915 386 TPDG--FFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVA 444
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
177-402 |
1.31e-09 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 61.38 E-value: 1.31e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLGLSKIGVItpL 255
Cdd:PRK12492 25 SVVEVFERSCKKFADRPAFSNLGVTLSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLI--V 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 INTN--LRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKD--------------LPA--NLTLFQFNNENNNSETEKNIP 317
Cdd:PRK12492 103 VNTNplYTAREMRHQFKDSGARALVYLNMFGKLVQEVLPDtgieylieakmgdlLPAakGWLVNTVVDKVKKMVPAYHLP 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 318 QAKNLNALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEED----------IFY 387
Cdd:PRK12492 183 QAVPFKQALRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVRACLSQLGPDgqplmkegqeVMI 262
|
250
....*....|....*....
gi 939619614 388 TPLPLYH----TAgGIMCM 402
Cdd:PRK12492 263 APLPLYHiyafTA-NCMCM 280
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
201-394 |
5.38e-09 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 59.38 E-value: 5.38e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 201 RWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSALIYG 280
Cdd:PRK06018 39 RTTYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVITD 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 281 EDFLEAVTDVAKDLPANLTLFQFnnennnsETEKNIPQAKNLNALlttaSYEKpnktQVNHHDKLV------------YI 348
Cdd:PRK06018 119 LTFVPILEKIADKLPSVERYVVL-------TDAAHMPQTTLKNAV----AYEE----WIAEADGDFawktfdentaagMC 183
|
170 180 190 200
....*....|....*....|....*....|....*....|....*...
gi 939619614 349 YTSGTTGLPKAAVISH-SRYLF-IAAGIHYTMGFQEEDIFYTPLPLYH 394
Cdd:PRK06018 184 YTSGTTGDPKGVLYSHrSNVLHaLMANNGDALGTSAADTMLPVVPLFH 231
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
190-700 |
9.53e-09 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 58.42 E-value: 9.53e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG-VITPLintnlrGPSllhs 268
Cdd:cd17652 1 PDAPAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGaAYLPL------DPA---- 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 269 itvahcsaliYGEDFLEA-VTDVAkdlPAnltlfqfnnennnseteknipqaknlnALLTTAsyekpnktqvnhhDKLVY 347
Cdd:cd17652 71 ----------YPAERIAYmLADAR---PA---------------------------LLLTTP-------------DNLAY 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 -IYTSGTTGLPKAAVISHsrylfiaAGIHYTMGFQEEDIFYTPlplyhtaggimcmGQSVLFGSTVSirkkFSASnyFAD 426
Cdd:cd17652 98 vIYTSGSTGRPKGVVVTH-------RGLANLAAAQIAAFDVGP-------------GSRVLQFASPS----FDAS--VWE 151
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 427 -CAKY--NATI----------GQYIGEMAR-----------YILATKPSEYDQKHRVRLVFGNGLRPQI---WPQFVQRF 479
Cdd:cd17652 152 lLMALlaGATLvlapaeellpGEPLADLLRehrithvtlppAALAALPPDDLPDLRTLVVAGEACPAELvdrWAPGRRMI 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 480 NIakvgefYGATEgnanimnhdNTVGAIgfVSRILPKIYPISIIRADPDTGEPIRDRNglcqlCAPNEPGVfIGKIVKGN 559
Cdd:cd17652 232 NA------YGPTE---------TTVCAT--MAGPLPGGGVPPIGRPVPGTRVYVLDAR-----LRPVPPGV-PGELYIAG 288
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 560 PS--ReflGYVDEKA-SAKKIVKDVFKH-GDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGY 635
Cdd:cd17652 289 AGlaR---GYLNRPGlTAERFVADPFGApGSRMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGV 365
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 939619614 636 KDTVVygvtIPHTEGRAG--MAAIYDPERELDLDVFA--ASLAKVLPAYARPQIIRLLTKVDLTGTFKL 700
Cdd:cd17652 366 AEAVV----VVRDDRPGDkrLVAYVVPAPGAAPTAAElrAHLAERLPGYMVPAAFVVLDALPLTPNGKL 430
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
197-403 |
9.79e-09 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 58.38 E-value: 9.79e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 197 SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVAHCSA 276
Cdd:cd17639 1 GEYKYMSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIHSLNETECSA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 277 LIygedfleavTDVAKDlpanltlfqfnnennnseteknipqaknlnallttasyekpnktqvnhhDKLVYIYTSGTTGL 356
Cdd:cd17639 81 IF---------TDGKPD-------------------------------------------------DLACIMYTSGSTGN 102
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 939619614 357 PKAAVISHSRylfIAAGIHyTMGFQ------EEDIFYTPLPLYH-----------TAGGIMCMG 403
Cdd:cd17639 103 PKGVMLTHGN---LVAGIA-GLGDRvpellgPDDRYLAYLPLAHifelaaenvclYRGGTIGYG 162
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
348-644 |
1.40e-08 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 58.40 E-value: 1.40e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTaggimcmgqsvlFGSTVS----IRKKFSASnY 423
Cdd:PRK08633 788 IFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFFHS------------FGLTVTlwlpLLEGIKVV-Y 854
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 424 FAD----------CAKYNATIgqyigemaryILATKP--SEYDQKHRV--------RLVFGNG--LRPQIWPQFVQRFNI 481
Cdd:PRK08633 855 HPDptdalgiaklVAKHRATI----------LLGTPTflRLYLRNKKLhplmfaslRLVVAGAekLKPEVADAFEEKFGI 924
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 482 aKVGEFYGATE----GNANIMN---------HDNTVGAIGfvsriLPkiYPISIIR-ADPDTGEPirdrnglcqlCAPNE 547
Cdd:PRK08633 925 -RILEGYGATEtspvASVNLPDvlaadfkrqTGSKEGSVG-----MP--LPGVAVRiVDPETFEE----------LPPGE 986
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 548 PGVFIgkiVKGnpSREFLGYVDEKASAKKIVKDVFKHGdmAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEA 627
Cdd:PRK08633 987 DGLIL---IGG--PQVMKGYLGDPEKTAEVIKDIDGIG--WYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEE 1059
|
330
....*....|....*..
gi 939619614 628 QVSNVAGyKDTVVYGVT 644
Cdd:PRK08633 1060 ELAKALG-GEEVVFAVT 1075
|
|
| alpha_am_amid |
TIGR03443 |
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ... |
180-254 |
1.41e-08 |
|
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.
Pssm-ID: 274582 [Multi-domain] Cd Length: 1389 Bit Score: 58.54 E-value: 1.41e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 180 DVFERNVQAHPDKVAVV---------SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG 250
Cdd:TIGR03443 240 DIFADNAEKHPDRTCVVetpsfldpsSKTRSFTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAG 319
|
....*...
gi 939619614 251 ----VITP 254
Cdd:TIGR03443 320 atfsVIDP 327
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
190-700 |
2.24e-08 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 57.02 E-value: 2.24e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 190 PDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGD-VVALLLENRAEYVATWLGLSKIGVITPLINtnlrgPSllhs 268
Cdd:cd17648 1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAEIRPDdLVGLVLDKSELMIIAILAVWKAGAAYVPID-----PS---- 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 269 itvahcsaliYGEDFLEAVTDVAkdlpanltlfqfnnennnseteknipQAKnlnALLTTASyekpnktqvnhhdKLVY- 347
Cdd:cd17648 72 ----------YPDERIQFILEDT--------------------------GAR---VVITNST-------------DLAYa 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISH-----------SRYLFIAAGIH-------YTMGFQEEDifytplplyhtaggiMCMgqSVLFG 409
Cdd:cd17648 100 IYTSGTTGKPKGVLVEHgsvvnlrtslsERYFGRDNGDEavlffsnYVFDFFVEQ---------------MTL--ALLNG 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 410 STVSIrkkFSASNYFADCAKYnatigQYI-GEMARYILATkPS---EYD-----QKHRVRLVfGNGLRPQIWPQFVQRFN 480
Cdd:cd17648 163 QKLVV---PPDEMRFDPDRFY-----AYInREKVTYLSGT-PSvlqQYDlarlpHLKRVDAA-GEEFTAPVFEKLRSRFA 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 481 IAKVGEfYGATEgnANIMNHDntvgaigfvsrilpKIYPISiIRADPDTGEPIrdRNGLCQLCAPNEPGVFIGKI----- 555
Cdd:cd17648 233 GLIINA-YGPTE--TTVTNHK--------------RFFPGD-QRFDKSLGRPV--RNTKCYVLNDAMKRVPVGAVgelyl 292
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 556 -----VKGNPSREFLG--------YVDEKASAKKIVKDVFKHGDMAFISGDllvadekGYLYFKDRTGDTFRWKGENVST 622
Cdd:cd17648 293 ggdgvARGYLNRPELTaerflpnpFQTEQERARGRNARLYKTGDLVRWLPS-------GELEYLGRNDFQVKIRGQRIEP 365
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 623 SEVEAQVSNVAGYKDTVV---YGVTIPHTEGRAGMAAIYDPEREL--DLDVFAASLAKvLPAYARPQIIRLLTKVDLTGT 697
Cdd:cd17648 366 GEVEAALASYPGVRECAVvakEDASQAQSRIQKYLVGYYLPEPGHvpESDLLSFLRAK-LPRYMVPARLVRLEGIPVTIN 444
|
...
gi 939619614 698 FKL 700
Cdd:cd17648 445 GKL 447
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
139-364 |
3.51e-08 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 56.73 E-value: 3.51e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 139 PGWRWFyiAGAtasrdltalwayikllrytkrheRLNYTvadvfeRNV--QAHPDKVAVVS-----ETQRWTFRQVNEHA 211
Cdd:PRK03584 76 PGARWF--PGA-----------------------RLNYA------ENLlrHRRDDRPAIIFrgedgPRRELSWAELRRQV 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 212 NKVANVLQAQGYKKGDVVALLLENRAEYV-----ATWLG---------------LSKIGVITplintnlrgPSLLhsITV 271
Cdd:PRK03584 125 AALAAALRALGVGPGDRVAAYLPNIPETVvamlaTASLGaiwsscspdfgvqgvLDRFGQIE---------PKVL--IAV 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 272 AHCSaliYG---EDFLEAVTDVAKDLPaNLTLFQFNNENNNSETEKNIPQAKNLNALLTTASYEKPNKTQVNHHDKLvYI 348
Cdd:PRK03584 194 DGYR---YGgkaFDRRAKVAELRAALP-SLEHVVVVPYLGPAAAAAALPGALLWEDFLAPAEAAELEFEPVPFDHPL-WI 268
|
250
....*....|....*..
gi 939619614 349 -YTSGTTGLPKAAVISH 364
Cdd:PRK03584 269 lYSSGTTGLPKCIVHGH 285
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
348-702 |
4.43e-08 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 55.59 E-value: 4.43e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHT----AGGIMCMgqsvLFGSTVSIRKKFSASNY 423
Cdd:cd17638 6 MFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYLIINPFFHTfgykAGIVACL----LTGATVVPVAVFDVDAI 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 424 FADCAKYNATIGQYIGEMARYILATKPSEYDQKHRVRL-VFGNGLRPqiwPQFVQR----FNIAKVGEFYGATEGNANIM 498
Cdd:cd17638 82 LEAIERERITVLPGPPTLFQSLLDHPGRKKFDLSSLRAaVTGAATVP---VELVRRmrseLGFETVLTAYGLTEAGVATM 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 499 NHD--------NTVGaigfvsrilpkiypisiiRADPDTGEPIRDRnglcqlcapnepgvfiGKIVKGNPSrEFLGYV-D 569
Cdd:cd17638 159 CRPgddaetvaTTCG------------------RACPGFEVRIADD----------------GEVLVRGYN-VMQGYLdD 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 570 EKASAKKIVKDVFKHgdmafiSGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTiPHTE 649
Cdd:cd17638 204 PEATAEAIDADGWLH------TGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVP-DERM 276
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 939619614 650 GRAGMA-AIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKLRK 702
Cdd:cd17638 277 GEVGKAfVVARPGVTLTEEDVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
180-251 |
8.24e-08 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 55.38 E-value: 8.24e-08
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 939619614 180 DVFERnvQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGV 251
Cdd:PRK10946 29 DILTR--HAASDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGV 98
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
181-365 |
1.52e-07 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 55.35 E-value: 1.52e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 181 VFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKI-GVITPLintn 259
Cdd:PRK12316 516 LFEEQVERTPEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAgGAYVPL---- 591
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 260 lrGPSllhsitvahcsaliYGEDFLEAVTDvakDLPANLTLFQfnnennnSETEKNIPQAKNLNAL------LTTASYEK 333
Cdd:PRK12316 592 --DPE--------------YPAERLAYMLE---DSGVQLLLSQ-------SHLGRKLPLAAGVQVLdldrpaAWLEGYSE 645
|
170 180 190
....*....|....*....|....*....|...
gi 939619614 334 PNKTQVNHHDKLVY-IYTSGTTGLPKAAVISHS 365
Cdd:PRK12316 646 ENPGTELNPENLAYvIYTSGSTGKPKGAGNRHR 678
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
184-252 |
1.60e-07 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 54.76 E-value: 1.60e-07
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 939619614 184 RNVQAHPDKVAVV------SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVI 252
Cdd:PRK00174 75 RHLKTRGDKVAIIwegddpGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAV 149
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
346-426 |
2.37e-07 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 54.29 E-value: 2.37e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 346 VYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEED----IFYTPLPLYHTAGGIMCMGQSVLFGSTVsirkkfsas 421
Cdd:cd05933 154 TLIYTSGTTGMPKGVMLSHDNITWTAKAASQHMDLRPATvgqeSVVSYLPLSHIAAQILDIWLPIKVGGQV--------- 224
|
....*
gi 939619614 422 nYFAD 426
Cdd:cd05933 225 -YFAQ 228
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
186-441 |
4.95e-07 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 53.08 E-value: 4.95e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 186 VQAHPDkvavvsETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYV----ATWL-GLSKIGVITPLINTNL 260
Cdd:PRK07768 20 VTGEPD------APVRHTWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIAptaqGLWMrGASLTMLHQPTPRTDL 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 261 --------RGPSLLHSitvahcSALIYGEDFLEAVTDVAKDLPANLTLfqfnnennnseteknipqaknlNALLTTASYE 332
Cdd:PRK07768 94 avwaedtlRVIGMIGA------KAVVVGEPFLAAAPVLEEKGIRVLTV----------------------ADLLAADPID 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 333 KPnktQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEE-DIFYTPLPLYHTAGGI------MCMGQS 405
Cdd:PRK07768 146 PV---ETGEDDLALMQLTSGSTGSPKAVQITHGNLYANAEAMFVAAEFDVEtDVMVSWLPLFHDMGMVgfltvpMYFGAE 222
|
250 260 270
....*....|....*....|....*....|....*.
gi 939619614 406 VLFGSTVsirkkfsasNYFADCAKYNATIGQYIGEM 441
Cdd:PRK07768 223 LVKVTPM---------DFLRDPLLWAELISKYRGTM 249
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
177-394 |
6.28e-07 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 52.79 E-value: 6.28e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPD------KVAV---VSEtQRW-TFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGL 246
Cdd:PLN02736 45 TLHDNFVYAVETFRDykylgtRIRVdgtVGE-YKWmTYGEAGTARTAIGSGLVQHGIPKGACVGLYFINRPEWLIVDHAC 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 247 SKIGVIT-PLINTnlRGPSLLHSItVAHCSaliygedfLEAVTDVAKDLPanlTLFQFNNENNNSETEKNIPQA-KNLNA 324
Cdd:PLN02736 124 SAYSYVSvPLYDT--LGPDAVKFI-VNHAE--------VAAIFCVPQTLN---TLLSCLSEIPSVRLIVVVGGAdEPLPS 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 325 LLTTA-----SYEK-------------PNKTqvnhHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIF 386
Cdd:PLN02736 190 LPSGTgveivTYSKllaqgrsspqpfrPPKP----EDVATICYTSGTTGTPKGVVLTHGNLIANVAGSSLSTKFYPSDVH 265
|
....*...
gi 939619614 387 YTPLPLYH 394
Cdd:PLN02736 266 ISYLPLAH 273
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
193-411 |
9.78e-07 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 52.29 E-value: 9.78e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 193 VAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLLHSITVA 272
Cdd:PTZ00216 113 VTHFNETRYITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRET 192
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 273 HCSALIYGedfleavtdvAKDLPANLTLFQfnnennnsetEKNIPQAK-----NLNALLTTASY--------EKPNKTQV 339
Cdd:PTZ00216 193 ECKAIVCN----------GKNVPNLLRLMK----------SGGMPNTTiiyldSLPASVDTEGCrlvawtdvVAKGHSAG 252
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 340 NHH--------DKLVYI-YTSGTTGLPKAAVISHSRylfIAAGIHyTMG---------FQEEDIFYTPLPLYH----TAG 397
Cdd:PTZ00216 253 SHHplnipennDDLALImYTSGTTGDPKGVMHTHGS---LTAGIL-ALEdrlndligpPEEDETYCSYLPLAHimefGVT 328
|
250
....*....|....*
gi 939619614 398 GIMCM-GQSVLFGST 411
Cdd:PTZ00216 329 NIFLArGALIGFGSP 343
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
178-643 |
1.35e-06 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 51.69 E-value: 1.35e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 178 VADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLGLSKIGVItpLI 256
Cdd:PRK05677 26 IQAVLKQSCQRFADKPAFSNLGKTLTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLI--VV 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTN--LRGPSLLHSITVAHCSALI-------YGEDFLEA-------VTDVAKDLP--ANLTLFQFNNENNNSETEKNIPQ 318
Cdd:PRK05677 104 NTNplYTAREMEHQFNDSGAKALVclanmahLAEKVLPKtgvkhviVTEVADMLPplKRLLINAVVKHVKKMVPAYHLPQ 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 319 AKNLNALLTTASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEED---IFYTPLPLYHT 395
Cdd:PRK05677 184 AVKFNDALAKGAGQPVTEANPQADDVAVLQYTGGTTGVAKGAMLTHRNLVANMLQCRALMGSNLNEgceILIAPLPLYHI 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 396 -AGGIMCMGQSVLFGSTVSIRKKFSASNYFADCAKYNATigQYIGeMARYILATKPSEYDQK---HRVRLVFGNGLRPQI 471
Cdd:PRK05677 264 yAFTFHCMAMMLIGNHNILISNPRDLPAMVKELGKWKFS--GFVG-LNTLFVALCNNEAFRKldfSALKLTLSGGMALQL 340
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 472 -----WpqfvQRFNIAKVGEFYGATEGN----ANIMNHdNTVGAIGfvsriLPkiYPISIIRADPDTGE--PIRDRNGLC 540
Cdd:PRK05677 341 ataerW----KEVTGCAICEGYGMTETSpvvsVNPSQA-IQVGTIG-----IP--VPSTLCKVIDDDGNelPLGEVGELC 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 541 qlcapnepgvfigkiVKGnpSREFLGYVDEKASAKKIVKD--VFKHGDMAFISGDllvadekGYLYFKDRTGDTFRWKGE 618
Cdd:PRK05677 409 ---------------VKG--PQVMKGYWQRPEATDEILDSdgWLKTGDIALIQED-------GYMRIVDRKKDMILVSGF 464
|
490 500
....*....|....*....|....*
gi 939619614 619 NVSTSEVEAQVSNVAGYKDTVVYGV 643
Cdd:PRK05677 465 NVYPNELEDVLAALPGVLQCAAIGV 489
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
177-702 |
1.66e-06 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 51.97 E-value: 1.66e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLI 256
Cdd:PRK10252 459 TLSALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPL 538
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 257 NTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKdlpanLTLFQFnnennnseteknipqaknlNALLTTASYeKPNK 336
Cdd:PRK10252 539 DTGYPDDRLKMMLEDARPSLLITTADQLPRFADVPD-----LTSLCY-------------------NAPLAPQGA-APLQ 593
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 337 TQVNHHDKLVyIYTSGTTGLPKAAVISH----SRYLFIAAgiHYTMGFQEEDIFYTP-----------LPLyhTAGGIMC 401
Cdd:PRK10252 594 LSQPHHTAYI-IFTSGSTGRPKGVMVGQtaivNRLLWMQN--HYPLTADDVVLQKTPcsfdvsvweffWPF--IAGAKLV 668
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 402 MGQSVLFGSTVSIRKKFsasnyfadcAKYNATIGQYIGEMARYILATKPSE--YDQKHRVRLVF--GNGLRPQIWPQFVQ 477
Cdd:PRK10252 669 MAEPEAHRDPLAMQQFF---------AEYGVTTTHFVPSMLAAFVASLTPEgaRQSCASLRQVFcsGEALPADLCREWQQ 739
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 478 RFNiAKVGEFYGATEGNANIMNHDNTVGAIGFV-SRILPKIYPISiiradpDTGEPIRDrnglcQLCAPNEPGV----FI 552
Cdd:PRK10252 740 LTG-APLHNLYGPTEAAVDVSWYPAFGEELAAVrGSSVPIGYPVW------NTGLRILD-----ARMRPVPPGVagdlYL 807
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 553 GKIvkgNPSREFLGYVDEKASakKIVKDVFKHGDMAFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSNV 632
Cdd:PRK10252 808 TGI---QLAQGYLGRPDLTAS--RFIADPFAPGERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQAL 882
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 633 AGYKDTVVYGVTIPHTEGRAG---------MAAIYDPereLDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFKL-RK 702
Cdd:PRK10252 883 PDVEQAVTHACVINQAAATGGdarqlvgylVSQSGLP---LDTSALQAQLRERLPPHMVPVVLLQLDQLPLSANGKLdRK 959
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
164-362 |
2.29e-06 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 51.32 E-value: 2.29e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 164 LLRYTKRHER---LNYTVADVFERNVQAHPDKVAVVSETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYV 240
Cdd:PRK05691 3705 LLDGCNRSERdypLEQSYVRLFEAQVAAHPQRIAASCLDQQWSYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLL 3784
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 241 ATWLGLSKIGV----ITP-LINTNLRGPSLLHSITVAHCSA--LIYGEDFLEAVTDVAKdlpanltlfqfnnennnsete 313
Cdd:PRK05691 3785 GMIVGSFKAGAgylpLDPgLPAQRLQRIIELSRTPVLVCSAacREQARALLDELGCANR--------------------- 3843
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*...
gi 939619614 314 kniPQaknlnaLLTtasYEKPNKTQVNHH--------DKLVY-IYTSGTTGLPKAAVI 362
Cdd:PRK05691 3844 ---PR------LLV---WEEVQAGEVASHnpgiysgpDNLAYvIYTSGSTGLPKGVMV 3889
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
624-699 |
4.74e-06 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 44.84 E-value: 4.74e-06
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 939619614 624 EVEAQVSNVAGYKDTVVYGVTIPhTEGRAGMA-AIYDPERELDLDVFAASLAKVLPAYARPQIIRLLTKVDLTGTFK 699
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDE-LKGEAPVAfVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
177-707 |
1.77e-05 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 48.24 E-value: 1.77e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 177 TVADVFERNVQAHPDKVAVV---SETQRWTFRQVNEHANKVANVLQAQ-GYKKGDVVALLLENRAEYVATWLGLSKIG-V 251
Cdd:PRK05620 11 SLTRILEYGSTVHGDTTVTTwggAEQEQTTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGaV 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 252 ITPLiNTNLRGPSLLHSITVAHCSALIYGEDFLEAVTDVAKDLPANLTLFqFNNENNNSETEKNIP---QAKNLNALLT- 327
Cdd:PRK05620 91 FNPL-NKQLMNDQIVHIINHAEDEVIVADPRLAEQLGEILKECPCVRAVV-FIGPSDADSAAAHMPegiKVYSYEALLDg 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 328 -TASYEKPnktQVNHHDKLVYIYTSGTTGLPKAAVISHsRYLFI-AAGIHYT--MGFQEEDIFYTPLPLYHT------AG 397
Cdd:PRK05620 169 rSTVYDWP---ELDETTAAAICYSTGTTGAPKGVVYSH-RSLYLqSLSLRTTdsLAVTHGESFLCCVPIYHVlswgvpLA 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 398 GIMCMGQSVLFGSTVSIRkkfSASNYFADCAKYNA----TIgqYIGEMARYilATKPSEydqkhRVRL----VFGNGLRP 469
Cdd:PRK05620 245 AFMSGTPLVFPGPDLSAP---TLAKIIATAMPRVAhgvpTL--WIQLMVHY--LKNPPE-----RMSLqeiyVGGSAVPP 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 470 QIWPQFVQRFNIaKVGEFYGATEgnanimnhdntVGAIGFVS--------------RILPKIYPISIIRADPDTGEPIR- 534
Cdd:PRK05620 313 ILIKAWEERYGV-DVVHVWGMTE-----------TSPVGTVArppsgvsgearwayRVSQGRFPASLEYRIVNDGQVMEs 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 535 -DRN-GLCQLCAPNEPGVFIGKIVK--GNPSREFLGYVDEKASAKKIVKDVFKHGDMAFISGDllvadekGYLYFKDRTG 610
Cdd:PRK05620 381 tDRNeGEIQVRGNWVTASYYHSPTEegGGAASTFRGEDVEDANDRFTADGWLRTGDVGSVTRD-------GFLTIHDRAR 453
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 611 DTFRWKGENVSTSEVEAQVSNVAGYKDTVVYGVTIPHTEGR----AGMAAIYDPERELDLDVfAASLAKVLPAYARPQII 686
Cdd:PRK05620 454 DVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERplavTVLAPGIEPTRETAERL-RDQLRDRLPNWMLPEYW 532
|
570 580
....*....|....*....|.
gi 939619614 687 RLLTKVDLTGTFKLRKVDLQK 707
Cdd:PRK05620 533 TFVDEIDKTSVGKFDKKDLRQ 553
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
200-412 |
1.87e-05 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 48.19 E-value: 1.87e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 200 QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLRGPSLlhSITVAHCSA-LI 278
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEV--AYLLNYTGArVV 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 279 YGED--FLEAVTDVAKDLPANLTLFQFNNENNNSETEKNIPQAKNLNALLTTASYEKPNK-----TQVNHHDKLVYIYTS 351
Cdd:cd17641 88 IAEDeeQVDKLLEIADRIPSVRYVIYCDPRGMRKYDDPRLISFEDVVALGRALDRRDPGLyerevAAGKGEDVAVLCTTS 167
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939619614 352 GTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTV 412
Cdd:cd17641 168 GTTGKPKLAMLSHGNFLGHCAAYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCGFIV 228
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
183-627 |
2.60e-05 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 47.58 E-value: 2.60e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 183 ERNVQA-HPDKVAVVSETQ------RWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPL 255
Cdd:PLN02654 95 DRNVEAgNGDKIAIYWEGNepgfdaSLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSV 174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 INTNLRGPSLLHSI------TVAHCSALIYGED--FLEAVTDVAKDLPAN--------LTlfqFNNENNNSETEKNIPQA 319
Cdd:PLN02654 175 VFAGFSAESLAQRIvdckpkVVITCNAVKRGPKtiNLKDIVDAALDESAKngvsvgicLT---YENQLAMKREDTKWQEG 251
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 320 KNLNALLTTASYekPNKTQ---VNHHDKLVYIYTSGTTGLPKAAVISHSRYL-FIAAGIHYTMGFQEEDIFYTplplyhT 395
Cdd:PLN02654 252 RDVWWQDVVPNY--PTKCEvewVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMvYTATTFKYAFDYKPTDVYWC------T 323
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 396 AGGIMCMGQS------VLFGSTVSIrkkFSASNYFADCA-------KYNATIGqYIGEMARYILATKPSEYDQKH----- 457
Cdd:PLN02654 324 ADCGWITGHSyvtygpMLNGATVLV---FEGAPNYPDSGrcwdivdKYKVTIF-YTAPTLVRSLMRDGDEYVTRHsrksl 399
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 458 RVRLVFGNGLRPQIWPQFvqrFNIA-----KVGEFYGATEGNanimnhdntvgaiGFVSRILPKIYPISIIRAD-PDTG- 530
Cdd:PLN02654 400 RVLGSVGEPINPSAWRWF---FNVVgdsrcPISDTWWQTETG-------------GFMITPLPGAWPQKPGSATfPFFGv 463
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 531 EP-IRDRNGL-----CQ--LCAPNE-PGVFigKIVKGNPSREFLGYvdekasakkivkdvFKHGDMAFISGDLLVADEKG 601
Cdd:PLN02654 464 QPvIVDEKGKeiegeCSgyLCVKKSwPGAF--RTLYGDHERYETTY--------------FKPFAGYYFSGDGCSRDKDG 527
|
490 500
....*....|....*....|....*.
gi 939619614 602 YLYFKDRTGDTFRWKGENVSTSEVEA 627
Cdd:PLN02654 528 YYWLTGRVDDVINVSGHRIGTAEVES 553
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
181-517 |
4.75e-05 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 46.88 E-value: 4.75e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 181 VFERNVQAH----PDKVAVVS-ETQRWTFRQVNEHANKVANVLqAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPL 255
Cdd:PRK06814 633 LFEALIEAAkihgFKKLAVEDpVNGPLTYRKLLTGAFVLGRKL-KKNTPPGENVGVMLPNANGAAVTFFALQSAGRVPAM 711
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 256 INTNLRGPSLLHS---------------ITVAHCSALIYGedfLEAVTDVA--KDLPANLTLfqfnnennnseteknipq 318
Cdd:PRK06814 712 INFSAGIANILSAckaaqvktvltsrafIEKARLGPLIEA---LEFGIRIIylEDVRAQIGL------------------ 770
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 319 AKNLNALLTtASYEKPNKTQVNHHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDIFYTPLPLYH---- 394
Cdd:PRK06814 771 ADKIKGLLA-GRFPLVYFCNRDPDDPAVILFTSGSEGTPKGVVLSHRNLLANRAQVAARIDFSPEDKVFNALPVFHsfgl 849
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 395 TAGGIMCMgqsvLFGstVSIRKKFSASNY-------FADcakyNATI----GQYIGEMARYILAtkpseYDQKhRVRLVF 463
Cdd:PRK06814 850 TGGLVLPL----LSG--VKVFLYPSPLHYriipeliYDT----NATIlfgtDTFLNGYARYAHP-----YDFR-SLRYVF 913
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 464 GnGLRP------QIWpqfVQRFNIaKVGEFYGATEG------NANIMNHDNTVGaigfvsRILPKI 517
Cdd:PRK06814 914 A-GAEKvkeetrQTW---MEKFGI-RILEGYGVTETapvialNTPMHNKAGTVG------RLLPGI 968
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
183-252 |
5.74e-05 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 46.48 E-value: 5.74e-05
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 939619614 183 ERNVQAHPDK---VAVVSET---QRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVI 252
Cdd:PRK10524 60 DRHLAKRPEQlalIAVSTETdeeRTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAI 135
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
203-532 |
7.17e-05 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 46.18 E-value: 7.17e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 203 TFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIGVITPLINTNLrgpsllhsitvaHCSALIYGEd 282
Cdd:PRK06060 32 THGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPEL------------HRDDHALAA- 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 283 fleavtdvaKDLPANLTLFQFNNENNNSETEKNIPQAknlnaLLTTASYEKPNKTQVNHHDKLVY-IYTSGTTGLPKAAV 361
Cdd:PRK06060 99 ---------RNTEPALVVTSDALRDRFQPSRVAEAAE-----LMSEAARVAPGGYEPMGGDALAYaTYTSGTTGPPKAAI 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 362 ISHSR-YLFIAAGIHYTMGFQEEDIFYTPLPLYHTAGgimcMGQSVLF-----GSTVSIRKKFSASNYFADCAKYNATIG 435
Cdd:PRK06060 165 HRHADpLTFVDAMCRKALRLTPEDTGLCSARMYFAYG----LGNSVWFplatgGSAVINSAPVTPEAAAILSARFGPSVL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 436 QYIGE-MARYILATKPSEYdQKHRVRLVFGNGLRPQIWPQFVQRFNIAKVGEFYGATE-GNANIMN--HDNTVGAIGfvs 511
Cdd:PRK06060 241 YGVPNfFARVIDSCSPDSF-RSLRCVVSAGEALELGLAERLMEFFGGIPILDGIGSTEvGQTFVSNrvDEWRLGTLG--- 316
|
330 340
....*....|....*....|.
gi 939619614 512 RILPKiYPISIIRADPDTGEP 532
Cdd:PRK06060 317 RVLPP-YEIRVVAPDGTTAGP 336
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
348-428 |
1.41e-04 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 44.98 E-value: 1.41e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 348 IYTSGTTGLPKAAVISHSRylfIAAGIHY---TMGFQEEDIFYTPLPLYHTAGGIMCMGQSVLFGSTVSIRKKFSASNYF 424
Cdd:PRK07787 134 VYTSGTTGPPKGVVLSRRA---IAADLDAlaeAWQWTADDVLVHGLPLFHVHGLVLGVLGPLRIGNRFVHTGRPTPEAYA 210
|
....
gi 939619614 425 ADCA 428
Cdd:PRK07787 211 QALS 214
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
189-254 |
2.36e-04 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 44.43 E-value: 2.36e-04
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 939619614 189 HPDKVAVV-------SETQRWTFRQVNEHANKVANVLQAQGYKKGDVVALLLENRAEYVATWLGLSKIG----VITP 254
Cdd:cd17647 1 FPERTCVVetpslnsSKTRSFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGatfsVIDP 77
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
201-394 |
3.56e-04 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 43.74 E-value: 3.56e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 201 RW-TFRQVNEHANKVANVLQAQGYK--KGDVVALLLENRAEYVATWLGLSKIG-VITPLINTnlRGPSLLHSItVAHC-- 274
Cdd:cd05927 4 EWiSYKEVAERADNIGSALRSLGGKpaPASFVGIYSINRPEWIISELACYAYSlVTVPLYDT--LGPEAIEYI-LNHAei 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 275 SALIYGEDF----LEAVTDVAKDLPANLTLfqfnnennnsetekniPQAKNLNallttasyekpnktqvnhhdklVYIYT 350
Cdd:cd05927 81 SIVFCDAGVkvysLEEFEKLGKKNKVPPPP----------------PKPEDLA----------------------TICYT 122
|
170 180 190 200
....*....|....*....|....*....|....*....|....*...
gi 939619614 351 SGTTGLPKAAVISHSRYLFIAAGIHYTMG----FQEEDIFYTPLPLYH 394
Cdd:cd05927 123 SGTTGNPKGVMLTHGNIVSNVAGVFKILEilnkINPTDVYISYLPLAH 170
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
552-683 |
1.22e-03 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 41.90 E-value: 1.22e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939619614 552 IGKIVKGNPSReFLGYVDEKASAKKIvkdvfkhgdmaFISGDLLVADEKGYLYFKDRTGDTFRWKGENVSTSEVEAQVSN 631
Cdd:PRK07445 301 TGNITIQAQSL-ALGYYPQILDSQGI-----------FETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILA 368
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|...
gi 939619614 632 VAGYKDTVVYGVTIPHTeGRAgMAAIYDPER-ELDLDVFAASLAKVLPAYARP 683
Cdd:PRK07445 369 TGLVQDVCVLGLPDPHW-GEV-VTAIYVPKDpSISLEELKTAIKDQLSPFKQP 419
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
331-394 |
1.24e-03 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 42.32 E-value: 1.24e-03
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 939619614 331 YEKPNKTQvnhHDKLVYIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMG-----FQEEDIFYTPLPLYH 394
Cdd:PLN02614 215 YDLPIKKK---SDICTIMYTSGTTGDPKGVMISNESIVTLIAGVIRLLKsanaaLTVKDVYLSYLPLAH 280
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
347-414 |
3.63e-03 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 40.63 E-value: 3.63e-03
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 939619614 347 YIYTSGTTGLPKAAVISHSRYLFIAAGIHYTMGFQEEDifytP------LPLYHTAGGIMCMGQSVLFGSTVSI 414
Cdd:PRK08180 214 FLFTSGSTGLPKAVINTHRMLCANQQMLAQTFPFLAEE----PpvlvdwLPWNHTFGGNHNLGIVLYNGGTLYI 283
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