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Conserved domains on  [gi|384871683|ref|NP_001245203|]
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DNA mismatch repair protein Mlh1 isoform 3 [Homo sapiens]

Protein Classification

MutL_Trans_MLH1 and Mlh1_C domain-containing protein( domain architecture ID 12932958)

protein containing domains MutL_Trans_MLH1, mutL, and Mlh1_C

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mlh1_C pfam16413
DNA mismatch repair protein Mlh1 C-terminus; This is the C-terminal domain of DNA mismatch ...
 261-515 1.20e-134

DNA mismatch repair protein Mlh1 C-terminus; This is the C-terminal domain of DNA mismatch repair protein Mlh1, these proteins belong to the MutL family. This domain forms part of the endonuclease active site.


:

Pssm-ID: 465109  Cd Length: 260  Bit Score: 390.36  E-value: 1.20e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  261 NLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQ--WALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEP 338
Cdd:pfam16413   1 RLTSIKELRAEVEESMHKELTEIFANHTFVGCVDERrrLALIQHGTKLYLVDYGALSEELFYQIGLTDFGNFGRIRLSPP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  339 APLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILR 418
Cdd:pfam16413  81 LSLYELLELALESEESGWTEEDGPKEELAEKIVELLIEKAEMLEEYFSIEIDEDGNLESLPLLLKGYTPNLDKLPLFLLR 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  419 LATEVNWDEEKECFESLSKECAMFYSIRKQY----ISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFT 494
Cdd:pfam16413 161 LATEVDWDDEKECFETFLRELALFYSPEPLPppsnDESNDDEDEEEDEEIEARREEWKWVIEHVLFPAIKRRLLPPKSLL 240

                         250       260
                  ....*....|....*....|.
gi 384871683  495 EDGnILQLANLPDLYKVFERC 515
Cdd:pfam16413 241 KDT-VVQVANLPDLYKVFERC 260
MutL_Trans_MLH1 cd03483
MutL_Trans_MLH1: transducer domain, having a ribosomal S5 domain 2-like fold, found in ...
 1-94 8.15e-60

MutL_Trans_MLH1: transducer domain, having a ribosomal S5 domain 2-like fold, found in proteins similar to yeast and human MLH1 (MutL homologue 1). This transducer domain is homologous to the second domain of the DNA gyrase B subunit, which is known to be important in nucleotide hydrolysis and the transduction of structural signals from ATP-binding site to the DNA breakage/reunion regions of the enzymes. MLH1 forms heterodimers with PMS2, PMS1 and MLH3. These three complexes have distinct functions in meiosis. hMLH1-hPMS2 also participates in the repair of all DNA mismatch repair (MMR) substrates. Roles for hMLH1-hPMS1 or hMLH1-hMLH3 in MMR have not been established. Cells lacking hMLH1 have a strong mutator phenotype and display microsatellite instability (MSI). Mutation in hMLH1 causes predisposition to HNPCC, Muir-Torre syndrome and Turcot syndrome (HNPCC variant). Mutation in hMLH1 accounts for a large fraction of HNPCC families.


:

Pssm-ID: 239565 [Multi-domain]  Cd Length: 127  Bit Score: 193.22  E-value: 8.15e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683   1 MNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEES 80
Cdd:cd03483   34 VKGLISNANYSKKKIIFILFINNRLVECSALRRAIENVYANYLPKGAHPFVYLSLEIPPENVDVNVHPTKREVHFLNEEE 113

                         90
                 ....*....|....
gi 384871683  81 ILERVQQHIESKLL 94
Cdd:cd03483  114 IIERIQKLVEDKLS 127
mutL super family cl35064
DNA mismatch repair endonuclease MutL;
19-186 8.81e-19

DNA mismatch repair endonuclease MutL;


The actual alignment was detected with superfamily member PRK00095:

Pssm-ID: 234630 [Multi-domain]  Cd Length: 617  Bit Score: 89.51  E-value: 8.81e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  19 LFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSN- 97
Cdd:PRK00095 251 LFVNGRYVRDKLLNHAIRQAYHDLLPRGRYPAFVLFLELDPHQVDVNVHPAKHEVRFRDERLVHDLIVQAIQEALAQSGl 330

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  98 ---------------SSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSK- 161
Cdd:PRK00095 331 ipaaaganqvlepaePEPLPLQQTPLYASGSSPPASSPSSAPPEQSEESQEESSAEKNPLQPNASQSEAAAAASAEAAAa 410

                        170       180
                 ....*....|....*....|....*.
gi 384871683 162 -PLSSQPQAIVTEDKTDISSGRARQQ 186
Cdd:PRK00095 411 aPAAAPEPAEAAEEADSFPLGYALGQ 436
 
Name Accession Description Interval E-value
Mlh1_C pfam16413
DNA mismatch repair protein Mlh1 C-terminus; This is the C-terminal domain of DNA mismatch ...
 261-515 1.20e-134

DNA mismatch repair protein Mlh1 C-terminus; This is the C-terminal domain of DNA mismatch repair protein Mlh1, these proteins belong to the MutL family. This domain forms part of the endonuclease active site.


Pssm-ID: 465109  Cd Length: 260  Bit Score: 390.36  E-value: 1.20e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  261 NLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQ--WALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEP 338
Cdd:pfam16413   1 RLTSIKELRAEVEESMHKELTEIFANHTFVGCVDERrrLALIQHGTKLYLVDYGALSEELFYQIGLTDFGNFGRIRLSPP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  339 APLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILR 418
Cdd:pfam16413  81 LSLYELLELALESEESGWTEEDGPKEELAEKIVELLIEKAEMLEEYFSIEIDEDGNLESLPLLLKGYTPNLDKLPLFLLR 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  419 LATEVNWDEEKECFESLSKECAMFYSIRKQY----ISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFT 494
Cdd:pfam16413 161 LATEVDWDDEKECFETFLRELALFYSPEPLPppsnDESNDDEDEEEDEEIEARREEWKWVIEHVLFPAIKRRLLPPKSLL 240

                         250       260
                  ....*....|....*....|.
gi 384871683  495 EDGnILQLANLPDLYKVFERC 515
Cdd:pfam16413 241 KDT-VVQVANLPDLYKVFERC 260
MutL_Trans_MLH1 cd03483
MutL_Trans_MLH1: transducer domain, having a ribosomal S5 domain 2-like fold, found in ...
 1-94 8.15e-60

MutL_Trans_MLH1: transducer domain, having a ribosomal S5 domain 2-like fold, found in proteins similar to yeast and human MLH1 (MutL homologue 1). This transducer domain is homologous to the second domain of the DNA gyrase B subunit, which is known to be important in nucleotide hydrolysis and the transduction of structural signals from ATP-binding site to the DNA breakage/reunion regions of the enzymes. MLH1 forms heterodimers with PMS2, PMS1 and MLH3. These three complexes have distinct functions in meiosis. hMLH1-hPMS2 also participates in the repair of all DNA mismatch repair (MMR) substrates. Roles for hMLH1-hPMS1 or hMLH1-hMLH3 in MMR have not been established. Cells lacking hMLH1 have a strong mutator phenotype and display microsatellite instability (MSI). Mutation in hMLH1 causes predisposition to HNPCC, Muir-Torre syndrome and Turcot syndrome (HNPCC variant). Mutation in hMLH1 accounts for a large fraction of HNPCC families.


Pssm-ID: 239565 [Multi-domain]  Cd Length: 127  Bit Score: 193.22  E-value: 8.15e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683   1 MNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEES 80
Cdd:cd03483   34 VKGLISNANYSKKKIIFILFINNRLVECSALRRAIENVYANYLPKGAHPFVYLSLEIPPENVDVNVHPTKREVHFLNEEE 113

                         90
                 ....*....|....
gi 384871683  81 ILERVQQHIESKLL 94
Cdd:cd03483  114 IIERIQKLVEDKLS 127
DNA_mis_repair pfam01119
DNA mismatch repair protein, C-terminal domain; This family represents the C-terminal domain ...
 1-93 9.60e-35

DNA mismatch repair protein, C-terminal domain; This family represents the C-terminal domain of the mutL/hexB/PMS1 family. This domain has a ribosomal S5 domain 2-like fold.


Pssm-ID: 426060 [Multi-domain]  Cd Length: 117  Bit Score: 126.46  E-value: 9.60e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683    1 MNGYISNANYS-VKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEE 79
Cdd:pfam01119  24 LSGYISKPTLSrSNRDYQYLFVNGRPVRDKLLSHAIREAYRDLLPKGRYPVAVLFLEIDPELVDVNVHPTKREVRFRDER 103

                          90
                  ....*....|....
gi 384871683   80 SILERVQQHIESKL 93
Cdd:pfam01119 104 EVYDFIKEALREAL 117
mutl TIGR00585
DNA mismatch repair protein MutL; All proteins in this family for which the functions are ...
 2-74 1.30e-22

DNA mismatch repair protein MutL; All proteins in this family for which the functions are known are involved in the process of generalized mismatch repair. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273155 [Multi-domain]  Cd Length: 312  Bit Score: 98.48  E-value: 1.30e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 384871683    2 NGYISNANYSVKKCI--FLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVH 74
Cdd:TIGR00585 238 EGFISQPNVTRSRRSgwQFLFINGRPVELKLLLKAIREVYHEYLPKGQYPVFVLNLEIDPELVDVNVHPDKKEVR 312
MutL COG0323
DNA mismatch repair ATPase MutL [Replication, recombination and repair];
3-96 9.52e-21

DNA mismatch repair ATPase MutL [Replication, recombination and repair];


Pssm-ID: 440092 [Multi-domain]  Cd Length: 515  Bit Score: 95.11  E-value: 9.52e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683   3 GYISNANY--SVKKCIFLlFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEES 80
Cdd:COG0323  235 GYIGKPEFsrSNRDYQYF-FVNGRPVRDKLLSHAVREAYRDLLPKGRYPVAVLFLELDPELVDVNVHPTKTEVRFRDERE 313

                         90
                 ....*....|....*.
gi 384871683  81 ILERVQQHIESKLLGS 96
Cdd:COG0323  314 VYDLVRSAVREALAQA 329
mutL PRK00095
DNA mismatch repair endonuclease MutL;
19-186 8.81e-19

DNA mismatch repair endonuclease MutL;


Pssm-ID: 234630 [Multi-domain]  Cd Length: 617  Bit Score: 89.51  E-value: 8.81e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  19 LFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSN- 97
Cdd:PRK00095 251 LFVNGRYVRDKLLNHAIRQAYHDLLPRGRYPAFVLFLELDPHQVDVNVHPAKHEVRFRDERLVHDLIVQAIQEALAQSGl 330

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  98 ---------------SSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSK- 161
Cdd:PRK00095 331 ipaaaganqvlepaePEPLPLQQTPLYASGSSPPASSPSSAPPEQSEESQEESSAEKNPLQPNASQSEAAAAASAEAAAa 410

                        170       180
                 ....*....|....*....|....*.
gi 384871683 162 -PLSSQPQAIVTEDKTDISSGRARQQ 186
Cdd:PRK00095 411 aPAAAPEPAEAAEEADSFPLGYALGQ 436
 
Name Accession Description Interval E-value
Mlh1_C pfam16413
DNA mismatch repair protein Mlh1 C-terminus; This is the C-terminal domain of DNA mismatch ...
 261-515 1.20e-134

DNA mismatch repair protein Mlh1 C-terminus; This is the C-terminal domain of DNA mismatch repair protein Mlh1, these proteins belong to the MutL family. This domain forms part of the endonuclease active site.


Pssm-ID: 465109  Cd Length: 260  Bit Score: 390.36  E-value: 1.20e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  261 NLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQ--WALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEP 338
Cdd:pfam16413   1 RLTSIKELRAEVEESMHKELTEIFANHTFVGCVDERrrLALIQHGTKLYLVDYGALSEELFYQIGLTDFGNFGRIRLSPP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  339 APLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILR 418
Cdd:pfam16413  81 LSLYELLELALESEESGWTEEDGPKEELAEKIVELLIEKAEMLEEYFSIEIDEDGNLESLPLLLKGYTPNLDKLPLFLLR 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  419 LATEVNWDEEKECFESLSKECAMFYSIRKQY----ISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFT 494
Cdd:pfam16413 161 LATEVDWDDEKECFETFLRELALFYSPEPLPppsnDESNDDEDEEEDEEIEARREEWKWVIEHVLFPAIKRRLLPPKSLL 240

                         250       260
                  ....*....|....*....|.
gi 384871683  495 EDGnILQLANLPDLYKVFERC 515
Cdd:pfam16413 241 KDT-VVQVANLPDLYKVFERC 260
MutL_Trans_MLH1 cd03483
MutL_Trans_MLH1: transducer domain, having a ribosomal S5 domain 2-like fold, found in ...
 1-94 8.15e-60

MutL_Trans_MLH1: transducer domain, having a ribosomal S5 domain 2-like fold, found in proteins similar to yeast and human MLH1 (MutL homologue 1). This transducer domain is homologous to the second domain of the DNA gyrase B subunit, which is known to be important in nucleotide hydrolysis and the transduction of structural signals from ATP-binding site to the DNA breakage/reunion regions of the enzymes. MLH1 forms heterodimers with PMS2, PMS1 and MLH3. These three complexes have distinct functions in meiosis. hMLH1-hPMS2 also participates in the repair of all DNA mismatch repair (MMR) substrates. Roles for hMLH1-hPMS1 or hMLH1-hMLH3 in MMR have not been established. Cells lacking hMLH1 have a strong mutator phenotype and display microsatellite instability (MSI). Mutation in hMLH1 causes predisposition to HNPCC, Muir-Torre syndrome and Turcot syndrome (HNPCC variant). Mutation in hMLH1 accounts for a large fraction of HNPCC families.


Pssm-ID: 239565 [Multi-domain]  Cd Length: 127  Bit Score: 193.22  E-value: 8.15e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683   1 MNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEES 80
Cdd:cd03483   34 VKGLISNANYSKKKIIFILFINNRLVECSALRRAIENVYANYLPKGAHPFVYLSLEIPPENVDVNVHPTKREVHFLNEEE 113

                         90
                 ....*....|....
gi 384871683  81 ILERVQQHIESKLL 94
Cdd:cd03483  114 IIERIQKLVEDKLS 127
MutL_Trans cd00782
MutL_Trans: transducer domain, having a ribosomal S5 domain 2-like fold, conserved in the ...
 1-93 1.25e-36

MutL_Trans: transducer domain, having a ribosomal S5 domain 2-like fold, conserved in the C-terminal domain of DNA mismatch repair (MutL/MLH1/PMS2) family. This transducer domain is homologous to the second domain of the DNA gyrase B subunit, which is known to be important in nucleotide hydrolysis and the transduction of structural signals from ATP-binding site to the DNA breakage/reunion regions of the enzymes. Included in this group are proteins similar to human MLH1, hPMS2, hPMS1, hMLH3 and E. coli MutL, MLH1 forms heterodimers with PMS2, PMS1 and MLH3. These three complexes have distinct functions in meiosis. hMLH1-hPMS2 also participates in the repair of all DNA mismatch repair (MMR) substrates. Roles for hMLH1-hPMS1 or hMLH1-hMLH3 in MMR have not been established. Cells lacking either hMLH1 or hPMS2 have a strong mutator phenotype and display microsatellite instability (MSI). Mutation in hMLH1 causes predisposition to HNPCC, Muir-Torre syndrome and Turcot syndrome (HNPCC variant). Mutation in hPMS2 causes predisposition to HPNCC and Turcot syndrome. Mutation in hMLH1 accounts for a large fraction of HNPCC families. There is no convincing evidence to support hPMS1 having a role in HNPCC predisposition. It has been suggested that hMLH3 may be a low risk gene for colorectal cancer; however there is little evidence to support it having a role in classical HNPCC. It has been suggested that during initiation of DNA mismatch repair in E. coli, the mismatch recognition protein MutS recruits MutL in the presence of ATP. The MutS(ATP)-MutL ternary complex formed, then recruits the latent endonuclease MutH.


Pssm-ID: 238405 [Multi-domain]  Cd Length: 122  Bit Score: 131.51  E-value: 1.25e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683   1 MNGYISNANYSV-KKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEE 79
Cdd:cd00782   29 ISGYISKPDFGRsSKDRQFLFVNGRPVRDKLLSKAINEAYRSYLPKGRYPVFVLNLELPPELVDVNVHPTKREVRFSDEE 108

                         90
                 ....*....|....
gi 384871683  80 SILERVQQHIESKL 93
Cdd:cd00782  109 EVLELIREALRSAL 122
DNA_mis_repair pfam01119
DNA mismatch repair protein, C-terminal domain; This family represents the C-terminal domain ...
 1-93 9.60e-35

DNA mismatch repair protein, C-terminal domain; This family represents the C-terminal domain of the mutL/hexB/PMS1 family. This domain has a ribosomal S5 domain 2-like fold.


Pssm-ID: 426060 [Multi-domain]  Cd Length: 117  Bit Score: 126.46  E-value: 9.60e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683    1 MNGYISNANYS-VKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEE 79
Cdd:pfam01119  24 LSGYISKPTLSrSNRDYQYLFVNGRPVRDKLLSHAIREAYRDLLPKGRYPVAVLFLEIDPELVDVNVHPTKREVRFRDER 103

                          90
                  ....*....|....
gi 384871683   80 SILERVQQHIESKL 93
Cdd:pfam01119 104 EVYDFIKEALREAL 117
mutl TIGR00585
DNA mismatch repair protein MutL; All proteins in this family for which the functions are ...
 2-74 1.30e-22

DNA mismatch repair protein MutL; All proteins in this family for which the functions are known are involved in the process of generalized mismatch repair. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273155 [Multi-domain]  Cd Length: 312  Bit Score: 98.48  E-value: 1.30e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 384871683    2 NGYISNANYSVKKCI--FLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVH 74
Cdd:TIGR00585 238 EGFISQPNVTRSRRSgwQFLFINGRPVELKLLLKAIREVYHEYLPKGQYPVFVLNLEIDPELVDVNVHPDKKEVR 312
MutL COG0323
DNA mismatch repair ATPase MutL [Replication, recombination and repair];
3-96 9.52e-21

DNA mismatch repair ATPase MutL [Replication, recombination and repair];


Pssm-ID: 440092 [Multi-domain]  Cd Length: 515  Bit Score: 95.11  E-value: 9.52e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683   3 GYISNANY--SVKKCIFLlFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEES 80
Cdd:COG0323  235 GYIGKPEFsrSNRDYQYF-FVNGRPVRDKLLSHAVREAYRDLLPKGRYPVAVLFLELDPELVDVNVHPTKTEVRFRDERE 313

                         90
                 ....*....|....*.
gi 384871683  81 ILERVQQHIESKLLGS 96
Cdd:COG0323  314 VYDLVRSAVREALAQA 329
mutL PRK00095
DNA mismatch repair endonuclease MutL;
19-186 8.81e-19

DNA mismatch repair endonuclease MutL;


Pssm-ID: 234630 [Multi-domain]  Cd Length: 617  Bit Score: 89.51  E-value: 8.81e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  19 LFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSN- 97
Cdd:PRK00095 251 LFVNGRYVRDKLLNHAIRQAYHDLLPRGRYPAFVLFLELDPHQVDVNVHPAKHEVRFRDERLVHDLIVQAIQEALAQSGl 330

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683  98 ---------------SSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSK- 161
Cdd:PRK00095 331 ipaaaganqvlepaePEPLPLQQTPLYASGSSPPASSPSSAPPEQSEESQEESSAEKNPLQPNASQSEAAAAASAEAAAa 410

                        170       180
                 ....*....|....*....|....*.
gi 384871683 162 -PLSSQPQAIVTEDKTDISSGRARQQ 186
Cdd:PRK00095 411 aPAAAPEPAEAAEEADSFPLGYALGQ 436
TopoII_MutL_Trans cd00329
MutL_Trans: transducer domain, having a ribosomal S5 domain 2-like fold, conserved in the ...
 3-74 1.03e-16

MutL_Trans: transducer domain, having a ribosomal S5 domain 2-like fold, conserved in the C-terminal domain of type II DNA topoisomerases (Topo II) and DNA mismatch repair (MutL/MLH1/PMS2) proteins. This transducer domain is homologous to the second domain of the DNA gyrase B subunit, which is known to be important in nucleotide hydrolysis and the transduction of structural signals from ATP-binding site to the DNA breakage/reunion regions of the enzymes. The GyrB dimerizes in response to ATP binding, and is homologous to the N-terminal half of eukaryotic Topo II and the ATPase fragment of MutL. Type II DNA topoisomerases catalyze the ATP-dependent transport of one DNA duplex through another, in the process generating transient double strand breaks via covalent attachments to both DNA strands at the 5' positions. Included in this group are proteins similar to human MLH1 and PMS2. MLH1 forms a heterodimer with PMS2 which functions in meiosis and in DNA mismatch repair (MMR). Cells lacking either hMLH1 or hPMS2 have a strong mutator phenotype and display microsatellite instability (MSI). Mutation in hMLH1 accounts for a large fraction of Lynch syndrome (HNPCC) families.


Pssm-ID: 238202 [Multi-domain]  Cd Length: 107  Bit Score: 75.76  E-value: 1.03e-16
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 384871683   3 GYISNANYSV-KKCIFLLFINHRLV-ESTSLRKAIETVYAAYL---PKNTHPFLYLSLEISPQNVDVNVHPTKHEVH 74
Cdd:cd00329   31 GAISYPDSGRsSKDRQFSFVNGRPVrEGGTHVKAVREAYTRALngdDVRRYPVAVLSLKIPPSLVDVNVHPTKEEVR 107
MutL_Trans_MutL cd03482
MutL_Trans_MutL: transducer domain, having a ribosomal S5 domain 2-like fold, found in ...
 19-93 6.40e-13

MutL_Trans_MutL: transducer domain, having a ribosomal S5 domain 2-like fold, found in proteins similar to Escherichia coli MutL. EcMutL belongs to the DNA mismatch repair (MutL/MLH1/PMS2) family. This transducer domain is homologous to the second domain of the DNA gyrase B subunit, which is known to be important in nucleotide hydrolysis and the transduction of structural signals from the ATP-binding site to the DNA breakage/reunion regions of the enzymes. It has been suggested that during initiation of DNA mismatch repair in E. coli, the mismatch recognition protein MutS recruits MutL in the presence of ATP. The MutS(ATP)-MutL ternary complex formed, then recruits the latent endonuclease MutH. Prokaryotic MutS and MutL are homodimers.


Pssm-ID: 239564 [Multi-domain]  Cd Length: 123  Bit Score: 65.30  E-value: 6.40e-13
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 384871683  19 LFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKL 93
Cdd:cd03482   48 FYVNGRMVRDKLISHAVRQAYSDVLHGGRHPAYVLYLELDPAQVDVNVHPAKHEVRFRDSRLVHDFIYHAVKKAL 122
MutL_Trans_hPMS_2_like cd03484
MutL_Trans_hPMS2_like: transducer domain, having a ribosomal S5 domain 2-like fold, found in ...
 3-91 2.44e-09

MutL_Trans_hPMS2_like: transducer domain, having a ribosomal S5 domain 2-like fold, found in proteins similar to human PSM2 (hPSM2). hPSM2 belongs to the DNA mismatch repair (MutL/MLH1/PMS2) family. This transducer domain is homologous to the second domain of the DNA gyrase B subunit, which is known to be important in nucleotide hydrolysis and the transduction of structural signals from ATP-binding site to the DNA breakage/reunion regions of the enzymes. Included in this group are proteins similar to yeast PMS1. The yeast MLH1-PMS1 and the human MLH1-PMS2 heterodimers play a role in meiosis. hMLH1-hPMS2 also participates in the repair of all DNA mismatch repair (MMR) substrates. Cells lacking hPMS2 have a strong mutator phenotype and display microsatellite instability (MSI). Mutation in hPMS2 causes predisposition to HPNCC and Turcot syndrome.


Pssm-ID: 239566 [Multi-domain]  Cd Length: 142  Bit Score: 55.74  E-value: 2.44e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 384871683   3 GYISnanysvkKCIF----------LLFINHRLVESTSLRKAIETVYAAYlPKNTHPFLYLSLEISPQNVDVNVHPTKHE 72
Cdd:cd03484   49 GYIS-------KPSHgcgrsssdrqFFYINGRPVDLKKVAKLINEVYKSF-NSRQYPFFILNISLPTSLYDVNVTPDKRT 120

                         90
                 ....*....|....*....
gi 384871683  73 VHFLHEESILERVQQHIES 91
Cdd:cd03484  121 VLLHDEDRLIDTLKTSLSE 139
MutL_Trans_hPMS_1_like cd03485
MutL_Trans_hPMS1_like: transducer domain, having a ribosomal S5 domain 2-like fold, found in ...
 19-91 9.71e-08

MutL_Trans_hPMS1_like: transducer domain, having a ribosomal S5 domain 2-like fold, found in proteins similar to human PSM1 (hPSM1) and yeast MLH2. hPSM1 and yMLH2 are members of the DNA mismatch repair (MutL/MLH1/PMS2) family. This transducer domain is homologous to the second domain of the DNA gyrase B subunit, which is known to be important in nucleotide hydrolysis and the transduction of structural signals from ATP-binding site to the DNA breakage/reunion regions of the enzymes. PMS1 forms a heterodimer with MLH1. The MLH1-PMS1 complex functions in meiosis. Loss of yMLH2 results in a small but significant decrease in spore viability and a significant increase in gene conversion frequencies. A role for hMLH1-hPMS1 in DNA mismatch repair has not been established. Mutation in hMLH1 accounts for a large fraction of Lynch syndrome (HNPCC) families, however there is no convincing evidence to support hPMS1 having a role in HNPCC predisposition.


Pssm-ID: 239567 [Multi-domain]  Cd Length: 132  Bit Score: 50.73  E-value: 9.71e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 384871683  19 LFINHRLVESTS-LRKAIETVYAAYLPKNT---HPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIES 91
Cdd:cd03485   53 ISVNSRPVSLGKdIGKLLRQYYSSAYRKSSlrrYPVFFLNILCPPGLVDVNIEPDKDDVLLQNKEAVLQAVENLLES 129
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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