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Conserved domains on  [gi|1573307390|ref|NP_001355564|]
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deoxyribonuclease TATDN1 isoform 6 [Mus musculus]

Protein Classification

amidohydrolase family protein( domain architecture ID 330)

metal-dependent amidohydrolase family protein having a conserved metal binding site, usually involving four histidines and one aspartic acid residue

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
metallo-dependent_hydrolases super family cl00281
Superfamily of metallo-dependent hydrolases (also called amidohydrolase superfamily) is a ...
1-179 1.83e-37

Superfamily of metallo-dependent hydrolases (also called amidohydrolase superfamily) is a large group of proteins that show conservation in their 3-dimensional fold (TIM barrel) and in details of their active site. The vast majority of the members have a conserved metal binding site, involving four histidines and one aspartic acid residue. In the common reaction mechanism, the metal ion (or ions) deprotonate a water molecule for a nucleophilic attack on the substrate. The family includes urease alpha, adenosine deaminase, phosphotriesterase dihydroorotases, allantoinases, hydantoinases, AMP-, adenine and cytosine deaminases, imidazolonepropionase, aryldialkylphosphatase, chlorohydrolases, formylmethanofuran dehydrogenases and others.


The actual alignment was detected with superfamily member cd01310:

Pssm-ID: 444807 [Multi-domain]  Cd Length: 251  Bit Score: 129.23  E-value: 1.83e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEFEkgspDQYLAGLLSLAENNKgkVVAIGECGLDFDRLQFcPKDT 80
Cdd:cd01310    33 IVVGTDLKSSKRALELAKKYDNVYAAVGLHPHDADEHV----DEDLDLLELLAANPK--VVAIGEIGLDYYRDKS-PREV 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  81 QL-------------------------------------------------------------------NSLKTEAN--L 91
Cdd:cd01310   106 QKevfraqlelakelnlpvvihsrdahedvleilkeygppkrgvfhcfsgsaeeakelldlgfyisisgIVTFKNANelR 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 EVLKSIPSEKLMIETDAPWCGVKSTHagskyintsfptkkkwenghclKDRNEPCHIIQILEIMSAVREEDPLELANTLY 171
Cdd:cd01310   186 EVVKEIPLERLLLETDSPYLAPVPFR----------------------GKRNEPAYVKHVAEKIAELKGISVEEVAEVTT 243

                  ....*...
gi 1573307390 172 NNTIKVFF 179
Cdd:cd01310   244 ENAKRLFG 251
 
Name Accession Description Interval E-value
TatD_DNAse cd01310
TatD like proteins; E.coli TatD is a cytoplasmic protein, shown to have magnesium dependent ...
1-179 1.83e-37

TatD like proteins; E.coli TatD is a cytoplasmic protein, shown to have magnesium dependent DNase activity.


Pssm-ID: 238635 [Multi-domain]  Cd Length: 251  Bit Score: 129.23  E-value: 1.83e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEFEkgspDQYLAGLLSLAENNKgkVVAIGECGLDFDRLQFcPKDT 80
Cdd:cd01310    33 IVVGTDLKSSKRALELAKKYDNVYAAVGLHPHDADEHV----DEDLDLLELLAANPK--VVAIGEIGLDYYRDKS-PREV 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  81 QL-------------------------------------------------------------------NSLKTEAN--L 91
Cdd:cd01310   106 QKevfraqlelakelnlpvvihsrdahedvleilkeygppkrgvfhcfsgsaeeakelldlgfyisisgIVTFKNANelR 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 EVLKSIPSEKLMIETDAPWCGVKSTHagskyintsfptkkkwenghclKDRNEPCHIIQILEIMSAVREEDPLELANTLY 171
Cdd:cd01310   186 EVVKEIPLERLLLETDSPYLAPVPFR----------------------GKRNEPAYVKHVAEKIAELKGISVEEVAEVTT 243

                  ....*...
gi 1573307390 172 NNTIKVFF 179
Cdd:cd01310   244 ENAKRLFG 251
TatD COG0084
Tat protein secretion system quality control protein TatD (DNase activity) [Cell motility];
1-178 7.50e-28

Tat protein secretion system quality control protein TatD (DNase activity) [Cell motility];


Pssm-ID: 223162 [Multi-domain]  Cd Length: 256  Bit Score: 104.60  E-value: 7.50e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEfekgSPDQYLAGLLSLAENNKgKVVAIGECGLDFDRLQFCPKDT 80
Cdd:COG0084    35 VVVGTDLEDFKRALELAEKYPNVYAAVGVHPLDADE----HSEEDLEELEQLAEHHP-KVVAIGEIGLDYYWDKEPDKER 109
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  81 Q-----------------------------LNSLKTE----------------------------------------ANL 91
Cdd:COG0084   110 QeevfeaqlelakelnlpviihtrdahedtLEILKEEgapvggvlhcfsgsaeearklldlgfyisisgivtfknaeKLR 189
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 EVLKSIPSEKLMIETDAPWCgvksthagskyinTSFPTKKKwenghclkdRNEPCHIIQILEIMSAVREEDPLELANTLY 171
Cdd:COG0084   190 EVARELPLDRLLLETDAPYL-------------APVPYRGK---------RNEPAYVRHVAEKLAELKGISAEEVAEITT 247

                  ....*..
gi 1573307390 172 NNTIKVF 178
Cdd:COG0084   248 ENAKRLF 254
TatD_DNase pfam01026
TatD related DNase; This family of proteins are related to a large superfamily of ...
1-178 1.09e-25

TatD related DNase; This family of proteins are related to a large superfamily of metalloenzymes. TatD, a member of this family has been shown experimentally to be a DNase enzyme.


Pssm-ID: 425997 [Multi-domain]  Cd Length: 253  Bit Score: 98.87  E-value: 1.09e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMF-FSTVGCHPTRCDEFEkgspDQYLAGLLSLAENnkGKVVAIGECGLDFDRLQFCPKD 79
Cdd:pfam01026  32 VVVGTDLEDFLRVLELAEKYPDRvYAAVGVHPHEADEAS----EDDLEALEKLAEH--PKVVAIGEIGLDYYYVDESPKE 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  80 TQ-----------------------------LNSLKTEANL--------------------------------------- 91
Cdd:pfam01026 106 AQeevfrrqlelakelglpvvihtrdaeedlLEILKEAGAPgargvlhcftgsveearkfldlgfyisisgivtfknakk 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 --EVLKSIPSEKLMIETDAPWCgvksthagskyinTSFPTKKKwenghclkdRNEPCHIIQILEIMSAVREEDPLELANT 169
Cdd:pfam01026 186 lrEVAAAIPLDRLLVETDAPYL-------------APVPYRGK---------RNEPAYVPYVVEKLAELKGISPEEVAEI 243

                  ....*....
gi 1573307390 170 LYNNTIKVF 178
Cdd:pfam01026 244 TTENAERLF 252
PRK10425 PRK10425
3'-5' ssDNA/RNA exonuclease TatD;
1-178 8.25e-15

3'-5' ssDNA/RNA exonuclease TatD;


Pssm-ID: 182449  Cd Length: 258  Bit Score: 70.08  E-value: 8.25e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEFEKGSPDQylagLLSLAEnnKGKVVAIGECGLDFDRlQFCPKDT 80
Cdd:PRK10425   33 LITGTNLRESQQAQKLARQYPSCWSTAGVHPHDSSQWQAATEEA----IIELAA--QPEVVAIGECGLDFNR-NFSTPEE 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  81 Q------------------------------------LNSLK---------TEANL------------------------ 91
Cdd:PRK10425  106 QerafvaqlaiaaelnmpvfmhcrdaherfmallepwLDKLPgavlhcftgTREEMqaclarglyigitgwvcderrgle 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 --EVLKSIPSEKLMIETDAPWCGVKSTHAGskyintsfPTKKkwenghclkdRNEPCHIIQILEIMSAVREEDPLELANT 169
Cdd:PRK10425  186 lrELLPLIPAERLLLETDAPYLLPRDLTPK--------PASR----------RNEPAFLPHILQRIAHWRGEDAAWLAAT 247

                  ....*....
gi 1573307390 170 LYNNTIKVF 178
Cdd:PRK10425  248 TDANARTLF 256
TIGR00010 TIGR00010
hydrolase, TatD family; PSI-BLAST, starting with a urease alpha subunit, finds a large ...
1-178 1.17e-14

hydrolase, TatD family; PSI-BLAST, starting with a urease alpha subunit, finds a large superfamily of proteins, including a number of different enzymes that act as hydrolases at C-N bonds other than peptide bonds (EC 3.5.-.-), many uncharacterized proteins, and the members of this family. Several genomes have multiple paralogs related to this family. However, a set of 17 proteins can be found, one each from 17 of the first 20 genomes, such that each member forms a bidirectional best hit across genomes with all other members of the set. This core set (and one other near-perfect member), but not the other paralogs, form the seed for this model. Additionally, members of the seed alignment and all trusted hits, but not all paralogs, have a conserved motif DxHxH near the amino end. The member from E. coli was recently shown to have DNase activity. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 272852 [Multi-domain]  Cd Length: 252  Bit Score: 69.21  E-value: 1.17e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEFEKGSPDQylagLLSLAenNKGKVVAIGECGLDF------DRLQ 74
Cdd:TIGR00010  33 VAVGTDLEDFLRALELAEKYPNVYAAVGVHPLDVDDDTKEDIKE----LERLA--AHPKVVAIGETGLDYykadeyKRRQ 106
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  75 ---FCpkdTQLNsLKTEANL------------------------------------------------------------ 91
Cdd:TIGR00010 107 eevFR---AQLQ-LAEELNLpviihardaeedvldilreekpkvggvlhcftgdaelakklldlgfyisisgivtfknak 182
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 ---EVLKSIPSEKLMIETDAPWCgvksthagskyinTSFPTKKKwenghclkdRNEPCHIIQILEIMSAVREEDPLELAN 168
Cdd:TIGR00010 183 slrEVVRKIPLERLLVETDSPYL-------------APVPYRGK---------RNEPAFVRYTVEAIAEIKGIDVEELAQ 240
                         250
                  ....*....|
gi 1573307390 169 TLYNNTIKVF 178
Cdd:TIGR00010 241 ITTKNAKRLF 250
 
Name Accession Description Interval E-value
TatD_DNAse cd01310
TatD like proteins; E.coli TatD is a cytoplasmic protein, shown to have magnesium dependent ...
1-179 1.83e-37

TatD like proteins; E.coli TatD is a cytoplasmic protein, shown to have magnesium dependent DNase activity.


Pssm-ID: 238635 [Multi-domain]  Cd Length: 251  Bit Score: 129.23  E-value: 1.83e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEFEkgspDQYLAGLLSLAENNKgkVVAIGECGLDFDRLQFcPKDT 80
Cdd:cd01310    33 IVVGTDLKSSKRALELAKKYDNVYAAVGLHPHDADEHV----DEDLDLLELLAANPK--VVAIGEIGLDYYRDKS-PREV 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  81 QL-------------------------------------------------------------------NSLKTEAN--L 91
Cdd:cd01310   106 QKevfraqlelakelnlpvvihsrdahedvleilkeygppkrgvfhcfsgsaeeakelldlgfyisisgIVTFKNANelR 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 EVLKSIPSEKLMIETDAPWCGVKSTHagskyintsfptkkkwenghclKDRNEPCHIIQILEIMSAVREEDPLELANTLY 171
Cdd:cd01310   186 EVVKEIPLERLLLETDSPYLAPVPFR----------------------GKRNEPAYVKHVAEKIAELKGISVEEVAEVTT 243

                  ....*...
gi 1573307390 172 NNTIKVFF 179
Cdd:cd01310   244 ENAKRLFG 251
TatD COG0084
Tat protein secretion system quality control protein TatD (DNase activity) [Cell motility];
1-178 7.50e-28

Tat protein secretion system quality control protein TatD (DNase activity) [Cell motility];


Pssm-ID: 223162 [Multi-domain]  Cd Length: 256  Bit Score: 104.60  E-value: 7.50e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEfekgSPDQYLAGLLSLAENNKgKVVAIGECGLDFDRLQFCPKDT 80
Cdd:COG0084    35 VVVGTDLEDFKRALELAEKYPNVYAAVGVHPLDADE----HSEEDLEELEQLAEHHP-KVVAIGEIGLDYYWDKEPDKER 109
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  81 Q-----------------------------LNSLKTE----------------------------------------ANL 91
Cdd:COG0084   110 QeevfeaqlelakelnlpviihtrdahedtLEILKEEgapvggvlhcfsgsaeearklldlgfyisisgivtfknaeKLR 189
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 EVLKSIPSEKLMIETDAPWCgvksthagskyinTSFPTKKKwenghclkdRNEPCHIIQILEIMSAVREEDPLELANTLY 171
Cdd:COG0084   190 EVARELPLDRLLLETDAPYL-------------APVPYRGK---------RNEPAYVRHVAEKLAELKGISAEEVAEITT 247

                  ....*..
gi 1573307390 172 NNTIKVF 178
Cdd:COG0084   248 ENAKRLF 254
TatD_DNase pfam01026
TatD related DNase; This family of proteins are related to a large superfamily of ...
1-178 1.09e-25

TatD related DNase; This family of proteins are related to a large superfamily of metalloenzymes. TatD, a member of this family has been shown experimentally to be a DNase enzyme.


Pssm-ID: 425997 [Multi-domain]  Cd Length: 253  Bit Score: 98.87  E-value: 1.09e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMF-FSTVGCHPTRCDEFEkgspDQYLAGLLSLAENnkGKVVAIGECGLDFDRLQFCPKD 79
Cdd:pfam01026  32 VVVGTDLEDFLRVLELAEKYPDRvYAAVGVHPHEADEAS----EDDLEALEKLAEH--PKVVAIGEIGLDYYYVDESPKE 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  80 TQ-----------------------------LNSLKTEANL--------------------------------------- 91
Cdd:pfam01026 106 AQeevfrrqlelakelglpvvihtrdaeedlLEILKEAGAPgargvlhcftgsveearkfldlgfyisisgivtfknakk 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 --EVLKSIPSEKLMIETDAPWCgvksthagskyinTSFPTKKKwenghclkdRNEPCHIIQILEIMSAVREEDPLELANT 169
Cdd:pfam01026 186 lrEVAAAIPLDRLLVETDAPYL-------------APVPYRGK---------RNEPAYVPYVVEKLAELKGISPEEVAEI 243

                  ....*....
gi 1573307390 170 LYNNTIKVF 178
Cdd:pfam01026 244 TTENAERLF 252
PRK10425 PRK10425
3'-5' ssDNA/RNA exonuclease TatD;
1-178 8.25e-15

3'-5' ssDNA/RNA exonuclease TatD;


Pssm-ID: 182449  Cd Length: 258  Bit Score: 70.08  E-value: 8.25e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEFEKGSPDQylagLLSLAEnnKGKVVAIGECGLDFDRlQFCPKDT 80
Cdd:PRK10425   33 LITGTNLRESQQAQKLARQYPSCWSTAGVHPHDSSQWQAATEEA----IIELAA--QPEVVAIGECGLDFNR-NFSTPEE 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  81 Q------------------------------------LNSLK---------TEANL------------------------ 91
Cdd:PRK10425  106 QerafvaqlaiaaelnmpvfmhcrdaherfmallepwLDKLPgavlhcftgTREEMqaclarglyigitgwvcderrgle 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 --EVLKSIPSEKLMIETDAPWCGVKSTHAGskyintsfPTKKkwenghclkdRNEPCHIIQILEIMSAVREEDPLELANT 169
Cdd:PRK10425  186 lrELLPLIPAERLLLETDAPYLLPRDLTPK--------PASR----------RNEPAFLPHILQRIAHWRGEDAAWLAAT 247

                  ....*....
gi 1573307390 170 LYNNTIKVF 178
Cdd:PRK10425  248 TDANARTLF 256
TIGR00010 TIGR00010
hydrolase, TatD family; PSI-BLAST, starting with a urease alpha subunit, finds a large ...
1-178 1.17e-14

hydrolase, TatD family; PSI-BLAST, starting with a urease alpha subunit, finds a large superfamily of proteins, including a number of different enzymes that act as hydrolases at C-N bonds other than peptide bonds (EC 3.5.-.-), many uncharacterized proteins, and the members of this family. Several genomes have multiple paralogs related to this family. However, a set of 17 proteins can be found, one each from 17 of the first 20 genomes, such that each member forms a bidirectional best hit across genomes with all other members of the set. This core set (and one other near-perfect member), but not the other paralogs, form the seed for this model. Additionally, members of the seed alignment and all trusted hits, but not all paralogs, have a conserved motif DxHxH near the amino end. The member from E. coli was recently shown to have DNase activity. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 272852 [Multi-domain]  Cd Length: 252  Bit Score: 69.21  E-value: 1.17e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390   1 MITGGSLQDSKDALQLAQTNDMFFSTVGCHPTRCDEFEKGSPDQylagLLSLAenNKGKVVAIGECGLDF------DRLQ 74
Cdd:TIGR00010  33 VAVGTDLEDFLRALELAEKYPNVYAAVGVHPLDVDDDTKEDIKE----LERLA--AHPKVVAIGETGLDYykadeyKRRQ 106
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  75 ---FCpkdTQLNsLKTEANL------------------------------------------------------------ 91
Cdd:TIGR00010 107 eevFR---AQLQ-LAEELNLpviihardaeedvldilreekpkvggvlhcftgdaelakklldlgfyisisgivtfknak 182
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1573307390  92 ---EVLKSIPSEKLMIETDAPWCgvksthagskyinTSFPTKKKwenghclkdRNEPCHIIQILEIMSAVREEDPLELAN 168
Cdd:TIGR00010 183 slrEVVRKIPLERLLVETDSPYL-------------APVPYRGK---------RNEPAFVRYTVEAIAEIKGIDVEELAQ 240
                         250
                  ....*....|
gi 1573307390 169 TLYNNTIKVF 178
Cdd:TIGR00010 241 ITTKNAKRLF 250
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.20
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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