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Conserved domains on  [gi|38570101|ref|NP_060222|]
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TBC1 domain family member 8B isoform a [Homo sapiens]

Protein Classification

PH-GRAM1_TBC1D8B and PH-GRAM2_TBC1D8B domain-containing protein( domain architecture ID 10193413)

protein containing domains PH-GRAM1_TBC1D8B, PH-GRAM2_TBC1D8B, and TBC

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PH-GRAM1_TBC1D8B cd13350
TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 156-254 1.04e-65

TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8B may act as a GTPase-activating protein for Rab family protein(s). TBC1D8B contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


:

Pssm-ID: 275419  Cd Length: 99  Bit Score: 216.33  E-value: 1.04e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  156 KEKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNVILTESIHVCSQGENHYFSMFL 235
Cdd:cd13350    1 QEKLVTYYSCSYWRGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEISKLEKTSNVILTESIHVCSRGEDHYFSMFL 80

                         90
                 ....*....|....*....
gi 38570101  236 HINQTYLLMEQLANYAIRR 254
Cdd:cd13350   81 HINETFLLMEQLANYAVRR 99
PH-GRAM2_TBC1D8B cd13352
TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 296-388 1.49e-55

TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8B may act as a GTPase-activating protein for Rab family protein(s). TBC1D8B contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


:

Pssm-ID: 270159  Cd Length: 93  Bit Score: 187.36  E-value: 1.49e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  296 GESLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGNQCSVIIPLREVLAIDKTNDSSKSVIISIKGKTAFRFHEVK 375
Cdd:cd13352    1 EETLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGSLCSVIIPLREVLSIDKTDDSSRAVTISTKGKRAFRFTEVK 80

                         90
                 ....*....|...
gi 38570101  376 DFEQLVAKLRLRC 388
Cdd:cd13352   81 DFEQLVAKLRLKC 93
TBC smart00164
Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs; Widespread domain present in Gyp6 and ...
 486-694 1.39e-53

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs; Widespread domain present in Gyp6 and Gyp7, thereby giving rise to the notion that it performs a GTP-activator activity on Rab-like GTPases.


:

Pssm-ID: 214540 [Multi-domain]  Cd Length: 216  Bit Score: 186.36  E-value: 1.39e-53
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101     486 RGIPETLRGELWMLFSGA-VNDMATNPDYYTEVVEQSLGTCNLATEEIERDLRRSLPEHPAFQSDTG--ISALRRVLTAY 562
Cdd:smart00164    3 KGVPPSLRGVVWKLLLNAqPMDTSADKDLYSRLLKETAPDDKSIVHQIEKDLRRTFPEHSFFQDKEGpgQESLRRVLKAY 82

                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101     563 AYRNPKIGYCQAMNILTSVLLLY-AKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIRDHLPQLTEHMTDMTF 641
Cdd:smart00164   83 ALYNPEVGYCQGMNFLAAPLLLVmEDEEDAFWCLVKLMERYGPNFYLPDMSGLQLDLLQLDRLVKEYDPDLYKHLKDLGI 162

                           170       180       190       200       210
                    ....*....|....*....|....*....|....*....|....*....|....
gi 38570101     642 -FSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAILDYNLDKLL 694
Cdd:smart00164  163 tPSLYALRWFLTLFARELPLEIVLRIWDVLFAEGSDFLFRVALALLKLHRDVLL 216
 
Name Accession Description Interval E-value
PH-GRAM1_TBC1D8B cd13350
TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 156-254 1.04e-65

TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8B may act as a GTPase-activating protein for Rab family protein(s). TBC1D8B contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275419  Cd Length: 99  Bit Score: 216.33  E-value: 1.04e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  156 KEKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNVILTESIHVCSQGENHYFSMFL 235
Cdd:cd13350    1 QEKLVTYYSCSYWRGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEISKLEKTSNVILTESIHVCSRGEDHYFSMFL 80

                         90
                 ....*....|....*....
gi 38570101  236 HINQTYLLMEQLANYAIRR 254
Cdd:cd13350   81 HINETFLLMEQLANYAVRR 99
PH-GRAM2_TBC1D8B cd13352
TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 296-388 1.49e-55

TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8B may act as a GTPase-activating protein for Rab family protein(s). TBC1D8B contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270159  Cd Length: 93  Bit Score: 187.36  E-value: 1.49e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  296 GESLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGNQCSVIIPLREVLAIDKTNDSSKSVIISIKGKTAFRFHEVK 375
Cdd:cd13352    1 EETLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGSLCSVIIPLREVLSIDKTDDSSRAVTISTKGKRAFRFTEVK 80

                         90
                 ....*....|...
gi 38570101  376 DFEQLVAKLRLRC 388
Cdd:cd13352   81 DFEQLVAKLRLKC 93
TBC smart00164
Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs; Widespread domain present in Gyp6 and ...
 486-694 1.39e-53

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs; Widespread domain present in Gyp6 and Gyp7, thereby giving rise to the notion that it performs a GTP-activator activity on Rab-like GTPases.


Pssm-ID: 214540 [Multi-domain]  Cd Length: 216  Bit Score: 186.36  E-value: 1.39e-53
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101     486 RGIPETLRGELWMLFSGA-VNDMATNPDYYTEVVEQSLGTCNLATEEIERDLRRSLPEHPAFQSDTG--ISALRRVLTAY 562
Cdd:smart00164    3 KGVPPSLRGVVWKLLLNAqPMDTSADKDLYSRLLKETAPDDKSIVHQIEKDLRRTFPEHSFFQDKEGpgQESLRRVLKAY 82

                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101     563 AYRNPKIGYCQAMNILTSVLLLY-AKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIRDHLPQLTEHMTDMTF 641
Cdd:smart00164   83 ALYNPEVGYCQGMNFLAAPLLLVmEDEEDAFWCLVKLMERYGPNFYLPDMSGLQLDLLQLDRLVKEYDPDLYKHLKDLGI 162

                           170       180       190       200       210
                    ....*....|....*....|....*....|....*....|....*....|....
gi 38570101     642 -FSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAILDYNLDKLL 694
Cdd:smart00164  163 tPSLYALRWFLTLFARELPLEIVLRIWDVLFAEGSDFLFRVALALLKLHRDVLL 216
COG5210 COG5210
GTPase-activating protein [General function prediction only];
399-738 3.99e-46

GTPase-activating protein [General function prediction only];


Pssm-ID: 227535 [Multi-domain]  Cd Length: 496  Bit Score: 173.83  E-value: 3.99e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  399 STELAISSESTEPSDNFEV---QSLTSQRECSKTVNTEALMTVFHPQNLETLNSKMLKEKmkeqswkiLFAECGRGVSMF 475
Cdd:COG5210  129 SLPTHLPEASSTEKDFSSFkgsSSLNSNPELNKEINELSLKEEPQKLRYYELAADKLWIS--------YLDPNPLSFLPV 200

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  476 RTKKTRDLVVRGIPETLRGELWMLFSGAVNDMATNPDYY---TEVVEQSLGTCNLATEEIERDLRRSLPEHPAFQSDTGI 552
Cdd:COG5210  201 QLSKLRELIRKGIPNELRGDVWEFLLGIGFDLDKNPGLYerlLNLHREAKIPTQEIISQIEKDLSRTFPDNSLFQTEISI 280

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  553 SA--LRRVLTAYAYRNPKIGYCQAMNILTSVLLLY-AKEEEAFWLLVAVCER-MLPDYFNRRIIGALVDQAVFEELIRDH 628
Cdd:COG5210  281 RAenLRRVLKAYSLYNPEVGYVQGMNFLAAPLLLVlESEEQAFWCLVKLLKNyGLPGYFLKNLSGLHRDLKVLDDLVEEL 360

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  629 LPQLTEHM--TDMTfFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAILDYNLDKLLTCKDDAEAVTAL 706
Cdd:COG5210  361 DPELYEHLlrEGVV-LLMFAFRWFLTLFVREFPLEYALRIWDCLFLEGSSMLFQLALAILKLLRDKLLKLDSDELLDLLL 439

                        330       340       350
                 ....*....|....*....|....*....|..
gi 38570101  707 NRFFDNVTNKDSPLPSNVQQGSNVSDEKTSHT 738
Cdd:COG5210  440 KQLFLHSGKEAWSSILKFRHGTDRDILLFIED 471
RabGAP-TBC pfam00566
Rab-GTPase-TBC domain; Identification of a TBC domain in GYP6_YEAST and GYP7_YEAST, which are ...
 532-694 1.51e-42

Rab-GTPase-TBC domain; Identification of a TBC domain in GYP6_YEAST and GYP7_YEAST, which are GTPase activator proteins of yeast Ypt6 and Ypt7, implies that these domains are GTPase activator proteins of Rab-like small GTPases.


Pssm-ID: 459855  Cd Length: 178  Bit Score: 153.18  E-value: 1.51e-42
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101    532 IERDLRRSLPEHPAFQSDTGISALRRVLTAYAYRNPKIGYCQAMNILTSVLLL-YAKEEEAFWLLVAVCER-MLPDYFNR 609
Cdd:pfam00566   12 IEKDVPRTFPHSFFFDNGPGQNSLRRILKAYSIYNPDVGYCQGMNFIAAPLLLvYLDEEDAFWCFVSLLENyLLRDFYTP 91

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101    610 RIIGALVDQAVFEELIRDHLPQLTEHMT----DMTFFSSvslSWFLTLFISVLPIESAVNVVDCFFYDGIKA-ILQLGLA 684
Cdd:pfam00566   92 DFPGLKRDLYVFEELLKKKLPKLYKHLKelglDPDLFAS---QWFLTLFAREFPLSTVLRIWDYFFLEGEKFvLFRVALA 168

                          170
                   ....*....|
gi 38570101    685 ILDYNLDKLL 694
Cdd:pfam00566  169 ILKRFREELL 178
GRAM pfam02893
GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other ...
 146-251 1.83e-14

GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.


Pssm-ID: 397160  Cd Length: 112  Bit Score: 70.47  E-value: 1.83e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101    146 KFEKCFGLPEKEKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSN--VILTESIHVC 223
Cdd:pfam02893    2 LFRKKFKLPPEERLIASYSCYLNRDGGPVQGRLYLTNYRLCFRSLPKGWSTKVVIPLVDIEEIEKLKGgaNLFPNGIQVE 81

                           90       100
                   ....*....|....*....|....*...
gi 38570101    224 SqGENHYFSMFLHINQTYLLMEQLANYA 251
Cdd:pfam02893   82 T-GSNDKFSFAGFVTRDEAIEFILALLK 108
GRAM smart00568
domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;
152-212 1.40e-13

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;


Pssm-ID: 214725 [Multi-domain]  Cd Length: 60  Bit Score: 66.46  E-value: 1.40e-13
                            10        20        30        40        50        60
                    ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 38570101     152 GLPEKEKLVTYYSCSYWKgRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTS 212
Cdd:smart00568    1 KLPEEEKLIADYSCYLSR-TGPVQGRLYISNYRLCFRSNLPGKLTKVVIPLADITRIEKST 60
GRAM pfam02893
GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other ...
 287-371 8.65e-10

GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.


Pssm-ID: 397160  Cd Length: 112  Bit Score: 57.38  E-value: 8.65e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101    287 FNAFFRLPKGESLKEVHECFLWVPFSHfnTHGKMCISENYICFASQ-DGNQCSVIIPLREVLAIDKTNDSS----KSVII 361
Cdd:pfam02893    3 FRKKFKLPPEERLIASYSCYLNRDGGP--VQGRLYLTNYRLCFRSLpKGWSTKVVIPLVDIEEIEKLKGGAnlfpNGIQV 80

                           90
                   ....*....|
gi 38570101    362 SIKGKTAFRF 371
Cdd:pfam02893   81 ETGSNDKFSF 90
GRAM smart00568
domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;
292-351 2.20e-08

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;


Pssm-ID: 214725 [Multi-domain]  Cd Length: 60  Bit Score: 51.44  E-value: 2.20e-08
                            10        20        30        40        50        60
                    ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 38570101     292 RLPKGESLKEVHECFLW--VPFshfntHGKMCISENYICFASQD-GNQCSVIIPLREVLAIDK 351
Cdd:smart00568    1 KLPEEEKLIADYSCYLSrtGPV-----QGRLYISNYRLCFRSNLpGKLTKVVIPLADITRIEK 58
 
Name Accession Description Interval E-value
PH-GRAM1_TBC1D8B cd13350
TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 156-254 1.04e-65

TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8B may act as a GTPase-activating protein for Rab family protein(s). TBC1D8B contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275419  Cd Length: 99  Bit Score: 216.33  E-value: 1.04e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  156 KEKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNVILTESIHVCSQGENHYFSMFL 235
Cdd:cd13350    1 QEKLVTYYSCSYWRGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEISKLEKTSNVILTESIHVCSRGEDHYFSMFL 80

                         90
                 ....*....|....*....
gi 38570101  236 HINQTYLLMEQLANYAIRR 254
Cdd:cd13350   81 HINETFLLMEQLANYAVRR 99
PH-GRAM2_TBC1D8B cd13352
TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 296-388 1.49e-55

TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8B may act as a GTPase-activating protein for Rab family protein(s). TBC1D8B contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270159  Cd Length: 93  Bit Score: 187.36  E-value: 1.49e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  296 GESLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGNQCSVIIPLREVLAIDKTNDSSKSVIISIKGKTAFRFHEVK 375
Cdd:cd13352    1 EETLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGSLCSVIIPLREVLSIDKTDDSSRAVTISTKGKRAFRFTEVK 80

                         90
                 ....*....|...
gi 38570101  376 DFEQLVAKLRLRC 388
Cdd:cd13352   81 DFEQLVAKLRLKC 93
TBC smart00164
Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs; Widespread domain present in Gyp6 and ...
 486-694 1.39e-53

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs; Widespread domain present in Gyp6 and Gyp7, thereby giving rise to the notion that it performs a GTP-activator activity on Rab-like GTPases.


Pssm-ID: 214540 [Multi-domain]  Cd Length: 216  Bit Score: 186.36  E-value: 1.39e-53
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101     486 RGIPETLRGELWMLFSGA-VNDMATNPDYYTEVVEQSLGTCNLATEEIERDLRRSLPEHPAFQSDTG--ISALRRVLTAY 562
Cdd:smart00164    3 KGVPPSLRGVVWKLLLNAqPMDTSADKDLYSRLLKETAPDDKSIVHQIEKDLRRTFPEHSFFQDKEGpgQESLRRVLKAY 82

                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101     563 AYRNPKIGYCQAMNILTSVLLLY-AKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIRDHLPQLTEHMTDMTF 641
Cdd:smart00164   83 ALYNPEVGYCQGMNFLAAPLLLVmEDEEDAFWCLVKLMERYGPNFYLPDMSGLQLDLLQLDRLVKEYDPDLYKHLKDLGI 162

                           170       180       190       200       210
                    ....*....|....*....|....*....|....*....|....*....|....
gi 38570101     642 -FSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAILDYNLDKLL 694
Cdd:smart00164  163 tPSLYALRWFLTLFARELPLEIVLRIWDVLFAEGSDFLFRVALALLKLHRDVLL 216
PH-GRAM1_TCB1D8_TCB1D9_family cd13217
TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators ...
 157-254 1.75e-48

TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8, TBC1D8B, TBC1D9 and TBC1D9B may act as a GTPase-activating proteins for Rab family protein(s). They all contain an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275404  Cd Length: 99  Bit Score: 167.23  E-value: 1.75e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  157 EKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNVILTESIHVCSQGENHYFSMFLH 236
Cdd:cd13217    2 EKLVTYYSCSYWKGRVPRQGWLYLSINHLCFYSFLLGSESKLIIRWTEVSDLERTSNTILTDTIKVNTRSKEHPFSMFLN 81

                         90
                 ....*....|....*...
gi 38570101  237 INQTYLLMEQLANYAIRR 254
Cdd:cd13217   82 ISETFKLMEQLAQIPDRP 99
PH-GRAM1_TCB1D9_TCB1D9B cd13351
TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin ...
 157-254 6.51e-48

TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D9 and TCB1D9B may act as a GTPase-activating proteins for Rab family protein(s). TBC1D9 and TCB1D9B contain two N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275420  Cd Length: 99  Bit Score: 165.64  E-value: 6.51e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  157 EKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNVILTESIHVCSQGENHYFSMFLH 236
Cdd:cd13351    2 EKLVNYYSCSYWKGRVPRQGWLYLSVNHLCFYSFLLGKEAKLVIRWTDVTQLEKNNSLLLPDSIKVVTRDKEHYFSMFLN 81

                         90
                 ....*....|....*...
gi 38570101  237 INQTYLLMEQLANYAIRR 254
Cdd:cd13351   82 ISETFKLMEQLANLAMRQ 99
COG5210 COG5210
GTPase-activating protein [General function prediction only];
399-738 3.99e-46

GTPase-activating protein [General function prediction only];


Pssm-ID: 227535 [Multi-domain]  Cd Length: 496  Bit Score: 173.83  E-value: 3.99e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  399 STELAISSESTEPSDNFEV---QSLTSQRECSKTVNTEALMTVFHPQNLETLNSKMLKEKmkeqswkiLFAECGRGVSMF 475
Cdd:COG5210  129 SLPTHLPEASSTEKDFSSFkgsSSLNSNPELNKEINELSLKEEPQKLRYYELAADKLWIS--------YLDPNPLSFLPV 200

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  476 RTKKTRDLVVRGIPETLRGELWMLFSGAVNDMATNPDYY---TEVVEQSLGTCNLATEEIERDLRRSLPEHPAFQSDTGI 552
Cdd:COG5210  201 QLSKLRELIRKGIPNELRGDVWEFLLGIGFDLDKNPGLYerlLNLHREAKIPTQEIISQIEKDLSRTFPDNSLFQTEISI 280

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  553 SA--LRRVLTAYAYRNPKIGYCQAMNILTSVLLLY-AKEEEAFWLLVAVCER-MLPDYFNRRIIGALVDQAVFEELIRDH 628
Cdd:COG5210  281 RAenLRRVLKAYSLYNPEVGYVQGMNFLAAPLLLVlESEEQAFWCLVKLLKNyGLPGYFLKNLSGLHRDLKVLDDLVEEL 360

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  629 LPQLTEHM--TDMTfFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAILDYNLDKLLTCKDDAEAVTAL 706
Cdd:COG5210  361 DPELYEHLlrEGVV-LLMFAFRWFLTLFVREFPLEYALRIWDCLFLEGSSMLFQLALAILKLLRDKLLKLDSDELLDLLL 439

                        330       340       350
                 ....*....|....*....|....*....|..
gi 38570101  707 NRFFDNVTNKDSPLPSNVQQGSNVSDEKTSHT 738
Cdd:COG5210  440 KQLFLHSGKEAWSSILKFRHGTDRDILLFIED 471
PH-GRAM2_TCB1D8_TCB1D9_family cd13218
TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators ...
 297-388 4.26e-45

TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8, TBC1D8B, TBC1D9 and TBC1D9B may act as a GTPase-activating proteins for Rab family protein(s). They all contain an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275405  Cd Length: 96  Bit Score: 157.37  E-value: 4.26e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  297 ESLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGNQCSVIIPLREVLAIDKTNDSS---KSVIISIKGKTAFRFHE 373
Cdd:cd13218    2 EKLKEVHDCFLWTPFSHFHTHGKMFISENYICFASKEGNLCSVIIPLREVLAIEKTNDSSvlpKPVIISIKGKMAFRFSE 81

                         90
                 ....*....|....*
gi 38570101  374 VKDFEQLVAKLRLRC 388
Cdd:cd13218   82 LKDRDELVAKLRLKL 96
RabGAP-TBC pfam00566
Rab-GTPase-TBC domain; Identification of a TBC domain in GYP6_YEAST and GYP7_YEAST, which are ...
 532-694 1.51e-42

Rab-GTPase-TBC domain; Identification of a TBC domain in GYP6_YEAST and GYP7_YEAST, which are GTPase activator proteins of yeast Ypt6 and Ypt7, implies that these domains are GTPase activator proteins of Rab-like small GTPases.


Pssm-ID: 459855  Cd Length: 178  Bit Score: 153.18  E-value: 1.51e-42
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101    532 IERDLRRSLPEHPAFQSDTGISALRRVLTAYAYRNPKIGYCQAMNILTSVLLL-YAKEEEAFWLLVAVCER-MLPDYFNR 609
Cdd:pfam00566   12 IEKDVPRTFPHSFFFDNGPGQNSLRRILKAYSIYNPDVGYCQGMNFIAAPLLLvYLDEEDAFWCFVSLLENyLLRDFYTP 91

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101    610 RIIGALVDQAVFEELIRDHLPQLTEHMT----DMTFFSSvslSWFLTLFISVLPIESAVNVVDCFFYDGIKA-ILQLGLA 684
Cdd:pfam00566   92 DFPGLKRDLYVFEELLKKKLPKLYKHLKelglDPDLFAS---QWFLTLFAREFPLSTVLRIWDYFFLEGEKFvLFRVALA 168

                          170
                   ....*....|
gi 38570101    685 ILDYNLDKLL 694
Cdd:pfam00566  169 ILKRFREELL 178
PH-GRAM1_TBC1D8 cd13349
TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) ...
 157-254 5.80e-38

TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8 may act as a GTPase-activating protein for Rab family protein(s). TBC1D8 contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270156  Cd Length: 99  Bit Score: 137.29  E-value: 5.80e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  157 EKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNVILTESIHVCSQGENHYFSMFLH 236
Cdd:cd13349    2 EKLVTYYSCCCWKGRVPRQGWLYLSINHLCFYSFFLGKELKLLIPWVDVQKLERTSNVFMTDTIRVTTQNKERDFSMFLN 81

                         90
                 ....*....|....*...
gi 38570101  237 INQTYLLMEQLANYAIRR 254
Cdd:cd13349   82 IDEVFRIMEQLADVTLRR 99
PH-GRAM2_TBC1D8 cd13353
TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) ...
 297-387 2.13e-22

TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8 may act as a GTPase-activating protein for Rab family protein(s). TBC1D8 contains two N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270160  Cd Length: 96  Bit Score: 92.60  E-value: 2.13e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  297 ESLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGNQCSVIIPLREVLAIDKTNDSS---KSVIISIKGKTAFRFHE 373
Cdd:cd13353    2 EKLHEVVDCSLWTPFSRCHTAGRMYTSDSYICFASKEDGSCNVILPLREVVSIEKMEDTSllpNPIIVSIRSKMAFQFIE 81

                         90
                 ....*....|....
gi 38570101  374 VKDFEQLVAKLRLR 387
Cdd:cd13353   82 LKDRDSLVESLLAR 95
PH-GRAM_GRAMDC cd13220
GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 157-247 7.54e-17

GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; The GRAMDC proteins are membrane proteins. Nothing is known about its function. Members include: GRAMDC1A, GRAMDC1B, GRAMDC1C, GRAMDC2, GRAMDC3, GRAMDC4, and GRAMDC-like proteins. All of the members, except for GRAMDC4 are included in this hierarchy. Each contains a single PH-GRAM domain at their N-terminus. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275406  Cd Length: 94  Bit Score: 76.78  E-value: 7.54e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  157 EKLVTYYSCSyWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNV-ILTESIHVCSQGENHYFSMFL 235
Cdd:cd13220    3 ERLIDDFSCA-LQRDILLQGRLYISENHLCFYSNIFGWETKLVIPFKDITSIEKKKTAlIFPNAIEITTKGEKYFFTSFL 81

                         90
                 ....*....|..
gi 38570101  236 HINQTYLLMEQL 247
Cdd:cd13220   82 SRDSAYKLLTRV 93
PH-GRAM2_TCB1D9_TCB1D9B cd13354
TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin ...
 297-383 2.05e-16

TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D9 and TCB1D9B may act as a GTPase-activating proteins for Rab family protein(s). TBC1D9 and TCB1D9B contain two N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270161  Cd Length: 97  Bit Score: 75.76  E-value: 2.05e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  297 ESLKEVHECFLWVPFSHFNTHGKMCISENYICFASQDGNQCSVIIPLREVLAIDKTNDSS----KSVIISIKGKTAFRFH 372
Cdd:cd13354    2 EKLDGSTDCTLWTPYNKRHVWGTLYLSQNYICFTSKVRDLVSLVIPLREVTSVEKADSSSvslpNGILITTKSKMTFLFA 81

                         90
                 ....*....|.
gi 38570101  373 EVKDFEQLVAK 383
Cdd:cd13354   82 QIKDRDFLVHK 92
GRAM pfam02893
GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other ...
 146-251 1.83e-14

GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.


Pssm-ID: 397160  Cd Length: 112  Bit Score: 70.47  E-value: 1.83e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101    146 KFEKCFGLPEKEKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSN--VILTESIHVC 223
Cdd:pfam02893    2 LFRKKFKLPPEERLIASYSCYLNRDGGPVQGRLYLTNYRLCFRSLPKGWSTKVVIPLVDIEEIEKLKGgaNLFPNGIQVE 81

                           90       100
                   ....*....|....*....|....*...
gi 38570101    224 SqGENHYFSMFLHINQTYLLMEQLANYA 251
Cdd:pfam02893   82 T-GSNDKFSFAGFVTRDEAIEFILALLK 108
GRAM smart00568
domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;
152-212 1.40e-13

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;


Pssm-ID: 214725 [Multi-domain]  Cd Length: 60  Bit Score: 66.46  E-value: 1.40e-13
                            10        20        30        40        50        60
                    ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 38570101     152 GLPEKEKLVTYYSCSYWKgRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTS 212
Cdd:smart00568    1 KLPEEEKLIADYSCYLSR-TGPVQGRLYISNYRLCFRSNLPGKLTKVVIPLADITRIEKST 60
GRAM pfam02893
GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other ...
 287-371 8.65e-10

GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.


Pssm-ID: 397160  Cd Length: 112  Bit Score: 57.38  E-value: 8.65e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101    287 FNAFFRLPKGESLKEVHECFLWVPFSHfnTHGKMCISENYICFASQ-DGNQCSVIIPLREVLAIDKTNDSS----KSVII 361
Cdd:pfam02893    3 FRKKFKLPPEERLIASYSCYLNRDGGP--VQGRLYLTNYRLCFRSLpKGWSTKVVIPLVDIEEIEKLKGGAnlfpNGIQV 80

                           90
                   ....*....|
gi 38570101    362 SIKGKTAFRF 371
Cdd:pfam02893   81 ETGSNDKFSF 90
GRAM smart00568
domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;
292-351 2.20e-08

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;


Pssm-ID: 214725 [Multi-domain]  Cd Length: 60  Bit Score: 51.44  E-value: 2.20e-08
                            10        20        30        40        50        60
                    ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 38570101     292 RLPKGESLKEVHECFLW--VPFshfntHGKMCISENYICFASQD-GNQCSVIIPLREVLAIDK 351
Cdd:smart00568    1 KLPEEEKLIADYSCYLSrtGPV-----QGRLYISNYRLCFRSNLpGKLTKVVIPLADITRIEK 58
PH-GRAM cd10570
Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins ...
 157-210 6.30e-08

Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; Myotubularin-related proteins are a subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3-phosphorylated inositol lipids. Mutations in this family cause the human neuromuscular disorders myotubular myopathy and type 4B Charcot-Marie-Tooth syndrome. 6 of the 13 MTMRs (MTMRs 5, 9-13) contain naturally occurring substitutions of residues required for catalysis by PTP family enzymes. Although these proteins are predicted to be enzymatically inactive, they are thought to function as antagonists of endogenous phosphatase activity or interaction modules. Most MTMRs contain a N-terminal PH-GRAM domain, a Rac-induced recruitment domain (RID) domain, a PTP domain (which may be active or inactive), a SET-interaction domain, and a C-terminal coiled-coil region. In addition some members contain DENN domain N-terminal to the PH-GRAM domain and FYVE, PDZ, and PH domains C-terminal to the coiled-coil region. The GRAM domain, found in myotubularins, glucosyltransferases, and other putative membrane-associated proteins, is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275393  Cd Length: 94  Bit Score: 51.23  E-value: 6.30e-08
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 38570101  157 EKLVTYYSCSYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEK 210
Cdd:cd10570    2 EKLGVRFCCALRPRKLPLEGTLYLSTYRLIFSSKADGDETKLVIPLVDITDVEK 55
PH-GRAM_GRAMDC cd13220
GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 317-353 1.19e-04

GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; The GRAMDC proteins are membrane proteins. Nothing is known about its function. Members include: GRAMDC1A, GRAMDC1B, GRAMDC1C, GRAMDC2, GRAMDC3, GRAMDC4, and GRAMDC-like proteins. All of the members, except for GRAMDC4 are included in this hierarchy. Each contains a single PH-GRAM domain at their N-terminus. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275406  Cd Length: 94  Bit Score: 42.11  E-value: 1.19e-04
                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 38570101  317 HGKMCISENYICFASQ-DGNQCSVIIPLREVLAIDKTN 353
Cdd:cd13220   20 QGRLYISENHLCFYSNiFGWETKLVIPFKDITSIEKKK 57
PH-GRAM_C2-GRAM cd13219
C2 and GRAM domain-containing protein Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 157-220 1.01e-03

C2 and GRAM domain-containing protein Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; C2GRAM contains two N-terminal C2 domains followed by a single PH-GRAM domain. Since it contains both of these domains it is assumed that this gene cross-links both calcium and phosphoinositide signaling pathways. In general he C2 domain is involved in binding phospholipids in a calcium dependent manner or calcium independent manner. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270039 [Multi-domain]  Cd Length: 111  Bit Score: 39.76  E-value: 1.01e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 38570101  157 EKLVTYYSCSYwKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEVSKLEKTSNVILTESI 220
Cdd:cd13219    2 EFLINDFSCAL-KRKFLYQGRMFLSARHIGFHSNVFGKKTKFVFLWEDIEEIQESPHSLINPAI 64
PH-GRAM2_AGT26 cd13216
Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, ...
 156-200 1.51e-03

Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 2; ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275403  Cd Length: 93  Bit Score: 38.72  E-value: 1.51e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 38570101  156 KEKLVTYYSCsYWKGRVPCQGWLYLSTNFLSFYSFLLGSEIKLII 200
Cdd:cd13216    1 TEKLIASYYC-YLIRVLPVYGKLYVSNNYLCFRSLLPGVSTKMIL 44
PH-GRAM cd10570
Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins ...
 318-351 7.37e-03

Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; Myotubularin-related proteins are a subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3-phosphorylated inositol lipids. Mutations in this family cause the human neuromuscular disorders myotubular myopathy and type 4B Charcot-Marie-Tooth syndrome. 6 of the 13 MTMRs (MTMRs 5, 9-13) contain naturally occurring substitutions of residues required for catalysis by PTP family enzymes. Although these proteins are predicted to be enzymatically inactive, they are thought to function as antagonists of endogenous phosphatase activity or interaction modules. Most MTMRs contain a N-terminal PH-GRAM domain, a Rac-induced recruitment domain (RID) domain, a PTP domain (which may be active or inactive), a SET-interaction domain, and a C-terminal coiled-coil region. In addition some members contain DENN domain N-terminal to the PH-GRAM domain and FYVE, PDZ, and PH domains C-terminal to the coiled-coil region. The GRAM domain, found in myotubularins, glucosyltransferases, and other putative membrane-associated proteins, is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275393  Cd Length: 94  Bit Score: 36.98  E-value: 7.37e-03
                         10        20        30
                 ....*....|....*....|....*....|....*
gi 38570101  318 GKMCISENYICF-ASQDGNQCSVIIPLREVLAIDK 351
Cdd:cd10570   21 GTLYLSTYRLIFsSKADGDETKLVIPLVDITDVEK 55
PH-GRAM2_TCB1D9_TCB1D9B cd13354
TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin ...
 157-237 7.54e-03

TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D9 and TCB1D9B may act as a GTPase-activating proteins for Rab family protein(s). TBC1D9 and TCB1D9B contain two N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270161  Cd Length: 97  Bit Score: 36.86  E-value: 7.54e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 38570101  157 EKLVTYYSCSYW----KGRVPcqGWLYLSTNFLSFYSFLLgSEIKLIISWDEVSKLEK--TSNVILTESIHVCSQGENHY 230
Cdd:cd13354    2 EKLDGSTDCTLWtpynKRHVW--GTLYLSQNYICFTSKVR-DLVSLVIPLREVTSVEKadSSSVSLPNGILITTKSKMTF 78


                 ....*..
gi 38570101  231 fsMFLHI 237
Cdd:cd13354   79 --LFAQI 83
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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