calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  23 DEELEERLVEWIVMQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAAE 102
Cdd:cd21279   1 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVVLSKLVNSLYPDGSKPVKIPDNPPSMVFKQMEQVAQFLKAAE 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 13928744 103 DYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGSLAVTKNDG 143
Cdd:cd21279  81 DYGVVKTDMFQTVDLYEGKDMAAVQRTLVALGSLAVTKNDG 121

calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  23 DEELEERLVEWIVMQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAAE 102
Cdd:cd21279   1 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVVLSKLVNSLYPDGSKPVKIPDNPPSMVFKQMEQVAQFLKAAE 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 13928744 103 DYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGSLAVTKNDG 143
Cdd:cd21279  81 DYGVVKTDMFQTVDLYEGKDMAAVQRTLVALGSLAVTKNDG 121

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744    24 EELEERLVEWIVMQCGPDVGRPDRGrlGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPpsmvFKQMEQVAQFLKAAE- 102
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPGVRVT--NFTTDLRDGLALCALLNKLAPGLVDKKKLNKSE----FDKLENINLALDVAEk 74

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 13928744   103 DYGVTKTDMfQTVDLFEGKDMaAVQRTVMALGSLAVT 139
Cdd:pfam00307  75 KLGVPKVLI-EPEDLVEGDNK-SVLTYLASLFRRFQA 109

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 13928744   175 IGLQMGSNRGASQAGMTGYGRPRQI 199
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  23 DEELEERLVEWIVMQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAAE 102
Cdd:cd21279   1 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVVLSKLVNSLYPDGSKPVKIPDNPPSMVFKQMEQVAQFLKAAE 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 13928744 103 DYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGSLAVTKNDG 143
Cdd:cd21279  81 DYGVVKTDMFQTVDLYEGKDMAAVQRTLVALGSLAVTKNDG 121

calponin homology (CH) domain found in transgelin-2; Transgelin-2, also called epididymis tissue protein Li 7e, or SM22-alpha homolog, acts as an actin-binding protein that induces actin gelation and regulates the actin cytoskeleton. It may participate in the development and progression of multiple cancers. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  18 IEKKYDEELEERLVEWIVMQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPEGSKPV-KVPENppSMVFKQMEQVAQ 96
Cdd:cd21280   1 IDKQYDADLEQILIQWITAQCGKQVGRPQPGRENFQNWLKDGTVLCHLINSLYPKGQAPVkKIQAS--TMAFKQMEQISQ 78

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 13928744  97 FLKAAEDYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGSLAVTKNDGHYRGDPNWFMKK 155
Cdd:cd21280  79 FLQAAERYGINTTDIFQTVDLWEGKNMASVQRTLMNLGGLAVTRDDGLFVGDPNWFPKK 137

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  21 KYDEELEERLVEWI--VMQCGPDVGRPdrgrlgFQVWLKNGVILSKLVNSLYPeGSKPvKVpeNPPSMVFKQMEQVAQFL 98
Cdd:cd21207   1 KRDPALEAEALDWIeaVTGEKLDDGKD------YEDVLKDGVILCKLINILKP-GSVK-KI--NTSKMAFKLMENIENFL 70

                        90       100       110
                ....*....|....*....|....*....|....*..
gi 13928744  99 KAAEDYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGS 135
Cdd:cd21207  71 TACKGYGVPKTDLFQTVDLYEKKNIPQVTNCLFALGR 107

Calponin [Cytoskeleton];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  14 VQSKIEKKYDEELEERLVEWIVMQCGPDVGRPdrgrLGFQVWLKNGVILSKLVNSlypegSKPVKVPENPPSMVFKQMEQ 93
Cdd:COG5199   2 VNTKNRCPGMDKQQKEVTLWIETVLGEKFEPP----GDLLSLLKDGVRLCRILNE-----ASPLDIKYKESKMPFVQMEN 72

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  94 VAQFLKAAEDYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGSLAVTKNDGHYRG-DPNWFMKKaqehKREFTDSQ-LQEG 171
Cdd:COG5199  73 ISSFINGLKKLRVPEYELFQTNDLFEAKDLRQVVICLYSLSRYAQKERMFSGPFlGPHLATKK----PRVFSSQEvLDRS 148

                       170
                ....*....|....*...
gi 13928744 172 KHVIGLQMGSNRGASQAG 189
Cdd:COG5199 149 KGAIHLQYGYSDLSEQST 166

calponin homology (CH) domain found in calponin-3; Calponin-3 (CNN3), also called acidic isoform calponin, is an F-actin-binding protein that is expressed in the brain and has been shown to control dendritic spine morphology, density, and plasticity by regulating actin cytoskeletal reorganization and dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  21 KYDEELEERLVEWIVMQCGPDVGRpdrgrlGFQVWLKNGVILSKLVNSLYPEGSKPVkvpeNPPSMVFKQMEQVAQFLKA 100
Cdd:cd21284   1 KYDPQKEEELRNWIEEVTGMSIGE------NFQKGLKDGVILCELINKLQPGSIRKI----NESKLNWHQLENIGNFIKA 70

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
gi 13928744 101 AEDYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGSLAVTK 140
Cdd:cd21284  71 IQAYGMKPHDIFEANDLFENGNMTQVQTTLLALAGLAKTK 110

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744    24 EELEERLVEWIVMQCGPDVGRPDRGrlGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPpsmvFKQMEQVAQFLKAAE- 102
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPGVRVT--NFTTDLRDGLALCALLNKLAPGLVDKKKLNKSE----FDKLENINLALDVAEk 74

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 13928744   103 DYGVTKTDMfQTVDLFEGKDMaAVQRTVMALGSLAVT 139
Cdd:pfam00307  75 KLGVPKVLI-EPEDLVEGDNK-SVLTYLASLFRRFQA 109

calponin homology (CH) domain found in calponin-1 and similar proteins; Calponin-1 (CNN1), also called basic calponin, or smooth muscle calponin H1, is a thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C, and tropomyosin. Calponin-1 contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  23 DEELEERLVEWIVMQCGPDVGRpdrgrlGFQVWLKNGVILSKLVNSLYPeGSKPvKVpeNPPSMVFKQMEQVAQFLKAAE 102
Cdd:cd21282   1 DPQTEEELRVWIEGVTGRRIGD------NFMDGLKDGVILCELINKLQP-GSVR-KI--NESTQNWHKLENIGNFIKAIM 70

                        90       100       110
                ....*....|....*....|....*....|....*...
gi 13928744 103 DYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGSLAVTK 140
Cdd:cd21282  71 HYGVKPHDIFEANDLFENTNHTQVQSTLIALASMAKTK 108

calponin homology (CH) domain found in the growth arrest-specific protein 2 family; The growth arrest-specific protein 2 (GAS-2) family includes GAS-2, and GAS-2 like proteins, GAS2L1-3. GAS-2 may play a role in apoptosis by acting as a cell death substrate for caspases. GAS2L1 (also called GAS2-related protein on chromosome 22 or growth arrest-specific protein 2-like 1) and GAS2L2 (also called GAS2-related protein on chromosome 17 or growth arrest-specific protein 2-like 2) may be involved in the cross-linking of microtubules and microfilaments. GAS2L3, also called GAS2-like protein 3, is a cytoskeletal linker protein that may promote and stabilize the formation of the actin and microtubule network. Members of this family contain a single copy of the CH domain at the N-terminal region. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  20 KKYDEELEERLVEWIVMQCGPDVGrPDrgrlGFQVWLKNGVILSKLVNSL--------------YPEGSKPVKVPENPPS 85
Cdd:cd21204   1 EEALLPMKEDLAEWLNDLLGDDLT-PD----NFLDELRNGVVLCQLAQKIqeaaekareagkknGPPPSYKLKCNENAKP 75

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....
gi 13928744  86 MVFKQMEQVAQFLKAAEDYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGSLAVT 139
Cdd:cd21204  76 GSFFARDNVANFLRWCRKLGVDEVLLFESEDLVLHKNPRQVLLCLLELARIAAR 129

calponin homology (CH) domain found in the IQ motif containing GTPase activating protein family; Members of the IQ motif containing GTPase activating protein (IQGAP) family are associated with the Ras GTP-binding protein and act as essential regulators of cytoskeletal function. There are three known IQGAP family members: IQGAP1, IQGAP2, and IQGAP3. They are multi-domain molecules having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP1 negatively regulates Ras family GTPases by stimulating their intrinsic GTPase activity. It lacks GAP activity. Both IQGAP1 and IQGAP2 specifically bind to Cdc42 and Rac1, but not to RhoA. Despite similarities to part of the sequence of RasGAP, neither IQGAP1 nor IQGAP2 interacts with Ras. IQGAP3 regulates the organization of the cytoskeleton under the regulation of Rac1 and Cdc42 in neuronal cells. The depletion of IQGAP3 is shown to impair neurite or axon outgrowth in neuronal cells with disorganized cytoskeleton.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 13928744  56 LKNGVILSKLVNSLYPEGSKPVKVPENPPSmvFKQMEQVAQFLKAAEDYGVTKTDMFQTVDLFEGKDM 123
Cdd:cd21206  35 LRNGVVLAKLANKFAPKLVPLKKIYDVGLQ--FRHTDNINHFLRALKKIGLPKIFHFETTDLYEKKNI 100

calponin homology (CH) domain found in VAV proteins; VAV proteins function both as cytoplasmic guanine nucleotide exchange factors (GEFs) for Rho GTPases and as scaffold proteins, and they play important roles in cell signaling by coupling cell surface receptors to various effector functions. They play key roles in processes that require cytoskeletal reorganization including immune synapse formation, phagocytosis, cell spreading, and platelet aggregation, among others. Vertebrates have three VAV proteins (VAV1, VAV2, and VAV3). VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. This model corresponds to the CH domain, an actin-binding domain which is present as a single copy in VAV proteins.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 13928744  56 LKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAAED-YGVTKTDMFQTVDLFEGKDMAAVQRT 129
Cdd:cd21201  36 LRDGVLLCQLLNRLSPGSVDDREINLRPQMSQFLCLKNIRTFLQACRTvFGLRSADLFEPEDLYDVTNFGKVIRT 110

calponin homology (CH) domain found in LIM domain only protein 7 and similar proteins; This family includes LIM domain only protein 7 (LMO-7) and LIM and calponin homology domains-containing protein 1 (LIMCH1), and similar proteins. LMO-7, also called F-box only protein 20, or LOMP, is a transcription regulator for expression of many Emery-Dreifuss muscular dystrophy (EDMD)-relevant genes. It binds to alpha-actinin and AF6/afadin at adherens junctions for epithelial cell-cell adhesion. LIMCH1 acts as an actin stress fiber-associated protein that activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. It positively regulates actin stress fiber assembly and stabilizes focal adhesions, and therefore negatively regulates cell spreading and cell migration. Members of this family contain a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 13928744  52 FQVWLKNGVILSKLVNSLYPEGSKPVKVPENPpsmvFKQMEQVAQFLKAAEDYGVTKTDMFQTVDLFE 119
Cdd:cd21208  22 FRESLEDGILLCELINAIKPGSIKKINRLPTP----IAGLDNLNLFLKACEDLGLKDSQLFDPTDLQD 85

calponin homology (CH) domain found in VAV3 protein and similar proteins; VAV3 is ubiquitously expressed and functions as a phosphorylation-dependent guanine nucleotide exchange factor (GEF) for RhoA, RhoG, and Rac1. Its function has been implicated in the hematopoietic, bone, cerebellar, and cardiovascular systems. VAV3 is essential in axon guidance in neurons that control blood pressure and respiration. It is overexpressed in prostate cancer cells and it plays a role in regulating androgen receptor transcriptional activity. VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. The model corresponds to CH domain, an actin-binding domain which is present as a single copy in VAV3 protein.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 13928744  56 LKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAA-EDYGVTKTDMFQTVDLFEGKDMAAVQRTVMAL 133
Cdd:cd21264  36 LRDGVLLCQLLNNLRPHSINLKEINLRPQMSQFLCLKNIRTFLSACcETFGMRKSELFEAFDLFDVRDFGKVIETLSKL 114

calponin homology (CH) domain found in the Pak Interactive eXchange factor family; Pak Interactive eXchange factor (PIX) proteins are Rho guanine nucleotide exchange factors (GEFs), which activate small GTPases by exchanging bound GDP for free GTP. They act as GEFs for both Cdc42 and Rac1, and have been implicated in cell motility, adhesion, neurite outgrowth, and cell polarity. Vertebrates contain two proteins from the PIX family, alpha-PIX and beta-PIX. Alpha-PIX, also called Rho guanine nucleotide exchange factor 6 (ARHGEF6), is localized in dendritic spines where it regulates spine morphogenesis. It controls dendritic length and spine density in the hippocampus. Mutations in the ARHGEF6 gene cause X-linked intellectual disability in humans. Beta-PIX, also called Rho guanine nucleotide exchange factor 7 (ARHGEF7), plays important roles in regulating neuroendocrine exocytosis, focal adhesion maturation, cell migration, synaptic vesicle localization, and insulin secretion. Both alpha-PIX and beta-PIX contain a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  56 LKNGVILSKLVNSLYPEGSKpvKVPENPPSMVfKQMEQVAQFLKAAEDYGVTKTDMFQTVDLFEGKDMAAVQRTVMALGS 135
Cdd:cd21202  34 LKNGVVLCRLVNRLKPGTVE--KIYDEPTTEE-ECLYNFESFLKACQELGILAEEIFDPNDLYSGGNFQKVLSTLERLEK 110

                ..
gi 13928744 136 LA 137
Cdd:cd21202 111 VA 112

calponin homology (CH) domain found in leucine-rich repeat and calponin homology domain-containing protein 3; Leucine-rich repeat and calponin homology domain-containing protein 3 (LRCH3) is part of the DISP (DOCK7-Induced Septin disPlacement) complex. It may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. LRCH3 contains a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13928744  56 LKNGVILSKLVNSLYPEGSKPVKVPEN--PPSMVFKQMEQVAQFLKAAEDYGVTKTDMFQTVDLFEGKDMAAVQRTVMAL 133
Cdd:cd21272  26 LTDGVVLCHLANHVRPRSVPSIHVPSPavPKLTMAKCRRNVENFLEACRRIGVPQEQLCLPLHILEEKGLSQVAVTVQAL 105

                ....
gi 13928744 134 GSLA 137
Cdd:cd21272 106 LELA 109

calponin homology (CH) domain found in alpha-Pak Interactive eXchange factor; Alpha-Pak Interactive eXchange factor (alpha-PIX), also called PAK-interacting exchange factor alpha, Rho guanine nucleotide exchange factor 6 (ARHGEF6), Rac/Cdc42 guanine nucleotide exchange factor 6, or Cool (Cloned out of Library)-2, activates small GTPases by exchanging bound GDP for free GTP. It acts as a GEF for both Cdc42 and Rac1, and is localized in dendritic spines where it regulates spine morphogenesis. It controls dendritic length and spine density in the hippocampus. Mutations in the ARHGEF6 gene cause X-linked intellectual disability in humans. Alpha-PIX contains a single copy of the CH domain at its N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 13928744  56 LKNGVILSKLVNSLYPeGSKPVKVPEnpPSMVFKQMEQVAQFLKAAedyGVTKTDMFQTVDLFEGKDMAAVQRTVMAL 133
Cdd:cd21265  35 LKDGVVLCKLIERLLP-GSVEKYCLE--PKTEADCIGNIKEFLKGC---AALKVETFEPDDLYTGENFSKVLSTLLAV 106