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Conserved domains on  [gi|225735586|ref|NP_722470|]
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cytoplasmic tRNA 2-thiolation protein 2 [Mus musculus]

Protein Classification

cytoplasmic tRNA 2-thiolation protein 2( domain architecture ID 10563289)

cytoplasmic tRNA 2-thiolation protein 2 (CTU2) plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CTU2 pfam10288
Cytoplasmic tRNA 2-thiolation protein 2; CTU2 is a family of proteins necessary for the ...
 347-470 1.55e-27

Cytoplasmic tRNA 2-thiolation protein 2; CTU2 is a family of proteins necessary for the formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. The family is conserved from plants to humans ]1]. It plays a central role in the 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine, or the wobble nucleoside. This wobble modification in tRNAs, 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U), is required for the proper decoding of NNR codons in eukaryotes. The 2-thio group gives rigidity by largely fixing the C3'-endo ribose puckering, ensuring stable and accurate codon-anticodon pairing.


:

Pssm-ID: 463044  Cd Length: 106  Bit Score: 106.35  E-value: 1.55e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225735586  347 SIHRLMEAFILRLQTLFPSTVSTVYRTSEKLVKAPREgcaagPSGPSCLLCMCAL-DIDTADSATAFGAQSSSHLSQMPS 425
Cdd:pfam10288   1 SIQELTEKFITNLQADYPSTVSTVVRTGEKLVAPKIS-----DSSGKCSLCGSPLdDDPSEWLKTITVNEGSPLVSEEEK 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 225735586  426 AEAGMPTQPCCAAGEGQAQSCHrevgkrgdaracitEQLCYSCRV 470
Cdd:pfam10288  76 ELLEKWRESNLESLSCERLESG--------------KQLCYGCRV 106
TilS super family cl43000
tRNA(Ile)-lysidine synthase TilS/MesJ [Translation, ribosomal structure and biogenesis]; tRNA ...
 250-380 8.53e-03

tRNA(Ile)-lysidine synthase TilS/MesJ [Translation, ribosomal structure and biogenesis]; tRNA(Ile)-lysidine synthase TilS/MesJ is part of the Pathway/BioSystem: tRNA modification


The actual alignment was detected with superfamily member COG0037:

Pssm-ID: 439807 [Multi-domain]  Cd Length: 235  Bit Score: 37.89  E-value: 8.53e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225735586 250 TLRTHLIVHIARVHGYCKVMTG-------ETctrlaikLMTNLAlgRGAFLAWDTGFSDERHGDVVLVRPMRDHTLKEVA 322
Cdd:COG0037  101 RARYGALYELARELGADKIATGhhlddqaET-------FLLNLL--RGSGLAGLAGMPPSRGGGVRLIRPLLYVSRKEIE 171

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 225735586 323 FY---NHLfrvpsvftPAIDTKAPEKASIHR--LMEAFILRLQTLFPSTVSTVYRTSEKLVKA 380
Cdd:COG0037  172 AYakeNGL--------PWIEDPCNYDPRYTRnrIRHLVLPELEERNPGFKENLARSAENLAEE 226
 
Name Accession Description Interval E-value
CTU2 pfam10288
Cytoplasmic tRNA 2-thiolation protein 2; CTU2 is a family of proteins necessary for the ...
 347-470 1.55e-27

Cytoplasmic tRNA 2-thiolation protein 2; CTU2 is a family of proteins necessary for the formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. The family is conserved from plants to humans ]1]. It plays a central role in the 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine, or the wobble nucleoside. This wobble modification in tRNAs, 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U), is required for the proper decoding of NNR codons in eukaryotes. The 2-thio group gives rigidity by largely fixing the C3'-endo ribose puckering, ensuring stable and accurate codon-anticodon pairing.


Pssm-ID: 463044  Cd Length: 106  Bit Score: 106.35  E-value: 1.55e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225735586  347 SIHRLMEAFILRLQTLFPSTVSTVYRTSEKLVKAPREgcaagPSGPSCLLCMCAL-DIDTADSATAFGAQSSSHLSQMPS 425
Cdd:pfam10288   1 SIQELTEKFITNLQADYPSTVSTVVRTGEKLVAPKIS-----DSSGKCSLCGSPLdDDPSEWLKTITVNEGSPLVSEEEK 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 225735586  426 AEAGMPTQPCCAAGEGQAQSCHrevgkrgdaracitEQLCYSCRV 470
Cdd:pfam10288  76 ELLEKWRESNLESLSCERLESG--------------KQLCYGCRV 106
TilS COG0037
tRNA(Ile)-lysidine synthase TilS/MesJ [Translation, ribosomal structure and biogenesis]; tRNA ...
 250-380 8.53e-03

tRNA(Ile)-lysidine synthase TilS/MesJ [Translation, ribosomal structure and biogenesis]; tRNA(Ile)-lysidine synthase TilS/MesJ is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439807 [Multi-domain]  Cd Length: 235  Bit Score: 37.89  E-value: 8.53e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225735586 250 TLRTHLIVHIARVHGYCKVMTG-------ETctrlaikLMTNLAlgRGAFLAWDTGFSDERHGDVVLVRPMRDHTLKEVA 322
Cdd:COG0037  101 RARYGALYELARELGADKIATGhhlddqaET-------FLLNLL--RGSGLAGLAGMPPSRGGGVRLIRPLLYVSRKEIE 171

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 225735586 323 FY---NHLfrvpsvftPAIDTKAPEKASIHR--LMEAFILRLQTLFPSTVSTVYRTSEKLVKA 380
Cdd:COG0037  172 AYakeNGL--------PWIEDPCNYDPRYTRnrIRHLVLPELEERNPGFKENLARSAENLAEE 226
 
Name Accession Description Interval E-value
CTU2 pfam10288
Cytoplasmic tRNA 2-thiolation protein 2; CTU2 is a family of proteins necessary for the ...
 347-470 1.55e-27

Cytoplasmic tRNA 2-thiolation protein 2; CTU2 is a family of proteins necessary for the formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. The family is conserved from plants to humans ]1]. It plays a central role in the 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine, or the wobble nucleoside. This wobble modification in tRNAs, 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U), is required for the proper decoding of NNR codons in eukaryotes. The 2-thio group gives rigidity by largely fixing the C3'-endo ribose puckering, ensuring stable and accurate codon-anticodon pairing.


Pssm-ID: 463044  Cd Length: 106  Bit Score: 106.35  E-value: 1.55e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225735586  347 SIHRLMEAFILRLQTLFPSTVSTVYRTSEKLVKAPREgcaagPSGPSCLLCMCAL-DIDTADSATAFGAQSSSHLSQMPS 425
Cdd:pfam10288   1 SIQELTEKFITNLQADYPSTVSTVVRTGEKLVAPKIS-----DSSGKCSLCGSPLdDDPSEWLKTITVNEGSPLVSEEEK 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 225735586  426 AEAGMPTQPCCAAGEGQAQSCHrevgkrgdaracitEQLCYSCRV 470
Cdd:pfam10288  76 ELLEKWRESNLESLSCERLESG--------------KQLCYGCRV 106
TilS COG0037
tRNA(Ile)-lysidine synthase TilS/MesJ [Translation, ribosomal structure and biogenesis]; tRNA ...
 250-380 8.53e-03

tRNA(Ile)-lysidine synthase TilS/MesJ [Translation, ribosomal structure and biogenesis]; tRNA(Ile)-lysidine synthase TilS/MesJ is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439807 [Multi-domain]  Cd Length: 235  Bit Score: 37.89  E-value: 8.53e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225735586 250 TLRTHLIVHIARVHGYCKVMTG-------ETctrlaikLMTNLAlgRGAFLAWDTGFSDERHGDVVLVRPMRDHTLKEVA 322
Cdd:COG0037  101 RARYGALYELARELGADKIATGhhlddqaET-------FLLNLL--RGSGLAGLAGMPPSRGGGVRLIRPLLYVSRKEIE 171

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 225735586 323 FY---NHLfrvpsvftPAIDTKAPEKASIHR--LMEAFILRLQTLFPSTVSTVYRTSEKLVKA 380
Cdd:COG0037  172 AYakeNGL--------PWIEDPCNYDPRYTRnrIRHLVLPELEERNPGFKENLARSAENLAEE 226
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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