NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|568952696|ref|XP_006508576|]
View 

solute carrier family 22 member 18 isoform X3 [Mus musculus]

Protein Classification

MFS transporter( domain architecture ID 999995)

major facilitator superfamily (MFS) transporter facilitates the transport across cytoplasmic or internal membranes of one or more from a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085
PubMed:  26758938|26098515
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

Name Accession Description Interval E-value
MFS super family cl28910
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
1-279 7.07e-84

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


The actual alignment was detected with superfamily member cd17331:

Pssm-ID: 452895 [Multi-domain]  Cd Length: 382  Bit Score: 257.15  E-value: 7.07e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   1 MHTLPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEA 80
Cdd:cd17331  101 MHAMQAAQMVVTDLTDPGERAAALGRLGLSYGIGMVVGSFLGGLLTKKFGEQAAALVAAVGSLLSIVLVILLIPSNTKSK 180
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  81 SVQSAPQGGTKASVFDLKAITRLLLLPRVLPVFLVKVISGLPSGpgitaglllypgLFLVMFSIISMDFFQLEAAQAGYL 160
Cdd:cd17331  181 REKEAKESASPPSVFNLKEILRLLRLPGVAFLLLVKLLSGLPIG------------MFQSMFSLVAMDFFGLTPEENGYL 248
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 161 MSFFGILQMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLVFSLCTLNVVTDSMLTKAVSA 240
Cdd:cd17331  249 MSYAGVLGMVVQGLLVGPLTKRFGERSLLKLSVALLAIGYLLLAFVTTVWQLLVLLLPLVIGGSVLYTVITSLLTKAVPP 328
                        250       260       270
                 ....*....|....*....|....*....|....*....
gi 568952696 241 SDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYGVPIFGH 279
Cdd:cd17331  329 EDTGTALGLSHALFSLVRTVSPTIGGALLQAYGFPSFGL 367
 
Name Accession Description Interval E-value
MFS_SLC22A18 cd17331
Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; ...
1-279 7.07e-84

Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; Solute carrier family 22 member 18 (SLC22A18) is also called Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene A protein (BWR1A or BWSCR1A), efflux transporter-like protein, imprinted multi-membrane-spanning polyspecific transporter-related protein 1 (IMPT1), organic cation transporter-like protein 2 (ORCTL2), or tumor-suppressing subchromosomal transferable fragment candidate gene 5 protein (TSSC5). It is localized at the apical membrane surface of renal proximal tubules and may act as an organic cation/proton antiporter. It functions as a tumor suppressor in several cancer types including glioblastoma and colorectal cancer. SLC22A18 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340889 [Multi-domain]  Cd Length: 382  Bit Score: 257.15  E-value: 7.07e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   1 MHTLPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEA 80
Cdd:cd17331  101 MHAMQAAQMVVTDLTDPGERAAALGRLGLSYGIGMVVGSFLGGLLTKKFGEQAAALVAAVGSLLSIVLVILLIPSNTKSK 180
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  81 SVQSAPQGGTKASVFDLKAITRLLLLPRVLPVFLVKVISGLPSGpgitaglllypgLFLVMFSIISMDFFQLEAAQAGYL 160
Cdd:cd17331  181 REKEAKESASPPSVFNLKEILRLLRLPGVAFLLLVKLLSGLPIG------------MFQSMFSLVAMDFFGLTPEENGYL 248
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 161 MSFFGILQMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLVFSLCTLNVVTDSMLTKAVSA 240
Cdd:cd17331  249 MSYAGVLGMVVQGLLVGPLTKRFGERSLLKLSVALLAIGYLLLAFVTTVWQLLVLLLPLVIGGSVLYTVITSLLTKAVPP 328
                        250       260       270
                 ....*....|....*....|....*....|....*....
gi 568952696 241 SDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYGVPIFGH 279
Cdd:cd17331  329 EDTGTALGLSHALFSLVRTVSPTIGGALLQAYGFPSFGL 367
MFS_1 pfam07690
Major Facilitator Superfamily;
4-262 2.20e-08

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 54.35  E-value: 2.20e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696    4 LPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEASVQ 83
Cdd:pfam07690 103 FPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLLASLFGWRAAFLILAILSLLAAVLLLLPRPPPESKRPKP 182
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   84 SAPQGGTKASVFdlkaitrllllprvlpvflvKVISGLPSGPGITAGLLLYPGLFLVMFSI-ISMDFFQLEAAQAGYLMS 162
Cdd:pfam07690 183 AEEARLSLIVAW--------------------KALLRDPVLWLLLALLLFGFAFFGLLTYLpLYQEVLGLSALLAGLLLG 242
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  163 FFGILqMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLV---FSLCTLNVVTDSMLTKAVS 239
Cdd:pfam07690 243 LGGLL-GAIGRLLLGRLSDRLGRRRRLLLALLLLILAALGLLLLSLTLSSLWLLLALLllgFGFGLVFPALNALVSDLAP 321
                         250       260
                  ....*....|....*....|...
gi 568952696  240 ASDTGTMLGLSASVQPLTRTLGP 262
Cdd:pfam07690 322 KEERGTASGLYNTAGSLGGALGP 344
 
Name Accession Description Interval E-value
MFS_SLC22A18 cd17331
Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; ...
1-279 7.07e-84

Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; Solute carrier family 22 member 18 (SLC22A18) is also called Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene A protein (BWR1A or BWSCR1A), efflux transporter-like protein, imprinted multi-membrane-spanning polyspecific transporter-related protein 1 (IMPT1), organic cation transporter-like protein 2 (ORCTL2), or tumor-suppressing subchromosomal transferable fragment candidate gene 5 protein (TSSC5). It is localized at the apical membrane surface of renal proximal tubules and may act as an organic cation/proton antiporter. It functions as a tumor suppressor in several cancer types including glioblastoma and colorectal cancer. SLC22A18 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340889 [Multi-domain]  Cd Length: 382  Bit Score: 257.15  E-value: 7.07e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   1 MHTLPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEA 80
Cdd:cd17331  101 MHAMQAAQMVVTDLTDPGERAAALGRLGLSYGIGMVVGSFLGGLLTKKFGEQAAALVAAVGSLLSIVLVILLIPSNTKSK 180
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  81 SVQSAPQGGTKASVFDLKAITRLLLLPRVLPVFLVKVISGLPSGpgitaglllypgLFLVMFSIISMDFFQLEAAQAGYL 160
Cdd:cd17331  181 REKEAKESASPPSVFNLKEILRLLRLPGVAFLLLVKLLSGLPIG------------MFQSMFSLVAMDFFGLTPEENGYL 248
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 161 MSFFGILQMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLVFSLCTLNVVTDSMLTKAVSA 240
Cdd:cd17331  249 MSYAGVLGMVVQGLLVGPLTKRFGERSLLKLSVALLAIGYLLLAFVTTVWQLLVLLLPLVIGGSVLYTVITSLLTKAVPP 328
                        250       260       270
                 ....*....|....*....|....*....|....*....
gi 568952696 241 SDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYGVPIFGH 279
Cdd:cd17331  329 EDTGTALGLSHALFSLVRTVSPTIGGALLQAYGFPSFGL 367
MFS_SLC46_TetA_like cd17330
Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and ...
1-273 2.37e-32

Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of the eukaryotic proteins MFSD9, MFSD10, MFSD14, and SLC46 family proteins, as well as bacterial multidrug resistance (MDR) transporters such as tetracycline resistance protein TetA and multidrug resistance protein MdtG. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. TetA proteins confer resistance to tetracycline while MdtG confers resistance to fosfomycin and deoxycholate. The Solute carrier 46 (SLC46) family is composed of three vertebrate members (SLC46A1, SLC46A2, and SLC46A3), the best-studied of which is SLC46A1, which functions both as an intestinal proton-coupled high-affinity folate transporter involved in the absorption of folates and as an intestinal heme transporter which mediates heme uptake. MFSD10 facilitates the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs) and confers resistance to such NSAIDs. The SLC46/TetA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340888 [Multi-domain]  Cd Length: 349  Bit Score: 122.30  E-value: 2.37e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   1 MHTLPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPattkea 80
Cdd:cd17330  101 GGNVSVAQAYIADITDEEERAKAFGIISAAFGLGFILGPALGGFLSTPYGYALPFLVAAALALLNLVLVLFFLP------ 174
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  81 svqsapqggtkasvfdlkaitrllllpRVLPVFLVKVISGLPSGpgitaglllypgLFLVMFSIISMDFFQLEAAQAGYL 160
Cdd:cd17330  175 ---------------------------PLRRLLLVYFLIALAFA------------GLESVFSLFLKDRFGFSPSQIGLL 215
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 161 MSFFGILQMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLVFSLCTLNVVTDSMLTKAVSA 240
Cdd:cd17330  216 LALVGIAGILAQLLLIPRLAKRLGERKLLRLGLLLAIIGLLLLPFASSVPLLVLAAAILAFGTGIVFPALSALLSKSVPS 295
                        250       260       270
                 ....*....|....*....|....*....|...
gi 568952696 241 SDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYG 273
Cdd:cd17330  296 KEQGKAQGIIQSLQSLARIIGPLLAGPLYSFLG 328
MFS_MFSD9 cd17390
Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily ...
2-269 2.73e-21

Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily domain-containing protein 9 (MFSD9) is expressed in the central nervous system (CNS) and in most peripheral tissues but at very low expression levels. The function of MFSD9 is unknown. MFSD9 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340948 [Multi-domain]  Cd Length: 350  Bit Score: 92.29  E-value: 2.73e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   2 HTLPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTL-NTAYGIQCPAILAFVVTLLGAVLSFTCVPattkea 80
Cdd:cd17390   99 HTQSLCKAYLSDLVSEQERSRVLGRFNAASSLGFILGPVVGGHLaETDGGFYYVALLCSAIFILNAGLVWFLLS------ 172
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  81 svqsapqggtkaSVFDLkaitrllllprvlpvFLVKVISGLpsgpgitAGLLLYPglflvMFSIISMDFFQLEAAQAGYL 160
Cdd:cd17390  173 ------------KLWDL---------------LLVRFLMGF-------SVLLFRS-----NFSLYLEERFDLSPKTIGYL 213
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 161 MSFFGILQMmIQGLVIGRLSTHFPEE---ALLRSSVLVFAVVgLGMALMSSVLHFCFLMPGLVFSLCTLNVVTDSMLTKA 237
Cdd:cd17390  214 ISYSGVLGA-LSGFLVGRILKLYKNNeylLLLHSFILLALSL-LGLALAPSIWLLVLCLTPLSISSAVLRTCSTSLLLSR 291
                        250       260       270
                 ....*....|....*....|....*....|..
gi 568952696 238 VSASDTGTMLGLSASVQPLTRTLGPTLGGLLY 269
Cdd:cd17390  292 GKSKERGTLLGVGQSVTSLARILAPLLSGLLQ 323
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
3-275 8.18e-17

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 79.93  E-value: 8.18e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   3 TLPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEASV 82
Cdd:cd17325  101 VWPAAMALIADIAPPEERGRAMGIFSSAIGLGFLIGPLLGGLLADALGYRAPFLVCAALALLALVLALLLLPEPRPPPNK 180
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  83 QSAPQGGTKASVFDLkaitrlLLLPRVLPVFLVKVISGLPSgpgitaglllypGLFLVMFSIISMDFFQLEAAQAGYLMS 162
Cdd:cd17325  181 RAVSAARLRSGLRLL------LRDRRLLALFLAIFVLAFAF------------GALEPFLPLYAAELGGLSPAQIGLLFG 242
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 163 FFGILQMMIQGLViGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLVFSLCTLNVVTDSMLTKAVSASD 242
Cdd:cd17325  243 AQGLASALSQPPA-GKLSDRIGRKPLILIGLLLSAVALLLLPLATSFWLLLLLLALLGLGLGLVFPATLALLADIVPPEG 321
                        250       260       270
                 ....*....|....*....|....*....|...
gi 568952696 243 TGTMLGLSASVQPLTRTLGPTLGGLLYRSYGVP 275
Cdd:cd17325  322 RGTAMGLFNTAFSLGMVLGPLLGGFLYDAFGFA 354
MFS_TetA cd17388
Tetracycline resistance protein TetA and related proteins of the Major Facilitator Superfamily ...
7-269 5.55e-12

Tetracycline resistance protein TetA and related proteins of the Major Facilitator Superfamily of transporters; This subfamily is composed of tetracycline resistance proteins similar to Escherichia coli TetA(A), TetA(B), and TetA(E), which are metal-tetracycline/H(+) antiporters that confer resistance to tetracycline by an active tetracycline efflux, which is an energy-dependent process that decreases the accumulation of the antibiotic in cells. TetA-like tetracycline resistance proteins belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340946 [Multi-domain]  Cd Length: 385  Bit Score: 65.73  E-value: 5.55e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   7 AQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNtAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEASVQSAP 86
Cdd:cd17388  111 AAAYIADVTPPEQRARNFGLLGAAFGLGFIAGPALGGLLG-EISPRAPFLVAAGLNGLNFLLGLFVLPESLPPERRRAFR 189
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  87 QGGTKAsvfdlkaitrlllLPRVLPVFLVKVISGLpsgpgITAGLLLYPGLFLVM--FSIISMDFFQLEAAQAGYLMSFF 164
Cdd:cd17388  190 WRRANP-------------FGSLRWLTGFPGLLGL-----LAVFFLIQLIGQVPAttWVLFTEDRFDWSPTMVGLSLAVF 251
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 165 GILQMMIQGLVIGRLSTHFPEEallRSSVLVFAVVGLGMALMSSVLHFCFLMPGLVFsLCTLNVVT---DSMLTKAVSAS 241
Cdd:cd17388  252 GLLHALFQAFVTGPIAKRLGEK---RAILLGLAADALGFLLLAFATQGWMVFPILIL-LALGGIAGpalQGLLSRQVPEN 327
                        250       260
                 ....*....|....*....|....*...
gi 568952696 242 DTGTMLGLSASVQPLTRTLGPTLGGLLY 269
Cdd:cd17388  328 EQGELQGVLTSLTSLTSIIGPLLFTGIF 355
MFS_MFSD10 cd17389
Major facilitator superfamily domain-containing protein 10; Major facilitator superfamily ...
10-273 1.71e-08

Major facilitator superfamily domain-containing protein 10; Major facilitator superfamily domain-containing protein 10 (MFSD10) is also called tetracycline transporter-like protein (TETRAN). It is expressed in various human tissues, including the kidney. In cultured cells, its overexpression facilitated the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs). MFSD10/TETRAN overexpression cause resistance to some NSAIDs, suggesting that it may be an organic anion transporter that serves as an efflux pump for some NSAIDs and various other organic anions at the final excretion step. MFSD10 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340947 [Multi-domain]  Cd Length: 391  Bit Score: 54.97  E-value: 1.71e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  10 VITDLTAPTERPAALGRLGLCFGIGIIFGSLLGG---TLNTAYGI---QCPAILAFVVTLLGAVLSFTCVPATtkeasvq 83
Cdd:cd17389  140 IVADVTSPKKRSKGMALIGVAFSLGFIIGPMIGAyfaSFTRSLGEvffSMPALFALVLALADLLFIFFFLPET------- 212
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  84 sapqggtkasvfdlkaitrllllprvlpvflvkvisgLPSGPGITAG-----LLLYPGL-FLVMFsiISMDFFQLEAAQA 157
Cdd:cd17389  213 -------------------------------------LPSNLRRLGLvyflyLFLFSGLeFTLTF--LTHQRFQYTSMQQ 253
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 158 GYLMSFFGILQMMIQGLVIGRLSTHFPEEALLRSSVLVF-AVVGLGMALMSSVLHFCFLMpglvFSLCTLNVVT--DSML 234
Cdd:cd17389  254 GKMFFFIGIIMALVQGGYVRRIKPGKEKKAVLQGLILLIpAFLLIGWASSVTVLYAGLFL----YSFAAATVVPclTTLV 329
                        250       260       270
                 ....*....|....*....|....*....|....*....
gi 568952696 235 TKAVSASDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYG 273
Cdd:cd17389  330 SGYGSDDQKGTVMGIFRSLGALARALGPIVACTVYWLAG 368
MFS_1 pfam07690
Major Facilitator Superfamily;
4-262 2.20e-08

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 54.35  E-value: 2.20e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696    4 LPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEASVQ 83
Cdd:pfam07690 103 FPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLLASLFGWRAAFLILAILSLLAAVLLLLPRPPPESKRPKP 182
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   84 SAPQGGTKASVFdlkaitrllllprvlpvflvKVISGLPSGPGITAGLLLYPGLFLVMFSI-ISMDFFQLEAAQAGYLMS 162
Cdd:pfam07690 183 AEEARLSLIVAW--------------------KALLRDPVLWLLLALLLFGFAFFGLLTYLpLYQEVLGLSALLAGLLLG 242
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  163 FFGILqMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLV---FSLCTLNVVTDSMLTKAVS 239
Cdd:pfam07690 243 LGGLL-GAIGRLLLGRLSDRLGRRRRLLLALLLLILAALGLLLLSLTLSSLWLLLALLllgFGFGLVFPALNALVSDLAP 321
                         250       260
                  ....*....|....*....|...
gi 568952696  240 ASDTGTMLGLSASVQPLTRTLGP 262
Cdd:pfam07690 322 KEERGTASGLYNTAGSLGGALGP 344
MFS_MefA_like cd06173
Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of ...
5-276 6.57e-08

Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of Streptococcus pyogenes macrolide efflux protein A (MefA) and similar transporters, many of which remain uncharacterized. Some members may be multidrug resistance (MDR) transporters, which are drug/H+ antiporters (DHAs) that mediate the efflux of a variety of drugs and toxic compounds, conferring resistance to these compounds. MefA confers resistance to 14-membered macrolides including erythromycin and to 15-membered macrolides. It functions as an efflux pump to regulate intracellular macrolide levels. The MefA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340863 [Multi-domain]  Cd Length: 383  Bit Score: 53.39  E-value: 6.57e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   5 PAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEASVQS 84
Cdd:cd06173  111 PARQALLPELVPKEQLVRANALNSLATQLARIIGPALGGLLVALLGPGGAFAINALSFLLSALLLLFIRRPPPAAPGESS 190
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  85 APQGGTKASVFdlkaitrllllprvlpvFLVKVISGLPSGPGITAGLLLYPGLFLVMFSIISMDFFQLEAAQAGYLMSFF 164
Cdd:cd06173  191 SLLLRDLREGL-----------------RYLRRSPLLRLLLLALALFALLGGALTVLLPLLAKEVLGGGAAGYGLLLAAF 253
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 165 GIlQMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLVFSLCTLNVVTDSMLTKAVSASDTG 244
Cdd:cd06173  254 GV-GALLGALLLGRLSKRRRRGRLLLIGALLLGLALLVLGLSPSLWLLLAALFLLGLAGGLFNVPLNTLLQLRVPDELRG 332
                        250       260       270
                 ....*....|....*....|....*....|..
gi 568952696 245 TMLGLSASVQPLTRTLGPTLGGLLYRSYGVPI 276
Cdd:cd06173  333 RVFSVYNALNSGAMPLGALLAGLLADALGLSA 364
MFS_YfmO_like cd17474
Bacillus subtilis multidrug efflux protein YfmO and similar transporters of the Major ...
4-273 1.91e-05

Bacillus subtilis multidrug efflux protein YfmO and similar transporters of the Major Facilitator Superfamily; This family is composed of Bacillus subtilis multidrug efflux protein YfmO, bacillibactin exporter YmfD/YmfE, uncharacterized MFS-type transporter YvmA, and similar proteins. YfmO acts to efflux copper or a copper complex, and could contribute to copper resistance. YmfD/YmfE is involved in secretion of bacillibactin. The YfmO-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341027 [Multi-domain]  Cd Length: 374  Bit Score: 45.64  E-value: 1.91e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   4 LPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLnTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEASVQ 83
Cdd:cd17474  105 GPLALTLIGDLFEGGERAKAMGLYEAALGLGIAVGPLLGGLL-AAISWRYPFFLLAALIPVAFLAILFLLPEPKEKAKSS 183
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  84 SAPQGGTKASVFDLKAitrllllprvlpvFLVKVISGLpsgpgitAGLLLYpGLFLVMFSIISMDFFQLEAAQAGYLMSF 163
Cdd:cd17474  184 SATKLRDLKKALRGRG-------------LLTFLVSAF-------LYFFLY-FAFLTYLPFLLELLYGISPIIIGLLFAG 242
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 164 FGILqMMIQGLVIGRLSTH-FPEEALLRSSVLVFAVVGLGMALMSSVLhfCFLMPGLVFSLC--TLNVVTDSMLTKAVSA 240
Cdd:cd17474  243 WSVL-LALGSLFVGRLLKKrFGARRILVIGLLLYALSLLLLGFVPSLA--LLLIAIVLFGLGlgLNLPLLTTLVTELAPE 319
                        250       260       270
                 ....*....|....*....|....*....|...
gi 568952696 241 SDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYG 273
Cdd:cd17474  320 EERGTASSLYNFVRFLGQAVGPLLFGLLLEIFG 352
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
3-269 7.52e-05

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 43.95  E-value: 7.52e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   3 TLPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAF--VVTLLGAVLSFTCVPATTKEA 80
Cdd:cd06174  101 IDPAVLALIADLFPERERGRALGLLQAFGSVGGILGPLLGGILASSLGFGWRAVFLIaaALALLAAILLLLVVPDPPESA 180
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  81 SVQSAPQGGTKasvfdlkaitrllllpRVLPVFLVKVISGLPSGPGITAGLLLYPGLFLVMFSIISMDFFQLEAAQAGYL 160
Cdd:cd06174  181 RAKNEEASSKS----------------VLKLLKRVLKNPGLWLLLLAIFLVNLAYYSFSTLLPLFLLDLGGLSVAVAGLL 244
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 161 MSFFGILQMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLVFSLCTLNVVTDSMLTKAVSA 240
Cdd:cd06174  245 LSLFGLAGALGSLLLGLLSDRLIGRKPLLLIGLLLMALGLALLLLAPSLLLLLLLLLLLGFGLGGLLPLSFALIAELFPP 324
                        250       260
                 ....*....|....*....|....*....
gi 568952696 241 SDTGTMLGLSASVQPLTRTLGPTLGGLLY 269
Cdd:cd06174  325 EIRGTAFGLLNTFGFLGGAIGPLLAGFLL 353
MFS_MdtH_MDR_like cd17329
Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the ...
5-273 5.87e-04

Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of Escherichia coli MdtH and similar multidrug resistance (MDR) transporters from bacteria and archaea, many of which remain uncharacterized. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. MdtH confers resistance to norfloxacin and enoxacin. MdtH-like MDR transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340887 [Multi-domain]  Cd Length: 376  Bit Score: 41.06  E-value: 5.87e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   5 PAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLnTAYGIQCPAILAFVVTLLGAVLSFTCVPAT-TKEASVQ 83
Cdd:cd17329  107 PASEAMIADVTTPENRTRAFSLLYWAINLGVAIGPLLGGLL-LLYSFRLLFLAAAVLFLLAALVLLFLLPETrPKRRAAE 185
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  84 SAPQGGTKASVFDLKAITrllllprvlpvflvkvisglpsgpgitaglllypgLFLVMFSIISMDFFQLEAAQAGYLMSF 163
Cdd:cd17329  186 KTEILRGYKIVLRDRAFL-----------------------------------LFLLASFLISLIYFQLESTLPVYVVEI 230
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 164 FGILQMMIQGLV-------------IGRLSTHFPEEALLRSSVLVFAV----VGLGMALMSSVLHFCFLMPGLVFSLCTL 226
Cdd:cd17329  231 LGLGESLLGLLLalnallivllqfpVARLVERFRLRKILMIGILLFAIgfavLAFANTLAVLILAMIVLTLGEMLVFPAV 310
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
gi 568952696 227 NVVTDSMltkaVSASDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYG 273
Cdd:cd17329  311 QTLVAAL----APPDKRGSYMGFYALAWGLGRAIGPLVGGLLLDLLG 353
MFS_YfcJ_like cd17489
Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; ...
4-276 1.77e-03

Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of Escherichia coli membrane proteins, YfcJ and YhhS, Bacillus subtilis uncharacterized MFS-type transporter YwoG, and similar proteins. YfcJ and YhhS are putative arabinose efflux transporters. YhhS has been implicated glyphosate resistance. YfcJ-like arabinose efflux transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341042 [Multi-domain]  Cd Length: 367  Bit Score: 39.50  E-value: 1.77e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   4 LPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTcVPATTKEASVQ 83
Cdd:cd17489  102 TTAAATLVADIIPPSRRGEGIGYYGLATTLAMALGPALGLFLYQHLGFAVLFIVAAVLALLALLLVFL-VKDPPPVAAEE 180
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  84 SAPQGGTKASVFDLKAITrllllprvlpvflvkvisglpsgpgITAGLLLYPGLFLVMFSIISMDFFQLEAAQAGYLMSF 163
Cdd:cd17489  181 AEAGTRAFRRLVEKKVLP-------------------------PALILFLASIAYGAISTFLPLYAAERGISNAGLFFTV 235
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 164 FGILqMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHF--CFLMPGLVFSLctLNVVTDSMLTKAVSAS 241
Cdd:cd17489  236 YAIA-LLLSRPFSGKLSDRKGPKTVIIPGLLLLALGLLLLSFAGSPWMLllAAVLYGLGFGL--LFPALQALAVDLVPPH 312
                        250       260       270
                 ....*....|....*....|....*....|....*
gi 568952696 242 DTGTMLGLSASVQPLTRTLGPTLGGLLYRSYGVPI 276
Cdd:cd17489  313 RRGAANGTFFIFFDLGIGLGGLLLGLVAELFGYRA 347
MFS_1 pfam07690
Major Facilitator Superfamily;
136-273 3.24e-03

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 38.55  E-value: 3.24e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  136 GLFLVMFSIISMDFFQLEAAQAGYLMSFFGILQMMIQgLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFL 215
Cdd:pfam07690  13 SLLGPALPLLLAEDLGISPTEIGLLLTLFSLGYALAQ-PLAGRLSDRFGRRRVLLIGLLLFALGLLLLLFASSLWLLLVL 91
                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 568952696  216 MPGLVFSLCTLNVVTDSMLTKAVSASDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYG 273
Cdd:pfam07690  92 RVLQGLGAGALFPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLLASLFG 149
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
129-274 4.79e-03

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 38.18  E-value: 4.79e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 129 AGLLLYPGLFLVMFSIIS-MDFFQLEAAQAGYLMSFFGILQMMIQgLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMS 207
Cdd:cd06174    4 GFFLTGLARGLISPLLPAlLQSFGLSASQLGLLFALFSLGYALLQ-PLAGLLADRFGRRPVLLLGLLLFALGALLFAFAP 82
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 568952696 208 SVLHFCFLMPGLVFSLCTLNVVTDSMLTKAVSASDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYGV 274
Cdd:cd06174   83 SFWLLLLGRFLLGLGSGLIDPAVLALIADLFPERERGRALGLLQAFGSVGGILGPLLGGILASSLGF 149
MFS_MMR_MDR_like cd17504
Methylenomycin A resistance protein (also called MMR peptide)-like multidrug resistance (MDR) ...
1-277 4.88e-03

Methylenomycin A resistance protein (also called MMR peptide)-like multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This subfamily is composed of putative multidrug resistance (MDR) transporters including Chlamydia trachomatis antiseptic resistance protein QacA_2, and Serratia sp. DD3 Bmr3. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. This subfamily belongs to the Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance (MDR) transporters (MMR-like MDR transporter) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341047 [Multi-domain]  Cd Length: 371  Bit Score: 37.94  E-value: 4.88e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   1 MHTLPAAQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQcpaiLAFVVTLLGAVLSFTCVPATTKEA 80
Cdd:cd17504   98 GAVFPLAFALVREEFPPERVPVAQGILSATFGIGAGIGLPLGGWIVQHLGWR----WIFWTAAPVAVLLAALVPLIDLRL 173
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  81 SVQsapqggtkASVFDLKAITRLLLLPRVLPVFLVKVISGLPSGPGitaglllypglflvmfsiismdfFQLEAAQAGYL 160
Cdd:cd17504  174 LRR--------RNVLLANITALLAGFGMFMVFQLLPQLLQLPTPTG-----------------------FGLGITEAGLL 222
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 161 MSFFGILqMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCFLMPGLV---FSLCTLNVVTdsMLTKA 237
Cdd:cd17504  223 LLPGALV-MLIAGPLAGRLIARVGPRRVLLIGLVISAVGLLLLALLHSSLWALVVALLLIgigLGLAFASLPN--IVVES 299
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|
gi 568952696 238 VSASDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYGVPIF 277
Cdd:cd17504  300 VPPDRTGIATGMNTVLRTIGSAIGPAIAGAILTTYTVSIT 339
MFS_MdfA_MDR_like cd17320
Multidrug transporter MdfA and similar multidrug resistance (MDR) transporters of the Major ...
7-276 9.88e-03

Multidrug transporter MdfA and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as MdfA (also called chloramphenicol resistance pump Cmr), EmrD, MdtM, MdtL, bicyclomycin resistance protein (also called sulfonamide resistance protein), and the uncharacterized inner membrane transport protein YdhC. EmrD is a proton-dependent secondary transporter, first identified as an efflux pump for uncouplers of oxidative phosphorylation. It expels a range of drug molecules and amphipathic compounds across the inner membrane of E. coli. Similarly, MdfA is a secondary multidrug transporter that exports a broad spectrum of structurally and electrically dissimilar toxic compounds. These MDR transporters are drug/H+ antiporters (DHA) belonging to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340878 [Multi-domain]  Cd Length: 379  Bit Score: 37.17  E-value: 9.88e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696   7 AQMVITDLTAPTERPAALGRLGLCFGIGIIFGSLLGGTLNTAYGIQCPAILAFVVTLLGAVLSFTCVPATTKEASVQSAP 86
Cdd:cd17320  108 ARAIVRDLYSGAELAKIFSLLMMILAIAPALAPLLGGLLLLFFGWRAIFVFLALLGLLLLLLVLLFLPETLPEEKRKPLL 187
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696  87 QGgtkasvfdlkaitrllllprvlpvfLVKVISGLPSGPGITAGLLLYPGLFLVMFS------IISMDFFQLEAAQAGYL 160
Cdd:cd17320  188 SS-------------------------LLRAYKRVLKNRRFLGYALALGLSFAGLFAylaaapFIYIEQLGLSPAQFGLL 242
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568952696 161 MSFFGILqMMIQGLVIGRLSTHFPEEALLRSSVLVFAVVGLGMALMSSVLHFCF--LMPGLVFSLCTLNVVTDSMLTKAV 238
Cdd:cd17320  243 FALNAIA-LILGSLLNGRLVRRFGPRRLLRLGLLLLLAAALVLLLAALLGGLSLwpLVAPLFLIFFGFGLIFPNATSLAL 321
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|
gi 568952696 239 SA--SDTGTMLGLSASVQPLTRTLGPTLGGLLYRSYGVPI 276
Cdd:cd17320  322 EPfpHVAGTASALLGFLQFLIGALAGALVGLLPNGTALPM 361
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.20
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH