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Conserved domains on  [gi|568967752|ref|XP_006513802|]
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WASH complex subunit 4 isoform X5 [Mus musculus]

Protein Classification

WASH complex subunit 4 family protein( domain architecture ID 10384908)

WASH complex subunit 4 family protein is a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
WASH-7_mid pfam14744
WASH complex subunit 7; This family is the central, conserved region of proteins that form ...
193-537 0e+00

WASH complex subunit 7; This family is the central, conserved region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes.


:

Pssm-ID: 434177  Cd Length: 346  Bit Score: 628.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  193 FLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHSRHLES-HELLLDCYDKEIMDILNEHLLDKLCKEIEKD 271
Cdd:pfam14744   1 FLYWHRELLPLYLSDVYENPLQASRIQYLLSALSDCVPLLKSAKHLENpGKGLLDSYEEEIEKALKEEILEPLCRDIETD 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  272 LRLSVHTHLKLDDRNPFKVGRKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYG 351
Cdd:pfam14744  81 LRLHVHSHLLLDDRNPFKSGVKDLSRFLRLKPLRLFDKFIDIKAEVEHYLESTFYNLTTVALHDWKTYEEMRNLAKLKYG 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  352 LVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTY 431
Cdd:pfam14744 161 LNLLDPHLPGQTLEQGLDVLEIMRNIHVFVSRYNYNLNNQIFVERSSDSKHLNTIGIRHIANSIRTHGTGIMNTTVNFTY 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  432 QFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLGITPEGQSYLDQFRQLISQIGNAM 511
Cdd:pfam14744 241 QFLRKKFYIFSQFLFDDHIKSRLLKDLRFFRENKKELDGKYPYERAEKFIKGIRKLGLGDDGLSYLDQFRILITQIGNAL 320
                         330       340
                  ....*....|....*....|....*.
gi 568967752  512 GYIRMIRSGGLHCSSNAIRFVPDLED 537
Cdd:pfam14744 321 GYVRMLRSGGLHACSNAIRFVPDLEE 346
WASH-7_C pfam14746
WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins ...
554-723 2.43e-85

WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes. The C-terminus is predicted to include a transmembrane region.


:

Pssm-ID: 464293  Cd Length: 175  Bit Score: 267.58  E-value: 2.43e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  554 TLRAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKLGAAF 633
Cdd:pfam14746   1 TVKAARNLDKVISSLKKNFSEGTDYFKLLVDVFAPELRSPKNMHLRNFYLIVPALTLNYVEHMISAKEKLLKKNKVGASF 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  634 TDDGFAMGVAYTLKLLDQYQEFDSLHWFQSVREKYIKEIRAV-----AKQQNVQSTSQDEKLLQTMNLTQKRLEVYLQEF 708
Cdd:pfam14746  81 TDDGFAMGLAYILKLLDQYEEFDSLHWFESVKKKYNEELEDVqeilaAQSKQASAQKEDEKLQQTMHLTLKRLEAYLREF 160
                         170
                  ....*....|....*
gi 568967752  709 ELLYFSLSSARIFFR 723
Cdd:pfam14746 161 DLLFYSLSSARIFFR 175
WASH-4_N super family cl20671
WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins ...
1-188 2.73e-78

WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins that form subunit 4 of the WASH complex, also known as subunit 7 or KIAA1033/SWIP. In species such as Drosophila this protein is the only component of the 'complex'. This complex is present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. This domain comprises the WAHD1 and the TBR (tubulin-binding region) domains.


The actual alignment was detected with superfamily member pfam14745:

Pssm-ID: 434178  Cd Length: 566  Bit Score: 262.26  E-value: 2.73e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752    1 MMKMESMLSKEQRMDTFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLIELLKAIEHMFYR 80
Cdd:pfam14745 378 MVRMESILTKTVLANGFFEDLKSRCKLLIQGLLLASQISHLIKTVINLHVALGKPMTKSDVLLICKLIEMLKSIEHTFHR 457
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752   81 RSMVVADSVSHITQHLQHQALSSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALS-VGTQMKT 159
Cdd:pfam14745 458 RQILIVRSLSSLLQYLSYLILHLLETAKKRLQSDKKYSERKLDVLSALKLAEKLLNGPPTKERRLIIRLALSeVIQQNKS 537
                         170       180
                  ....*....|....*....|....*....
gi 568967752  160 FKDEELFPLQVVMKKLDLISELRERVQAQ 188
Cdd:pfam14745 538 FKEDELVKLRSILKRLDTISDLQENIQEQ 566
 
Name Accession Description Interval E-value
WASH-7_mid pfam14744
WASH complex subunit 7; This family is the central, conserved region of proteins that form ...
193-537 0e+00

WASH complex subunit 7; This family is the central, conserved region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes.


Pssm-ID: 434177  Cd Length: 346  Bit Score: 628.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  193 FLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHSRHLES-HELLLDCYDKEIMDILNEHLLDKLCKEIEKD 271
Cdd:pfam14744   1 FLYWHRELLPLYLSDVYENPLQASRIQYLLSALSDCVPLLKSAKHLENpGKGLLDSYEEEIEKALKEEILEPLCRDIETD 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  272 LRLSVHTHLKLDDRNPFKVGRKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYG 351
Cdd:pfam14744  81 LRLHVHSHLLLDDRNPFKSGVKDLSRFLRLKPLRLFDKFIDIKAEVEHYLESTFYNLTTVALHDWKTYEEMRNLAKLKYG 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  352 LVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTY 431
Cdd:pfam14744 161 LNLLDPHLPGQTLEQGLDVLEIMRNIHVFVSRYNYNLNNQIFVERSSDSKHLNTIGIRHIANSIRTHGTGIMNTTVNFTY 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  432 QFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLGITPEGQSYLDQFRQLISQIGNAM 511
Cdd:pfam14744 241 QFLRKKFYIFSQFLFDDHIKSRLLKDLRFFRENKKELDGKYPYERAEKFIKGIRKLGLGDDGLSYLDQFRILITQIGNAL 320
                         330       340
                  ....*....|....*....|....*.
gi 568967752  512 GYIRMIRSGGLHCSSNAIRFVPDLED 537
Cdd:pfam14744 321 GYVRMLRSGGLHACSNAIRFVPDLEE 346
WASH-7_C pfam14746
WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins ...
554-723 2.43e-85

WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes. The C-terminus is predicted to include a transmembrane region.


Pssm-ID: 464293  Cd Length: 175  Bit Score: 267.58  E-value: 2.43e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  554 TLRAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKLGAAF 633
Cdd:pfam14746   1 TVKAARNLDKVISSLKKNFSEGTDYFKLLVDVFAPELRSPKNMHLRNFYLIVPALTLNYVEHMISAKEKLLKKNKVGASF 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  634 TDDGFAMGVAYTLKLLDQYQEFDSLHWFQSVREKYIKEIRAV-----AKQQNVQSTSQDEKLLQTMNLTQKRLEVYLQEF 708
Cdd:pfam14746  81 TDDGFAMGLAYILKLLDQYEEFDSLHWFESVKKKYNEELEDVqeilaAQSKQASAQKEDEKLQQTMHLTLKRLEAYLREF 160
                         170
                  ....*....|....*
gi 568967752  709 ELLYFSLSSARIFFR 723
Cdd:pfam14746 161 DLLFYSLSSARIFFR 175
WASH-4_N pfam14745
WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins ...
1-188 2.73e-78

WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins that form subunit 4 of the WASH complex, also known as subunit 7 or KIAA1033/SWIP. In species such as Drosophila this protein is the only component of the 'complex'. This complex is present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. This domain comprises the WAHD1 and the TBR (tubulin-binding region) domains.


Pssm-ID: 434178  Cd Length: 566  Bit Score: 262.26  E-value: 2.73e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752    1 MMKMESMLSKEQRMDTFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLIELLKAIEHMFYR 80
Cdd:pfam14745 378 MVRMESILTKTVLANGFFEDLKSRCKLLIQGLLLASQISHLIKTVINLHVALGKPMTKSDVLLICKLIEMLKSIEHTFHR 457
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752   81 RSMVVADSVSHITQHLQHQALSSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALS-VGTQMKT 159
Cdd:pfam14745 458 RQILIVRSLSSLLQYLSYLILHLLETAKKRLQSDKKYSERKLDVLSALKLAEKLLNGPPTKERRLIIRLALSeVIQQNKS 537
                         170       180
                  ....*....|....*....|....*....
gi 568967752  160 FKDEELFPLQVVMKKLDLISELRERVQAQ 188
Cdd:pfam14745 538 FKEDELVKLRSILKRLDTISDLQENIQEQ 566
 
Name Accession Description Interval E-value
WASH-7_mid pfam14744
WASH complex subunit 7; This family is the central, conserved region of proteins that form ...
193-537 0e+00

WASH complex subunit 7; This family is the central, conserved region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes.


Pssm-ID: 434177  Cd Length: 346  Bit Score: 628.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  193 FLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHSRHLES-HELLLDCYDKEIMDILNEHLLDKLCKEIEKD 271
Cdd:pfam14744   1 FLYWHRELLPLYLSDVYENPLQASRIQYLLSALSDCVPLLKSAKHLENpGKGLLDSYEEEIEKALKEEILEPLCRDIETD 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  272 LRLSVHTHLKLDDRNPFKVGRKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYG 351
Cdd:pfam14744  81 LRLHVHSHLLLDDRNPFKSGVKDLSRFLRLKPLRLFDKFIDIKAEVEHYLESTFYNLTTVALHDWKTYEEMRNLAKLKYG 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  352 LVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTY 431
Cdd:pfam14744 161 LNLLDPHLPGQTLEQGLDVLEIMRNIHVFVSRYNYNLNNQIFVERSSDSKHLNTIGIRHIANSIRTHGTGIMNTTVNFTY 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  432 QFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLGITPEGQSYLDQFRQLISQIGNAM 511
Cdd:pfam14744 241 QFLRKKFYIFSQFLFDDHIKSRLLKDLRFFRENKKELDGKYPYERAEKFIKGIRKLGLGDDGLSYLDQFRILITQIGNAL 320
                         330       340
                  ....*....|....*....|....*.
gi 568967752  512 GYIRMIRSGGLHCSSNAIRFVPDLED 537
Cdd:pfam14744 321 GYVRMLRSGGLHACSNAIRFVPDLEE 346
WASH-7_C pfam14746
WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins ...
554-723 2.43e-85

WASH complex subunit 7, C-terminal; This family is the conserved C-terminal region of proteins that form subunit 7 of the WASH complex. In species such as Drosophila this protein is the only component of the 'complex'. This complex is a nucleation promoting factor necessary for the activation of Arp2/3 that nucleates and organizes actin filaments by associating with a pre-existing filament to induce the assembly of a branching filament. WASH thus effectively nucleates actin on endosomes. The C-terminus is predicted to include a transmembrane region.


Pssm-ID: 464293  Cd Length: 175  Bit Score: 267.58  E-value: 2.43e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  554 TLRAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKLGAAF 633
Cdd:pfam14746   1 TVKAARNLDKVISSLKKNFSEGTDYFKLLVDVFAPELRSPKNMHLRNFYLIVPALTLNYVEHMISAKEKLLKKNKVGASF 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752  634 TDDGFAMGVAYTLKLLDQYQEFDSLHWFQSVREKYIKEIRAV-----AKQQNVQSTSQDEKLLQTMNLTQKRLEVYLQEF 708
Cdd:pfam14746  81 TDDGFAMGLAYILKLLDQYEEFDSLHWFESVKKKYNEELEDVqeilaAQSKQASAQKEDEKLQQTMHLTLKRLEAYLREF 160
                         170
                  ....*....|....*
gi 568967752  709 ELLYFSLSSARIFFR 723
Cdd:pfam14746 161 DLLFYSLSSARIFFR 175
WASH-4_N pfam14745
WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins ...
1-188 2.73e-78

WASH complex subunit 4, N-terminal; This entry is the conserved N-terminal region of proteins that form subunit 4 of the WASH complex, also known as subunit 7 or KIAA1033/SWIP. In species such as Drosophila this protein is the only component of the 'complex'. This complex is present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. This domain comprises the WAHD1 and the TBR (tubulin-binding region) domains.


Pssm-ID: 434178  Cd Length: 566  Bit Score: 262.26  E-value: 2.73e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752    1 MMKMESMLSKEQRMDTFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLIELLKAIEHMFYR 80
Cdd:pfam14745 378 MVRMESILTKTVLANGFFEDLKSRCKLLIQGLLLASQISHLIKTVINLHVALGKPMTKSDVLLICKLIEMLKSIEHTFHR 457
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568967752   81 RSMVVADSVSHITQHLQHQALSSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALS-VGTQMKT 159
Cdd:pfam14745 458 RQILIVRSLSSLLQYLSYLILHLLETAKKRLQSDKKYSERKLDVLSALKLAEKLLNGPPTKERRLIIRLALSeVIQQNKS 537
                         170       180
                  ....*....|....*....|....*....
gi 568967752  160 FKDEELFPLQVVMKKLDLISELRERVQAQ 188
Cdd:pfam14745 538 FKEDELVKLRSILKRLDTISDLQENIQEQ 566
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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