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Conserved domains on  [gi|568976391|ref|XP_006534551|]
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protein spinster homolog 3 isoform X2 [Mus musculus]

Protein Classification

spinster family MFS transporter( domain architecture ID 13023647)

spinster family major facilitator superfamily (MFS) transporter may function as a sphingolipid transporter that plays a central role in endosome and/or lysosome storage

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085|GO:0006869
PubMed:  26758938|26098515
TCDB:  2.A.1.49

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS_spinster_like cd17328
Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters; ...
 16-257 1.13e-80

Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters; The protein spinster family includes Drosophila protein spinster, its vertebrate homologs, and similar proteins. Humans contain three homologs called protein spinster homologs 1 (SPNS1), 2 (SPNS2), and 3 (SPNS3). Protein spinster and its homologs may be sphingolipid transporters that play central roles in endosomes and/or lysosomes storage. SPNS2 is also called sphingosine 1-phosphate (S1P) transporter and is required for migration of myocardial precursors. S1P is a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. The spinster-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


:

Pssm-ID: 340886 [Multi-domain]  Cd Length: 405  Bit Score: 249.85  E-value: 1.13e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  16 VPDVPRGAAEkqGEVAVRAPRSSWCEDVRYLGRNWSFVFSTLGVTAIAFVTGALGFWAPKFLFEARVVHGLQLpcfqeqc 95
Cdd:cd17328  175 VREPPRGAAE--GGVMAKSTATSFLADLKALAKNPSFILSTLGFTAVSFVTGALAFWAPTFFLRAFGVGDLSS------- 245

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  96 hSQDSLIFGALTVATGIIGVMLGAEASRRYKKVNPRAEPLICASSLFATAPCLYLALILASRTLLASYVFLALGELLLSC 175
Cdd:cd17328  246 -ADVGLIFGAITCIAGVLGVLLGGILADRLRRKTPRADPLVCAVGLLAAAPFLFLALVLALSNLLLAWVLLFLGEFLLSL 324

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391 176 NWAVVADILLSVVVPRCRGTAEALQITVAHVLGDAGSPYLTGLISSVLqAERPDSYLQHFLSLQHSFLCCAFAIVLGGGF 255
Cdd:cd17328  325 NWAPVFAILLYVVIPTRRSTASAIQILLSHLLGDAGSPYLIGLISDLL-RQNKDSPLAEAASLQYALLLCSFVLVLGGFF 403


                 ..
gi 568976391 256 FL 257
Cdd:cd17328  404 FL 405
 
Name Accession Description Interval E-value
MFS_spinster_like cd17328
Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters; ...
 16-257 1.13e-80

Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters; The protein spinster family includes Drosophila protein spinster, its vertebrate homologs, and similar proteins. Humans contain three homologs called protein spinster homologs 1 (SPNS1), 2 (SPNS2), and 3 (SPNS3). Protein spinster and its homologs may be sphingolipid transporters that play central roles in endosomes and/or lysosomes storage. SPNS2 is also called sphingosine 1-phosphate (S1P) transporter and is required for migration of myocardial precursors. S1P is a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. The spinster-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340886 [Multi-domain]  Cd Length: 405  Bit Score: 249.85  E-value: 1.13e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  16 VPDVPRGAAEkqGEVAVRAPRSSWCEDVRYLGRNWSFVFSTLGVTAIAFVTGALGFWAPKFLFEARVVHGLQLpcfqeqc 95
Cdd:cd17328  175 VREPPRGAAE--GGVMAKSTATSFLADLKALAKNPSFILSTLGFTAVSFVTGALAFWAPTFFLRAFGVGDLSS------- 245

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  96 hSQDSLIFGALTVATGIIGVMLGAEASRRYKKVNPRAEPLICASSLFATAPCLYLALILASRTLLASYVFLALGELLLSC 175
Cdd:cd17328  246 -ADVGLIFGAITCIAGVLGVLLGGILADRLRRKTPRADPLVCAVGLLAAAPFLFLALVLALSNLLLAWVLLFLGEFLLSL 324

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391 176 NWAVVADILLSVVVPRCRGTAEALQITVAHVLGDAGSPYLTGLISSVLqAERPDSYLQHFLSLQHSFLCCAFAIVLGGGF 255
Cdd:cd17328  325 NWAPVFAILLYVVIPTRRSTASAIQILLSHLLGDAGSPYLIGLISDLL-RQNKDSPLAEAASLQYALLLCSFVLVLGGFF 403


                 ..
gi 568976391 256 FL 257
Cdd:cd17328  404 FL 405
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
50-265 1.47e-07

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 52.18  E-value: 1.47e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  50 WSFVFSTLGVTAIAFVTGALGFWAPKFLFEarvVHGLQLpcfqeqchsQDSLIFGALTVATGIIGVMLGAEASRRYKkvn 129
Cdd:COG2271  180 LRFWLLALAYFLVYFALYGFLTWLPTYLVE---VRGLSL---------AQAGLLLSLPFLAGIVGSLLGGWLSDRLG--- 244

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391 130 pRAEPLICASSLFATAPCLYLALILASRTLLasYVFLALGELLLSCNWAVVADILLSVVVPRCRGTAEALqITVAHVLGD 209
Cdd:COG2271  245 -RRRKLVLAIGLLLAALALLLLALLPSPALA--IALLFLAGFGLGGAFGLLWALAAELFPKKARGTASGL-VNTFGFLGG 320

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 568976391 210 AGSPYLTGLISSvlqaerpdsylqhFLSLQHSFLCCAFAIVLGGGFFLLTALHLEK 265
Cdd:COG2271  321 ALGPLLVGYLLD-------------ATGYQAAFLLLAALALLAALLALLLLRETRK 363
OATP pfam03137
Organic Anion Transporter Polypeptide (OATP) family; This family consists of several ...
 43-176 9.71e-03

Organic Anion Transporter Polypeptide (OATP) family; This family consists of several eukaryotic Organic-Anion-Transporting Polypeptides (OATPs). Several have been identified mostly in human and rat. Different OATPs vary in tissue distribution and substrate specificity. Since the numbering of different OATPs in particular species was based originally on the order of discovery, similarly numbered OATPs in humans and rats did not necessarily correspond in function, tissue distribution and substrate specificity (in spite of the name, some OATPs also transport organic cations and neutral molecules). Thus, Tamai et al. initiated the current scheme of using digits for rat OATPs and letters for human ones. Prostaglandin transporter (PGT) proteins are also considered to be OATP family members. In addition, the methotrexate transporter OATK is closely related to OATPs. This family also includes several predicted proteins from Caenorhabditis elegans and Drosophila melanogaster. This similarity was not previously noted. Note: Members of this family are described (in the Swiss-Prot database) as belonging to the SLC21 family of transporters.


Pssm-ID: 460821 [Multi-domain]  Cd Length: 526  Bit Score: 37.57  E-value: 9.71e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391   43 VRYLGRNWSFVFSTLGvtAIAFVTGALGFWA--PKFLfearvvhglqlpcfQEQCH---SQDSLIFGALTVATGIIGVML 117
Cdd:pfam03137 239 LKRLLKNPVYMLLTLA--SVFEFLLIAGYATflPKYI--------------ETQFGlsaSTANLLTGVVAIPGAALGILL 302

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 568976391  118 GAEASRRYkKVNPRAEP-LICASSLFATAPCLYLALI------LASRTLLASYVFLALGELLLSCN 176
Cdd:pfam03137 303 GGLIIKKF-KLSAKGLAkFNVITSLVSLLLSLLLFFLgcpnspVAGITVPYGGTQQLSGNLTSSCN 367
 
Name Accession Description Interval E-value
MFS_spinster_like cd17328
Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters; ...
 16-257 1.13e-80

Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters; The protein spinster family includes Drosophila protein spinster, its vertebrate homologs, and similar proteins. Humans contain three homologs called protein spinster homologs 1 (SPNS1), 2 (SPNS2), and 3 (SPNS3). Protein spinster and its homologs may be sphingolipid transporters that play central roles in endosomes and/or lysosomes storage. SPNS2 is also called sphingosine 1-phosphate (S1P) transporter and is required for migration of myocardial precursors. S1P is a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. The spinster-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340886 [Multi-domain]  Cd Length: 405  Bit Score: 249.85  E-value: 1.13e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  16 VPDVPRGAAEkqGEVAVRAPRSSWCEDVRYLGRNWSFVFSTLGVTAIAFVTGALGFWAPKFLFEARVVHGLQLpcfqeqc 95
Cdd:cd17328  175 VREPPRGAAE--GGVMAKSTATSFLADLKALAKNPSFILSTLGFTAVSFVTGALAFWAPTFFLRAFGVGDLSS------- 245

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  96 hSQDSLIFGALTVATGIIGVMLGAEASRRYKKVNPRAEPLICASSLFATAPCLYLALILASRTLLASYVFLALGELLLSC 175
Cdd:cd17328  246 -ADVGLIFGAITCIAGVLGVLLGGILADRLRRKTPRADPLVCAVGLLAAAPFLFLALVLALSNLLLAWVLLFLGEFLLSL 324

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391 176 NWAVVADILLSVVVPRCRGTAEALQITVAHVLGDAGSPYLTGLISSVLqAERPDSYLQHFLSLQHSFLCCAFAIVLGGGF 255
Cdd:cd17328  325 NWAPVFAILLYVVIPTRRSTASAIQILLSHLLGDAGSPYLIGLISDLL-RQNKDSPLAEAASLQYALLLCSFVLVLGGFF 403


                 ..
gi 568976391 256 FL 257
Cdd:cd17328  404 FL 405
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
50-265 1.47e-07

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 52.18  E-value: 1.47e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  50 WSFVFSTLGVTAIAFVTGALGFWAPKFLFEarvVHGLQLpcfqeqchsQDSLIFGALTVATGIIGVMLGAEASRRYKkvn 129
Cdd:COG2271  180 LRFWLLALAYFLVYFALYGFLTWLPTYLVE---VRGLSL---------AQAGLLLSLPFLAGIVGSLLGGWLSDRLG--- 244

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391 130 pRAEPLICASSLFATAPCLYLALILASRTLLasYVFLALGELLLSCNWAVVADILLSVVVPRCRGTAEALqITVAHVLGD 209
Cdd:COG2271  245 -RRRKLVLAIGLLLAALALLLLALLPSPALA--IALLFLAGFGLGGAFGLLWALAAELFPKKARGTASGL-VNTFGFLGG 320

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 568976391 210 AGSPYLTGLISSvlqaerpdsylqhFLSLQHSFLCCAFAIVLGGGFFLLTALHLEK 265
Cdd:COG2271  321 ALGPLLVGYLLD-------------ATGYQAAFLLLAALALLAALLALLLLRETRK 363
MFS_ExuT_GudP_like cd17319
Hexuronate transporter, Glucarate transporter, and similar transporters of the Major ...
 50-258 9.73e-06

Hexuronate transporter, Glucarate transporter, and similar transporters of the Major Facilitator Superfamily; This family is composed of predominantly bacterial transporters for hexuronate (ExuT), glucarate (GudP), galactarate (GarP), and galactonate (DgoT). They mediate the uptake of these compounds into the cell. They belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340877 [Multi-domain]  Cd Length: 358  Bit Score: 46.41  E-value: 9.73e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  50 WSFVFSTLGVTAIAFVTGALGFWAPKFLFEARVVHGLQLpcfqeqchsqdslifGALTVATGIIGVMLGAEASRRYKKVN 129
Cdd:cd17319  179 RQVWGLALGYFGINYALYGLLTWLPTYLKQARGFSLKQV---------------GLLAAIPFLAGAIGMILGGRLSDRLL 243

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391 130 PRAEPLICASSLFATAPCLYLALILASrtLLASYVFLALGELLLSCNWAVVADILLSVVVPRCRGTAEALqITVAHVLGD 209
Cdd:cd17319  244 RGARRLVIIIGLLLAAVGLLLAYLTTS--PVVAVILLSLAGFGIGAASPVFWSLPADLLPGEAAATAGGL-INSGGNLGG 320

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 568976391 210 AGSPYLTGLIssvlqaerpdsyLQHFLSLQHSFLCCAFAIVLGGGFFLL 258
Cdd:cd17319  321 FVAPLLIGYL------------VDATGSYTAAFLFLAAVALLAALLVLL 357
MFS_SLCO4_OATP4 cd17403
Solute carrier organic anion transporter 4 family of the Major Facilitator Superfamily of ...
 50-182 2.02e-03

Solute carrier organic anion transporter 4 family of the Major Facilitator Superfamily of transporters; The Solute carrier organic anion transporter 4 (SLCO4) or Organic anion transporting polypeptide 4 (OATP4) family contains two families: OATP4A and OATP4C, each containing one mammalian member, OATP4A1 and OATP4C1, respectively. OATP4A1 (encoded by SLCO4A1), is ubiquitously expressed and mediates the Na(+)-independent transport of the thyroid hormones T3 (triiodo-L-thyronine), T4 (thyroxine) and rT3, and other organic anions such as estrone sulfate and taurocholate. OATP4C1 (encoded by SLCO4C1) is capable of transporting pharmacological substances such as digoxin, ouabain, thyroxine, methotrexate, cAMP, and uremic toxins, which accumulate in patients with chronic kidney diseases (CKDs). The SLCO4/OATP4 family belongs to the Solute carrier organic anion transporter [SLCO, also called organic anion transporting polypeptides (OATPs) or Solute carrier family 21] family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340961 [Multi-domain]  Cd Length: 420  Bit Score: 39.54  E-value: 2.02e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391  50 WSFVFSTLGVTAIAFVTGALGFWAPKFLFEArvvhgLQLPCfqeqchSQDSLIFGALTVATGIIGVMLGAEASRRYkKVN 129
Cdd:cd17403  200 PTFMFLNLAGASEGMLLAGFATFLPKLIESQ-----FSLSA------SLAALLVGVVVVPGGILGTFLGGLIVKKL-KLK 267

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 568976391 130 PRAEPLICassLFATAPCLYLALILASR---TLLASyVFLALGELLLSCNWAVVAD 182
Cdd:cd17403  268 CKGILKFC---IVCSIVAFLFSFILLIYcpnAPFAG-VTVPYGNSPLEGTSNLTST 319
OATP pfam03137
Organic Anion Transporter Polypeptide (OATP) family; This family consists of several ...
 43-176 9.71e-03

Organic Anion Transporter Polypeptide (OATP) family; This family consists of several eukaryotic Organic-Anion-Transporting Polypeptides (OATPs). Several have been identified mostly in human and rat. Different OATPs vary in tissue distribution and substrate specificity. Since the numbering of different OATPs in particular species was based originally on the order of discovery, similarly numbered OATPs in humans and rats did not necessarily correspond in function, tissue distribution and substrate specificity (in spite of the name, some OATPs also transport organic cations and neutral molecules). Thus, Tamai et al. initiated the current scheme of using digits for rat OATPs and letters for human ones. Prostaglandin transporter (PGT) proteins are also considered to be OATP family members. In addition, the methotrexate transporter OATK is closely related to OATPs. This family also includes several predicted proteins from Caenorhabditis elegans and Drosophila melanogaster. This similarity was not previously noted. Note: Members of this family are described (in the Swiss-Prot database) as belonging to the SLC21 family of transporters.


Pssm-ID: 460821 [Multi-domain]  Cd Length: 526  Bit Score: 37.57  E-value: 9.71e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568976391   43 VRYLGRNWSFVFSTLGvtAIAFVTGALGFWA--PKFLfearvvhglqlpcfQEQCH---SQDSLIFGALTVATGIIGVML 117
Cdd:pfam03137 239 LKRLLKNPVYMLLTLA--SVFEFLLIAGYATflPKYI--------------ETQFGlsaSTANLLTGVVAIPGAALGILL 302

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 568976391  118 GAEASRRYkKVNPRAEP-LICASSLFATAPCLYLALI------LASRTLLASYVFLALGELLLSCN 176
Cdd:pfam03137 303 GGLIIKKF-KLSAKGLAkFNVITSLVSLLLSLLLFFLgcpnspVAGITVPYGGTQQLSGNLTSSCN 367
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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