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Conserved domains on  [gi|755526559|ref|XP_011246638|]
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DDB1- and CUL4-associated factor 15 isoform X2 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DCAF15-CTD cd20913
C-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the C-terminal ...
 223-473 6.57e-72

C-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the C-terminal domain of DCAF15 (DDB1- and CUL4-associated factor 15), the cullin RING ligase substrate receptor/adaptor that forms a complex with CUL4A or CUL4B, as part of the Rbx-Cul4-DDA1-DDB1-DCAF15 E3 ubiquitin ligase that is responsible for the proteasome degradation of certain proteins. Aryl sulfonamide anticancer agents such as indisulam, tasisulam, E7820, and chloroquinoxaline have been shown to recruit the essential mRNA-splicing factor RBM39 to DCAF15. These agents appear to promote binding of DCAF15 to the RNA-recognition motif (RRM) of RBM39, which suggests that derivatives of the aryl-sulfonamides may be used to target other RRM-containing proteins. Cell proliferation is inhibited by these aryl sulfonamides by causing degradation of RBM39, which leads to aberrant processing of pre-mRNA in hundreds of genes, primarily reflected by intron retention and exon skipping, thus collectively referred to as splicing inhibitor sulfonamides, or SPLAMs.


:

Pssm-ID: 411023  Cd Length: 224  Bit Score: 226.78  E-value: 6.57e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 223 INYTKLHYVLQSGEG--TEPEDEFEDDK--ISLPFVVTDLRGRNLRPMRERTD--MQGQYLTVEQLTLDFEYVINEVIRH 296
Cdd:cd20913    1 VKKVVRRYSEVDDEIviTDIEDDSLLSGqhIELPLEVHGLGYQQLQMVSNAKAdkLTEPQVIVTQRSLDIEQFINEVAQK 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 297 D-ATWGHQFCSFSDYDIVILEVCPETNQVLINIGLLLLAFPAPTEE---GQLRPKTYHTSLKVAWDLNTGIFETVSVGDL 372
Cdd:cd20913   81 LcAEWGKKFWSCSDYDIEIVDVCPLSGDVICLLVILIQALESPEESkldCSNRRKLYETSCLFTWNISTGQYRTLDVGDL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 373 TEVKG-QTSGSVWSSYRKSCVDMVMKWLVPESSGRYVNRMTNEALHKGRkcpswaghlilpwipgmldvrsvlgsewhpa 451
Cdd:cd20913  161 TEVPPeQTSSQEWNPARKEASRLRKKWLVPQSSKRSVRVLTNESVFKGR------------------------------- 209

                        250       260
                 ....*....|....*....|..
gi 755526559 452 mageagcSLKVLADSERYTWIV 473
Cdd:cd20913  210 -------SLDNLIDPFHYSAIV 224
DCAF15-NTD super family cl20823
N-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the N-terminal ...
 9-95 7.31e-43

N-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the N-terminal domain of DCAF15 (DDB1- and CUL4-associated factor 15), the cullin RING ligase substrate receptor/adaptor that forms a complex with CUL4A or CUL4B, as part of the Rbx-Cul4-DDA1-DDB1-DCAF15 E3 ubiquitin ligase that is responsible for the proteasome degradation of certain proteins. Aryl sulfonamide anticancer agents such as indisulam, tasisulam, E7820, and chloroquinoxaline have been shown to recruit the essential mRNA-splicing factor RBM39 to DCAF15. These agents appear to promote binding of DCAF15 to the RNA-recognition motif (RRM) of RBM39, which suggests that derivatives of the aryl-sulfonamides may be used to target other RRM-containing proteins. Cell proliferation is inhibited by these aryl sulfonamides by causing degradation of RBM39, which leads to aberrant processing of pre-mRNA in hundreds of genes, primarily reflected by intron retention and exon skipping, thus collectively referred to as splicing inhibitor sulfonamides, or SPLAMs.


The actual alignment was detected with superfamily member pfam14939:

Pssm-ID: 473535  Cd Length: 206  Bit Score: 150.56  E-value: 7.31e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559    9 SDENHRDIYISTVAVPPRGRCAACQDASRAHPGDPSAQCLRHGFMLHTKYQVVYPFPTFQPAFQLKKDQVVLLNTSYSLV 88
Cdd:pfam14939 120 SDEEHRDIYITIVTVPSLSPCKDCKRVADSHPEEDNALCLRHGLTIHTKYEVVYPYPTFQPSVSLKCDNVVLLNTGNFLH 199


                  ....*..
gi 755526559   89 ACAVSVH 95
Cdd:pfam14939 200 ALAVDVD 206
 
Name Accession Description Interval E-value
DCAF15-CTD cd20913
C-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the C-terminal ...
 223-473 6.57e-72

C-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the C-terminal domain of DCAF15 (DDB1- and CUL4-associated factor 15), the cullin RING ligase substrate receptor/adaptor that forms a complex with CUL4A or CUL4B, as part of the Rbx-Cul4-DDA1-DDB1-DCAF15 E3 ubiquitin ligase that is responsible for the proteasome degradation of certain proteins. Aryl sulfonamide anticancer agents such as indisulam, tasisulam, E7820, and chloroquinoxaline have been shown to recruit the essential mRNA-splicing factor RBM39 to DCAF15. These agents appear to promote binding of DCAF15 to the RNA-recognition motif (RRM) of RBM39, which suggests that derivatives of the aryl-sulfonamides may be used to target other RRM-containing proteins. Cell proliferation is inhibited by these aryl sulfonamides by causing degradation of RBM39, which leads to aberrant processing of pre-mRNA in hundreds of genes, primarily reflected by intron retention and exon skipping, thus collectively referred to as splicing inhibitor sulfonamides, or SPLAMs.


Pssm-ID: 411023  Cd Length: 224  Bit Score: 226.78  E-value: 6.57e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 223 INYTKLHYVLQSGEG--TEPEDEFEDDK--ISLPFVVTDLRGRNLRPMRERTD--MQGQYLTVEQLTLDFEYVINEVIRH 296
Cdd:cd20913    1 VKKVVRRYSEVDDEIviTDIEDDSLLSGqhIELPLEVHGLGYQQLQMVSNAKAdkLTEPQVIVTQRSLDIEQFINEVAQK 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 297 D-ATWGHQFCSFSDYDIVILEVCPETNQVLINIGLLLLAFPAPTEE---GQLRPKTYHTSLKVAWDLNTGIFETVSVGDL 372
Cdd:cd20913   81 LcAEWGKKFWSCSDYDIEIVDVCPLSGDVICLLVILIQALESPEESkldCSNRRKLYETSCLFTWNISTGQYRTLDVGDL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 373 TEVKG-QTSGSVWSSYRKSCVDMVMKWLVPESSGRYVNRMTNEALHKGRkcpswaghlilpwipgmldvrsvlgsewhpa 451
Cdd:cd20913  161 TEVPPeQTSSQEWNPARKEASRLRKKWLVPQSSKRSVRVLTNESVFKGR------------------------------- 209

                        250       260
                 ....*....|....*....|..
gi 755526559 452 mageagcSLKVLADSERYTWIV 473
Cdd:cd20913  210 -------SLDNLIDPFHYSAIV 224
DCAF15_WD40 pfam14939
DDB1-and CUL4-substrate receptor 15, WD repeat; DCAFs, Ddb1- and Cul4-associated factors, are ...
 9-95 7.31e-43

DDB1-and CUL4-substrate receptor 15, WD repeat; DCAFs, Ddb1- and Cul4-associated factors, are substrate receptors for the Cul4-Ddb1 Ubiquitin Ligase. There are 18 different factors, the majority of which are WD40-repeat-proteins.


Pssm-ID: 464387  Cd Length: 206  Bit Score: 150.56  E-value: 7.31e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559    9 SDENHRDIYISTVAVPPRGRCAACQDASRAHPGDPSAQCLRHGFMLHTKYQVVYPFPTFQPAFQLKKDQVVLLNTSYSLV 88
Cdd:pfam14939 120 SDEEHRDIYITIVTVPSLSPCKDCKRVADSHPEEDNALCLRHGLTIHTKYEVVYPYPTFQPSVSLKCDNVVLLNTGNFLH 199


                  ....*..
gi 755526559   89 ACAVSVH 95
Cdd:pfam14939 200 ALAVDVD 206
DCAF15-NTD cd20917
N-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the N-terminal ...
 9-95 3.13e-29

N-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the N-terminal domain of DCAF15 (DDB1- and CUL4-associated factor 15), the cullin RING ligase substrate receptor/adaptor that forms a complex with CUL4A or CUL4B, as part of the Rbx-Cul4-DDA1-DDB1-DCAF15 E3 ubiquitin ligase that is responsible for the proteasome degradation of certain proteins. Aryl sulfonamide anticancer agents such as indisulam, tasisulam, E7820, and chloroquinoxaline have been shown to recruit the essential mRNA-splicing factor RBM39 to DCAF15. These agents appear to promote binding of DCAF15 to the RNA-recognition motif (RRM) of RBM39, which suggests that derivatives of the aryl-sulfonamides may be used to target other RRM-containing proteins. Cell proliferation is inhibited by these aryl sulfonamides by causing degradation of RBM39, which leads to aberrant processing of pre-mRNA in hundreds of genes, primarily reflected by intron retention and exon skipping, thus collectively referred to as splicing inhibitor sulfonamides, or SPLAMs.


Pssm-ID: 411024  Cd Length: 225  Bit Score: 114.34  E-value: 3.13e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559   9 SDENHRDIYISTVAVPPRGRCAACQDASRAH-PGDPSAQCLRHGFMLHTKYQVVYPFPTFQPAFQLKKDQVVLLNTSYSL 87
Cdd:cd20917  138 SPEESRDCYITITAVPSLSNCPDCKQLALSNwDSCSRFRCLKHGFTVHTKYELVPPFPPFDPSVSLKLPGVVVLNTGNFL 217


                 ....*...
gi 755526559  88 VACAVSVH 95
Cdd:cd20917  218 HVLSVSLE 225
 
Name Accession Description Interval E-value
DCAF15-CTD cd20913
C-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the C-terminal ...
 223-473 6.57e-72

C-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the C-terminal domain of DCAF15 (DDB1- and CUL4-associated factor 15), the cullin RING ligase substrate receptor/adaptor that forms a complex with CUL4A or CUL4B, as part of the Rbx-Cul4-DDA1-DDB1-DCAF15 E3 ubiquitin ligase that is responsible for the proteasome degradation of certain proteins. Aryl sulfonamide anticancer agents such as indisulam, tasisulam, E7820, and chloroquinoxaline have been shown to recruit the essential mRNA-splicing factor RBM39 to DCAF15. These agents appear to promote binding of DCAF15 to the RNA-recognition motif (RRM) of RBM39, which suggests that derivatives of the aryl-sulfonamides may be used to target other RRM-containing proteins. Cell proliferation is inhibited by these aryl sulfonamides by causing degradation of RBM39, which leads to aberrant processing of pre-mRNA in hundreds of genes, primarily reflected by intron retention and exon skipping, thus collectively referred to as splicing inhibitor sulfonamides, or SPLAMs.


Pssm-ID: 411023  Cd Length: 224  Bit Score: 226.78  E-value: 6.57e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 223 INYTKLHYVLQSGEG--TEPEDEFEDDK--ISLPFVVTDLRGRNLRPMRERTD--MQGQYLTVEQLTLDFEYVINEVIRH 296
Cdd:cd20913    1 VKKVVRRYSEVDDEIviTDIEDDSLLSGqhIELPLEVHGLGYQQLQMVSNAKAdkLTEPQVIVTQRSLDIEQFINEVAQK 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 297 D-ATWGHQFCSFSDYDIVILEVCPETNQVLINIGLLLLAFPAPTEE---GQLRPKTYHTSLKVAWDLNTGIFETVSVGDL 372
Cdd:cd20913   81 LcAEWGKKFWSCSDYDIEIVDVCPLSGDVICLLVILIQALESPEESkldCSNRRKLYETSCLFTWNISTGQYRTLDVGDL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559 373 TEVKG-QTSGSVWSSYRKSCVDMVMKWLVPESSGRYVNRMTNEALHKGRkcpswaghlilpwipgmldvrsvlgsewhpa 451
Cdd:cd20913  161 TEVPPeQTSSQEWNPARKEASRLRKKWLVPQSSKRSVRVLTNESVFKGR------------------------------- 209

                        250       260
                 ....*....|....*....|..
gi 755526559 452 mageagcSLKVLADSERYTWIV 473
Cdd:cd20913  210 -------SLDNLIDPFHYSAIV 224
DCAF15_WD40 pfam14939
DDB1-and CUL4-substrate receptor 15, WD repeat; DCAFs, Ddb1- and Cul4-associated factors, are ...
 9-95 7.31e-43

DDB1-and CUL4-substrate receptor 15, WD repeat; DCAFs, Ddb1- and Cul4-associated factors, are substrate receptors for the Cul4-Ddb1 Ubiquitin Ligase. There are 18 different factors, the majority of which are WD40-repeat-proteins.


Pssm-ID: 464387  Cd Length: 206  Bit Score: 150.56  E-value: 7.31e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559    9 SDENHRDIYISTVAVPPRGRCAACQDASRAHPGDPSAQCLRHGFMLHTKYQVVYPFPTFQPAFQLKKDQVVLLNTSYSLV 88
Cdd:pfam14939 120 SDEEHRDIYITIVTVPSLSPCKDCKRVADSHPEEDNALCLRHGLTIHTKYEVVYPYPTFQPSVSLKCDNVVLLNTGNFLH 199


                  ....*..
gi 755526559   89 ACAVSVH 95
Cdd:pfam14939 200 ALAVDVD 206
DCAF15-NTD cd20917
N-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the N-terminal ...
 9-95 3.13e-29

N-terminal domain of DDB1- and CUL4-associated factor 15; This model represents the N-terminal domain of DCAF15 (DDB1- and CUL4-associated factor 15), the cullin RING ligase substrate receptor/adaptor that forms a complex with CUL4A or CUL4B, as part of the Rbx-Cul4-DDA1-DDB1-DCAF15 E3 ubiquitin ligase that is responsible for the proteasome degradation of certain proteins. Aryl sulfonamide anticancer agents such as indisulam, tasisulam, E7820, and chloroquinoxaline have been shown to recruit the essential mRNA-splicing factor RBM39 to DCAF15. These agents appear to promote binding of DCAF15 to the RNA-recognition motif (RRM) of RBM39, which suggests that derivatives of the aryl-sulfonamides may be used to target other RRM-containing proteins. Cell proliferation is inhibited by these aryl sulfonamides by causing degradation of RBM39, which leads to aberrant processing of pre-mRNA in hundreds of genes, primarily reflected by intron retention and exon skipping, thus collectively referred to as splicing inhibitor sulfonamides, or SPLAMs.


Pssm-ID: 411024  Cd Length: 225  Bit Score: 114.34  E-value: 3.13e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755526559   9 SDENHRDIYISTVAVPPRGRCAACQDASRAH-PGDPSAQCLRHGFMLHTKYQVVYPFPTFQPAFQLKKDQVVLLNTSYSL 87
Cdd:cd20917  138 SPEESRDCYITITAVPSLSNCPDCKQLALSNwDSCSRFRCLKHGFTVHTKYELVPPFPPFDPSVSLKLPGVVVLNTGNFL 217


                 ....*...
gi 755526559  88 VACAVSVH 95
Cdd:cd20917  218 HVLSVSLE 225
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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