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Conserved domains on  [gi|767994401|ref|XP_011522958|]
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endonuclease V isoform X2 [Homo sapiens]

Protein Classification

endonuclease V( domain architecture ID 10158987)

endonuclease V selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Endonuclease_V cd06559
Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine ...
17-258 1.23e-85

Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine bases; Endonuclease_V (EndoV) is an enzyme that can initiate repair of all possible deaminated DNA bases. EndoV cleaves the DNA strand containing lesions at the second phosphodiester bond 3' to the lesion using Mg2+ as a cofactor. EndoV homologs are conserved throughout all domains of life from bacteria to humans. EndoV is encoded by the nfi gene and nfi null mutant mice have a phenotype prone to cancer. The ability of endonuclease V to recognize mismatches and abnormal replicative DNA structures suggests that the enzyme plays an important role in DNA metabolism. The details of downstream processing for the EndoV pathway remain unknown.


:

Pssm-ID: 143472  Cd Length: 208  Bit Score: 258.58  E-value: 1.23e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  17 WKREQARLKAHVVDRDTEawqrdpaFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFL 96
Cdd:cd06559    4 ARKLQEELAKKVVLEDLG-------PGEVRLVAGVDVSYKKDGD-LAVAAAVVLDYPDLEVVETAVAVGEVTFPYIPGLL 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  97 AFREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHkekirll 176
Cdd:cd06559   76 AFREGPPLLEALEKLKTK-----PDLLLVDGHGIAHPRRFGLASHLGVLLDLPTIGVAKSLLVGDGEEPGEER------- 143
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401 177 qtrgDSFPLLGDSGTVLGMptgqpspslsqALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCC-RFRIPEPVRQVHLLlc 255
Cdd:cd06559  144 ----GSFTPLYDDGEVVGA-----------ALRTRDG-VKPVYVSPGHRIDLETAVELVLKCCkGYRLPEPTRLADLL-- 205

                 ...
gi 767994401 256 SRR 258
Cdd:cd06559  206 SRE 208
 
Name Accession Description Interval E-value
Endonuclease_V cd06559
Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine ...
17-258 1.23e-85

Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine bases; Endonuclease_V (EndoV) is an enzyme that can initiate repair of all possible deaminated DNA bases. EndoV cleaves the DNA strand containing lesions at the second phosphodiester bond 3' to the lesion using Mg2+ as a cofactor. EndoV homologs are conserved throughout all domains of life from bacteria to humans. EndoV is encoded by the nfi gene and nfi null mutant mice have a phenotype prone to cancer. The ability of endonuclease V to recognize mismatches and abnormal replicative DNA structures suggests that the enzyme plays an important role in DNA metabolism. The details of downstream processing for the EndoV pathway remain unknown.


Pssm-ID: 143472  Cd Length: 208  Bit Score: 258.58  E-value: 1.23e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  17 WKREQARLKAHVVDRDTEawqrdpaFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFL 96
Cdd:cd06559    4 ARKLQEELAKKVVLEDLG-------PGEVRLVAGVDVSYKKDGD-LAVAAAVVLDYPDLEVVETAVAVGEVTFPYIPGLL 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  97 AFREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHkekirll 176
Cdd:cd06559   76 AFREGPPLLEALEKLKTK-----PDLLLVDGHGIAHPRRFGLASHLGVLLDLPTIGVAKSLLVGDGEEPGEER------- 143
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401 177 qtrgDSFPLLGDSGTVLGMptgqpspslsqALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCC-RFRIPEPVRQVHLLlc 255
Cdd:cd06559  144 ----GSFTPLYDDGEVVGA-----------ALRTRDG-VKPVYVSPGHRIDLETAVELVLKCCkGYRLPEPTRLADLL-- 205

                 ...
gi 767994401 256 SRR 258
Cdd:cd06559  206 SRE 208
Endonuclease_5 pfam04493
Endonuclease V; Endonuclease V is specific for single-stranded DNA or for duplex DNA that ...
46-252 9.48e-76

Endonuclease V; Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents.


Pssm-ID: 427979  Cd Length: 198  Bit Score: 232.73  E-value: 9.48e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401   46 QRVGGVDVSFVKGD-SVRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLAFREVPFLLELVQQLREKepglmPQVLL 124
Cdd:pfam04493  18 RLVAGVDVSFVKDDdGGTAVAAIVVLDYPTLEVVEEAVAVGPVTFPYIPGLLSFRELPALLEALEKLKQK-----PDLLL 92
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  125 VDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLlqvdglennaLHKEKIRLLQTRGDSFPLLgDSGTVLGMptgqpspsl 204
Cdd:pfam04493  93 VDGQGIAHPRRLGIASHLGVLLDLPTIGVAKSR----------LVGKFEEPGEERGSYSPLV-DKGEVVGA--------- 152
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 767994401  205 sqALRSHDRStRPLYISVGHRMSLEAAVRLTC-CCCRFRIPEPVRQVHL 252
Cdd:pfam04493 153 --VLRTRKNV-KPIYVSPGHRISLDTALELVLrCLRGYRLPEPTRLADL 198
Nfi COG1515
Deoxyinosine 3'-endonuclease (endonuclease V) [Replication, recombination and repair];
18-253 2.79e-66

Deoxyinosine 3'-endonuclease (endonuclease V) [Replication, recombination and repair];


Pssm-ID: 441124  Cd Length: 221  Bit Score: 209.22  E-value: 2.79e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  18 KREQARLKAHVVdrdteawqRDPAFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLA 97
Cdd:COG1515   14 RALQRELAARVV--------LEDDLGEPRLVAGVDVSYEDGGD-RAVAAIVVLDYPTLEVVERAVARGPVTFPYIPGLLS 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  98 FREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLL--QVDGLENNalhkekirl 175
Cdd:COG1515   85 FRELPALLAALEKLKVE-----PDLLLVDGQGIAHPRRLGIASHLGVLLDVPTIGVAKSRLcgEFEEPGEE--------- 150
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 767994401 176 lqtRGDSFPLLgDSGTVLGMptgqpspslsqALRSHDRsTRPLYISVGHRMSLEAAVRLT-CCCCRFRIPEPVRQVHLL 253
Cdd:COG1515  151 ---RGSWSPLV-DDGEVIGA-----------VLRTRDG-VKPLYVSPGHRISLETALELVlACTPGYRLPEPTRLADRL 213
PRK11617 PRK11617
deoxyribonuclease V;
18-258 3.91e-48

deoxyribonuclease V;


Pssm-ID: 236937  Cd Length: 224  Bit Score: 162.80  E-value: 3.91e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  18 KREQARLKAHVVDRDteawqrDPAFSGLQRVGGVDVSFVKGDSVRAcASLVVLSFPELEVVYEESRMVSLTAPYVSGFLA 97
Cdd:PRK11617   8 RAQQIELASSVIRED------RLDKDPPRLIAGADVGFEQGGEVTR-AAIVVLKYPSLELVEYQVARIATTMPYIPGFLS 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  98 FREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDglennalHKEkirLLQ 177
Cdd:PRK11617  81 FREYPALLAAWEQLSQK-----PDLVFVDGHGIAHPRRLGVASHFGLLVDVPTIGVAKKRLCGK-------FEP---LSE 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401 178 TRGDSFPLLgDSGTVLGMptgqpspslsqALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCCR-FRIPEPVRQVHlLLCS 256
Cdd:PRK11617 146 EPGSLQPLM-DKGEQLGW-----------VWRSKAR-CNPLFISTGHRVSLDSALAWVQRCMKgYRLPEPTRWAD-ALAS 211

                 ..
gi 767994401 257 RR 258
Cdd:PRK11617 212 RR 213
 
Name Accession Description Interval E-value
Endonuclease_V cd06559
Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine ...
17-258 1.23e-85

Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine bases; Endonuclease_V (EndoV) is an enzyme that can initiate repair of all possible deaminated DNA bases. EndoV cleaves the DNA strand containing lesions at the second phosphodiester bond 3' to the lesion using Mg2+ as a cofactor. EndoV homologs are conserved throughout all domains of life from bacteria to humans. EndoV is encoded by the nfi gene and nfi null mutant mice have a phenotype prone to cancer. The ability of endonuclease V to recognize mismatches and abnormal replicative DNA structures suggests that the enzyme plays an important role in DNA metabolism. The details of downstream processing for the EndoV pathway remain unknown.


Pssm-ID: 143472  Cd Length: 208  Bit Score: 258.58  E-value: 1.23e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  17 WKREQARLKAHVVDRDTEawqrdpaFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFL 96
Cdd:cd06559    4 ARKLQEELAKKVVLEDLG-------PGEVRLVAGVDVSYKKDGD-LAVAAAVVLDYPDLEVVETAVAVGEVTFPYIPGLL 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  97 AFREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHkekirll 176
Cdd:cd06559   76 AFREGPPLLEALEKLKTK-----PDLLLVDGHGIAHPRRFGLASHLGVLLDLPTIGVAKSLLVGDGEEPGEER------- 143
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401 177 qtrgDSFPLLGDSGTVLGMptgqpspslsqALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCC-RFRIPEPVRQVHLLlc 255
Cdd:cd06559  144 ----GSFTPLYDDGEVVGA-----------ALRTRDG-VKPVYVSPGHRIDLETAVELVLKCCkGYRLPEPTRLADLL-- 205

                 ...
gi 767994401 256 SRR 258
Cdd:cd06559  206 SRE 208
Endonuclease_5 pfam04493
Endonuclease V; Endonuclease V is specific for single-stranded DNA or for duplex DNA that ...
46-252 9.48e-76

Endonuclease V; Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents.


Pssm-ID: 427979  Cd Length: 198  Bit Score: 232.73  E-value: 9.48e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401   46 QRVGGVDVSFVKGD-SVRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLAFREVPFLLELVQQLREKepglmPQVLL 124
Cdd:pfam04493  18 RLVAGVDVSFVKDDdGGTAVAAIVVLDYPTLEVVEEAVAVGPVTFPYIPGLLSFRELPALLEALEKLKQK-----PDLLL 92
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  125 VDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLlqvdglennaLHKEKIRLLQTRGDSFPLLgDSGTVLGMptgqpspsl 204
Cdd:pfam04493  93 VDGQGIAHPRRLGIASHLGVLLDLPTIGVAKSR----------LVGKFEEPGEERGSYSPLV-DKGEVVGA--------- 152
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 767994401  205 sqALRSHDRStRPLYISVGHRMSLEAAVRLTC-CCCRFRIPEPVRQVHL 252
Cdd:pfam04493 153 --VLRTRKNV-KPIYVSPGHRISLDTALELVLrCLRGYRLPEPTRLADL 198
Nfi COG1515
Deoxyinosine 3'-endonuclease (endonuclease V) [Replication, recombination and repair];
18-253 2.79e-66

Deoxyinosine 3'-endonuclease (endonuclease V) [Replication, recombination and repair];


Pssm-ID: 441124  Cd Length: 221  Bit Score: 209.22  E-value: 2.79e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  18 KREQARLKAHVVdrdteawqRDPAFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLA 97
Cdd:COG1515   14 RALQRELAARVV--------LEDDLGEPRLVAGVDVSYEDGGD-RAVAAIVVLDYPTLEVVERAVARGPVTFPYIPGLLS 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  98 FREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLL--QVDGLENNalhkekirl 175
Cdd:COG1515   85 FRELPALLAALEKLKVE-----PDLLLVDGQGIAHPRRLGIASHLGVLLDVPTIGVAKSRLcgEFEEPGEE--------- 150
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 767994401 176 lqtRGDSFPLLgDSGTVLGMptgqpspslsqALRSHDRsTRPLYISVGHRMSLEAAVRLT-CCCCRFRIPEPVRQVHLL 253
Cdd:COG1515  151 ---RGSWSPLV-DDGEVIGA-----------VLRTRDG-VKPLYVSPGHRISLETALELVlACTPGYRLPEPTRLADRL 213
PRK11617 PRK11617
deoxyribonuclease V;
18-258 3.91e-48

deoxyribonuclease V;


Pssm-ID: 236937  Cd Length: 224  Bit Score: 162.80  E-value: 3.91e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  18 KREQARLKAHVVDRDteawqrDPAFSGLQRVGGVDVSFVKGDSVRAcASLVVLSFPELEVVYEESRMVSLTAPYVSGFLA 97
Cdd:PRK11617   8 RAQQIELASSVIRED------RLDKDPPRLIAGADVGFEQGGEVTR-AAIVVLKYPSLELVEYQVARIATTMPYIPGFLS 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401  98 FREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDglennalHKEkirLLQ 177
Cdd:PRK11617  81 FREYPALLAAWEQLSQK-----PDLVFVDGHGIAHPRRLGVASHFGLLVDVPTIGVAKKRLCGK-------FEP---LSE 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767994401 178 TRGDSFPLLgDSGTVLGMptgqpspslsqALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCCR-FRIPEPVRQVHlLLCS 256
Cdd:PRK11617 146 EPGSLQPLM-DKGEQLGW-----------VWRSKAR-CNPLFISTGHRVSLDSALAWVQRCMKgYRLPEPTRWAD-ALAS 211

                 ..
gi 767994401 257 RR 258
Cdd:PRK11617 212 RR 213
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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