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Conserved domains on  [gi|767941903|ref|XP_011533745|]
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tRNA-specific adenosine deaminase 2 isoform X4 [Homo sapiens]

Protein Classification

tRNA-specific adenosine deaminase( domain architecture ID 1903363)

tRNA-specific adenosine deaminases generate inosine at the first position of their anticodon (position 34) of specific tRNAs

EC:  3.5.4.33
Gene Ontology:  GO:0052717|GO:0006400|GO:0008251|GO:0046872

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TadA super family cl43199
tRNA(Arg) A34 adenosine deaminase TadA [Translation, ribosomal structure and biogenesis]; tRNA ...
 72-107 1.85e-07

tRNA(Arg) A34 adenosine deaminase TadA [Translation, ribosomal structure and biogenesis]; tRNA(Arg) A34 adenosine deaminase TadA is part of the Pathway/BioSystem: Pyrimidine salvagetRNA modification


The actual alignment was detected with superfamily member COG0590:

Pssm-ID: 440355 [Multi-domain]  Cd Length: 148  Bit Score: 47.04  E-value: 1.85e-07
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 767941903  72 AKEALENTEVPVGCLMVYNNEVVGKGRNEVNQTKNV 107
Cdd:COG0590   15 ARKAVAEGEVPVGAVLVKDGEIIARGHNRVETLNDP 50
 
Name Accession Description Interval E-value
TadA COG0590
tRNA(Arg) A34 adenosine deaminase TadA [Translation, ribosomal structure and biogenesis]; tRNA ...
 72-107 1.85e-07

tRNA(Arg) A34 adenosine deaminase TadA [Translation, ribosomal structure and biogenesis]; tRNA(Arg) A34 adenosine deaminase TadA is part of the Pathway/BioSystem: Pyrimidine salvagetRNA modification


Pssm-ID: 440355 [Multi-domain]  Cd Length: 148  Bit Score: 47.04  E-value: 1.85e-07
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 767941903  72 AKEALENTEVPVGCLMVYNNEVVGKGRNEVNQTKNV 107
Cdd:COG0590   15 ARKAVAEGEVPVGAVLVKDGEIIARGHNRVETLNDP 50
nucleoside_deaminase cd01285
Nucleoside deaminases include adenosine, guanine and cytosine deaminases. These enzymes are Zn ...
 72-106 4.36e-06

Nucleoside deaminases include adenosine, guanine and cytosine deaminases. These enzymes are Zn dependent and catalyze the deamination of nucleosides. The zinc ion in the active site plays a central role in the proposed catalytic mechanism, activating a water molecule to form a hydroxide ion that performs a nucleophilic attack on the substrate. The functional enzyme is a homodimer. Cytosine deaminase catalyzes the deamination of cytosine to uracil and ammonia and is a member of the pyrimidine salvage pathway. Cytosine deaminase is found in bacteria and fungi but is not present in mammals; for this reason, the enzyme is currently of interest for antimicrobial drug design and gene therapy applications against tumors. Some members of this family are tRNA-specific adenosine deaminases that generate inosine at the first position of their anticodon (position 34) of specific tRNAs; this modification is thought to enlarge the codon recognition capacity during protein synthesis. Other members of the family are guanine deaminases which deaminate guanine to xanthine as part of the utilization of guanine as a nitrogen source.


Pssm-ID: 238612 [Multi-domain]  Cd Length: 109  Bit Score: 42.60  E-value: 4.36e-06
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 767941903  72 AKEALENTEVPVGCLMVYNN-EVVGKGRNEVNQTKN 106
Cdd:cd01285    8 ARKALAEGEVPFGAVIVDDDgKVIARGHNRVEQDGD 43
PRK10860 PRK10860
tRNA-specific adenosine deaminase; Provisional
 72-99 1.06e-03

tRNA-specific adenosine deaminase; Provisional


Pssm-ID: 182786 [Multi-domain]  Cd Length: 172  Bit Score: 37.09  E-value: 1.06e-03
                         10        20
                 ....*....|....*....|....*...
gi 767941903  72 AKEALENTEVPVGCLMVYNNEVVGKGRN 99
Cdd:PRK10860  24 AKRAWDEREVPVGAVLVHNNRVIGEGWN 51
 
Name Accession Description Interval E-value
TadA COG0590
tRNA(Arg) A34 adenosine deaminase TadA [Translation, ribosomal structure and biogenesis]; tRNA ...
 72-107 1.85e-07

tRNA(Arg) A34 adenosine deaminase TadA [Translation, ribosomal structure and biogenesis]; tRNA(Arg) A34 adenosine deaminase TadA is part of the Pathway/BioSystem: Pyrimidine salvagetRNA modification


Pssm-ID: 440355 [Multi-domain]  Cd Length: 148  Bit Score: 47.04  E-value: 1.85e-07
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 767941903  72 AKEALENTEVPVGCLMVYNNEVVGKGRNEVNQTKNV 107
Cdd:COG0590   15 ARKAVAEGEVPVGAVLVKDGEIIARGHNRVETLNDP 50
nucleoside_deaminase cd01285
Nucleoside deaminases include adenosine, guanine and cytosine deaminases. These enzymes are Zn ...
 72-106 4.36e-06

Nucleoside deaminases include adenosine, guanine and cytosine deaminases. These enzymes are Zn dependent and catalyze the deamination of nucleosides. The zinc ion in the active site plays a central role in the proposed catalytic mechanism, activating a water molecule to form a hydroxide ion that performs a nucleophilic attack on the substrate. The functional enzyme is a homodimer. Cytosine deaminase catalyzes the deamination of cytosine to uracil and ammonia and is a member of the pyrimidine salvage pathway. Cytosine deaminase is found in bacteria and fungi but is not present in mammals; for this reason, the enzyme is currently of interest for antimicrobial drug design and gene therapy applications against tumors. Some members of this family are tRNA-specific adenosine deaminases that generate inosine at the first position of their anticodon (position 34) of specific tRNAs; this modification is thought to enlarge the codon recognition capacity during protein synthesis. Other members of the family are guanine deaminases which deaminate guanine to xanthine as part of the utilization of guanine as a nitrogen source.


Pssm-ID: 238612 [Multi-domain]  Cd Length: 109  Bit Score: 42.60  E-value: 4.36e-06
                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 767941903  72 AKEALENTEVPVGCLMVYNN-EVVGKGRNEVNQTKN 106
Cdd:cd01285    8 ARKALAEGEVPFGAVIVDDDgKVIARGHNRVEQDGD 43
PRK10860 PRK10860
tRNA-specific adenosine deaminase; Provisional
 72-99 1.06e-03

tRNA-specific adenosine deaminase; Provisional


Pssm-ID: 182786 [Multi-domain]  Cd Length: 172  Bit Score: 37.09  E-value: 1.06e-03
                         10        20
                 ....*....|....*....|....*...
gi 767941903  72 AKEALENTEVPVGCLMVYNNEVVGKGRN 99
Cdd:PRK10860  24 AKRAWDEREVPVGAVLVHNNRVIGEGWN 51
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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