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Conserved domains on  [gi|1720373124|ref|XP_030102813|]
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DDB1- and CUL4-associated factor 4 isoform X3 [Mus musculus]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 313-470 7.61e-13

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 68.90  E-value: 7.61e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 313 VVTGHrqsyriNSDVLAQQFAVKTPLLFNGCRSGEIFAIDLRSpsqakgWKATQIF--HDSAVTSVQVFKEEQHLMASDM 390
Cdd:cd00200   130 TLRGH------TDWVNSVAFSPDGTFVASSSQDGTIKLWDLRT------GKCVATLtgHTGEVNSVAFSPDGEKLLSSSS 197

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 391 SGKIKLWDLRATKCVRQYEGH---VNEYAYLPLHMheeegILVAVGQDCYTRIWSLHDARLLRTIPSPcptsKANIPSVA 467
Cdd:cd00200   198 DGTIKLWDLSTGKCLGTLRGHengVNSVAFSPDGY-----LLASGSEDGTIRVWDLRTGECVQTLSGH----TNSVTSLA 268


                  ...
gi 1720373124 468 FSP 470
Cdd:cd00200   269 WSP 271
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 313-470 7.61e-13

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 68.90  E-value: 7.61e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 313 VVTGHrqsyriNSDVLAQQFAVKTPLLFNGCRSGEIFAIDLRSpsqakgWKATQIF--HDSAVTSVQVFKEEQHLMASDM 390
Cdd:cd00200   130 TLRGH------TDWVNSVAFSPDGTFVASSSQDGTIKLWDLRT------GKCVATLtgHTGEVNSVAFSPDGEKLLSSSS 197

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 391 SGKIKLWDLRATKCVRQYEGH---VNEYAYLPLHMheeegILVAVGQDCYTRIWSLHDARLLRTIPSPcptsKANIPSVA 467
Cdd:cd00200   198 DGTIKLWDLSTGKCLGTLRGHengVNSVAFSPDGY-----LLASGSEDGTIRVWDLRTGECVQTLSGH----TNSVTSLA 268


                  ...
gi 1720373124 468 FSP 470
Cdd:cd00200   269 WSP 271
WD40 COG2319
WD40 repeat [General function prediction only];
369-470 2.19e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 59.54  E-value: 2.19e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 369 HDSAVTSVQVFKEEQHLMASDMSGKIKLWDLRATKCVRQYEGH---VNEYAYLPlhmheeEG-ILVAVGQDCYTRIWSLH 444
Cdd:COG2319   161 HSGAVTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHtgaVRSVAFSP------DGkLLASGSADGTVRLWDLA 234

                          90       100
                  ....*....|....*....|....*.
gi 1720373124 445 DARLLRTIPSPcptsKANIPSVAFSP 470
Cdd:COG2319   235 TGKLLRTLTGH----SGSVRSVAFSP 256
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 313-470 7.61e-13

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 68.90  E-value: 7.61e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 313 VVTGHrqsyriNSDVLAQQFAVKTPLLFNGCRSGEIFAIDLRSpsqakgWKATQIF--HDSAVTSVQVFKEEQHLMASDM 390
Cdd:cd00200   130 TLRGH------TDWVNSVAFSPDGTFVASSSQDGTIKLWDLRT------GKCVATLtgHTGEVNSVAFSPDGEKLLSSSS 197

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 391 SGKIKLWDLRATKCVRQYEGH---VNEYAYLPLHMheeegILVAVGQDCYTRIWSLHDARLLRTIPSPcptsKANIPSVA 467
Cdd:cd00200   198 DGTIKLWDLSTGKCLGTLRGHengVNSVAFSPDGY-----LLASGSEDGTIRVWDLRTGECVQTLSGH----TNSVTSLA 268


                  ...
gi 1720373124 468 FSP 470
Cdd:cd00200   269 WSP 271
WD40 COG2319
WD40 repeat [General function prediction only];
369-470 2.19e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 59.54  E-value: 2.19e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 369 HDSAVTSVQVFKEEQHLMASDMSGKIKLWDLRATKCVRQYEGH---VNEYAYLPlhmheeEG-ILVAVGQDCYTRIWSLH 444
Cdd:COG2319   161 HSGAVTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHtgaVRSVAFSP------DGkLLASGSADGTVRLWDLA 234

                          90       100
                  ....*....|....*....|....*.
gi 1720373124 445 DARLLRTIPSPcptsKANIPSVAFSP 470
Cdd:COG2319   235 TGKLLRTLTGH----SGSVRSVAFSP 256
WD40 COG2319
WD40 repeat [General function prediction only];
344-470 3.32e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 58.77  E-value: 3.32e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 344 RSGEIFAIDLRSPSQAKGWKATQIFHDSAVTSVQVFKEEQHLMASDMSGKIKLWDLRATKCVRQYEGH---VNEYAYLPl 420
Cdd:COG2319    52 AAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPDGRLLASASADGTVRLWDLATGLLLRTLTGHtgaVRSVAFSP- 130

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1720373124 421 hmheeEG-ILVAVGQDCYTRIWSLHDARLLRTIPSPcptsKANIPSVAFSP 470
Cdd:COG2319   131 -----DGkTLASGSADGTVRLWDLATGKLLRTLTGH----SGAVTSVAFSP 172
WD40 COG2319
WD40 repeat [General function prediction only];
369-470 1.43e-08

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 56.84  E-value: 1.43e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 369 HDSAVTSVQVFKEEQHLMASDMSGKIKLWDLRATKCVRQYEGH---VNEYAYLPlhmheeEG-ILVAVGQDCYTRIWSLH 444
Cdd:COG2319   245 HSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGHsggVNSVAFSP------DGkLLASGSDDGTVRLWDLA 318

                          90       100
                  ....*....|....*....|....*.
gi 1720373124 445 DARLLRTIPSPcptsKANIPSVAFSP 470
Cdd:COG2319   319 TGKLLRTLTGH----TGAVRSVAFSP 340
WD40 COG2319
WD40 repeat [General function prediction only];
369-470 1.53e-08

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 56.84  E-value: 1.53e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 369 HDSAVTSVQVFKEEQHLMASDMSGKIKLWDLRATKCVRQYEGH---VNEYAYLPlhmheEEGILVAVGQDCYTRIWSLHD 445
Cdd:COG2319   287 HSGGVNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHtgaVRSVAFSP-----DGKTLASGSDDGTVRLWDLAT 361

                          90       100
                  ....*....|....*....|....*
gi 1720373124 446 ARLLRTIPSPcptsKANIPSVAFSP 470
Cdd:COG2319   362 GELLRTLTGH----TGAVTSVAFSP 382
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 315-442 1.63e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 55.80  E-value: 1.63e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 315 TGHrqsyriNSDVLAQQFAVKTPLLFNGCRSGEIFAIDLRSPSqakgWKATQIFHDSAVTSVQVFKEEQHLMASDMSGKI 394
Cdd:cd00200   174 TGH------TGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGK----CLGTLRGHENGVNSVAFSPDGYLLASGSEDGTI 243

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1720373124 395 KLWDLRATKCVRQYEGHVNE-YAylpLHMHEEEGILVAVGQDCYTRIWS 442
Cdd:cd00200   244 RVWDLRTGECVQTLSGHTNSvTS---LAWSPDGKRLASGSADGTIRIWD 289
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 369-470 2.30e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 55.42  E-value: 2.30e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 369 HDSAVTSVQvFKEEQHLMASDMS-GKIKLWDLRATKCVRQYEGH---VNEYAYLPlhmheEEGILVAVGQDCYTRIWSLH 444
Cdd:cd00200    92 HTSYVSSVA-FSPDGRILSSSSRdKTIKVWDVETGKCLTTLRGHtdwVNSVAFSP-----DGTFVASSSQDGTIKLWDLR 165

                          90       100
                  ....*....|....*....|....*.
gi 1720373124 445 DARLLRTIPSpcptSKANIPSVAFSP 470
Cdd:cd00200   166 TGKCVATLTG----HTGEVNSVAFSP 187
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 369-470 6.49e-07

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 51.18  E-value: 6.49e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 369 HDSAVTSVQVFKEEQHLMASDMSGKIKLWDLRATKCVRQYEGH---VNEYAYLPLHMHeeegiLVAVGQDCYTRIWSLHD 445
Cdd:cd00200     8 HTGGVTCVAFSPDGKLLATGSGDGTIKVWDLETGELLRTLKGHtgpVRDVAASADGTY-----LASGSSDKTIRLWDLET 82

                          90       100
                  ....*....|....*....|....*
gi 1720373124 446 ARLLRTIPSpcptSKANIPSVAFSP 470
Cdd:cd00200    83 GECVRTLTG----HTSYVSSVAFSP 103
WD40 COG2319
WD40 repeat [General function prediction only];
338-445 5.30e-05

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 45.67  E-value: 5.30e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1720373124 338 LLFNGCRSGEIFAIDLRSPSQAKGWKAtqifHDSAVTSVQVFKEEQHLMASDMSGKIKLWDLRATKCVRQYEGH---VNE 414
Cdd:COG2319   302 LLASGSDDGTVRLWDLATGKLLRTLTG----HTGAVRSVAFSPDGKTLASGSDDGTVRLWDLATGELLRTLTGHtgaVTS 377

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1720373124 415 YAYLPlhmheEEGILVAVGQDCYTRIWSLHD 445
Cdd:COG2319   378 VAFSP-----DGRTLASGSADGTVRLWDLAT 403
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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