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Conserved domains on  [gi|1907154449|ref|XP_036019641|]
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Krueppel-like factor 4 isoform X1 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
KLF1_2_4_N super family cl41729
N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel ...
 24-350 1.63e-93

N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the related N-terminal domains of KLF1, KLF2, KLF4, and similar proteins.


The actual alignment was detected with superfamily member cd21582:

Pssm-ID: 425360 [Multi-domain]  Cd Length: 335  Bit Score: 285.05  E-value: 1.63e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449  24 SGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSpassgpasapsTCSFSYPIRA 103
Cdd:cd21582    48 CPPLGGRCYDQDPPLGMRRKEEEFNDLLDYDFILSNSLLQQQQQQQQAASTSSSTSSSS-----------SSPYSYQLPS 116

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 104 ggdpgvaASNTGGGLLYsresappPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQpqppgggLMGKFVLKASLT 183
Cdd:cd21582   117 -------PQGTATSFLY-------PIPRANLADINDVSPSGGFVAELMRPDLDPAYLQPTS-------LHGKFVVKTTMD 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 184 TpgSEYSSPSVISVSKGSPDGShpvvvAPYSGGPPRMCPKIKQEAVPSCTVSRSLEAHLsagpqlsngHRPNTHDFPLGR 263
Cdd:cd21582   176 M--GDYSQSINVSKSAPMTKSS-----VAPSSSLPFMCPRIKQENPSTCTISRPMDGHL---------GGNSQHGFSQRA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 264 QLPTRTTPTLSPEEL-------LNSRDCHPG------LPLPPGFHPHPGPNYPPFLPDQMQSQvpslHYQELMPPGSCLP 330
Cdd:cd21582   240 PLPSRTTPSGGPGGGnsstaesLMSRDHHPSsqvlshPPLPLPQGYHPSPGYPPFPPPPSQPQ----QYQELMSPGSCLP 315

                         330       340
                  ....*....|....*....|
gi 1907154449 331 EEPKPKRGRRSWPRKRTATH 350
Cdd:cd21582   316 EEPKPKRGRRSWPRKRTATH 335
zf-H2C2_2 pfam13465
Zinc-finger double domain;
396-421 1.41e-05

Zinc-finger double domain;


:

Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.59  E-value: 1.41e-05
                          10        20
                  ....*....|....*....|....*.
gi 1907154449 396 ELTRHYRKHTGHRPFQCQKCDRAFSR 421
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
345-428 1.91e-04

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 43.53  E-value: 1.91e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 345 KRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCdRAFSRSDH 424
Cdd:COG5048   382 KNDKKSETLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCSIL-KSFRRDLD 460


                  ....
gi 1907154449 425 LALH 428
Cdd:COG5048   461 LSNH 464
 
Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 24-350 1.63e-93

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 285.05  E-value: 1.63e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449  24 SGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSpassgpasapsTCSFSYPIRA 103
Cdd:cd21582    48 CPPLGGRCYDQDPPLGMRRKEEEFNDLLDYDFILSNSLLQQQQQQQQAASTSSSTSSSS-----------SSPYSYQLPS 116

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 104 ggdpgvaASNTGGGLLYsresappPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQpqppgggLMGKFVLKASLT 183
Cdd:cd21582   117 -------PQGTATSFLY-------PIPRANLADINDVSPSGGFVAELMRPDLDPAYLQPTS-------LHGKFVVKTTMD 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 184 TpgSEYSSPSVISVSKGSPDGShpvvvAPYSGGPPRMCPKIKQEAVPSCTVSRSLEAHLsagpqlsngHRPNTHDFPLGR 263
Cdd:cd21582   176 M--GDYSQSINVSKSAPMTKSS-----VAPSSSLPFMCPRIKQENPSTCTISRPMDGHL---------GGNSQHGFSQRA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 264 QLPTRTTPTLSPEEL-------LNSRDCHPG------LPLPPGFHPHPGPNYPPFLPDQMQSQvpslHYQELMPPGSCLP 330
Cdd:cd21582   240 PLPSRTTPSGGPGGGnsstaesLMSRDHHPSsqvlshPPLPLPQGYHPSPGYPPFPPPPSQPQ----QYQELMSPGSCLP 315

                         330       340
                  ....*....|....*....|
gi 1907154449 331 EEPKPKRGRRSWPRKRTATH 350
Cdd:cd21582   316 EEPKPKRGRRSWPRKRTATH 335
zf-H2C2_2 pfam13465
Zinc-finger double domain;
396-421 1.41e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.59  E-value: 1.41e-05
                          10        20
                  ....*....|....*....|....*.
gi 1907154449 396 ELTRHYRKHTGHRPFQCQKCDRAFSR 421
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
345-428 1.91e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 43.53  E-value: 1.91e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 345 KRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCdRAFSRSDH 424
Cdd:COG5048   382 KNDKKSETLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCSIL-KSFRRDLD 460


                  ....
gi 1907154449 425 LALH 428
Cdd:COG5048   461 LSNH 464
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 350-374 7.39e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 36.51  E-value: 7.39e-04
                          10        20
                  ....*....|....*....|....*
gi 1907154449 350 HTCDYagCGKTYTKSSHLKAHLRTH 374
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23
ZnF_C2H2 smart00355
zinc finger;
410-432 6.94e-03

zinc finger;


Pssm-ID: 197676  Cd Length: 23  Bit Score: 33.98  E-value: 6.94e-03
                           10        20
                   ....*....|....*....|...
gi 1907154449  410 FQCQKCDRAFSRSDHLALHMKRH 432
Cdd:smart00355   1 YRCPECGKVFKSKSALREHMRTH 23
 
Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 24-350 1.63e-93

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 285.05  E-value: 1.63e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449  24 SGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSpassgpasapsTCSFSYPIRA 103
Cdd:cd21582    48 CPPLGGRCYDQDPPLGMRRKEEEFNDLLDYDFILSNSLLQQQQQQQQAASTSSSTSSSS-----------SSPYSYQLPS 116

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 104 ggdpgvaASNTGGGLLYsresappPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQpqppgggLMGKFVLKASLT 183
Cdd:cd21582   117 -------PQGTATSFLY-------PIPRANLADINDVSPSGGFVAELMRPDLDPAYLQPTS-------LHGKFVVKTTMD 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 184 TpgSEYSSPSVISVSKGSPDGShpvvvAPYSGGPPRMCPKIKQEAVPSCTVSRSLEAHLsagpqlsngHRPNTHDFPLGR 263
Cdd:cd21582   176 M--GDYSQSINVSKSAPMTKSS-----VAPSSSLPFMCPRIKQENPSTCTISRPMDGHL---------GGNSQHGFSQRA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 264 QLPTRTTPTLSPEEL-------LNSRDCHPG------LPLPPGFHPHPGPNYPPFLPDQMQSQvpslHYQELMPPGSCLP 330
Cdd:cd21582   240 PLPSRTTPSGGPGGGnsstaesLMSRDHHPSsqvlshPPLPLPQGYHPSPGYPPFPPPPSQPQ----QYQELMSPGSCLP 315

                         330       340
                  ....*....|....*....|
gi 1907154449 331 EEPKPKRGRRSWPRKRTATH 350
Cdd:cd21582   316 EEPKPKRGRRSWPRKRTATH 335
KLF2_N cd21583
N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as ...
 39-350 1.77e-23

N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as Krueppel-like factor 2 or lung Kruppel-like Factor/LKLF) is a protein that, in humans, is encoded by the KLF2 gene on chromosome 19. It has been implicated in a variety of biochemical processes in the human body, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF2 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF2, which is related to the N-terminal domains of KLF1 and KLF4.


Pssm-ID: 409229 [Multi-domain]  Cd Length: 299  Bit Score: 99.72  E-value: 1.77e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449  39 LARRETEEFNDLLDLDFILSNSLTHQESVAATVTtsasassssspassgpasapstcsfSYPIRAGGDPGVAASNTGGGL 118
Cdd:cd21583    46 LGRKDDEDLDNYLDLDFILANTAGSDSAGAAGGG-------------------------YYGSLPEPAAPYSASNPPGAY 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 119 LYSRESAPPPtapfnladindvsPSGGFVAELLRPELDPVYippqqpqPPGGGLMGKFVLKASlttPGSEYSSPSVISVS 198
Cdd:cd21583   101 SVPEQGSPPP-------------PYSSSLMAELLRDSDSDY-------GPSNSIQGRFLVSSA---FFPRQDDPDSIKVE 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 199 kGSPDGSHPVVvapysGGPPRMCPKIKQEAVPSCTVSrsleahlsaGPQLSNGHRPnthdfplgrQLPTRTTPTLSPEEL 278
Cdd:cd21583   158 -PSMDSYGPVM-----GMVPQSCPKIKQEGNVSCMMS---------FEQPRLANSP---------QAAGSMTPPLSPDDL 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 279 LNSrDCHPGLPLPPGFHPHPGPNYPPFLPDQMQSQVPS-------------LHYQELMPPGSCLPE--EPKPKRGRRSWP 343
Cdd:cd21583   214 MAS-ECQQQMMCSPSFPQPHHPHYPPPPQPPLQYQSPHqfglfedglplqpSAQRVLLTPPSSPLEllESKPKRGRRSWP 292


                  ....*..
gi 1907154449 344 RKRTATH 350
Cdd:cd21583   293 RKRTATH 299
KLF1_2_4_N cd21972
N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel ...
 125-350 2.75e-18

N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the related N-terminal domains of KLF1, KLF2, KLF4, and similar proteins.


Pssm-ID: 409230 [Multi-domain]  Cd Length: 194  Bit Score: 82.73  E-value: 2.75e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 125 APPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQpqppggglmgkfvlKASLTTPGSEYSSPSVISVSKGSPDG 204
Cdd:cd21972    12 AKPEDDLSKFLDLEFILSNTVTSDNDNPPPPDPAYPPPES--------------PESCSTVYDSDGCHPTPNAYCGPNGP 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 205 SHPVVVApYSGGPPRMCPKIKQEAVPSCTvsrsleaHLSAGPQLSNGHRPNTHDFPlgrqlptrttptlspeellnsrdc 284
Cdd:cd21972    78 GLPGHFL-LAGNSPNLGPKIKTENQEQAC-------MPVAGYSGHYGPREPQRVPP------------------------ 125

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1907154449 285 HPGLPlppgfhphpgPNYPPFLPDQMQSQVPSLHYQ-------------ELMPPGSCLPEEPKPKRGRRSWPRKRTATH 350
Cdd:cd21972   126 APPPP----------QYAGHFQYHGHFNMFSPPLRAnhpgmstvmltplSTPPLGFLSPEEAKPKRGRRSWARKRTATH 194
KLF1_2_4_N-like cd22056
N-terminal domain of Kruppel-like factors with similarity to the N-terminal domains of ...
 50-350 2.53e-06

N-terminal domain of Kruppel-like factors with similarity to the N-terminal domains of Kruppel-like factor (KLF)1, KLF2, and KLF4; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domains of an unknown subfamily of KLFs, predominantly found in fish, related to the N-terminal domains of KLF1, KLF2, and KLF4.


Pssm-ID: 409231 [Multi-domain]  Cd Length: 339  Bit Score: 49.27  E-value: 2.53e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449  50 LLDLDFILSNSLthqesvaatvttSASASSSSSPASSGPASAPSTCSFSYPiraggdpgvaASNTGGGLLYSRESAPPPT 129
Cdd:cd22056    56 FLDEEDILSNFL------------DLEFILSSSNGSSSGPSQQQQCAYPLP----------ESPESCSTGSDSDGSDAPQ 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 130 APFNLADINDVSPSGGFVAELLRPELdpvyippQQPQPPGGGLMGKFvlkasltTPGSEYSSPSVISVskgspdgSHPVV 209
Cdd:cd22056   114 PYHGSAGFTSSSPVHSLMAELLTPEM-------NYPGESSPDSAGKA-------RDGREYTELRALPT-------APPAH 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 210 VAPYSggPPRMCPKIKQEAV-PSCTVSRSLEAH----------LSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPeel 278
Cdd:cd22056   173 QPATS--PPPLGYKIKTEPVeQSCMMAAGGGGFmgqqkpkhqmHSVHPQAFTHHQAAGPGALQGRGGRGGPDCHLLH--- 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 279 lNSRDCHPGLPLppgFHPHPGPNYPPFLPDQMQSQ--------------------------VPSLHYQELMPPGSclPEE 332
Cdd:cd22056   248 -SSHHHHHHHHL---QYQYMNAPYPPHYAHQGAPQfhgqysvfrepmrvhhqghpgsmltpPSSPPLLEFYAQDE--PED 321

                         330
                  ....*....|....*...
gi 1907154449 333 PKPKRGRRSWPRKRTATH 350
Cdd:cd22056   322 IKPKRGRRSWARKRTATH 339
zf-H2C2_2 pfam13465
Zinc-finger double domain;
396-421 1.41e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.59  E-value: 1.41e-05
                          10        20
                  ....*....|....*....|....*.
gi 1907154449 396 ELTRHYRKHTGHRPFQCQKCDRAFSR 421
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 410-432 6.13e-05

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 39.59  E-value: 6.13e-05
                          10        20
                  ....*....|....*....|...
gi 1907154449 410 FQCQKCDRAFSRSDHLALHMKRH 432
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
KLF1_N cd21581
N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as ...
 305-350 8.31e-05

N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as Krueppel-like factor 1 or Erythroid Kruppel-like Factor/EKLF) was the first Kruppel-like factor discovered. It was found to be vitally important for embryonic erythropoiesis in promoting the switch from fetal hemoglobin (Hemoglobin F) to adult hemoglobin (Hemoglobin A) gene expression by binding to highly conserved CACCC domains. EKLF ablation in mouse embryos produces a lethal anemic phenotype, causing death by embryonic day 14, and natural mutations lead to beta+ thalassemia in humans. However, expression of embryonic hemoglobin and fetal hemoglobin genes is normal in EKLF-deficient mice, suggesting other factors may be involved. KLF1 functions as a transcriptional activator. It belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF1, which is related to the N-terminal domains of KLF2 and KLF4.


Pssm-ID: 409227 [Multi-domain]  Cd Length: 278  Bit Score: 44.27  E-value: 8.31e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1907154449 305 FLPDQMQSQVPS--LHYQELMPPGSCLPEE-PKPKRGRRSWPRKRTATH 350
Cdd:cd21581   230 LFPDYAHSPGPGhlPLGQQPLLPDPPLPPGgAEGKRGRRSWAKKRPAVH 278
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
345-428 1.91e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 43.53  E-value: 1.91e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907154449 345 KRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCdRAFSRSDH 424
Cdd:COG5048   382 KNDKKSETLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCSIL-KSFRRDLD 460


                  ....
gi 1907154449 425 LALH 428
Cdd:COG5048   461 LSNH 464
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 350-374 7.39e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 36.51  E-value: 7.39e-04
                          10        20
                  ....*....|....*....|....*
gi 1907154449 350 HTCDYagCGKTYTKSSHLKAHLRTH 374
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
357-415 1.96e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 40.45  E-value: 1.96e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1907154449 357 CGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKC 415
Cdd:COG5048    39 CTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHHNNPSDLNSKS 97
zf-H2C2_2 pfam13465
Zinc-finger double domain;
366-393 2.84e-03

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 35.04  E-value: 2.84e-03
                          10        20
                  ....*....|....*....|....*...
gi 1907154449 366 HLKAHLRTHTGEKPYHCdwDGCGWKFAR 393
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKC--PECGKSFKS 26
ZnF_C2H2 smart00355
zinc finger;
410-432 6.94e-03

zinc finger;


Pssm-ID: 197676  Cd Length: 23  Bit Score: 33.98  E-value: 6.94e-03
                           10        20
                   ....*....|....*....|...
gi 1907154449  410 FQCQKCDRAFSRSDHLALHMKRH 432
Cdd:smart00355   1 YRCPECGKVFKSKSALREHMRTH 23
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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