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Xiphophorus maculatus 5.0 assembly.
 The platyfish DNA for single molecule real time (SMRT) sequencing is derived from a single male (Xiphophorus maculatus, Strain - JP 163 A) from the Xiphophorus Genetic Stock Center (Dr. Ron Walter, Director), Texas State ... University, San Marcos, Texas, USA. X. maculatus Jp 163 A are line bred (i.e., brother-sister matings) and this fish is from the 114th generation if line breeding. Sequences were generated on the Pacific Biosciences Sequel instrument (V2 chemistry) to approx. 83x genome coverage based on a genome size estimate of 700 Mb. All SMRT sequences were assembled with the HGAP4 algorithm (SMRT Link v5.0.1.9585) then error corrected using the Arrow error-correction module. Additional polishing of the assembly for residual indels was done by aligning 
50x coverage of Illumina data and the Pilon algorithm. Scaffolds were generated by alignment to a Bionano map created with the same DNA source using the Irys software. Finally, all scaffolds were ordered and oriented by alignment to the genetic linkage map using Chromonomer (http://catchenlab.life.illinois.edu/chromonomer/.cite). 
 Of the 704 Mb assembled genome (X_maculatus-5.0), the total assembly N50 contig and scaffold lengths are 9.2Mb (n=259) and 31.5Mb (n=103), respectively. 
 For questions regarding this X_maculatus-5.0 assembly please contact Dr. Wes Warren, McDonnell Genome Institute at Washington University School of Medicine, St. Louis, MO or Dr. Ron Walter, Texas State University, San Marcos, Texas, USA. 
 Data use:
 The X_maculatus-5.0 assembly sequence is made freely available to the community by McDonnell Genome Institute at Washington University School of Medicine, with the following understanding: 1. The data may be freely downloaded, used in analyses, and repackaged in databases. 2. Users are free to use the data in scientific papers analyzing these data if the providers of this data are properly cited. 3. Any redistribution of the data should carry this notice. 
 Xiphophorus maculatus Sequence and Assembly Credits:
 DNA source - Dr. Ron Walter, Texas State University, San Marcos, TX.
 Genome Sequence - The McDonnell Genome Institute, Washington University School of Medicine.
 Sequence Assembly and Chromosomal Sequence Construction - The McDonnell Genome Institute, Washington University School of Medicine
 Platyfish RNAseq data - Dr. Ron Walter, Texas State University, San Marcos, TX. 
 Funding for the sequence characterization of the platyfish genome is being provided by grants to Dr. Wesley Warren, McDonnell Genome Institute, Washington University Schoold of Medicine and Dr. Ron Walter through the National Institutes of Health (NIH) and Dr. Manfred Schartl at the Universitat Wurzburg, Germany.

 ASSEMBLY STATS:
 SCAFFOLDS
 COUNT 103 
 LENGTH 704,304,639 bp 
 AVG 6,837,909 bp 
 N50 31,535,491 bp
 LARGEST 35,293,739 bp 
 Scaffolds > 1M: 24 ( 699,980,690 bp ) 99.4%
 Scaffolds 250K--1M: 1 ( 269,636 bp ) 0.04%
 Scaffolds 100K--250K: 14 ( 1,854,102 bp ) 0.26%
 Scaffolds 10K--100K: 52 ( 2,150,311 bp ) 0.31%
 Scaffolds 5K--10K: 5 ( 37,383 bp ) 0.005%
 Scaffolds 2K--5K: 3 ( 9,783 bp ) 0.001%
 Scaffolds 0--2K: 4 ( 2,734 bp ) 0.0004%
 CONTIGS
 COUNT 259 
 LENGTH 700,976,734 bp 
 AVG 2,706,473 bp 
 N50 9,181,372 bp
 LARGEST 26,812,185 bp 
 Contigs > 1M: 115 ( 664,204,175 bp ) 94.8%
 Contigs 250K--1M: 54 ( 30,725,410 bp ) 4.4%
 Contigs 100K--250K: 26 ( 3,846,938 bp ) 0.55%
 Contigs 10K--100K: 52 ( 2,150,311 bp ) 0.31%
 Contigs 5K--10K: 5 ( 37,383 bp ) 0.005%
 Contigs 2K--5K: 3 ( 9,783 bp ) 0.001%
 Contigs 0--2K: 4 ( 2,734 bp ) 0.0004%  more

Global statistics