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IDs: 6467848 [UID] 6467848 [GenBank]
One of a set of three complete genomes from a study linking the HIV-1 epidemic in the heterosexual population in Thailand to an A/E recombinant. 90cr402, previously named CAR-E 4002, was obtained from a man from Bangui, Central African ... Republic, who had lymphadeopathy, diarrhea, severe weight loss and recurrent respiratory infections. He was infected through heterosexual contact, but the year of infection is unknown. 90cr102, GenBank accession number U51188, was first adapted to growth in chimpanzee cells, expanded in chimpanzee cells, and then re-expanded in human PBMCs before lamba cloning and sequencing. 93th253, U51189, is from a 21-year-old man from Chiang Mai, Thailand and was previously named CMU010 or 302053. The patient had end-stage AIDS. The mode and year of infection are unknown. 93th253 was isolated and expanded in human PBMCs, then expanded in H9 cells, and followed by lambda cloning and sequencing. 92ug037 was obtained from WHO-NIAIDS and comes from an asymptomatic 31-year-old female from Entebbe, Uganda, early in infection. The year of infection is unknown and the mode of infection was heterosexual contact. 92ug037, U51190, was established and propagated by cocultivation with normal donor lymphocytes, and them PCR amplified and sequenced. 93th253 and 90cr402 were at first believed to be subtype E, but were later determined to be A/E recombinants. Furthermore, comparison of the two strains showed that they were derived from a common A/E recombinant ancestor, presumably from Central Africa. 92ug037 is subtype A. LTR sequences are available under accession numbers U51282-U51297. more
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