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PDT000860053.1

Description:
NCBI Pathogen Detection Assembly PDT000860053.1
Organism name:
Escherichia coli (E. coli)
Taxonomy check:
OK
Infraspecific name:
Strain: NCCPS_66
BioSample:
SAMN16403707
BioProject:
PRJNA293225
Submitter:
US Food and Drug Administration
Date:
2020/10/22
Assembly type:
na
Assembly level:
Contig
Genome representation:
full
Excluded from RefSeq:
  • from large multi-isolate project
GenBank assembly accession:
GCA_014912525.1 (latest)
RefSeq assembly accession:
n/a
RefSeq assembly and GenBank assembly identical:
n/a
WGS Project:
AAWMAJ01
Assembly method:
SKESA v. 2.2
Expected final version:
no
Genome coverage:
53x
Sequencing technology:
ILLUMINA

IDs: 8256611 [UID] 22552938 [GenBank]

See Genome Information for Escherichia coli

There are 266030 assemblies for this organism

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History (Show revision history)

Comment

The annotation was added by the assembly submitters using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
This draft WGS assembly was generated by running SKESA to generate a de-novo assembly. The ... de-novo assembly was then concatenated with contigs generated using a guided assembler using antimicrobial resistance genes as baits to comprehensively catalog the set of resistance genes in the isolate. Note, some parts of the contigs derived from the guided assembler may overlap de-novo contigs, and other guided assembler contigs. De-novo contigs can be differentiated from guided assembler contigs by their names , which include either 'denovo' or 'guided'.  more

Genome-Annotation-Data

##Genome-Annotation-Data-START##
Annotation Date::10/12/2020 23:09:14
Annotation Method::Best-placed reference protein set; GeneMarkS-2+
Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline (PGAP)
Annotation Provider::NCBI
Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
Annotation Software revision::2020-02-06.build4373
Genes (total)::4,537
CDSs (total)::4,445
Genes (coding)::4,379
CDSs (with protein)::4,379
Genes (RNA)::92
rRNAs::5, 2, 2 (5S, 16S, 23S)
complete rRNAs::5 (5S)
partial rRNAs::2, 2 (16S, 23S)
tRNAs::73
ncRNAs::10
Pseudo Genes (total)::66
CDSs (without protein)::66
Pseudo Genes (ambiguous residues)::0 of 66
Pseudo Genes (frameshifted)::31 of 66
Pseudo Genes (incomplete)::27 of 66
Pseudo Genes (internal stop)::22 of 66
Pseudo Genes (multiple problems)::13 of 66
CRISPR Arrays::2
##Genome-Annotation-Data-END##

Global statistics

Total sequence length4,723,019
Total ungapped length4,723,019
Number of contigs41
Contig N50246,193
Contig L506
Total number of chromosomes and plasmids0
Number of component sequences (WGS or clone)41

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Global assembly definition

Download the full sequence report
Click on the table row to see sequence details in the table to the right
Assembly Unit Name
Primary Assembly
The primary assembly unit does not have any assembled chromosomes or linkage groups.
Please download the full sequence report for information on the scaffolds.

Assembly statistics

MoleculeTotal
Length
Contig
Count
Ungapped
Length
Contig
N50
Spanned
Gaps
Unspanned
Gaps
unplaced4,723,019414,723,019246,19300

Assembly QA

Taxonomy Check Data

Declared organism

Organism nameSpecies name
Escherichia coliEscherichia coli

Best-matching type-strain assembly for declared species

AssemblyOrganism nameType category
GCA_000010385.1Escherichia coli SE11claderef

Best-matching type-strain assembly

AssemblySpecies nameType category
GCA_000010385.1Escherichia colicladeref

Average Nucleotide Identity (ANI) data

ANIQuery coverageSubject coverage
Declared type99.2993.9786.09
Best-match type99.2993.9786.09

ANI result

Taxonomy check statusBest match statusComment
OKspecies-matchna