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We sequenced the hermaphroditic freshwater snail, Biomphalaria glabrata (strain BB02), the host for the medically important trematode parasite, Schistosoma mansoni, using 1X coverage from plasmids and 0.1X BAC end sequencing on the ABI3730xl and 10X coverage sequenced on the ... Roche 454 Sequencer (including 2 paired end read runs) was initially assembled with Newbler. The Newbler assembly was screened for contamination then merged with a Soap assembly of Illumina reads followed by use of an in-house program for collapsing of redundant heterozygous contigs. Next, we applied our program, GapCloser, which closes gaps in the assembly by making iterative joins using Illumina reads. Finally we removed contigs less than 200 bases and incorporated reads into the assembly that had been assembled in a prior version of the Newbler assembly but were not assembled in this round. The resulting assembly, going by the name of assembly version 4.3, is 898.9Mb bases with an N50 contig length of 6.9kb and N50 scaffold length of 42kb.
 For creation of the linkage group AGP files, we identified all scaffolds that were uniquely placed on a single linkage group. Because of low marker density, only 145Mb was localized to specific linkage groups and scaffolds could not be ordered and oriented within linkage groups. Therefore, the scaffolds were simply placed in the order suggested by the linkage mapping on *_random for each linkage group.
 Credits:
 B. glabrata BB02 samples - Omar dos Santos Carvalho, Centro de Pesquisas Rene Rachou-Fiocruz (sample location: Barreiro, Brazil) BAC library - Coen M. Adema et al. (2006) Sequencing and Assembly - The Genome Institute, Washington University School of Medicine Linkage map - Jacob Tennessen and Michael Blouin, Oregon State University White Paper - Matty Knight et al (2003) Others -
 - Fred Lewis Biomedical Research Institute of the American Foundation of Biomedical Research
 - Eric Loker University of New Mexico Biology
 - Nithya Raghavan Biomedical Research Institute of the American Foundation of Biomedical Research
In February 2023 1397 sequences were suppressed because they were found to be contaminants.  more

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