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IDs: 182621 [UID] 1104068 [GenBank] 1174948 [RefSeq]
The DNA used for sequencing the Malayan flying lemur, Galeopterus variegatus, was derived from a single male animal collected in West Java by Minoru Baba (Kitakyushu Museum of Natural History and Human History,Japan) under Indonesian Institute of Sciences Research ... Permits 6541/I/KS/1999, 3452/SU/KS/2002, and 3380/SU/KS/2003. Ethanol preserved tissue was used to extract genomic DNA in the lab of William Murphy by Victor Mason (Texas A&M University). Total input sequence coverage of Illumina reads was 60X (45X 230-330 short inserts, 15X 3kbs, and 5X 8kbs) using a genome size estimate of 3.0Gb. The assembled sequence coverage was 55X. The combined sequence reads were assembled using the SOAPdenovo2 software (BGI). The assembly was improved using an unpublished program designed to close gaps, and SSPACE (Boetzer 2010). This draft assembly was referred to as Galeopterus variegatus 3.0.2. This version has been gap filled, error-corrected with approx. 12X Illumina reads, and cleaned of contaminating contigs. The assembly is made up of a total of 179,513 scaffolds with an N50 scaffold length of 249kb (N50 contig length was 20.8kb). The assembly spans over 2.9Gb. This work was funded by NIH grant HG003079 to RKW, The Genome Institute at Washington University School of Medicine. For questions regarding this Galeopterus variegatus 3.0.2 assembly please contact Wes Warren, Washington University School of Medicine (wwarren@genome.wustl.edu). Funding for the sequence characterization of the Galeopterus variegatus genome was provided by the Office of Director, National Institutes of Health (NIH). *************************************************************************** Sequence and Assembly Credits: Sequencing DNA source credit: - William Murphy DNA source can be obtained from: William Murphy, VIBS Texas A&M University 4458 TAMU College Station, TX 77843-4458 Genome Sequence - The Genome Institute, Washington University School of Medicine Sequence Assembly - The Genome Institute, Washington University School of Medicine Citation upon use of this assembly in a manuscript: It is requested that users of this Galeopterus variegatus 3.0.2 sequence assembly acknowledge Richard K. Wilson and The Genome Institute, Washington University School of Medicine in any publications that result from use of this sequence assembly. ******************************************************************* Assembly Stats: *** Contiguity: Contig *** Total contig number: 511879 Total contig bases: 2802903715 bp Average contig length: 5476 bp Maximum contig length: 521467 bp N50 contig length: 20753 bp N50 contig number: 33045 *** Contiguity: Supercontig *** Total supercontig number: 179513 Average supercontig length: 15614 bp Maximum supercontig length: 3249419 bp N50 supercontig length: 249067 bp N50 supercontig number: 3001 *** Scaffold Distribution *** Scaffolds > 1M: 137 Scaffold 250K--1M: 2843 Scaffold 100K--250K: 4525 Scaffold 10--100K: 12393 Scaffold 5--10K: 6621 Scaffold 2--5K: 17944 Scaffold 0--2K: 135050 more
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