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Poeciliia mexicana (Shortfin molly) Sequence Assembly Release Notes
 The Shortfin molly DNA for shotgun sequencing is derived from a adult female (Poecilia mexicana; fish id # Pme_1353_1.1) within the laboratory of Dr. Manfred Schartl at the Biocenter of the ... University of Wuerzburg, Germany. Total sequence genome input coverage on the Illumina HiSeq instrument was 
30x (22x fragments, and 8x 3kb).
 The combined sequence reads were assembled into contigs by Richa Agarwala by aligning the reads to the Poecilia formosa draft assembly. The P. mexicana assembly was further improved with the external scaffolding tool SSPACE (Boetzer 2010), and with a gap filling tool similar to IMAGE (Tsai 2010). This 1.0 version has been cleaned of contaminating contigs, and contigs 200bp and less were removed. The assembly is made up of a total of 18,105 scaffolds (including single contig scaffolds) with an N50 scaffold length of almost 254Mb (N50 contig length was almost 40kb). The assembly spans 803Mb. 
 This work was supported by NIH grant R24 RR032658-01 to Dr. Warren, Washington University School of Medicine.
 DNA Source Contact: Dr. Manfred Schartl, Physiological Chemistry I University of Wuerzburg Biozentrum, Am Hubland Wuerzburg 97074 Germany

 Poecillia mexicana 1.0 Sequence and Assembly Credits:
 DNA source - Dr. Manfred Schartl, Physiologische Chemie, Biozentrum, Am Hubland, Universitat Wuerzburg, Germany. Genome Sequence - McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO. Sequence Assembly - Richa Agarwala, National Center for Biotechnology Information, NIH, Bethesda, MD, USA, McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO. Assembly curation - Pat Minx, McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO.
 Citation upon use of this assembly in a manuscript:
 It is requested that users of this Poecillia mexicana 1.0 sequence assembly acknowledge Wesley Warren and McDonnell Genome Institute, Washington University School of Medicine in any publications that result from use of this sequence assembly. 

 Poecillia mexicana 1.0 assembly statistics: (scaffold = supercontig)
 *** Contiguity: Contig *** Total contig number: 50601 Total contig bases: 679832124 bp Average contig length: 13435 bp Maximum contig length: 293941 bp N50 contig length: 39840 bp N50 contig number: 4911
 *** Contiguity: Supercontig *** Total supercontig number: 18105 Average supercontig length: 37549 bp Maximum supercontig length: 1368293 bp N50 supercontig length: 253718 bp N50 supercontig number: 813
 *** Scaffold Distribution *** Scaffolds > 1M: 11 Scaffold 250K--1M: 821 Scaffold 100K--250K: 1207 Scaffold 10--100K: 3067 Scaffold 5--10K: 806 Scaffold 2--5K: 1313 Scaffold 0--2K: 10880  more

Global statistics