Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Download
IDs: 1982541 [UID] 7460078 [GenBank] 8351018 [RefSeq]
The Northern platyfish DNA for shotgun sequencing is derived from a single female of Xiphophorus couchianus, Pedigree - Xc77(B), from the Xiphophorus Genetic Stock Center (XGSC). For further information see: http://www.xiphophorus.txstate.edu/. The fish utilized for DNA sequencing is from ... the 77th inbred generation (sibling line bred) derived from a X. couchianus line originally collected from the Huasteca Canyon, Nuevo Leon, Mexico in 1961. Fish from this line are bred and maintained and can be obtained at the XGSC. Sequences were generated on the Pacific Biosciences Sequel instrument (V2 chemistry) to approx. 77x genome coverage based on a genome size estimate of 700 Mb. All SMRT sequences were assembled with the HGAP4 algorithm (SMRT Link v5.0.1.9585) then error corrected using the Arrow error-correction module. Additional polishing of the assembly for indels was done by aligning 50x coverage of Illumina data using Pilon. Scaffolds were generated by alignment to a Bionano map created with the same DNA source using the Irys software. Finally, all scaffolds were ordered and oriented by alignment to the X. maculatus 5.0-male assembly using Medusa. For questions regarding the use of the X_couchianus assembly please contact Dr. Wes Warren, McDonnell Genome Institute at Washington University School of Medicine, St. Louis, MO or Dr. Ron Walter, Texas State University, San Marcos, Texas, USA. Data use: The X_couchianus assembly sequence is made available to the community by McDonnell Genome Institute at Washington University School of Medicine. more
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on