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Organizing biological data
High-throughput sequencing technologies have greatly increased the poser of human genome variability studies. More...
High-throughput sequencing technologies have greatly increased the poser of human genome variability studies. We have prepared multiple paired end libraries with insert sizes ranging from 500 bp to 10 kb and fragment libraries with average insert size of 60-100bp using the NA18507 DNA belonging to a Yoruba individual. These libraries were extensively sequenced using SOLiD (ABI's next generation sequencing platform). The deep sequence coverage obtained from these diverse libraries, not only helps identify SNPs in this genome but also submicroscopic structural variations like insertions/deletions. In this high density genome re-sequencing effort we address the challenges of constructing multiple sized mate pair libraries of complex genomes. Less...
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