BioProject

The CRISPR/Cas9 technology is the most effective gene editing system. The mutagenesis efficiency critically depends on the sgRNA used. More...

The CRISPR/Cas9 technology is the most effective gene editing system. The mutagenesis efficiency critically depends on the sgRNA used. In this project, we established a reliable methods for assessing the sgRNA activity in foxtail millet protoplasts. We designed two sgRNAs to target each of the five endogenous genes, foxtail millet bran protein (SiFMBP), DNA-binding with one finger 4 (SiDof4), betaine aldehyde dehydrogenase 2 (SiBADH2) genes, granule bound starch synthase 1 (SiGBSS1) and inositol-pentakisphosphate 2-kinase 1 (SiIPK1). The mutagenesis frequency and pattern was analyzed. We also tested three multiplex genome editing systems, including MCTU, Csy4 and tRNA systems and assessed their effective in multiplex genome editing simutaneously. Less...