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Genome Information for Homo sapiens
The detection and identification of single nucleotide polymorphism (SNP) is essential for determining patient disease susceptibility and the delivery of medicines targeted to the individual.
More...The detection and identification of single nucleotide polymorphism (SNP) is essential for determining patient disease susceptibility and the delivery of medicines targeted to the individual. At present, SNP genotyping technology includes Sanger sequencing, TaqMan-probe quantitative polymerase chain reaction (qPCR), amplification-refractory mutation system (ARMS)-PCR, and Kompetitive Allele-Specific PCR (KASP). However, these technologies have some disadvantages: the high cost of development and detection, long and time consuming protocols, and high false positive rates. Focusing on these limitations, we proposed a new SNP detection method named universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR). In this method, only two types of fluorescence-labeled probes were used for SNP genotyping, thus greatly reducing the cost of development and detection for SNP genotyping. This novel approach is both cost effective and highly accurate; it is a reliable SNP genotyping method that would serve the needs of the clinician in the provision of targeted medicine.
Less...Accession | PRJNA766172 |
Data Type | Raw sequence reads, Other |
Scope | Monoisolate |
Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
Grants | - "The study of cardiovascular disease" (Grant ID 31430041, National Natural Science Foundation of China)
- "Mechanism of noncoding RNA in cardiovascular disease" (Grant ID 91849209, Major Research Program of the National Natural Science Foundation of China)
- "Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping" (Grant ID 2018M632613, China Postdoctoral Science Foundation)
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Submission | Registration date: 25-Sep-2021 Weifang Medical University |
Relevance | Medical |
Locus Tag Prefix | LC818 |
Project Data:
Resource Name | Number of Links |
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BioSample | 1 |
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