BioProject

Background: This study aimed to assess the effects of chronic sleep deprivation (CSD) on bone metabolism in growing rats and the likely underlying mechanism. More...

Background: This study aimed to assess the effects of chronic sleep deprivation (CSD) on bone metabolism in growing rats and the likely underlying mechanism. Methods: Twenty 5-week-old male Wistar rats and randomly divided into the CSD and normal control (NC) groups after one-week acclimatization. After a 6-week intervention of sleep deprivation, the left distal femurs of both groups were harvested for micro-computed tomography scans and histological analysis. Meanwhile, the right femur were measured the mRNA and protein expression via RNA sequencing and western blot. Serum bone turnover markers were evaluated at 0, 2, 4, and 6 weeks. Result: CSD impaired the bone growth, showing an imbalance of bone turnover status, dysphasia in the metaphysis growth plate, and deterioration of bone microarchitecture. Further, CSD suppressed bone formation, showing that the expression of osteogenesis-related proteins (col1α1 and osteocalcin) and mRNA (igf1, bglap, runx2, col1α1, pth1r) are down-regulated. Meanwhile, the potential of osteoblast differentiation of bone marrow-derived mesenchymal stem cells decreased in the CSD group. Differentially expressed genes were detected, and functional enrichment analyses revealed that the PI3K/AKT pathway was significantly down-regulated in the CSD group, which was further verified by the protein expression levels.Conclusion: These results suggest that CSD can significantly impaired bone health, and it may exert these effects in part by suppressing bone formation and osteoblast differentiation, and inactivating the PI3K/AKT signaling pathway. Overall design: bone mRNA profiles of 12-week Wistar rats from normal control group and chronic sleep deprivation Less...