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Organizing biological data
869 patients diagnosed with colorectal cancer (CRC) in Fudan University Shanghai Cancer Center (FUSCC) were enrolled in our study from 1st January 2020 to 31th June 2021. More...
869 patients diagnosed with colorectal cancer (CRC) in Fudan University Shanghai Cancer Center (FUSCC) were enrolled in our study from 1st January 2020 to 31th June 2021. Tissues collected by colonoscopy, puncture or surgical excision were pathologically validated, and special pathologic types were excluded. All samples were approved and authorized by the FUSCC Ethics Committee, and all participants signed informed consent.
FUSCC Panel Sequencing and Genomic Analysis
FUSCC colorectal cancer (FUSCC-CRC) panel consisted of 887 cancer-related genes, including clinically actionable targets and frequently altered genes in CRC. Sequencing of the FUSCC-CRC panel was performed as previously described by Lang et.al, Nature Communications 2020 (PMID: 33173047 ). Briefly, DNA from tumors and matched blood was fragmented, purified, end-repaired, ployA tailing and ligated to construct libraries that were pooled and sequenced using the Illumina NovaSeq 6000. Less...
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