To improve the efficiency of homology-directed repair of double-stranded breaks introduced by CRISPR-Cas9, a Cas9-PRDM9 (PR/SET Domain 9) fusion protein was generated and tested in HEK-293T cells. RNA-seq was used to quantify gene expression in the parental HEK-293T cell line.
| Accession | PRJNA866532 |
| Data Type | Raw sequence reads |
| Scope | Multispecies |
| Publications | Chen E et al., "Decorating chromatin for enhanced genome editing using CRISPR-Cas9.", Proc Natl Acad Sci U S A, 2022 Dec 6;119(49):e2204259119 |
| Submission | Registration date: 5-Aug-2022
University of California Berkeley |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 1 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 1 |