BioProject

DNA damage in the form of abasic sites, chemically altered nucleotides, and strand fragmentation, is an important limitation in obtaining genetic information from many ancient and forensic samples. More...

DNA damage in the form of abasic sites, chemically altered nucleotides, and strand fragmentation, is an important limitation in obtaining genetic information from many ancient and forensic samples. By incubating degraded DNA with specific enzyme combinations adopted from these pathways, it is possible to reverse some of the post-mortem nucleic damage prior to downstream analyses, such as library preparation, targeted enrichment and high-throughput sequencing. This study evaluates the performance of two available repair protocols, PreCR® Repair Mix and Nelson repair, on previously characterized DNA extracts from four mammoths. Less...