BioProject

Lean male mice were fed a high fat diet (HFD, lard 24% w/w) for 16 weeks. At 9 weeks, when all hallmarks of prediabetes were established, groups of mice were treated with drug (rosiglitazone, pioglitazone, T0901317, or salicylate) for another 7 weeks together with the high fat diet. More...

Lean male mice were fed a high fat diet (HFD, lard 24% w/w) for 16 weeks. At 9 weeks, when all hallmarks of prediabetes were established, groups of mice were treated with drug (rosiglitazone, pioglitazone, T0901317, or salicylate) for another 7 weeks together with the high fat diet. An additional group was switched back to a chow diet (dietary lifestyle intervention) after the first 9 weeks of high fat diet. All groups were compared to a control group receiving HFD alone and to a reference group fed chow (baseline reference) for the entire experimental period (16 weeks). Overall design: One group remained on maintenance chow throughout the entire study period (16 weeks) and served as healthy, age-matched control (n=9, samples hybridized: 7 for gonadal, 7 for subcutaneous, 8 for visceral). After the nine week run-in period, the HFD fed mice were matched into thirteen groups based on body weight. The first group was sacrificed immediately after matching (n=9, samples hybridized: 8 for gonadal, 8 for gonadal, 6 for visceral). The second group was continued on HFD until the end of the experiment at t=16 weeks (n=15, samples hybridized: 8 for gonadal, 7 for subcutaneous, 6 for visceral). The fourth group was switched to regular chow (dietary lifestyle intervention, n=9, samples hybridized: 8 for gonadal, 8 for subcutaneous, 7 for visceral). The other groups continued on HFD supplemented with drugs typically used in clinical practice. More specifically, following drugs were mixed into HFD: rosiglitazone (0.010% w/w, n=9, samples hybridized: 8 for all adipose depots), pioglitazone (0.010% w/w, n=9, samples hybridized: 8 for gonadal, 8 for subcutaneous, 6 for visceral), T0901317 (0.010% w/w, n=9, samples hybridized: 7 for all adipose depots) and salicylate (0.40% w/w, n=9, samples hybridized: 8 for all adipose depots). Less...