BioProject

AICAR is a metabolite with anti-tumoral properties. Its monophosphate derivative ZMP is an AMP mimetic activator of the protein kinases AMPK. More...

AICAR is a metabolite with anti-tumoral properties. Its monophosphate derivative ZMP is an AMP mimetic activator of the protein kinases AMPK. However, AICAR also mediates AMPK-independent effects. A kinetic transcriptome analysis was therefore carried out in Ampkα1/α2 double knockout murine embryonic fibroblasts in response to AICAR in order to unveil these AMPK-independent functions, Overall design: Ampkα1/α2 double knockout murine embryonic cells were incubated with 1 mM AICAR for 0, 1, 2, 4, 8, 12 and 24h. RNA extraction and hybridization on Affymetrix (Mouse MG-430 PM Array Strip Kit) were then performed. Analyses were performed in triplicates (except t=8h in duplicate). The fold change in gene expression corresponds to the ratio between each incubation times with AICAR and control (t=0) without AICAR. Less...