BioProject

To investigate the mechanism underlying the effect of Brn2-loss we extracted mRNA from Braf-Pten-Brn2-wt, Braf-Pten-Brn2-het, and Braf-Pten-Brn2-hom mouse melanomas and performed microarray-based transcriptome analysis. More...

To investigate the mechanism underlying the effect of Brn2-loss we extracted mRNA from Braf-Pten-Brn2-wt, Braf-Pten-Brn2-het, and Braf-Pten-Brn2-hom mouse melanomas and performed microarray-based transcriptome analysis. Overall design: We studied the effect of Brn2 loss on in vivo melanomagenesis by generating the mouse lines Tyr::CreERt2/°; BrafV600E/+; Brn2F/+ (Braf-Brn2-het), Tyr::CreERt2/°; BrafV600E/+; Brn2F/F (Braf-Brn2-hom), Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/+ (Braf-Pten-Brn2-het) and Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/F (Braf-Pten-Brn2-hom) by introducing the floxed Brn2 locus into the genome by appropriate crossings. Cre-mediated defloxing of Braf, Pten, and Brn2 loci was induced by topical application of tamoxifen during the first three days after birth. All mice were monitored for the number of tumors/mice, and the growth rate of the first tumor. Less...