BioProject

We examined phytotoxic potential of diploid potato hybrids from 15-1 population. More...

We examined phytotoxic potential of diploid potato hybrids from 15-1 population. Individuals were bulked in bulks C and D. Both bulks characterized low total glycoalkaloids content and differ phytotoxic potential. Water extract prepared from leaves of individuals of bulk C inhibited growth of test plant - mustard in 40%, while in bulk D no inhibition was observed. To evaluate cause of expression of phytotoxic potential in bulk C, we examined glycoalklaoids composition in leaf water extract (mass spectrometry), compared gene (deep RNA sequencing - BGISEQ) and protein expression (nano-LC-MS-MS/MS) profiles between the bulks. Bulks differed in glycoalkaloids composition, bulk C contained alpha-solasonine which wasn't detected in bulk D. We concluded, alpha-solasonine may act together with alpha-solamargine and increases phytotoxic potential of leave extract of bulk C. Comparison of gene expression profiles indicating on contribution of genes, not directly involved in glycoalkaloids biosynthesis to express phytotoxic potential of C bulk. We have found 3 proteins characteristic for C bulk and 2 proteins characteristic for D bulk. We found one protein threonine dehydratase biosynthetic, which is more abundant in D bulk and his abundance corresponds with gene transcripts found in RNA-seq experiment. Overall design: Examination of potato phytotoxic potential under low glycoalkaloids content. Less...