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Accession: PRJNA97665 ID: 97665

Rattus norvegicus (Norway rat)

Dietary exposure to soy or whey proteins alters colonic global gene expression profiles during rat colon tumorigenesis

See Genome Information for Rattus norvegicus
We previously reported that lifetime consumption of soy proteins or whey proteins reduced the incidence of azoxymethane (AOM)-induced colon tumors in rats. To obtain insights into these effects, global gene expression profiles of colons from rats with lifetime ingestion of casein (CAS, control diet), soy protein isolate (SPI), and whey protein hydrolysate (WPH) diets were determined. We identified 31 induced and 49 repressed genes in the proximal colons of the SPI-fed group and 44 induced and 119 repressed genes in the proximal colons of the WPH-fed group, relative to CAS. Hierarchical clustering identified the co-induction or co-repression of multiple genes by SPI and WPH. The differential expression of I-FABP (2.92-, 3.97-fold down-regulated in SPI and WPH fed rats; P = 0.023, P = 0.01, respectively), cyclin D1 (1.61-, 2.42-fold down-regulated in SPI and WPH fed rats; P = 0.033, P = 0.001, respectively), and the c-neu proto-oncogene (2.46-, 4.10-fold down-regulated in SPI and WPH fed rats; P < 0.001, P < 0.001, respectively) mRNAs were confirmed by real-time quantitative RT-PCR. SPI and WPH affected colonic neuro-endocrine gene expression: peptide YY (PYY) and glucagon mRNAs were down-regulated in WPH fed rats, whereas somatostatin mRNA and corresponding circulating protein levels, were enhanced by SPI and WPH. The identification of transcripts co- or differentially-regulated by SPI and WPH diets suggests common as well as unique anti-tumorigenesis mechanisms of action which may involve growth factor, neuroendocrine and immune system genes. SPI and WPH induction of somatostatin, a known anti-proliferative agent for colon cancer cells, would inhibit tumorigenesis Keywords: Comarative genomic hybridization Overall design: Male Sprague Dawley rats, fed one of the three purified diets, were studied at 40 weeks after AOM injection and when tumors had developed in some animals of each group. Total RNA, purified from non-tumor tissue within the proximal half of each colon, was used to prepare biotinylated probes, which were hybridized to Affymetrix RG_U34A rat microarrays.
AccessionPRJNA97665; GEO: GSE6102
Data TypeTranscriptome or Gene expression
ScopeMultiisolate
OrganismRattus norvegicus[Taxonomy ID: 10116]
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus; Rattus norvegicus
PublicationsXiao R et al., "Dietary exposure to soy or whey proteins alters colonic global gene expression profiles during rat colon tumorigenesis.", Mol Cancer, 2005 Jan 11;4(1):1
SubmissionRegistration date: 26-Oct-2006
Nutrition Center, Arkansas Chidren's Hospital
RelevanceModel Organism
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PubMed1
PMC1
Other datasets
GEO DataSets2
GEO Data Details
ParameterValue
Data volume, Spots79191
Data volume, Processed Mbytes2
Data volume, Supplementary Mbytes18

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    Dietary exposure to soy or whey proteins alters colonic global gene expression profiles during rat colon tumorigenesis
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