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AutoRELACS: Automated Generation And Analysis of Ultra-parallel ChIP-seq

(Submitter supplied) We present AutoRELACS, an automated implementation of the RELACS protocol using the Biomek i7 automated workstation (Beckman&Coulter). We test the performance of AutoRELACS by assessing 1) the scalability of the chromatin barcode integration step, 2) the quality of the generated data in comparison to the benchmark set by the manual protocol, and 3) the sensitivity of the automated method when working with low (≤ 25.000 cells/sample) and very low (≤ 5.000 cells/sample) cell numbers.
Organism:
Homo sapiens; Drosophila melanogaster
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL24676 GPL25244
258 Samples
Download data: BW
Series
Accession:
GSE147042
ID:
200147042

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