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Accession: PRJNA122465 ID: 122465

Mus musculus (house mouse)

Prkar1a haploinsufficiency leads to an overall increase in tumors caused by other genetic defects or chemical induction

See Genome Information for Mus musculus
[original title] Prkar1a haploinsufficiency in mice leads to an overall increase in tumors caused by other genetic defects or chemical induction We investigated the Prkar1a+/- mice when bred within the Rb1+/- or Trp53+/- genetic backgrounds, or treated with a 2-step skin carcinogenesis protocol. Prkar1a+/-Trp53+/- mice developed more bone sarcomas than Trp53+/- mice (p<0.05) and Prkar1a+/-Rb1+/- mice grew more and larger pituitary and thyroid tumors than Rb1+/- mice. All mice with double heterozygosity had significantly reduced life-spans compared with their single-heterozygous counterparts. Finally, Prkar1a+/- mice developed more papillomas than wild-type animals when treated with a 2-step skin carcinogenesis protocol. A whole-genome transcriptome profiling of tumors produced by all three models identified Wnt signaling as the main pathway activated by abnormal cAMP signaling in these tissues, along with (expected) cell cycle gene abnormalities, all confirmed by qRT-PCR array and immunohistochemical analyses. Overall design: Total RNA obtained from skin papillomas from Prkar1a+/- mice were compared to those obtained from WT mice. Total RNA obtained from pituitary and thyroid tumors from Rb1+/- mice were compared to those samples obtained from Prkar1a+/- Rb1+/- mice. Total RNA obtained from sarcomas from Tp53+/- mice were compared to those samples obtained from Prkar1a+/- Tp53+/- mice.
AccessionPRJNA122465; GEO: GSE19576
Data TypeTranscriptome or Gene expression
ScopeMultiisolate
OrganismMus musculus[Taxonomy ID: 10090]
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus; Mus musculus
PublicationsAlmeida MQ et al., "Mouse Prkar1a haploinsufficiency leads to an increase in tumors in the Trp53+/- or Rb1+/- backgrounds and chemically induced skin papillomas by dysregulation of the cell cycle and Wnt signaling.", Hum Mol Genet, 2010 Apr 15;19(8):1387-98
SubmissionRegistration date: 24-Dec-2009
Dr. Stratakis Lab, Section on Genetics and Endocrinology / NICHD, NIH
RelevanceModel Organism
Project Data:
Resource NameNumber
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Publications
PubMed1
PMC1
Other datasets
GEO DataSets1
GEO Data Details
ParameterValue
Data volume, Spots591031
Data volume, Processed Mbytes16
Data volume, Supplementary Mbytes2

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