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SLX-19432.i729_i505.HK3H3BBXY.s_2

Identifiers
BioSample: SAMEA14418499; Sample name: E-MTAB-11359:SLX-19432.i729_i505.HK3H3BBXY.s_2; SRA: ERS12024061
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
isolatenot applicable
age74 to 80 week
genotypeSema4A -/-
tissuebone marrow
phenotypeaged
cell typehematopoietic stem cell
organismMus musculus
immunophenotypeLinâˆ' Sca-1+ cKit+
protocolSmart-seq2
single cell well qualityOK
inferred cell typehematopoietic stem cell
ENA-FIRST-PUBLIC2022-05-20
ENA-LAST-UPDATE2022-05-20
Description

Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

BioProject
PRJEB52992 Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence
Retrieve all samples from this project

Submission
EBI; 2022-05-21
Accession:
SAMEA14418499
ID:
28576224

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