Metagenome-Assembled Genome: ERR9968821_bin.23_Met... - BioSample

Metagenome-Assembled Genome: ERR9968821_bin.23_MetaWRAP_v1.3_MAG

Identifiers: BioSample: SAMEA114444320; SRA: ERS16432104

Organism: Actinomyces oris
cellular organisms; Bacteria; Terrabacteria group; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae; Actinomyces

Attributes

broad-scale environmental context	human digestive system
collection date	2015-04-08
environmental medium	plaque biofilm
geographic location	Australia
investigation type	metagenome-assembled genome
isolation source	oral metagenome
project name	Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.
sample name	ERR9968821_bin.23_MetaWRAP_v1.3_MAG
ENA-CHECKLIST	ERC000047
INSDC center name	EMG
Submitter Id	ERR9968821_bin.23_MetaWRAP_v1.3_MAG
assembly quality	Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software	metaspades v3.15.3
binning parameters	MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
binning software	MetaWRAP v1.3
broker name	EBI-EMG
completeness score	97.01
completeness software	CheckM
contamination score	1.42
geographic location (latitude)	33.8688
geographic location (longitude)	151.2093
local environmental context	oral cavity
metagenomic source	oral metagenome
sample derived from	SAMEA110373488
scientific_name	Actinomyces oris
sequencing method	Illumina NovaSeq 6000
taxonomic classification	The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinomycetia;o__Actinomycetales;f__Actinomycetaceae;g__Actinomyces;s__Actinomyces oris
taxonomic identity marker	multi-marker approach

Description: This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9968821 of study ERP139515.

BioProject: PRJEB65351
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Submission: EBI; 2023-09-29

Accession:: SAMEA114444320
ID:: 37603379

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Metagenome-Assembled Genome: ERR9968821_bin.23_MetaWRAP_v1.3_MAG

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