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Drosophila melanogaster adult testis library

Identifiers
BioSample: SAMN00156857; EST: LIBEST_002294
Organism
Drosophila melanogaster (fruit fly)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Ephydroidea; Drosophilidae; Drosophilinae; Drosophilini; Drosophila; Sophophora; melanogaster group; melanogaster subgroup
Attributes
strainy[*] w[67c1]/Y
sexmale
development stage1-5 day adult
lab hostSOLR (Stratagene)
tissuetestis
vectorpBlueScript SK (Stratagene)
v_typePlasmid
re_1EcoR I
re_2Xho I
Description

Testes dissected from 1-5 day adult y[*] w[67c1]/Y males raised at 25oC. RNA isolated using Trizol (Life Technologies) and a single round of Poly(A)+ selection using Oligotex (Qiagen). cDNA library constructed using Stratagene ZAP-cDNA syntesis kit. Oligo dT-primed, size fractionated ~1-6 kb, and directionally cloned at EcoRI and XhoI in Uni-ZAP XR. Following a single round of amplification pBlueScript SK phagemids were mass excised. A distribution channel for clones is being sought, but not currently available. Requests for clones cannot be honored.

Submission
NIDDK, National Institutes of Health, Brian Oliver; 1999-08-17
Accession:
SAMN00156857
ID:
156857

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