Format

Send to:

Choose Destination

Links from Nucleotide

NIH_MGC_58

Identifiers
BioSample: SAMN00160486; EST: LIBEST_005950
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissuehypernephroma
lab hostDH10B (T1 phage-resistant)
tissuekidney
vectorpDNR-LIB (Clontech)
v_typeplasmid (chloramphenicol resistant, grown at 30C)
re_1SfiI (ggccgcctcggcc)
re_2SfiI (ggccattatggcc)
Description

Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.35 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

Submission
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Ina Rolfs; 2000-09-13
Accession:
SAMN00160486
ID:
160486

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Support Center