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NCI_CGAP_Brn23

Identifiers
BioSample: SAMN00155902; EST: LIBEST_001318
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissueglioblastoma (pooled)
lab hostDH10B
tissuebrain
vectorpT7T3D-PacI
v_typephagemid (ampicillin resistant)
re_1Not I
re_2Eco RI
Description

1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATATCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo.

Submission
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Inge Arlart; 1998-06-22
Accession:
SAMN00155902
ID:
155902

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