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1st strand cDNA was primed with a Not I - oligo(dT)15 primer [5'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'], on mRNA from pooled 26 somite zebrafish embryos; double-stranded cDNA was ligated to Sal I adaptors (BRL), digested with Not I and cloned into the Not I and Sal I sites of the pSPORT1 vector (BRL). Library was constructed by Matthew Clark (Lehrach lab; ICRF, London and Max Planck Institut fuer Molekulare Genetik, Berlin) and was not biochemically normalised. 70,000 clones from this library were arrayed on high density filters and subsequently screened by oligonucleotide hybridization fingerprinting to identify unique or minimally redundant clones for more intensive analysis.
Nucleotide
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