NIH_BMAP_DJ2

Warning: The NCBI web site requires JavaScript to function. more...

Result Filters

Organism: Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus

Attributes

strain	C57BL/6
tissue	subfornical organ and postrema
development stage	Adult
lab host	DH10B (Life Technologies) (T1 phage resistant)
tissue	brain
vector	pT7T3-Pac (Pharmacia) with a modified polylinker
v_type	Plasmid
re_1	EcoR I
re_2	Not I

Description: UI-M-DJ2 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is: GCTACATGAT, subfornical organ and area postrema.